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Citations for Takara Bio :
3801 - 3850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The viral copies were quantitated by reverse transcription quantitative PCR following the handbook (Takara, Cat.#RR086A). The copy numbers of reverse-transcripts from cell cultures and mice organ samples were normalized with the expression level housekeeping gene of beta-actin by using the 2(-Delta Delta C(T) ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-DsRed (1:1000; TaKaRa #632496). The brain sections were washed three times with PBS before incubation in secondary antibody for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and the expression of specific gene was analysed using TB Green® Premix Ex Taq™ II (TaKaRa Bio Inc., Japan) in a Roche Light Cycler 480 with predesigned and validated primers from PrimerBank of Massachusetts General Hospital (61) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed in Thermal Cycler Dice Real Time System Lite (Takara Bio) using KOD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Cell Biology 2023Quote: ... The primers for In-fusion cloning (Takara Bio) Fstl1 are (Forward 5’-CACAGACGCGTACCGGTGCCACCATGTGGAAACGATGGCTGGCGCTC-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... A full-length Sfrp1 cDNA fragment was obtained by PCR amplifying mouse kidney cDNA using PrimeStar HS DNA polymerase (Takara Bio) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was generated from total RNA using the Prime Script 1st strand cDNA synthesis kit (Takara) according to the manufacturer’s recommended procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... the 3’ UTR of the virus were determined using the SMARTer®RACE 5’/3’ Kit (Takara Bio, Kusatsu, Japan). 2.4 µg of RGMoV gRNA was polyadenylated using Poly(A)polymerase (600 u/µl ...
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bioRxiv - Microbiology 2023Quote: ... using the In-Fusion system (Takara Clontech, Mountain View, CA) following manufacturer’s protocols48 ...
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bioRxiv - Microbiology 2023Quote: ... using the In-Fusion system (Takara Clontech, Mountain View, CA) following manufacturer’s protocols48 ...
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bioRxiv - Neuroscience 2023Quote: ... by In-Fusion recombinase (Clontech Laboratories). The ExRai-AKAR2 cDNA codon was optimized for Drosophila melanogaster (Eurofins Genomics) ...
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bioRxiv - Molecular Biology 2023Quote: ... 18 µl of Stellar (Takara Bio Europe SAS 636766) chemically competent bacteria were transformed using 2 µl of the ligation reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... anti GFP antibody (Clontech, lot. 1404005) and anti-H3 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... (Cat#639649, Takara Bio USA, Inc.) and incubated at 55 °C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: Various cell lines were treated with RNAiso Plus (Takara, Beijing, China) to extract total RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the isolated RNA (1 μg) was reverse transcribed into cDNA (20 μL) using PrimeScript™ RT Master Mix (Perfect Real Time, Takara). The qPCR reaction was initiated at 95 °C for 3 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The qRT-PCR was performed using TB Green® Premix Ex Taq (Takara, Tokyo, Japan) in three biological replicates on a CFX96 thermocycler (BIO-RAD ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was extracted and incubated with TALON affinity resin (Takara Bio) while rotating at 4 °C for 1 - 2 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... T4 DNA ligase and polymerase were from Takara (Shiga, Japan). Antibiotics were purchased from Oxford LTD (Hampshire ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated using NucleoZOL (Takara, Shiga, Japan, Cat. No. 740404.200) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: The in-fusion reaction using the HD in-fusion kit (Takara Bio) was carried out using 100ng of the cut vector ...
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bioRxiv - Microbiology 2023Quote: ... Fragments were joined via crossover PCR using PrimeStar Max (Takara Bio) per manufacturer instruction ...
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bioRxiv - Immunology 2023Quote: Lenti-X 293T (human embryonic kidney) cells were purchased from Takara Bio Inc ...
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bioRxiv - Immunology 2023Quote: ... The viral particles in the supernatant were then concentrated using the LentiX Concentrator (Takara Bio), in accordance with the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was prepared with SYBR qPCR Premix (Takara, Japan, RR420L), and then performed with a real-time PCR detection instrument (Bio–Rad CFX96 ...
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bioRxiv - Cancer Biology 2023Quote: ... monosodium salt (WST-1) (Takara BioInc, Clontech Laboratories, Inc.). 4 × 103 cells/ml was cultured in 5 replicates in 96-well plates ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... genomic DNA was isolated using Guide-it Mutation Detection Kit (Takara). DNA fragments flanking the targeted Drd1a locus and off-targets (Figure S4C ...
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bioRxiv - Neuroscience 2023Quote: In Fusion cloning (Takara Bio) was used to assemble the responder vector (p371 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μM Shield1 (Clontech lab, now Fisher Scientific) for 4 h ...
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bioRxiv - Microbiology 2023Quote: ... pSIREN-shRNA-LucIRR and pSIREN-shRNA GAPDHHB were purchased from Clontech. pCDNA3.1 was purchased from Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... pEGFP-N3 was from Clontech and pGEM-T Easy was from Promega (cat# A137A) ...
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bioRxiv - Cell Biology 2023Quote: In Fusion HD cloning (Takara 638910) was used to switch full length WT mouse Myo10 in EGFP-C1 to mScarlet-C1 ...
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bioRxiv - Genetics 2023Quote: ... rabbit anti-DsRED (Clontech #632496; 1:200), chicken anti-GFP (AVES ...
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bioRxiv - Genetics 2023Quote: ... RNA was first reverse-transcribed using Primescript 1st strand cDNA Synthesis kit (TaKaRa, #6110A) with random hexamers at 30°C for 10 mins ...
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bioRxiv - Microbiology 2023Quote: ... upstream and downstream flanking regions (approximately 1,000 bp) were amplified using PrimeStar HS (Takara Bio) and cloned into the vector pCE along with a 30 bp site specific spacer sequence (in the gene to be deleted ...
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bioRxiv - Immunology 2023Quote: ... the non-tissue culture 24-well plates were coated with 7 μg/ml RetroNectin (TAKARA) for 3 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... or pVAX by Infusion cloning (Takara). The GFP expression cassette hPGK-eGFP-BGHpA was amplified by PCR from plasmid CCR5-PGK-GFP.33 Antibody components (J3 ...
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bioRxiv - Developmental Biology 2023Quote: ... the TaKaRa DNA Ligation Kit LONG (TaKaRa Bio) was used along with NEB Stable Competent E ...
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bioRxiv - Microbiology 2023Quote: The following plasmids were used in this study and previously described: enhanced green fluorescent protein (EGFP) pEGFP-N1 (Clontech Laboratories, Mountain View, CA), pCI-neo HSV-2 UL21 (UL21 ...
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bioRxiv - Microbiology 2023Quote: The 16S rRNA gene sequences were amplified by PCR using LA Taq polymerase (TaKaRa-Bio, Inc., Shiga, Japan) using the primers Uni530F and Uni907R65 ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reactions were performed in triplicates in a final volume of 25 µL containing: TAKARA Ex Taq® buffer with MgCl2 (10 X; Takara Bio Inc., Tokyo, Japan), primers 200 nM ...
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bioRxiv - Microbiology 2023Quote: ... and DNase I (Takara Bio) on ice until the bacterial cell are lysed ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cells were harvested and suspended with xTractor™ buffer (Clontech) and then treated with appropriate amounts of proteinase inhibitors (Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... total RNA (1 ng) was injected into the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Palo Alto, CA, USA) for sequencing ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were transformed into Escherichia coli BL21 (Takara Bio Co Ltd, Japan) and the transformed cells were cultured at 37°C until the OD600 reached at 0.6–0.8 ...
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bioRxiv - Developmental Biology 2023Quote: ... This rtTA-advanced ORF was replaced with rtTA3G from the pCMV-Tet-3G plasmid (TaKaRa Bio), by XbaI and XmaI digestion and cloning of three separate fragments to create the final rtTA vector AAVS1-NEO-UBrtTA-3G.
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bioRxiv - Neuroscience 2023Quote: ... and protease inhibitors (Takara Bio, Kusatsu, Japan) mixes ...
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bioRxiv - Cell Biology 2023Quote: ... and microarray analysis were completed by Takara Bio Inc ...
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bioRxiv - Cell Biology 2023Quote: ... An amount of 1 μg RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara Biomedical Technology, China). For RT-qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μL of reaction was added to 50 μL Stellar Competent cells on ice for 30 mins followed by 45 second heat shock at 42 degrees C and resuspended in 500 μL SOC medium (Takara) and placed 1 hour shaking 200 rpm at 30 degrees C ...