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Citations for Takara Bio :
3101 - 3150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... each for a 50-µL reaction containing ∼30 fmol of EcoRI-XbaI digested pLVSIN-CMV-Pur backbone plasmid (Takara #6183), ∼300 fmol of the insert fragment ...
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bioRxiv - Systems Biology 2023Quote: ... The total cfRNA library was prepared by SMARTer® Stranded Total RNA-Seq Kit – Pico (TaKaRa). Libraries were sequenced on Illumina HiSeq X-ten (~37.5 million paired-end reads per library ...
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bioRxiv - Systems Biology 2023Quote: ... Recombinant DNase I (TaKaRa) was used to digest DNAs ...
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bioRxiv - Systems Biology 2023Quote: ... The PBMC RNA library was prepared by SMARTer® Stranded Total RNA-Seq Kit – Pico (TaKaRa). All libraries were sequenced on Illumina HiSeq X-ten (~38.8 million per library ...
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bioRxiv - Plant Biology 2023Quote: ... with ROX as reporter (TakaRa). Three biological replicates were used for each of the above-mentioned sample type ...
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bioRxiv - Plant Biology 2023Quote: ... the promoter fragment was amplified and cloned between HindIII and XbaI sites using In-Fusion® HD Cloning Kit (Takara), generating pGWB502-HSP90.7pro ...
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bioRxiv - Plant Biology 2023Quote: ... T-DNA insertions were then analysed using specific primers (Table S1) in PCR reactions with Emerald Master Mix (Takara). PCR conditions were as follows ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast one-hybrid (Y1H) assays were performed according to the manufacturer’s instruction (Clontech, CA, USA). The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD ...
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bioRxiv - Systems Biology 2023Quote: ... The generated PCR products for the full-length proteins were transferred into the pMET7-GAG-sp1-RAS plasmid by classic cloning with restriction enzymes (EcoRI and XbaI) or In-Fusion seamless cloning (Takara Bio) when the genes contained internal restriction sites for EcoRI and XbaI (which was the case for CSDE1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.64 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... The RNA sample was then subjected to DNase I treatment (Takara #2270B) to remove DNA and purified again using ISOSPIN Cell & Tissue RNA Kit (Nippon Gene #314-08211) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... 12-well non-TC treated plates were coated with retronectin (Takara Bio T100B) at a concentration of 32ng/ml in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmids used in this study were constructed using In-Fusion® Cloning Technology (TaKaRa). Primers and genes were ordered from Integrated DNA Technologies (IDT) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.4 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... Mating was carried out in a 96-well plate according to manufacturer’s instructions (Matchmaker GAL4 Two Hybrid System (Clontech/TakaraBio). Mated diploid strains were serially diluted and plated on - Leu–Trp double dropout (DDO ...
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bioRxiv - Plant Biology 2023Quote: ... pYES2-SiHDA9 and empty pYES2 vector (EV) were transformed individually into Saccharomyces cerevisiae strain W303 using Yeastmaker Yeast Transformation System (Clontech). The transformed cells were screened on SD/-ura medium with 2% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... and treated with DNase I (Takara). Libraries for mRNA-seq were constructed using the KAPA Stranded RNA-seq Library Preparation Kit according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Digested DNA was ligated to adapters with T4 DNA ligase (Takara Bio Inc., Shiga, Japan) and purified with Agencourt AMPure XP cleanup reagent (Beckman Coulter ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech, TaKaRa Bio, USA). Serial dilution of transformed colonies were dropped on –Trp/-His synthetic dropout selection medium ...
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bioRxiv - Plant Biology 2023Quote: ... pGBKT7 and pGADT7 (Clontech), pCup-NuIGWY-Cyc1 (Wittke et al ...
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bioRxiv - Plant Biology 2023Quote: ... pGBKT7 and pGADT7 (Clontech, now Takara Bio, San Jose, CA, USA), as well as in pMETOYC-Dest (Xing et al ...
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bioRxiv - Synthetic Biology 2023Quote: Plant cDNA was prepared from 3 μg of each leaf RNA sample via RNA to cDNA EcoDry Premix (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of extracted RNA was subjected to one-step real-time RT-PCR using a One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.) on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Microbiology 2023Quote: ... We used Primer/Probe N2 (2019-nCoV) (TaKaRa Bio Inc.). The reaction conditions for RT-PCR were 42 °C for 5 min and 95 °C for 10 s ...
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bioRxiv - Plant Biology 2023Quote: ... pGBKT7 (Takara). Osr40C1 ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... the 1707 bp upstream sequence from the +1 site of the OsEG45 gene was cloned into the KpnI and SacI RE sites of the pAbAi vector (Takara). The OsGF14e gene was cloned into the pGADT7-Rec vector (Takara) ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNA library was prepared from rice flowers and ligated to the pGADT7-Rec vector using the Make Your Own “Mate and Plate” Library System (Takara). A second cDNA library was prepared from rice leaves under salt stress ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant plasmids were co-transformed into yeast Y2H Gold strain (Takara). The transformed yeast strains were grown on DDO (SD/-Leu/-Trp ...
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Bioengineering 2023Quote: ... 5.0 (TaKaRa). High-fidelity KOD-Plus-Neo DNA polymerase was used to amplify the sequences containing the degenerate codons ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids coding EGFP-mutants were generated from EGFP-IRES-mCherry plasmid using TaKaRa MutanBEST Kit (TaKaRa, Kusatsu, Japan). pG5egfp reporter vector was modified from the pG5luc vector (GeneBank Accession Number AF264724 ...
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bioRxiv - Neuroscience 2023Quote: ... were transiently cotransfected with a 2:1 mixture of Nav1.7-expressing plasmid8 and a pIRES2-ZsGreen bicistronic plasmid (Takara Bio, San Jose, CA) expressing ZsGreen and mouse FHF2B proteins.10 The same pIRES2-ZsGreen plasmid without FHF2 coding sequence served as the control ...
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bioRxiv - Molecular Biology 2023Quote: ... and reverse transcribed into cDNA using a PrimeScript RT Master Mix (Takara RR036A, Kusatsu, Shiga, Japan). Quantitative real-time RT-PCR analysis was performed using TB Green Premix Ex Taq (Clontech ...
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bioRxiv - Developmental Biology 2023Quote: ... and the cDNA was synthetised using the PrimeScript RT Master Mix Kit (TaKaRa, RR036A). The qPCR was performed using PerfectStartTM Green qPCR Super Mix (TransGen Biotech ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the cDNA was synthesized using PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). Real-time quantitative PCR was performed as previously described [13 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Neurons were transfected with GCaMP6 or NEMO-encoding plasmids at 7 to 9 days after plating using a calcium phosphate transfection kit (Takara Bio Inc).
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bioRxiv - Systems Biology 2023Quote: ... sSH2 domains were immediately purified by the N-terminal His6 tag with TALON® resin (Takara Bio) using a gravity column (detailed purification method in the Supporting Methods 1) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Stellar competent Escherichia coli cells (Takara Bio) were routinely grown in LB supplemented with kanamycin (Kan ...
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bioRxiv - Synthetic Biology 2023Quote: ... cloning was completed using InFusion (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... was prepared using In-Fusion Cloning system (Clontech, Mountain View, CA, USA). Two DNA fragments were independently amplified with two primer sets (NLS_Fw1/Rv1 ...
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bioRxiv - Synthetic Biology 2023Quote: Amplicons were prepared for sequencing using Takara ThruPLEX® DNA-Seq Kit (Takara Bio, cat #R400674), which included unique dual indexing ...
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bioRxiv - Systems Biology 2023Quote: ... 500 ng of isolated RNA was then used as input for generating cDNA and cDNA synthesis was performed using the PrimeScript RT kit (Takara Bio) according to the manufacturer’s instructions ...