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Citations for Takara Bio :
3151 - 3200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using the double primed RNA to cDNA EcoDry premix (Takara Bio). Primers were designed targeting FLuc ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed on a thermal cycler (TaKaRa) using TB Green Premix Ex TaqTM II (TaKaRa ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral titration was performed by RT-qPCR using the Lenti-X qRT-PCR Titration Kit (Takara) and the concentration of lentivirus used for this paper was found to be above 1 × 107 copies / mL ...
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bioRxiv - Cell Biology 2023Quote: ... Individual master mixes for each gene of interest were prepared using the TB Green® Premix Ex Taq II (TLi RNaseH Plus) reagent kit (RR820A, Takara Bio) and primer sets ordered from Sigma-Aldrich (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2023Quote: ... Entry clones for targeting and interaction assessment in mammalian cell culture lines were shuttled into the appropriate destination vectors including: pEGFP-N3 (Clontech), pmCherry-C1 (Clontech) ...
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bioRxiv - Bioengineering 2023Quote: ... GP2-293 cell line (Takara, CA) was used as the viral packing cell line for plasmid encoding CAR construct ...
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bioRxiv - Developmental Biology 2023Quote: ... Lenti-X cells were purchased from Takara and maintained in DMEM containing 10% FBS ...
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bioRxiv - Plant Biology 2023Quote: ... were PCR-amplified and fused to a nuclear localization signal (NLS) in pENTR vectors using In-Fusion HD Cloning Plus (Clontech). See Table S5 for primer details ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative real-time RT-PCR analysis was performed using TB Green Premix Ex Taq (Clontech, RR420A, Mountain View, CA, USA). The following diluted primers were used ...
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bioRxiv - Microbiology 2023Quote: Lenti-X 293T cells (Takara Bio Inc.) and its derivative ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was then removed using DNase I (Takara, Japan). CircRNA libraries were constructed using the TruSeqTM Stranded Total RNA Sample Preparation Kit from Illumina (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were added to the blocking solution (Rabbit anti-DsRed 1:1000, stock #632496, Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... dsRed anti-rabbit (1:500, Clontech, 632496), c- Fos anti-rabbit (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... with TB green premix Ex Taq (Takara, Japan). The primers used were listed in Table S1.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR amplification was typically conducted with ~20 overlapping DNA base primers and the PrimeSTAR HS DNA Polymerase (Clontech) or the Phusion Flash High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... pTRE3G-HA signal-Flag-GPCRs-P2A-mCherry-reverse-PGK-TetOn3G was made of Tet-ON 3G inducible expression system (Clontech) and PRESTO-Tango GPCR Kit (Kroeze et al. ...
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bioRxiv - Cell Biology 2023Quote: cDNAs of mouse Upf3a and Upf3b were amplified with PrimeSTAR® HS Premix (Takara) from an E14.1 ES cell cDNA library ...
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bioRxiv - Molecular Biology 2023Quote: ... All pHLP plasmids were created using the In-Fusion cloning system (Takara Biosciences) and were confirmed by Wide-seq analysis ...
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bioRxiv - Neuroscience 2023Quote: ... and mCherry (anti-dsRed polyclonal, 1:1000; Clontech, 632496). Sections were then washed in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was converted to double stranded cDNA and amplified using SMART-Seq v4 (Takara, Kusatsu, Shiga, Japan). Full length cDNA was quantified by 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... and then reverse transcribed into cDNA using PrimeScript II 1st strand cDNA synthesis kit (Takara Bio). PCRs for AgApiT and FNS I (Yan et al ...
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bioRxiv - Microbiology 2023Quote: ... the CPER products were transfected into HEK293-hACE2/hTMPRSS2 cells by using TransIT-LT1 (Takara, Cat# MIR2305). At one day post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... nine DNA fragments encoding the partial genome of SARS-CoV-2 (hCoV-19/Japan/TY-WK-521/2020) were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding the hepatitis delta virus ribozyme ...
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bioRxiv - Microbiology 2023Quote: A yeast screen for human cellular interacting proteins of pUL136 was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) and the Mate and Plate Universal Human Library (Clontech) ...
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bioRxiv - Microbiology 2023Quote: ... Yeast dual transformation and yeast mating was performed according to established protocols from Clontech. Yeast prey vectors were isolated from Y187 yeast and subsequently transformed into DH10B E ...
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bioRxiv - Molecular Biology 2023Quote: ... A Lenti-X™ GoStix (Takara) antigen test was used to verify successful lentivirus production.
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bioRxiv - Bioengineering 2023Quote: ... was cloned into the prey vector pPR3-N and co-transformed into yeast strain NMY51 cells together with pDHBⅠ-GhERF105a plasmid according to the Matchmaker user’s manual protocol (Clontech). Yeast transformants were selected by growth on synthetic dropout nutrient medium SD/-Leu/-His/-Ade/-Trp (Clontech ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Stellar™ competent cells (Takara) were combined with 5 µl reaction mix from the cloning step and incubated for 30 min on ice and subsequently heat shocked for 45 sec at 42 °C on a heating block ...
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bioRxiv - Neuroscience 2023Quote: ... pEGFP-C3 (Clontech), pCMV-tdTomato ...
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bioRxiv - Molecular Biology 2023Quote: Protein interactions in vivo in yeast were assayed using the ‘Matchmaker Gold Two-hybrid System’ (Clontech) using the protocols provided by the supplier ...
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bioRxiv - Systems Biology 2023Quote: ... cultures were spiked with 0.5 µM AP21967 (Takara, 635057) or 0.1% ethanol vehicle for 24 hours before cell lysis and biochemical analysis.
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bioRxiv - Systems Biology 2023Quote: ... cultures were spiked with 0.5 µM AP21967 (Takara, 635057) or 0.1% ethanol vehicle ...
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bioRxiv - Microbiology 2023Quote: ... and the Mate and Plate Universal Human Library (Clontech). This yeast two-hybrid universal library was constructed from human cDNA that has been normalized to remove high copy number cDNAs (overrepresented transcripts ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted using TaKaRa MiniBEST Plant RNA Extraction Kit (TaKaRa, 9769) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Plasmids for targeted gene deletion by homologous recombination were assembled using the In-Fusion® HD Cloning Kit (Takara Bio, Japan). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... obtained by respective modification of the pGBKT7 AND pGADT7 vectors (Clontech). Specifically ...
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bioRxiv - Neuroscience 2023Quote: ... with primers 5’-TCTTCCACTAGTGCCACCATGGCCACAGCTTGTAAAAGATC-3’ and 5’-TCTTCCGGATCCTTACAGCATGCCAAATCCTTTGG-3’ and subcloning into the SpeI and BamHI sites of the pLVX-IRES-mCherry vector (Clontech Cat#631237). HEK293T cells were transfected in 96-well using Lipofectamine 3000 Reagent (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Red Fluorescent Protein Antibody (DsRed, 1:500, Clontech). The brain sections were incubated with antibodies in blocking solution at 4°C temperature overnight ...
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bioRxiv - Neuroscience 2023Quote: ... differentiated HUES9 hESCs (Passage 12-18; RRID:CVCL_0057) were plated on 12-well iMatrix-511 (Cat.No. T303; Takara Bio Inc) coated plates at a density of approximately 3 x 106 cells/well in Synaptojuice medium (adapted from 124 ...
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bioRxiv - Neuroscience 2023Quote: The RNA isolation from the LVCP was performed with the NucleoSpin RNA plus XQ (Takara) following the manusfacturer’s protocol ...
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Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in TauopathybioRxiv - Neuroscience 2023Quote: ... and plasmids expressing EGFP (pEGFP-C1, Clontech) or Tau into SH-SY5Y cells (CRL-2266 ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR mixture containing 10 µL Emerald Master mix (2X) (TaKaRa), 2 µL total genomic DNA (10-15 ng/µL) ...
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bioRxiv - Molecular Biology 2023Quote: ... MaV108 was transformed with a DNA fragment that was amplified by PCR reaction using a plasmid pLexA (Clontech) as a template for HIS3 marker ...
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bioRxiv - Molecular Biology 2023Quote: HEK293XT cells (Takara Bio Lenti-X 293T, #632180) were a provided by the Yeo lab at UC San Diego (SEC-DIA versus SEC-TMT experiments) ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells and Lenti-X 293T cells (Takara, 632180) were cultured using a regular medium [Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: Lenti-X 293T cells (Takara Bio, 632180) were expanded until 80% confluency in a 3-layer 525 cm2 culture flask (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... Lentiviral supernatant was filtered through a 0.45 µm filter and concentrated using Lenti X Concentrator (Takara Bio, 631232) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and reverse transcribed into cDNA using PrimeScript RT Master Mix (Perfect Real time) (TaKaRa). cDNA copies of IBVs were quantified by real-time PCR using TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Microbiology 2023Quote: ... After transcribing using the In vitro Transcription T7 Kit (TaKaRa), BHK-21 cells were cotransfected with both full-length genomic and nucleoprotein gene RNA ...
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bioRxiv - Microbiology 2023Quote: ... In the In-Fusion PCR cloning system (Clontech), the S1 or S2 gene of the HV80 strain was replaced with the corresponding region of the H120 strain to construct the recombinant plasmids pMDVM8-HV80S1H120S2 and pMDVM8-H120S1HV80S2 ...