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Citations for Takara Bio :
3751 - 3800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Yeast 2-hybrid (Y2H) analysis was performed using the protocols described in the Yeast protocols handbook (Clontech, Protocol No ...
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bioRxiv - Cancer Biology 2023Quote: ... were packaged in Lenti-X 293T Cells (Takara Bio, Cat. 632180) by transient transfection ...
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bioRxiv - Cell Biology 2023Quote: ... into pEGFP-N1 vector (Clontech). All PCRs were performed using Phusion High-Fidelity DNA Polymerase (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... the 3’ UTR of the virus were determined using the SMARTer®RACE 5’/3’ Kit (Takara Bio, Kusatsu, Japan). 2.4 µg of RGMoV gRNA was polyadenylated using Poly(A)polymerase (600 u/µl ...
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bioRxiv - Microbiology 2023Quote: ... using the In-Fusion system (Takara Clontech, Mountain View, CA) following manufacturer’s protocols48 ...
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bioRxiv - Microbiology 2023Quote: ... using the In-Fusion system (Takara Clontech, Mountain View, CA) following manufacturer’s protocols48 ...
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bioRxiv - Neuroscience 2023Quote: ... by In-Fusion recombinase (Clontech Laboratories). The ExRai-AKAR2 cDNA codon was optimized for Drosophila melanogaster (Eurofins Genomics) ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... The pLVSIN-EF1α-AcGFP-C1 vector (5.5 μg) was added to 7 μL Lentiviral Mix High Titer Packaging Mix (Takara Bio Inc., Otsu, Japan), 1500 μL serum-free DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNA sequences of CHIKV 6K and TF (Supplementary Figure S1) were cloned in the pEGFPC1 vector (Clontech) to generate fusion proteins tagged with the Enhanced Green Fluorescent Protein (EGFP ...
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bioRxiv - Cell Biology 2023Quote: The pik1-11 temperature sensitive allele was constructed as described (Tang et al., 2019) with the exception that EX taq polymerase (Takara, cat# 4025) and accompanying dNTPs (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... and accompanying dNTPs (Takara, cat# RR01BM) were used.
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bioRxiv - Cell Biology 2023Quote: ... The reporter cassette excised from pPExRF3 and the left and right homology arms were assembled and cloned into a linearized pUC19 in a single enzymatic reaction using the In-Fusion Cloning Kit (Clontech Laboratories Inc., California, U.S.A). A gRNA vector was constructed by cloning a 20-bp target sequence surrounding the stop codon of coronin into pU6 (FlyCRISPR) ...
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bioRxiv - Cell Biology 2023Quote: ... Lenti-X 293T cells were obtained from Takara Bio and cultured in DMEM (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and microarray analysis were completed by Takara Bio Inc ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of total RNA was used to make cDNA libraries using prime script RT reagent kit gDNA eraser (Takara Bio Inc.) according to the kit’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: DNA template for RNA synthesis was created using the forward and reverse primers listed below with GXL prime star PrimeSTAR® GXL DNA Polymerase (Takara Cat# R050A). mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (ThermoFisher Cat# AM1345 ...
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bioRxiv - Developmental Biology 2023Quote: ... using In-Fusion cloning (Takara Bio, primers are listed in Table S3) and then inserted into a pDestTol2ACryGFP backbone (Berger and Currie ...
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bioRxiv - Biochemistry 2023Quote: ... by RT-PCR (Takara, RR014) and cloned by Gibson assembly into the vector pK27SUMO ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were collected at 36 and 72 h and then purified with 10% Lenti-X Concentrator® (Clontech, Mountain View, CA, USA). The virus copy number was determined by Q-PCR ...
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bioRxiv - Cancer Biology 2023Quote: Using Ex Taq DNA Polymerase (Takara), sgRNA inserts from each initial and final pool were PCR-amplified from 144 μg of gDNA to achieve ∼400-fold coverage of the library ...
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bioRxiv - Cell Biology 2023Quote: ... An amount of 1 μg RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara Biomedical Technology, China). For RT-qPCR ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified from Escherichia coli BL21 (DE3) with TALON Metal Affinity Resin (Takara), Pierce Glutathione Agarose (Thermo scientific) ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid assays were performed using strain AH109 (Takara). Vectors pGADT7 and pGBKT7 were sequentially transformed into this strain ...
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bioRxiv - Plant Biology 2023Quote: Y1H assay was conducted according to protocol (Clontech, cat. No. 630491). Briefly ...
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bioRxiv - Plant Biology 2023Quote: Y2H assay was performed according to protocol (Clontech, cat. No. 630489). Since BcICE1 self-activates ...
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bioRxiv - Plant Biology 2023Quote: ... and then 1 mL of RNAiso Plus (Takara Bio., Japan) was added and thoroughly mixed ...
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bioRxiv - Plant Biology 2023Quote: ... TB GreenTM Premix Ex TaqTM (Takara) was used as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The full-length was also cloned into the lentiviral vector pLVX-TetOne-Puro (Clontech, Takara, #631849). The plasmid pECMV-3×FLAG-RAB11A was purchased from SinoBiological (Beijing ...
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bioRxiv - Neuroscience 2023Quote: ... DCX: doublecortin (C-18) goat polyclonal IgG (1:200, Takara Bio, Cat# 632496). NeuN ...
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bioRxiv - Neuroscience 2023Quote: ... and Living Colors® DsRed Polyclonal Antibody (1:200, Clontech). BrdU ...
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bioRxiv - Plant Biology 2023Quote: All yeast work was performed according to the yeast protocols handbook (Clontech, Mountainview, CA). For Y2H screening ...
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bioRxiv - Plant Biology 2023Quote: The full-length MpACL5 amplified from Tak-1 cDNA by PCR using TaKaRA EX Taq (Takara, Shiga, Japan) with the primers ...
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bioRxiv - Plant Biology 2023Quote: ... and gametandiopore of one-month-old Tak-1 and Tak-2 by NucleoSpin RNA Plant (Takara) or Monarch Total RNA Miniprep Kit (New England BioLabs ...
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bioRxiv - Biochemistry 2023Quote: ... EPHA2- FN2 and antigen-A were purified using His 60 Ni Superflow resin (Takara), whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... RNA was extracted and libraries prepared using SMARTer Stranded RNA-Seq Kits according the manufacturer’s protocols (Takara) by the Integrated Genomics Operation (IGO ...
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bioRxiv - Plant Biology 2023Quote: ... under the control of cauliflower mosaic virus 35S promoter in the binary vector pBI121 (Clontech, USA) was transferred into Agrobacterium tumefaciens strain GV3101 and used to transform Arabidopsis by floral dip method and tobacco plants by leaf disk method (Horsch et al. ...
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bioRxiv - Microbiology 2023Quote: ... GISAID ID: EPI ISL 408667) [30] were generated by PCR using PrimeSTAR GXL DNA polymerase (TAKARA, Cat# R050A). A linker fragment comprising hepatitis delta virus ribozyme ...
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bioRxiv - Microbiology 2023Quote: ... mRNA was reverse transcribed into cDNA using the PrimeScript cDNA synthesis kit (Takara). The following primer pairs were used for gene amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were lysed with the xTractor Bacterial Cell Lysis Buffer (Clontech) and sonicated ...
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bioRxiv - Immunology 2023Quote: ... The DNA product was purified from the gel slice using the PCR cleanup and gel extraction kits (740609.50, Takara Bio, Kusatsu, Shiga). The purified DNA was cleaned using AMPure XP (A63881 ...
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bioRxiv - Biochemistry 2023Quote: ... 1000 ng of RNA was used to synthesize cDNA by reverse transcriptase (Takara, #RR037A). The cDNA was diluted with DNase/RNase-free water 10-fold before performing qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary goat anti-dsRED (1:1000, Takara Bio, Cat# 632496, RRID: AB_10013483), goat anti-ChAT (1:400 ...
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bioRxiv - Cell Biology 2023Quote: shRNA constructs were engineered on a pSIREN retroviral vector (Clontech). To deplete the endogenous expression of BIN1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (634898, Takara Bio.). The cDNA quality was examined on Agilent TapeStation system (RRID:SCR_019547 ...
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bioRxiv - Neuroscience 2023Quote: ... and then the virus solution was concentrated using the Lenti-X Concentrator (631231, Clontech) and suspended in PBS (in vivo conditions).
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bioRxiv - Neuroscience 2023Quote: ... and concentrated to 50x in 1xPBS using Lenti-X Concentrator (Clontech #631232) in accordance with the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: The CREB Dominant-Negative Vector Set was purchased from Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: The helper plasmid and the AAV-ITR plasmid with a reporter gene (pAAV-ZsGreen1) were acquired from Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... The approximately 1500bp amplicons were run through agarose gel electrophoresis to ensure a lack of secondary structure formation within the sample and DNA was purified from agarose gel (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA was isolated with MiniBEST Universal RNA extraction Kit (TaKaRa). 500 ng total RNA was saved as input sample ...