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Citations for Takara Bio :
3551 - 3600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... site-directed mutagenesis was performed using a PrimeSTAR mutagenesis basal kit (Takara Bio) with the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 × 106 HEK293T cells were transfected with 7 μg of Env expressor and 1 μg of a green fluorescent protein (GFP) expressor (pIRES2-EGFP; Clontech) with the calcium phosphate method ...
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bioRxiv - Molecular Biology 2023Quote: ... site-directed mutagenesis was performed by using a PrimeSTAR mutagenesis basal kit (Takara Bio) and pEGFP-C1-mADAR1 p150 as a template with the following primers ...
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bioRxiv - Genomics 2023Quote: ... 4 µl of Dr.GenTLE precipitation carrier (Takara, cat. no. 9094) and 4 µl sodium acetate ...
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bioRxiv - Genomics 2023Quote: ... 2.5 U Takara Epi Taq HS (Takara, cat. no. R110A, 5 U/µl), 2.5 mM MgCl2 ...
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bioRxiv - Genomics 2023Quote: ... Lentivirus containing the overexpression plasmid was generated using LentiX Packaging Single Shots (Clontech, 631278) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... All the mutant constructs of CE were prepared from pcDNA4/TO-bio-myc-mCE by using In-Fusion Site Directed Mutagenesis kit (Takara Bio). mCherry-H2B ...
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bioRxiv - Molecular Biology 2023Quote: ... The Matchmaker two-hybrid system 3 (Clontech Laboratories, Mountain View, CA, USA) was used for the yeast two-hybrid assay ...
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bioRxiv - Molecular Biology 2023Quote: ... were constructed by cloning the full-length coding region into the pGADT7 vector using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). The Matchmaker two-hybrid system 3 (Clontech Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... Cytotoxicity was assessed in parallel using the LDH Cytotoxicity Detection Kit (Takara Bio Inc.)
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bioRxiv - Genomics 2023Quote: ... The obtained PCR products were purified using NucleoSpin Gel and PCR Cleanup kit (Takara Bio, Shiga, Japan) and sequenced directly or were cloned into the pTAC-1 vector (Biodynamics ...
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bioRxiv - Genomics 2023Quote: ... The RT-PCR products were treated with NucleoSpin Gel and PCR Cleanup kit (Takara Bio) and directly analyzed using Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Genomics 2023Quote: ... 5 µm-thick sections were cut and immunostained with anti-GFP antibody (living colors, Clontech, 632592) and secondary anti-rabbit IgG with Alexa fluor 488 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... and enzymatic digestion and ligation (TaKaRa, Japan) systems ...
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bioRxiv - Genomics 2023Quote: ... while the out/out PCRs were carried out with PrimeSTAR GXL DNA Polymerase (Takara Bio, R051A). Seamless HDR was confirmed by sequencing the junctions between the insert and the mouse genome ...
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bioRxiv - Cancer Biology 2023Quote: ... non-adherent plate (Takara Bio). Cells were grown for 14 days ...
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bioRxiv - Biophysics 2023Quote: ... using Bsp1407I (TaKaRa, Shiga, Japan) and inserted into mCherry-C1 (mCherry-TIA1).
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: ... Resulting oligos were then annealed together and cloned into the pSIREN-RetroQ vector (TaKaRa) according to the manufacturer’s protocol between BamHI and EcoRI cloning sites.
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bioRxiv - Cell Biology 2023Quote: ... BsrGI digested and ligated into a likewise digested C1 (Clontech) backbone ...
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bioRxiv - Molecular Biology 2023Quote: ... Twenty microliter reactions were analyzed as outlined by the manufacturer (TaKaRa). The relative fold-changes of the transcripts were calculated using the comparative CT(2-ΔΔCT ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1 μg of total RNA was reverse-transcribed with random primers using the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was conducted using TB Green fluorescence (TaKaRa) on a LightCycler 480 II System (Roche Diagnostics ...
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bioRxiv - Genetics 2023Quote: ... rabbit anti-dsRed (Takara Bio-632393; 1:300), rabbit anti-PER (Rosbash lab ...
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bioRxiv - Neuroscience 2023Quote: ... 200uM dNTPs (Takara), 250nM primer-F ...
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bioRxiv - Synthetic Biology 2023Quote: ... and PrimeSTAR® GXL DNA Polymerase (TaKaRa) were used for PCR reactions to produce the DNA parts for genome modifications ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan); PrimeScript RT-PCR Kit (Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... 500 ng of RNA was used for cDNA synthesis using a PrimeScript RT Reagent Kit (Takara, RR037A). 2 μL of diluted cDNA (1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... then cDNA was synthesized using the SMART-Seq® v4 Ultra® Low Input RNA kit (Takara Bio USA Inc. Cat No. 634888). Libraries were prepared using the NexteraTM DNA Flex Library Prep (Illumina Cat No ...
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bioRxiv - Developmental Biology 2023Quote: ... or NucleoSpin RNA Plus XS (Takara Bio) kit ...
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bioRxiv - Cell Biology 2023Quote: ... 500nM AP21967 (Takara) was added to the media at least 30 minutes prior to imaging.
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bioRxiv - Biophysics 2023Quote: Plasmids used in this study (Supplementary Table S1) were assembled by Polymerase Chain Reactions (PCR) amplifying insert and vector DNA fragments followed by In-Fusion cloning (Takara Biosciences, In-Fusion HD Cloning Kit). Oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... with 10% foetal bovine serum (tetracycline-free FBS, Takara Bio) and 1.2 μg ml−1 puromycin (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... with 5% foetal bovine serum (tetracycline-free FBS, Takara Bio) and 10 U mL−1 penicillin and 10 μg mL−1 streptomycin (Pen-Strep ...
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bioRxiv - Cancer Biology 2023Quote: ... iCaspase-T2A-tdTomato sequence was removed and swapped for mNeonGreen-NLS using a combination of Q5® High-Fidelity DNA Polymerase and the In-Fusion HD Cloning kit (Takara). Final targeting vector was generated by insertion of homology arms (∼600bp each ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab5A (pJV0054) was generated similarly: cDNA was cloned via PCR using primeSTAR max DNA polymerase (Clontech). This was followed by subcloning into a zero blunt TOPO II vector (Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... TetON regulated transgenes were activated by 2μg/ml doxycycline (Clontech #631311) administration ...
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bioRxiv - Developmental Biology 2023Quote: ... Purified total RNA was reverse transcribed into cDNA by PrimeScript II 1st strand cDNA Synthesis Kit (Takara, 6210A) or SMARTer™ RACE cDNA Amplification Kit (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... 2019) and ACOT8 (ACOT8 H78A) (Ishizuka et al., 2004) were constructed by PCR-mediated mutagenesis using PrimerSTAR DNA polymerase (Takara). cDNAs for proteins expression were constructed in pLV cs2.0 vectors ...
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bioRxiv - Biophysics 2023Quote: ... or the InFusion cloning kit (Takara Bio) and the corresponding primer sets (Table S1) ...
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bioRxiv - Developmental Biology 2023Quote: ... prior to cells being fully resuspended to a single-cell suspension in 2mL NDiff227 with a 1mL pipette (Takara). Using a multichannel pipette ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/ml Doxycycline (Dox) (Clontech) was added into PGC-cultured FAcs medium 24 hours before the injection ...
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bioRxiv - Developmental Biology 2023Quote: ... and fluorescence quantitative kit (Takara Bio, Japan). The primer sequence is the same as Table 1 mentioned in 2.7
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR detection was performed on each group of samples by using a reverse transcription kit (Takara Bio, Japan) and fluorescence quantitative kit (Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... Polyadenylated RNA-seq libraries were prepared by using SMART-Seq v4 PLUS Kit (Clontech, Mountain View, CA, USA). Indexed RNA-seq libraries were sequenced using Illumina HiSeqX sequencer (paired end ...
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bioRxiv - Developmental Biology 2023Quote: ... lentivirus was first packaged following protocols we described previously1 and was then concentrated 100x using Lenti-X Concentrator kit (Takara, # 631231). Concentrated lentivirus was aliquoted and stored in -80°C before use ...
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bioRxiv - Developmental Biology 2023Quote: ... the PCR products were subcloned into EcoRV site of pBluescript SK- vector using In-Fusion® HD Cloning Kit (TaKaRa Bio, Japan) and sequenced with the M13 forward primer (5’-GTAAAACGACGGCCAG-3’ ...
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bioRxiv - Developmental Biology 2023Quote: ... primary antibodies (chicken anti-GFP, Abcam, ab13970, 1:2000 and rabbit anti-DsRed, Clontech, 632496, 1:200) were incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μl of cell extract was used in a buffer containing final concentrations of 52 mM HEPES pH 7.4 (Takara), 35 mM KGlu (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... the expression of GADD34Δ and K3L was induced by adding 1 µg/ml of doxycycline (Takara). After one additional day ...
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bioRxiv - Plant Biology 2023Quote: ... Two biological replicates were processed for next-generation library preparation using the ThruPLEX DNA-seq kit (Takara). Next ...