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Citations for Takara Bio :
3201 - 3250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR of mRNA was performed using TB Green Premix Extaq (Takara Bio) as per the manufacturer’s protocol on Quant Studio 12K Flex (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 1-2 μg RNA was reverse transcribed with random hexamers using PrimeScript 1st strand cDNA synthesis kit (Takara Bio, Japan) per the manufacturer’s protocol on Verti 96 well thermocycler (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: Gene encoding ARF-1 were amplified from cDNA (cDNA synthesized from RNA isolated from Hela cells) using high fidelity DNA polymerase enzyme from TaKaRa and cloned with N-terminal flag and HA tag in mammalian expression vector pcDNA3 flag HA Akt1 plasmid obtained from addgene(1477) ...
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bioRxiv - Cell Biology 2023Quote: SseI gene was amplified from Salmonella typhimurium (STM) strain LT-2 strain using high fidelity DNA polymerase enzyme from TaKaRa and cloned in with N-terminal flag and HA tag in mammalian expression vector pcDNA3 flag HA Akt1 plasmid obtained from addgene(1477) ...
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bioRxiv - Cell Biology 2023Quote: ... and used for library preparation (5-10 ng) using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Libraries were sequenced on Novaseq platform (Illumina).
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... cDNA generation and amplification were performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit for sequencing (Takara Bio USA, Inc.). Quality check was performed using High sensitivity D5000 ScreenTape® on TapeStation Analysis Software 3.2 (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... and ligation by In-Fusion cloning enzyme (Clontech).
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bioRxiv - Cell Biology 2023Quote: AAV plasmids were prepared by subcloning the ORF to pAAV-CAG-DNP63A7 or using the In-Fusion HD Cloning kit (Clontech).
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bioRxiv - Cell Biology 2023Quote: ... 2xmCh-KIF5C in pcDNA3.1 was cloned by inserting in-frame KIF5C full length from EGFP-KIF5C (a gift from Anthony Brown) 2xmCh sequence from KIF5C(1-560)-2xmCh-EF(C) into pcDNA3.1 using InFusion Cloning kit (Takara). EGFP-Rab7A (Addgene #28047 ...
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bioRxiv - Cell Biology 2023Quote: AAV8 was produced using AAV-Pro 293T cells (Takara #632273) cultured in one or more 15cm dishes ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-seq libraries were prepared with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara #634875). 75 bp single-end sequencing was performed using an Illumina NextSeq500 system at the CRI at UT Southwestern Sequencing Facility ...
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bioRxiv - Cell Biology 2023Quote: ... 30 cycles of 98 °C for 10 sec followed by 68 °C for 1 min using PrimeSTAR HS DNA Polymerase with GC buffer (Takara, R044A). The primers used for the purpose of inserting Capn4 into pCWX200 and pLexA were ...
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bioRxiv - Cell Biology 2023Quote: ... Glucagon (Takara, cat no. M182, 1:500), CCK (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... Human lung fibroblast MRC-5 cells were obtained from ATCC® (CCL-171™) and Lenti-X™ 293T cells were obtained from TakaRa (Mountain View CA, USA). All were propagated in complete DMEM (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: The recombinant Lenti-X™ pLVX-IRES lentiviral vector expression system (TakaRa) was used to introduce Wuhan-Hu-1 ...
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bioRxiv - Microbiology 2023Quote: ... Genes were inserted by homologous recombination using an In-Fusion HD cloning kit (Clontech) into an in-house modified pETDuet plasmid to yield a cleavable N-terminal His-SUMO fusion protein.
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bioRxiv - Microbiology 2023Quote: ... MMLV reverse transcriptase was purchased from Clontech, and PerfeCta SYBR Green SuperMix ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Clontech Laboratories) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... For STEM121 (1:5000, Takara, #Y40410) and Ki67 (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... FLuc_T2A_GLuc was cloned into MCS of pMSCV_hygro (Clontech Laboratories, Inc., 631461) using BglII (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... AmphoPack-293 cells (Takara Bio Inc., CVCL_WI47) were transfected with pMSCV-FLuc-T2A-GLuc-Hygro using calcium phosphate protocol69 ...
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bioRxiv - Cell Biology 2023Quote: ... The media containing lentivirus were collected from transfected cells and were further concentrated by 50-fold using Lenti-X Concentrator (Takara, #631231). RAB12 KO A549 cells were infected with lentivirus expressing WT 3XFLAG-RAB12 or 3XFLAG-RAB12 S106A mutant ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR analysis was performed with Light Cycler-480®II (Loche) using TB-Green (Takara, RR420) following the supplier’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... PrimeScript™ RT reagent kit (TaKaRa, RR036A) is used to generate cDNA from RNA extracted ...
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bioRxiv - Cell Biology 2023Quote: ... using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara, Kyoto, Japan). Preparation of PCR reactions was automated by the Echo 525 Acoustic Liquid Handler (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... and pAAVDJ using the In-Fusion HD Cloning kit (Clontech) and QuikChange II Site-Directed Mutagenesis Kit (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... The Bradford protein assay kit was purchased from Takara Bio (Shiga ...
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bioRxiv - Bioengineering 2023Quote: ... and transformed into Stellar Competent Cells (Takara). M13-F (GTAAAACGACGGCCAGTG ...
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bioRxiv - Bioengineering 2023Quote: ... and subcloned into pMSCV-neo (Clontech) using EcoRI and HpaI restriction sites ...
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bioRxiv - Bioengineering 2023Quote: ... HA-mEPOR was retrovirally transduced with pMSCVneo (Clontech) and selected with 0.6 U/ml human erythropoietin (Epoetin Alfa ...
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bioRxiv - Bioengineering 2023Quote: ... 2 µg pantropic pVSV-G (Clontech), 3 µg pCL- (Imgenex) ...
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bioRxiv - Bioengineering 2023Quote: ... via retroviral infection with the pRetroX-Tet-Off Advanced (Clontech) vector and selection with 1 mg/ml G418 ...
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bioRxiv - Bioengineering 2023Quote: ... CHAMP-1 or ErbB2TM cloned in the expression vector pRetroX-TIGHT-puro (Clontech) was introduced into cells expressing tTA by retroviral infection and selection with 1 µg/ml puromycin ...
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bioRxiv - Bioengineering 2023Quote: ... qRT-PCR was performed with TB Green Premix Ex Taq II (Takara RR820L). Each 20-μl reaction contained 2 μL of cDNA and 10 μL of TB Green solution with a final primer concentration of 200 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... the target mRNA CDS sequence was cloned using PrimeSTAR® Max (TAKARA, R045) or Q5® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... the fragments were subcloned into the pLVX-puro vector (Takara, #632164) with Ha- or Flag-tag ...
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bioRxiv - Cancer Biology 2023Quote: ... Wildtype and mutant constructs were then PCR amplified using primers to encompass HA-EZH2 + add NotI and PacI for subcloning into pQXCIH (Clontech). See Supplemental Table 1 for the primer sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... The adherent cells HEK293T-LentiX (TAKARA, Mountain View, CA) were grown in DMEM (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... First strand cDNA was synthesized using the Primescript RT Reagent Kit (TaKaRa) and 0.5 μg of total RNA in each cDNA synthesis reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA isolation and real-time PCR assay Total RNA was extacted by RNAiso Plus (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... coli competent cells (Takara Bio), from which single colonies were isolated from LB plates supplemented with ampicillin ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed according to the manufacturer’s protocol (TAKARA, Cat. No. RR047A) using 1 µL of the lysate as reverse transcriptase and TRIzol reagent-isolated 293T total RNA as template ...
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bioRxiv - Microbiology 2023Quote: ... CloneAmp HiFi polymerase (Takara) was used ...
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bioRxiv - Microbiology 2023Quote: ... 1µl (250U) PrimeStarGXL (TAKARA), and 5 μl of 10 μmol/μl “FWD 2 Deep seq” and “Rev Deep seq” primers (table S5) ...
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bioRxiv - Microbiology 2023Quote: ... 1µl (250U) PrimeSTAR GXL DNA polymerase (TAKARA), 4µl 2.5mM dNTP’s ...
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bioRxiv - Immunology 2023Quote: ... Transduction was performed on non-tissue culture 24-well plates previously coated with 0.5mL of RetroNectin (Takara) at a concentration of 25 μg/mL in PBS ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus) (Takara, RR420B) according to manufacturer’s instructions on an Applied biosystems StepOnePlus machine ...
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bioRxiv - Immunology 2023Quote: ... and 1 µg was used for cDNA synthesis with the PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). Of the cDNA ...
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bioRxiv - Microbiology 2023Quote: ... reverse transcription was performed by using the PrimeScript RT Kit (Takara Bio, San Jose, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription and tagmentation were conducted using the SMART-Seq v4 (Takara) and Nextera XT library prep kit (Illumina ...