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Citations for Takara Bio :
3851 - 3900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Transformations were performed in Stellar competent cells (Takara). 2 μL of reaction was added to 50 μL Stellar Competent cells on ice for 30 mins followed by 45 second heat shock at 42 degrees C and resuspended in 500 μL SOC medium (Takara ...
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bioRxiv - Developmental Biology 2023Quote: ... All mESC lines were cultured in 2iLif in N2B27 (NDiff227 Takara Bio ...
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bioRxiv - Physiology 2023Quote: ... The cDNA of each mouse gene was amplified and detected using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO) and Thermal Cycler Dice Real Time System III (TAKARA BIO, Shiga, Japan). 18S ribosomal RNA was used for the reference gene ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently stained using a horseradish peroxidase (HRP)-conjugated monoclonal anti-StrepTag antibody (Iba) or mouse anti-6His (Clontech) monoclonal antibody combined with rabbit anti-mouse-HRP polyclonal antibodies (DAKO) ...
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bioRxiv - Molecular Biology 2023Quote: ... His6 (631212, Clontech, mouse, 1:500); NPL4 (sc-365796 ...
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bioRxiv - Microbiology 2023Quote: HEp-2 cells were transduced with supernatants of Plat-GP cells cotransfected with pMDG60 and pRetroX-Tet3G (TaKaRa), selected with 4 mg/ml G418 (Wako) ...
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bioRxiv - Microbiology 2023Quote: ... pAAV-UGI was constructed by cloning the entire coding sequence of UGI amplified from pBS-UGI-flag by PCR using the primers shown in S-Table 3 into the EcoRI and BamHI sites of pAAV-ZsGreen1 (TaKaRa).
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bioRxiv - Microbiology 2023Quote: ... pcDNA-EGFP-P2A-stop was constructed by amplifying the EGFP and P2A sequences from pEGFP-C1 (Clontech) using the primers shown in S-Table 3 and cloning it into the BamHI and EcoRI sites of pcDNA3.1(+ ...
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bioRxiv - Molecular Biology 2023Quote: ... was inserted in between the KpnI and HindIII sites of the pOPINK vector (42) using the In-Fusion HD Cloning Kit (Takara). The pOPINK vector incorporates an N-terminal GST tag and a 3C protease cleavage site into the PhPINK1 construct ...
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bioRxiv - Plant Biology 2023Quote: ... pDESTTM32RAPTOR1BcDNAwas co-transformed either with pDESTTM22FLZ14cDNA or pDESTTM22S6K1cDNA plasmids into the yeast strain Y2HGold (Takara Bio, USA) as previously described (Paiano et al. ...
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bioRxiv - Plant Biology 2023Quote: The Matchmaker Gold Yeast 1-hybrid system (Clontech) with the yeast one-hybrid (Y1H ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA fragment was inserted into the cloning site of pColdIII expression vector (TaKaRa Bio, Shiga, Japan). The constructed vector and the chaperone co-expression plasmid pGro1254 were introduced into E ...
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bioRxiv - Plant Biology 2023Quote: ... SRF mutants were prepared using PrimeSTAR Mutagenesis Basal Kit according to the manufacturer’s instructions (TaKaRa Bio, Shiga, Japan), and the mutations were confirmed by sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... and each was cloned into NdeI/SalI sites of the pRI201-AN vector (TaKaRa Bio, Shiga, Japan) to express under the control of the 35S promoter ...
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bioRxiv - Plant Biology 2023Quote: ... the resultant PCR products were inserted into the NdeI and KpnI restriction sites of the modified pColdIII expression vector (TaKaRa Bio, Shiga, Japan) containing the His6-tag sequence with stop codon (5’-catcaccatcaccatcactag-3’ ...
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bioRxiv - Plant Biology 2023Quote: ... the chimeric H5Indo gene was reamplified by PCR and then introduced in the T-DNA region of a pCAMBIA binary vector previously linearized with restriction enzymes SacII and StuI using the In-Fusion cloning system (Clontech). The same methods and PDI signal peptide were used to clone antibody chain genes ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant CML13 and CML14 were purified via Ni-NTA His60 (Takara Biosciences.) and CaM7 using phenyl-sepharose (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant AAV9 vectors were generated by co-transfection of AAVpro 293T cells (Takara Bio) with three plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then incubated with anti-GFP antibody (JL-8; Clontech) at a dilution of 1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... and the PrimeScript cDNA synthesis kit (TaKaRa, Shiga, Japan) with 400 ng RNA input in a 10 μl reaction ...
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bioRxiv - Microbiology 2023Quote: ... and reverse transcribed using the TaKaRa PrimeScript RNA Kit (cat# RR036A, Takara, Japan), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... coli competent cells were used (Takara, https://www.takarabio.com/). Finally ...
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bioRxiv - Systems Biology 2023Quote: ... following Yeastmaker Yeast Transformation protocol (Clontech). We verified transformants with colony PCR and sequencing a random set of ∼200 constructs.
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bioRxiv - Synthetic Biology 2023Quote: ... with 10% Tet Approved FBS (Clontech Laboratories or Avantor) and 1X Penicillin/Streptomycin (Life Technologies ...
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bioRxiv - Zoology 2023Quote: ... The PCR products were purified using Nucleospin Gel and PCR Clean-up (Takara Bio). Nucleotide sequence analysis was outsourced from a commercial sequencing service (Eurofins ...
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bioRxiv - Synthetic Biology 2023Quote: ... or SD-Trp Broth (Takara, 630411 and 630413) at 30°C with shaking at 225 rpm.
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bioRxiv - Synthetic Biology 2023Quote: ... and VL648-N were cultured in YPDA (Takara, 630464) or SD-Trp Broth (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were cloned into Gateway-compatible yeast two-hybrid vectors based on pGADT7 and pGBKT7 (Clontech) or into the pAG416GPD-ccdB-HA vector (Alberti et al. ...
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bioRxiv - Plant Biology 2023Quote: ... and a rabbit LexA binding domain antibody (Clontech), respectively.
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bioRxiv - Plant Biology 2023Quote: The gene encoding the PP1c1-293 protein (herein simply referred to as PP1c) was cloned into the pOPIN-SC3 vector (OPPF) via In-Fusion Cloning (Takara Bio), which provides a cleavable N-terminal 6xHIS-SUMO tag for purification and solubility ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... which had been made using the In- Fusion Snap Assembly (Takara) site-directed mutagenesis ...
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bioRxiv - Plant Biology 2023Quote: ... and the cDNA was obtained using the PrimeScript first Strand cDNA Synthesis Kit (Takara, Dalian, China). The open reading frames of genes were amplified by PCR from the cDNA of M ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and In-Fusion Snap Assembly (Takara) site-directed mutagenesis as described by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... using Primestar-GXL polymerase (Takara) and the following primers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Inducible expression of a Nef-eGFP fusion protein was established in CEM-T4 cells using the Lenti-XTM Tet-On System (Clontech now Takara Bio USA) which places expression of Nef-eGFP under the control of a doxycycline-inducible promoter ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized using a cDNA synthesis kit (TaKaRa, Dalian, China), and the sequencing adapter was linked to both ends ...
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bioRxiv - Plant Biology 2023Quote: ... by using In-Fusion HD Cloning Kit (Takara Bio). Amino acid substitutions were introduced into pMAL-c5X Raf13 by site-directed mutagenesis to produce the kinase-dead Raf13 (K546N) ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were cloned into pMD19-T vectors (TaKaRa, Japan). Different UGT amino acid sequences were downloaded from NCBI ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed TB Green Premix Ex Taq II (Takara) and the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Lenti-X-293T cells were purchased from Clontech Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... was performed by quantitative real-time polymerase chain reaction (PCR) using the AAVpro™ Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc), according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... First- strand cDNA was synthesized from total RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Shiga, Japan). PCR amplifications were performed using the KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Zoology 2023Quote: RNA was extracted from the hypothalamic infundibulum using Nucleospin RNA Plus XS (Takara Bio). PCR amplifications were performed using THUNDERBIRD SYBR qPCR Mix (TOYOBO) ...
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bioRxiv - Systems Biology 2023Quote: ... The GST/MBP expression vectors were constructed by amplifying the ORFs from the CLas yeast expression vectors and inserted between RE sites: BamHI and SalI in pGEX vector and XmnI and EcoRI in pMAL vector using infusion cloning (Clontech). These newly generated vector constructs were transformed into E ...
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bioRxiv - Plant Biology 2023Quote: ... following treatment with RNAase-free DNAase (Takara). RNA (1.5 μg ...
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bioRxiv - Pathology 2023Quote: ... cDNA was prepared from 1µg RNA using PrimeScriptTM RT reagent Kit with gDNA Eraser (TaKaRa). RT- qPCR was performed using 2.5 μL of cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated DNA was amplified by PCR using Hot Start TaKaRa LA Taq kit to yield a 10-Kb product (Takara Biotechnology, #RR042A). Primers utilized were the following ...
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bioRxiv - Bioengineering 2023Quote: ... (Takara Bio, Shiga, Japan). Data normalization was done with beta-2-microglobulin (β2M ...
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bioRxiv - Biochemistry 2023Quote: Lenti-X 293T cells (Takara) were seeded in 6-well plates (Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Mutants were generated by site-directed mutagenesis using a KOD-Plus Mutagenesis Kit (Toyobo, Japan) or PrimeSTAR® Mutagenesis Basal Kit (Takara Bio, Japan). For the expression ...