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Citations for Takara Bio :
3301 - 3350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Lentivirus was harvested from the media at 72 hours concentrated by LentiX concentrator (Takara # 631232) per the protocol ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant lentivirus titer was measured using Lenti-XTM GoStixTM Plus (ClonTech, catalog number: 631280). E0771 and RM-1 cells were transduced with lentiviral vectors carrying the luciferase expression cassette with TransDux MAXTM (System Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was obtained from Addgene (Plasmid #71458) and cloned into pLVX-IRES-mCherry (Takara Bio).
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bioRxiv - Genetics 2023Quote: Total RNA from isogenic THP1 cells was extracted with the use of Trizol reagent (Takara, 9109), reverse-transcribed into cDNA with a PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The isolation of the upstream genome sequences of gnrh1 and gnrh3 of piranha was performed using the Universal GenomeWalker ™ kit (Takara Bio USA) according to the instructions ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was then used for library preparation using the SMARTer small RNA (smRNA)-seq kit for Illumina (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... here we inserted the two chaperones GroES and GroEL from the pGro7 plasmid (TaKaRa). The sequence for GroES and GroEL were amplified from the pGro7 plasmid by PCR and inserted into the linearized plasmid with NpnJ by In-Fusion® ...
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bioRxiv - Synthetic Biology 2023Quote: ... ∼¼ of a healthy uniform colony was suspended in complete synthetic medium (SCM) (Takara Bio USA). The cell concentration of each inoculum was measured by flow cytometry and then diluted to 1 cell/ul in an erlenmeyer flask ...
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bioRxiv - Synthetic Biology 2023Quote: ... The collected viral supernatant was concentrated using the Lenti-X concentrator (Takara Bio 631232) following manufacturing protocol.
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bioRxiv - Synthetic Biology 2023Quote: ... coated with RetroNectin (Takara Bio; T100B), where 3×105 activated T cells were seeded and then spun by centrifugation for 1000 g ...
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bioRxiv - Plant Biology 2023Quote: OsbZIP1.1 and OsbZIP1.2 CDS were cloned in pDEST-GBKTT7 (CD3-764) obtained from TAIR (https://www.arabidopsis. org) and transformed in the AH109 yeast strain (Clontech, TaKaRa Bio, USA). Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech, TaKaRa Bio, USA). Serial dilution of transformed colonies were dropped on –Trp/-His synthetic dropout selection medium ...
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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bioRxiv - Plant Biology 2023Quote: OsbZIP1.1 and OsbZIP1.2 CDS were cloned in pDEST-GBKTT7 (CD3-764) obtained from TAIR (https://www.arabidopsis. org) and transformed in the AH109 yeast strain (Clontech, TaKaRa Bio, USA). Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech ...
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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bioRxiv - Plant Biology 2023Quote: ... pGBKT7 and pGADT7 (Clontech, now Takara Bio, San Jose, CA, USA), as well as in pMETOYC-Dest (Xing et al ...
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bioRxiv - Plant Biology 2023Quote: ... using the In-Fusion system (Takara Bio, Shiga, Japan). The resultant MpFGMYB plasmid was digested with PmeI and NotI ...
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bioRxiv - Synthetic Biology 2023Quote: ... we concentrated harvested virus samples using a polyethylene glycol (PEG)-based method (65) using PEG 6000 (Wako #169-09125) or Lenti-X Concentrator (Takara #631231). When PEG 6000 was used ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Synthetic Biology 2023Quote: ... each for a 50-µL reaction containing ∼30 fmol of EcoRI-XbaI digested pLVSIN-CMV-Pur backbone plasmid (Takara #6183), ∼300 fmol of the insert fragment ...
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bioRxiv - Systems Biology 2023Quote: ... The total cfRNA library was prepared by SMARTer® Stranded Total RNA-Seq Kit – Pico (TaKaRa). Libraries were sequenced on Illumina HiSeq X-ten (~37.5 million paired-end reads per library ...
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bioRxiv - Systems Biology 2023Quote: ... Recombinant DNase I (TaKaRa) was used to digest DNAs ...
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bioRxiv - Systems Biology 2023Quote: ... The PBMC RNA library was prepared by SMARTer® Stranded Total RNA-Seq Kit – Pico (TaKaRa). All libraries were sequenced on Illumina HiSeq X-ten (~38.8 million per library ...
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bioRxiv - Plant Biology 2023Quote: ... with ROX as reporter (TakaRa). Three biological replicates were used for each of the above-mentioned sample type ...
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bioRxiv - Plant Biology 2023Quote: ... the promoter fragment was amplified and cloned between HindIII and XbaI sites using In-Fusion® HD Cloning Kit (Takara), generating pGWB502-HSP90.7pro ...
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bioRxiv - Plant Biology 2023Quote: ... T-DNA insertions were then analysed using specific primers (Table S1) in PCR reactions with Emerald Master Mix (Takara). PCR conditions were as follows ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast one-hybrid (Y1H) assays were performed according to the manufacturer’s instruction (Clontech, CA, USA). The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD ...
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bioRxiv - Systems Biology 2023Quote: ... The generated PCR products for the full-length proteins were transferred into the pMET7-GAG-sp1-RAS plasmid by classic cloning with restriction enzymes (EcoRI and XbaI) or In-Fusion seamless cloning (Takara Bio) when the genes contained internal restriction sites for EcoRI and XbaI (which was the case for CSDE1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.64 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... The RNA sample was then subjected to DNase I treatment (Takara #2270B) to remove DNA and purified again using ISOSPIN Cell & Tissue RNA Kit (Nippon Gene #314-08211) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... 12-well non-TC treated plates were coated with retronectin (Takara Bio T100B) at a concentration of 32ng/ml in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmids used in this study were constructed using In-Fusion® Cloning Technology (TaKaRa). Primers and genes were ordered from Integrated DNA Technologies (IDT) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.4 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... Mating was carried out in a 96-well plate according to manufacturer’s instructions (Matchmaker GAL4 Two Hybrid System (Clontech/TakaraBio). Mated diploid strains were serially diluted and plated on - Leu–Trp double dropout (DDO ...
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bioRxiv - Plant Biology 2023Quote: ... pYES2-SiHDA9 and empty pYES2 vector (EV) were transformed individually into Saccharomyces cerevisiae strain W303 using Yeastmaker Yeast Transformation System (Clontech). The transformed cells were screened on SD/-ura medium with 2% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... and treated with DNase I (Takara). Libraries for mRNA-seq were constructed using the KAPA Stranded RNA-seq Library Preparation Kit according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Digested DNA was ligated to adapters with T4 DNA ligase (Takara Bio Inc., Shiga, Japan) and purified with Agencourt AMPure XP cleanup reagent (Beckman Coulter ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech, TaKaRa Bio, USA). Serial dilution of transformed colonies were dropped on –Trp/-His synthetic dropout selection medium ...
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bioRxiv - Plant Biology 2023Quote: ... pGBKT7 and pGADT7 (Clontech), pCup-NuIGWY-Cyc1 (Wittke et al ...
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bioRxiv - Plant Biology 2023Quote: ... pGBKT7 and pGADT7 (Clontech, now Takara Bio, San Jose, CA, USA), as well as in pMETOYC-Dest (Xing et al ...
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bioRxiv - Synthetic Biology 2023Quote: Plant cDNA was prepared from 3 μg of each leaf RNA sample via RNA to cDNA EcoDry Premix (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of extracted RNA was subjected to one-step real-time RT-PCR using a One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.) on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Microbiology 2023Quote: ... We used Primer/Probe N2 (2019-nCoV) (TaKaRa Bio Inc.). The reaction conditions for RT-PCR were 42 °C for 5 min and 95 °C for 10 s ...