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Citations for Takara Bio :
3251 - 3300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 250 μg/mL proteinase K (Takara, Dalian), incubated at 55 °C for 1h ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted using RNAiso Plus reagent (Takara, Cat. 9108) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized from 500 ng of RNA using Primescript RT Reagent Kit (Takara) with random hexamer primers ...
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bioRxiv - Neuroscience 2023Quote: ... each pipette was filled with 3μl of pipette solution which consisted of 5% RNase inhibitor (Takara) in RNase-free PBS (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL of alkaline phosphatase (Takara, Okasa, Japan) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was incubated with 1 μL of S1 nuclease (Takara, Okasa, Japan) for 4 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... using the One-Step TB Green® PrimeScript™ RT-PCR Kit II (Takara) for RT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were ligated into pACT7_SC14 (HBB minigene reporter as previously described (31) using homology-based cloning technology (In-Fusion HD Cloning kit, Takara Bio). Following sequence verification ...
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bioRxiv - Cancer Biology 2023Quote: ... which was originally obtained from Clontech Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... The tissue was fixed for 30 min and the immunoprecipitation performed using a SEP3-specific antibody followed by library preparation using ThruPLEX DNA-Seq Kit (Takara) and deep sequencing65 ...
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bioRxiv - Cell Biology 2023Quote: ... and NIH/3T3 Tet-On 3G (Takara Bio, Kusatsu, Japan) cells were cultured in DMEM ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed by targeting the SARS-CoV-2 RdRp gene as follows: each 20 µl sample consisted of 12.5 µl One Step PrimeScript™ III RT-PCR Kit (Takara Bio, Shiga, Japan), 0.5 µM Sars-CoV-2 CRV forward primer (5’-TCACCTAATTTAGCATGGCCTCT-3’) ...
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bioRxiv - Microbiology 2023Quote: ... PolyA mRNA was used as input to SMARTer Stranded RNA-Seq Kit (Takara Biosystems) according to manufacturer’s instructions for library preparation ...
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bioRxiv - Immunology 2023Quote: ... mRNA was detected by RT-qPCR using SYBR TB Green Premix Ex Taq (Takara RR820W) and primers supplied by Eurofins Genomics or Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The collected supernatant was concentrated with LentiX concentrator following the manufacturer’s instructions (Takara 631232). The virus containing pellet was then resuspended in 1ml of the supernatant and stored in 100μl aliquots at -80°C.
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bioRxiv - Plant Biology 2023Quote: ... Phusion High-Fidelity DNA Polymerase (Thermo) or CloneAmp HiFi PCR Premix (Takara) was used to PCR amplify all the above sequences with primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was processed with the PrimerScript RT Reagent Kit (TaKaRa) according to the manufacturers’ instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... ATOM genes were cloned into the N1 vector (Clontech) under the CMV promoter using AgeI/NotI restriction sites ...
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bioRxiv - Cell Biology 2023Quote: ... Doxycycline is from Takara. Heparin solution is from Fresenius Kabi ...
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bioRxiv - Biochemistry 2023Quote: ... Tm1 FL for motility assays was cloned with a HisSUMO-SNAP-tag between SacI and HindIII sites of the pET11 vector by InFusion cloning (Takara). The SNAP-Tm1 1-335 truncation was made by site- directed mutagenesis of the SNAP-Tm1 FL plasmid using primers that inserted a stop codon after aa 335 as described above.
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bioRxiv - Biochemistry 2023Quote: ... the Tm1 coding sequence was inserted into MCS2 of pFastBacDual-HisSUMO- SNAP-3C-Khc between SmaI and KpnI sites by InFusion cloning (Takara). Tm1 FL for motility assays was cloned with a HisSUMO-SNAP-tag between SacI and HindIII sites of the pET11 vector by InFusion cloning (Takara) ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants from rescue plates were passaged on 15 cm plates of HEK 293T/17 cells (ATCC) transfected with pLV-TTBG and pLV-TTBL (described above) using Xfect transfection reagent (Takara 631318) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was further purified using mRNA Dynabeads and the purified mRNA was used as template for synthesis of cDNA using SMARTer PCR cDNA kit (Takara-Bio), with 18 cycles of amplification ...
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bioRxiv - Neuroscience 2023Quote: ... The relative titer of the lentiviral concentrate was estimated using Lenti-X GoStix (Takara).
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bioRxiv - Neuroscience 2023Quote: The Hes5.3-GFP plasmid derive from pEGFP-C1 plasmid (Clontech). The fragment bounded by the AseI and NheI restriction sites was deleted from pEGFP-C1 and the upstream sequences of Hes5.3 (1960 bp in length ...
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bioRxiv - Developmental Biology 2023Quote: ... and cDNA was synthesized using Primescript™ RT Master Mix (Takara, RR036B). The cDNAs were then used as templates for qRT-PCR analysis with gene-specific primers ...
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bioRxiv - Molecular Biology 2023Quote: CloneAmp™ HiFi PCR Premix (Takara) and 1 µl DMSO were mixed in a total volume of 25 µl H2O ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 1 ml Lenti-X Concentrator (Takara Bio; Cat. No. 631231), mixed thoroughly ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-Cherry (1:500; Clontech, 632543), Rabbit anti-Gat (1:4,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The SMARTer Stranded Total RNA-seq kit v2 - Pico Input (Takara) was used to prepare sequencing libraries ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracts were sent to Resistomap Oy (Helsinki, Finland) for HT-qPCR analysis using a SmartChip Real-time PCR system (TaKaRa Bio, Japan). The target genes included 27 ARGs and 5 MGEs (Table S3 ...
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bioRxiv - Microbiology 2023Quote: ... The protocol was as follows: PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (TaKara Bio, Japan), nuclease-free PCR-grade water ...
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bioRxiv - Biochemistry 2023Quote: ... TAKARA DNA polymerase (TAKARA) was used for PCR reactions ...
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bioRxiv - Biochemistry 2023Quote: ... Human embryonic kidney (HEK) 293T Lenti-X were obtained from Takara (cat# 632180). These cells transformed with adenovirus type 5 DNA and expresses the SV40 large T antigen ...
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bioRxiv - Bioengineering 2023Quote: ... with each 20 μl of PCR mixture containing 10 μl of TB Green Premix Ex Taq II (Tli RNase H Plus, Takara), 0.4 μl of each PCR forward and reverse primers (10 μM) ...
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bioRxiv - Cancer Biology 2023Quote: ... a region spanning the ENE domain was amplified by conventional PCR (PrimeStar HS mix, Takara Bio) with primer sequences listed in Table S9 ...
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bioRxiv - Plant Biology 2023Quote: ... The In-Fusion system (TaKaRa Bio) was employed to assemble the amplified sequences and green fluorescent protein (GFP ...
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bioRxiv - Biochemistry 2023Quote: ... or In-Fusion Cloning (Takara Bio Europe ...
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bioRxiv - Biochemistry 2023Quote: ... 2 Units of Exonuclease III (Takara) were added to 1ug of NCPs in ExoIII digestion buffer (50 mM Tris– HCl (pH 8.0) ...
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bioRxiv - Microbiology 2023Quote: ... or the BamHI/MluI site of pWPI-ACE2-zeo (for ACE2 expression plasmids)43 with 3×FLAG-tag at the C-terminus using In-Fusion® HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were amplified using PrimeSTAR GXL DNA polymerase (Takara Bio) under the following conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... The sequence spanning the I-SceI cleavage site in the SceGFP gene was amplified from 150 ng of genomic DNA using Advantage 2 Polymerase (Clontech) with primers dr-for (5’-CCCGCCACCTGCCCCATCTGCTA ...
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bioRxiv - Genomics 2023Quote: ... using calcium-phosphate (Takara). Media was changed at 24 hours and samples were collected 72 hours post-transfection.
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA was synthesized with the FastQuant RT Kit (Takara biomedical technology, Beijing, China) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... TRE3G promoter from pLVX-TRE3G plasmid purchased from Clontech was PCR amplified with Kpn1 in forward primer and Aar1 in reverse primer ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative real-time PCR was carried out using 2× TB-Green premix (TaKaRa) on a LightCycler-480®II (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... The gRNA was then ligated into BsaI linearized pKSE401 using DNA ligation Kit v.2.0 (TaKaRa, Kusatsu, Shiga, Japan).
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bioRxiv - Plant Biology 2023Quote: ... First-strand cDNA synthesis was performed using the Super Smart cDNA Synthesis kit (Takara, Japan). Gene-specific primers (Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... the cDNA encoding human Wt-syn was introduced into a vector from Clontech (Mountain View, CA) containing the mRFP sequence ...
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bioRxiv - Physiology 2023Quote: ... with 1.6 µg WT or mutant pcDNA5/FRT/TO Opti-hERG and 0.4 µg peGFP (Clontech, France) for fluorescence-based cell selection in microscopy24 ...