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Citations for Takara Bio :
3601 - 3650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse transcribed using the PrimeScript RT Reagent Kit (Takara). RT-qPCR was performed with TaqMan Gene Expression Master Mix (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-DsRed (1:1000, Clontech #632496), mouse anti-RFP (Invitrogen (RF5R) ...
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bioRxiv - Cell Biology 2023Quote: ... QPCRs was detected with SYBR Green PCR Master Mix (Takara, Japan) reagent ...
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bioRxiv - Immunology 2023Quote: ... total RNA underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), and amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... the calreticulin signal sequence of pDsRed2-ER (Clontech) was removed by cleavage with NheI and BglII ...
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bioRxiv - Bioengineering 2023Quote: ... An RNA purification kit (NucleoSpin®) was purchased from TAKARA (Shiga, Japan). Dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cell Biology 2023Quote: ... All other constructs were generated by In-Fusion Cloning (Takara, Saint-Germain-en-Laye, France) using the manufacture’s instructions ...
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bioRxiv - Genetics 2023Quote: DNA amplification was performed using PCR with PrimeSTAR GXL (Takara) using standard protocols and 35 cycles unless otherwise specified ...
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bioRxiv - Cancer Biology 2023Quote: ... A bead ratio of 1x was used (50 µL of AMPure XP beads to 50 µL cDNA PCR product with 1 µL of 10x lysis buffer added, as per Clontech instructions), and purified cDNA was eluted in 17 µL elution buffer provided by Clontech ...
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bioRxiv - Molecular Biology 2023Quote: ... 18 µl of Stellar (Takara Bio Europe SAS 636766) chemically competent bacteria were transformed using 2 µl of the ligation reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... anti GFP antibody (Clontech, lot. 1404005) and anti-H3 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... (Cat#639649, Takara Bio USA, Inc.) and incubated at 55 °C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... the total RNA of three samples in each group was extracted with the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan), and linear RNA was then removed with Rnase R (GENESEED ...
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bioRxiv - Molecular Biology 2023Quote: ... The TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan) was used to extract the total RNA from the total RNA of larval gut samples in the AcCK1 ...
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bioRxiv - Molecular Biology 2023Quote: Various cell lines were treated with RNAiso Plus (Takara, Beijing, China) to extract total RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the isolated RNA (1 μg) was reverse transcribed into cDNA (20 μL) using PrimeScript™ RT Master Mix (Perfect Real Time, Takara). The qPCR reaction was initiated at 95 °C for 3 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The qRT-PCR was performed using TB Green® Premix Ex Taq (Takara, Tokyo, Japan) in three biological replicates on a CFX96 thermocycler (BIO-RAD ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was extracted and incubated with TALON affinity resin (Takara Bio) while rotating at 4 °C for 1 - 2 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... T4 DNA ligase and polymerase were from Takara (Shiga, Japan). Antibiotics were purchased from Oxford LTD (Hampshire ...
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bioRxiv - Molecular Biology 2023Quote: ... A mitochondrial DNA monitoring primer set kit from Takara Bio [San Jose ...
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bioRxiv - Molecular Biology 2023Quote: ... For quantitative RT-PCR 1ug of total RNA was treated with 5 Units of DNase I (Takara) and cDNA synthesized with SuperScriptIII (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... as well as a stock of pmCherryC1 mammalian expression vector (Clontech) was digested using BglII and SalI (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNAs were analyzed using Premix Ex Taq (Takara) in qPCR experiments ...
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bioRxiv - Cell Biology 2023Quote: ... Clone and sequence using the pMD18-T vector (TaKara).
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bioRxiv - Cell Biology 2023Quote: ... which was then converted into cDNA using ReverTra Ace (Toyobo) and oligo-dT (Takara). The resulting cDNAs were analyzed using Premix Ex Taq (Takara ...
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bioRxiv - Bioengineering 2023Quote: ... Using a BCA protein kit (TaKaRa, Shiga, Japan), a working solution (BCA reagent A ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were mixed with lentivirus at multiplicity of infection (MOI) equal to 5 in Retronectin (Takara, T100B)-coated culture plates and centrifuged at 1800 g for 1 h at 32 °C for lentiviral transduction before returning to normal culture condition ...
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bioRxiv - Bioengineering 2023Quote: ... The virus titer was determined using AAVPro titration kit (Takara).
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae was carried out by using the lithium acetate method of Yeastmaker™ Yeast Tansformation System (CLONTECH Laboratories, Inc.). The plasmid of pICASA was cleaved by XbaI and integrated into the chromosomal DNA of S ...
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bioRxiv - Bioengineering 2023Quote: ... DNA fragments were amplified with the high-fidelity polymerase PrimeSTAR Max DNA Polymerase from TaKaRa and colony PCR reactions were performed using DreamTaq polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentrated using Lenti-X Concentrator (Takara Bio 631232). For CRISPR/Cas9 gene knockout ...
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bioRxiv - Cancer Biology 2023Quote: ... water-soluble tetrazolium salt (WST-1) (Takara, India). The western diet was customized and purchased from Research Diets Inc ...
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bioRxiv - Biochemistry 2023Quote: ... into which a PCR-amplified DNA fragment containing the mNeonGreen gene (Shaner et al., 2013) was inserted using the in-Fusion reaction (Clontech). To generate pRS426-CPY(1-50)-ATG15(Δ1-35)-mNeonGreen (YPL073) ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Wild-type (WT) TIPRL1 cDNA was cloned into 3xFLAG pLenti6.4 using In-Fusion® HD Cloning Kit (Takara, #639650). Phosphomimic and non-phospho mutants (S265D and S265A ...
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bioRxiv - Biochemistry 2023Quote: ... using CalPhos Mammalian Transfection Kit (Clontech®/Takara, #631312) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Inc. PrimeSTAR HS polymerase (cat. R40A) was purchased from Takara. Polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... and primers containing approximately 500 base pairs sequence homologous to the target locus and then cloned into the EcoRV site of pBluescript SK+ using an In-Fusion HD Cloning Kit (Takara Bio). TP53-missense mutations and protospacer adjacent motif (PAM ...
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bioRxiv - Cell Biology 2023Quote: ... lenti-X 293T Cells (Takara Bio) were co-transfected with Lentiviral High Titer Packaging Mix (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... using an In-Fusion HD Cloning Kit (Takara Bio). To produce lentiviral stocks ...
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bioRxiv - Biochemistry 2023Quote: ... 1,5 µg of plasmid DNA and 1,5 µg pOG44 (encoding the Flp recombinase) were mixed and transfected using CalPhos Mammalian Transfection Kit (Clontech®/Takara, #631312). The transfection medium was replaced by complete DMEM after 24 h ...
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bioRxiv - Biochemistry 2023Quote: ... 1,5 µg of plasmid DNA and 1,5 µg pOG44 (encoding the Flp recombinase) were mixed and transfected using CalPhos Mammalian Transfection Kit (Clontech®/Takara, #631312). The transfection medium was replaced by complete DMEM after 24 h ...
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bioRxiv - Microbiology 2023Quote: ... or TransIT-293 (Takara, Cat# MIR2700) with Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the nine pmW118 plasmid vectors were subjected to amplification of the cDNA fragments (F1-F9-10) of SARS-CoV-2 XBB.1 and XBB.1.5 by PrimeSTAR GXL DNA polymerase (Takara) with the primer sets24 ...
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bioRxiv - Microbiology 2023Quote: ... The nine fragments of SARS-CoV-2 and the UTR linker for SARS-CoV-2 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara). After gel purification of the fragments ...
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bioRxiv - Microbiology 2023Quote: ... coli StellarTM (Takara Biosciences) was used for cloning and plasmid storage ...
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bioRxiv - Biochemistry 2023Quote: ... and a fusion construct containing nucleotides of CPY (1-50) and Atg15ΔN35 was generated by ligation (Clontech). To generate pRS426-ATG15-3xFLAG (TPL003) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated overnight with 0.5 ml of Talon (immobilised metal affinity chromatography, IMAC) resin (Clontech) in the presence of 150 mM NaCl and 20 mM imidazole ...
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bioRxiv - Bioengineering 2023Quote: ... CCR5-KO donor rAAV6 virus was produced in HEK-293T cells and was purified with AAVpro Purification Kit (TakaRa, San Jose, CA, USA). Electroporation of the RNP complex was performed using the Lonza 4D-Nucleofector (Lonza Group Ltd ...
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bioRxiv - Biochemistry 2023Quote: ... Ex Taq polymerase (Takara Bio, catalog no. RR01CM) and the PCR1 primer pair listed below ...
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bioRxiv - Immunology 2023Quote: ... cDNA synthesis from total RNA using the Prime Script RT Reagent Kit (Cat. No. RR047A, Takara) according to the manufacturer’s instructions ...