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Citations for Takara Bio :
3651 - 3700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: RNAiso PLUS (Cat. No. 9109, Takara) was used to isolate total RNA from the cells ...
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bioRxiv - Immunology 2023Quote: ... cDNA was then used for qPCR analysis using the TB Green® Premix Ex Taq™ II (Ti RNase H Plus) (Cat. No. RR820A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells (live singlet CD19+ CD4− IgDlo CD71+CD38int CD20+ CXCR5+) were sorted using a FACSAria II into 96-well plates containing 2 μL Lysis Buffer (Clontech) supplemented with 1 U μl−1 RNase inhibitor (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was collected by centrifugation at 35,000 x g for 30 min and incubated with TALON IMAC resin (Clontech) and 20mM imidazole at 4 °C overnight.
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bioRxiv - Immunology 2023Quote: ... The fragments were gel purified and then assembled into a plasmid using in-fusion reagents (Takara Bio). The mCherry11-RNase L and mCherry1-10-RNase L were made similarly with oligos in Supplementary table 1 ...
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bioRxiv - Genomics 2023Quote: ... After treatment of DNase I (Takara Bio), the synthesized guide RNA was purified using the RNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Genomics 2023Quote: ... purified RNA were constructed with the SMARTer Stranded RNA-seq Kit (Takara, cat. no. #634839) according to the manufacturer’s instructions for first- strand cDNA synthesis ...
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bioRxiv - Genomics 2023Quote: ... GenTLE Precipitation Carrier (Takara, #9094) and centrifuged for 15 min at 12000 r.p.m ...
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bioRxiv - Genomics 2023Quote: ... All PCR were performed using PrimeSTAR Max DNA polymerase (Takara, Japan). The DGP-4 fragments were then cloned into the AscI/NheI (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... and the cells were added with RNAiso Plus (Takara Bio, Japan) to digest ...
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bioRxiv - Developmental Biology 2023Quote: ... where the Hygromycin cassette was replaced by the Zeocin selection cassette using In-Fusion cloning (Takara). After one day of recovery ...
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bioRxiv - Developmental Biology 2023Quote: ... and inserted with the fragment of pBI-TRE (Clontech) containing the bidirectional tetracycline-responsive element (TRE ...
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bioRxiv - Developmental Biology 2023Quote: ... and subcloned into the EcoRI site of pT2A-BItight-TRE by In-Fusion® HD cloning kit (TaKaRa). pT2A-BItight-Lifeact-mCherry-TRE ...
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bioRxiv - Cell Biology 2023Quote: The Tet-On adenoviral cosmid vector was produced using the following procedure: the multicloning site of the pTetOne vector (TaKaRa) was modified to retain a SwaI recognition sequence ...
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bioRxiv - Cell Biology 2023Quote: ... and the excised DNA was purified and transfected into Adeno-X 293 cells (TaKaRa). When late CPE was observed ...
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bioRxiv - Cell Biology 2023Quote: ... Library quantification was carried out using a next generation sequencing (NGS) library quantification kit from Takara Bio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 300 ng was used as template for the reverse transcription using the PrimeScriptTM RT Master mix (#RR036A, Takara, France). The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272 ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA from these retained samples was reverse-transcribed into cDNA using 250 ng as a template and the PrimeScriptTM RT Master mix (#RR036A, Takara, France). To provide a comparison point for the allele-specific expression in F1 larvae ...
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bioRxiv - Developmental Biology 2023Quote: K14-HA-Fzd6-tdTomato constructs were generated by using In-Fusion (Takara) cloning to first add the tdTomato tag from pBluescript (Stratagene ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-dsRed (1:500, Takara Bio), guinea pig anti-Sdk (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... A vector was constructed by fusing three PCR amplicons as described below using In-fusion cloning (Takara) and used for homologous recombination ...
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bioRxiv - Cell Biology 2023Quote: ... 3xFLAG-SMARCAL1 cDNAs were cloned into the lentiviral vector pLVX-TetOne-Puro (Clontech) by Gibson Assembly ...
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bioRxiv - Developmental Biology 2023Quote: ... HpCTCF cDNA was amplified by RT-PCR with TaKaRa LA-taq (TaKaRa Bio Inc., Japan) using the following primers (5’-GTTCGCCATTCATTTGAGTGGATATTTC-3’ and 5’- CTGATCAGGTAAACTCTAAATCAGATTCC- 3’).
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bioRxiv - Developmental Biology 2023Quote: ... and LIF in a base medium of NDiff227 (Takara, Y40002) (Ying et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Sorted cells (>50,000 cells) were cultured on 48-well tissue culture plates coated with RetroNectin (r-fibronectin CH-296; Takara Bio Inc.) in EC media ...
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bioRxiv - Developmental Biology 2023Quote: ... and cloned into E.coli DH5α competent cells (Takara). Plasmids were extracted from bacterial cultured overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Nested PCR was performed with rTaq (Takara), followed by the PCR product fragments sequencing with Sanger sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... At 48 hpf the GFP + progeny was genotyped using a forward primer in the foxo1a endogenous locus and a reverse primer in the EGFP inserted transgene using a touch-down PCR with the Terra™ PCR Direct Polymerase Mix (639270; Takara). The PCR product was run in a 1% agarose gel to validate its size and the remaining product was sent for Sanger sequence.
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bioRxiv - Cell Biology 2023Quote: ... The primers for In-fusion cloning (Takara Bio) Fstl1 are (Forward 5’-CACAGACGCGTACCGGTGCCACCATGTGGAAACGATGGCTGGCGCTC-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... A full-length Sfrp1 cDNA fragment was obtained by PCR amplifying mouse kidney cDNA using PrimeStar HS DNA polymerase (Takara Bio) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc., Otsu, Japan).
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: Using the Lenti-X 293 T cell line (Takara Bio Inc., Otsu, Japan) and the pLVSIN-EF1α-AcGFP-C1 vector ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... and 45 μL Trans IT-293 Transfection Reagent (Takara Bio Inc., Otsu, Japan). After 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... Shield1 (632189) (TAKARA); colcemid (15212-012 ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of EPHX2 was amplified by PCR from liver cDNA (Human Multiple Tissue cDNA panels; Takara Bio, Shiga, Japan) using primers (5’-GTCGACATGACGCTGCGCGCGGCCGTCTTCG-3’ ...
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bioRxiv - Genomics 2023Quote: ... The first-strand cDNA was amplified with PrimeSTAR GL DNA polymerase (Clontech) to generate double-stranded cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... The copy number was calculated using the standard curves for cloned Tert and Dloop in a pMD19-T Easy vector (TaKaRa, 3271), respectively ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was generated from total RNA using the Prime Script 1st strand cDNA synthesis kit (Takara) according to the manufacturer’s recommended procedure ...
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bioRxiv - Cancer Biology 2023Quote: ... and developed using Western BLoT Chemiluminescent HRP Substrate (Takara). The chemiluminescent images were observed using FUSION FX (Vilber ...
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bioRxiv - Cancer Biology 2023Quote: ... detected with Western BLoT Rapid Detect v2.0 (Takara, Shiga, Japan), and developed using Western BLoT Chemiluminescent HRP Substrate (Takara) ...
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bioRxiv - Cancer Biology 2023Quote: PDGF-IRES-Cre was generated by cloning human PDGF-B and Cre into pQXIX vector (Clontech) as previously described5 ...
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bioRxiv - Cancer Biology 2023Quote: ... quantified with Lenti-X GoStix Plus (#631280, Takara Bio, Japan), aliquoted and stored at -80°C.
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bioRxiv - Developmental Biology 2023Quote: ... All mESC lines were cultured in 2iLif in N2B27 (NDiff227 Takara Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μL of reaction was added to 50 μL Stellar Competent cells on ice for 30 mins followed by 45 second heat shock at 42 degrees C and resuspended in 500 μL SOC medium (Takara) and placed 1 hour shaking 200 rpm at 30 degrees C ...
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bioRxiv - Cancer Biology 2023Quote: ... Transformations were performed in Stellar competent cells (Takara). 2 μL of reaction was added to 50 μL Stellar Competent cells on ice for 30 mins followed by 45 second heat shock at 42 degrees C and resuspended in 500 μL SOC medium (Takara ...
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bioRxiv - Cancer Biology 2023Quote: ... The transduced cells were selected using puromycin (1 μg/ml; Clontech) for five days ...
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bioRxiv - Cancer Biology 2023Quote: ... LipofectamineTM 2000 kit and reverse transcription kit were purchased from TaKaRa Company ...
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bioRxiv - Biochemistry 2023Quote: ... or the EmeraldAmp GT PCR Master Mix (Takara Bio). Primers were obtained from Merck or Eurofins Genomics ...