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Citations for Takara Bio :
2951 - 3000 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... TB Green Premix Ex Taq II (TAKARA-Bio) was used for PCReady primer-based assays ...
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bioRxiv - Physiology 2024Quote: ... Then Reverse transcription was performed using the PrimeScript RT reagent Kit (TAKARA-Bio, Shiga, Japan). Complementary DNA was subjected to quantitative RT-PCR to quantify mRNA expressions using PikoReal 96 and the PikoReal software (ThermoFisher) ...
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bioRxiv - Physiology 2024Quote: ... Proteins were extracted with RIPA lysis buffer supplemented with the protease inhibitor cocktail (Takara-Bio, Shiga, Japan). The lysate was sonicated using an ultrasonic homogenizer (Q55 ...
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bioRxiv - Physiology 2024Quote: ... and RNAiso Plus reagent (TAKARA). Complementary DNA (cDNA ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assays were based on the Matchmaker LexA two-hybrid system (Clontech). BPM genes were cloned into the pGlida vector ...
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bioRxiv - Plant Biology 2024Quote: ... YM4271 yeast strain was used in the Yeast-One-Hybrid screen (Matchmaker one-hybrid system, Clontech).
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bioRxiv - Physiology 2024Quote: ... and ZT17 of hawkmoth antennae with TB Green® Premix Ex Taq™ II (Tli RNase H Plus, Takara Bio Inc.). Primers for each target gene were designed using the Primer-BLAST online program of NCBI (Table 1) ...
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bioRxiv - Plant Biology 2024Quote: ... The dilutions were plated on selective -LT medium as a growth control and on -LTH medium (7.6 g/L Yeast Nitrogen Base [YNB], 20 g/L glucose, 0.62 g/L DO supplement -leu -trp -his [Clontech], 15 g/L agar) for the protein interaction assay ...
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bioRxiv - Plant Biology 2024Quote: ... The GFP antibody was purchased from Clontech (clone JL-8, 632380) and used at 1:2000 dilution ...
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized utilizing the PrimeScriptTM RT kit with gDNAEraser (Takara, Japan), following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... qRT-PCR assays were conducted in a 20 μL reaction volume employing TB Green Premix Ex TaqTM (Tli RNaseH Plus) (Takara, Japan). GAPDH served as the internal control ...
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bioRxiv - Plant Biology 2024Quote: ... and one of 15 µl purified DNA was used for real-time PCR amplification using SYBR Premix Ex Taq II (Tli RnaseH Plus) (TaKaRa) and Real-Time LightCycler 480 system (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 µg of total RNA was DNase treated and reverse transcribed using the PrimeScript RT reagent kit with gDNA eraser (Perfect real time) (TaKaRa) in a final volume of 20 µl and one microlitre of cDNA was used for subsequent amplification using SYBR Premix Ex Taq II (Tli RnaseH Plus ...
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bioRxiv - Plant Biology 2024Quote: ... in a final volume of 20 µl and one microlitre of cDNA was used for subsequent amplification using SYBR Premix Ex Taq II (Tli RnaseH Plus) (TaKaRa) and Real-Time LightCycler 480 system (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... were used for In-Fusion cloning with the In-Fusion Snap Assembly Master Mix (Takara), or for Gibson Assembly using the Gibson Assembly Master Mix (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... and the BsAUX/IAA16 amplicons were ligated into the digested vector using the In-Fusion Cloning kit (Takara Bio USA, Inc., San Jose, CA). Plasmid sequencing ensured correct assembly of expression vectors (Plasmidsaurus ...
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bioRxiv - Physiology 2024Quote: ... and the pCMV– EGFP–N3 plasmid was from Clontech, Inc.
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bioRxiv - Physiology 2024Quote: ... and 5 µg of total RNA was used for synthesizing complementary DNA (cDNA) using an RNA to cDNA EcoDry™ Premix Kit (Clontech, USA). Quantitative PCR (qPCR ...
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bioRxiv - Physiology 2024Quote: ... using TB Green® Premix Ex Taq™ (TAKARA, Japan). Quantitative PCR was conducted using a Quantstudio 1 Real-Time PCR instrument (Applied Biosystems) ...
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bioRxiv - Physiology 2024Quote: ... Homogenates were centrifugated at 1500 g for 10 minutes at 4°C before determining protein concentration by BCA assay (Takara Bio Inc.). Levels of 4-HNE-conjugated proteins were determined by Western blot ...
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bioRxiv - Physiology 2024Quote: ... The supernatants were used for the determination of antioxidant enzyme activities and protein concentration of supernatants measured by BCA assay (Takara Bio Inc.) for normalization.
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bioRxiv - Physiology 2024Quote: ... The concentration of AOPP was normalized to total protein content determined by bicinchoninic acid assay (BCA) assay (Takara Bio Inc.).
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bioRxiv - Neuroscience 2024Quote: ... Purification Kit (Takara) was used for viral particle purification ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... DNA encoding human Gαi/o/z subunits with the internal linker SGGGGTRSGTGGGGS containing restriction sites for KpnI and MluI were synthesized by Twist Bio and cloned into pCAGGS-SE at the KpnI site (site destroyed) by In Fusion cloning (Takara 638946). The insertion sites for each subtype can be found in Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and plant leaves using the Macherey-Nagel NucleoSpin Food Kit (Takara Bio Inc., Shiga, Japan).
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bioRxiv - Molecular Biology 2024Quote: ... using a TB green Premix ExTaq II (Tli RNaseH Plus) kit (Takara Bio Inc.) to label and amplify templates in accordance with the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... PCR was then performed using SYBR Green PCR Master Mix (Takara, Kyoto, Japan) for 40 cycles (95°C for 3 sec and 60°C for 30 sec) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The selected yeast strains were seeded in SD medium with Dropout (DO) supplement-His (Clontech, CA, US) on a sterile black clear-bottom 384-well plate (Corning ...
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bioRxiv - Physiology 2024Quote: ... WT females were deeply anesthetized with 0.02% MS222 and quickly fixed by perfusion with 4% paraformaldehyde (PFA) (Nakarai Tesque, Kyoto, Japan) in 1.0 × PBS (Takara Bio) for 30 seconds from the bulbus arteriosus using a borosilicate glass pipette made from capillaries with 1.5-mm outer diameters (GD-1.5 ...
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bioRxiv - Plant Biology 2024Quote: ... The extracted RNA was treated with Recombinant DNase I (TaKaRa Bio, Inc., Shiga, Japan) and cDNA was synthesized using the High-Capacity cDNA Reverse Transcription kit (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... with subsequent removal of genomic DNA by DNase I treatment (Takara). The extracted RNA was reverse transcribed into cDNA using the SuperScript VILO cDNA Synthesis Kit (Invitrogen Life Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... The detailed procedures for Y2H assays were conducted based on the manufacturer’s protocol (Clontech).
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bioRxiv - Plant Biology 2024Quote: ... vectors (Clontech). Different AD- and BD-derived vectors were co-transformed into yeast strain Y2HGold ...
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bioRxiv - Plant Biology 2024Quote: ... which was synthesized with the PrimeScript RT Master Mix (Perfect Real Time [Takara]) from RNA extracted with the RNeasy Plant Mini Kit [Qiagen] ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from leaves of maize seedlings using RNAiso reagent (Takara, http://www.takara.com.cn). First strand cDNA was synthesized using HiScript III RT SuperMix for qRCR (Vazyme ...
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bioRxiv - Plant Biology 2024Quote: ... The suspension was analyzed with a TaKaRa BCA Protein Assay Kit (T9300A, TaKaRa, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Using a Bio-rad Mx3000P qPCR system and SYBR Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa Biotech. Co.), qRT-PCR analyses were carried out ...
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bioRxiv - Molecular Biology 2024Quote: We cultivated HEK293T cells (Takara, #632180) in DMEM media (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: We generated a CRISPRi inducible HCT-116 RAD21-mAID-mClover cell line by transducing these cells with a plasmid expressing rtTA coupled to a neomycin resistance cassette (Takara, #631363). The cells were then put under selection by supplementing the media with 200μg/ml G418 Sulfate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The viral titer (p24 core antigen content) was determined initially using a LentiX GoStix Plus titration kit (#631280, Takara Bio, San Jose, CA, USA) and subsequently using an HIV p24 AlphaLISA detection kit (#AL291C ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100 ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were amplified using PrimeSATR GXL DNA Polymerase (TaKaRa, Ohtsu, Japan) from genomic DNA of IR64 and DJ123 ...
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Positive impact of Type-A and -C response regulator genes on growth and panicle architecture in ricebioRxiv - Plant Biology 2024Quote: ... and subjected to reverse transcription using a PrimeScript RT Reagent Kit (TaKaRa Bio). Quantitative reverse-transcription (qRT)-PCR was performed using a PRISM 7300 Real-Time PCR System (Applied Biosystems ...
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Positive impact of Type-A and -C response regulator genes on growth and panicle architecture in ricebioRxiv - Plant Biology 2024Quote: ... with SYBR® Premix Ex Taq™ (TaKaRa), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... according to the manufacturer’s instructions and reverse transcribed into cDNA using the PrimeScript RT reagent kit (Takara, Japan). SsoAdvanced Universal SYBR Green Supermix was used for qRT‒PCR (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... Human Fetal Brain Total RNA (Takara Bio USA, #636526) and Human Brain Total RNA (Ambion ...
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bioRxiv - Neuroscience 2024Quote: ... circularized using DNA Ligation Kit
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bioRxiv - Plant Biology 2024Quote: ... One or two gRNAs were inserted into the vectors using the BbsI site by ligation and/or In-Fusion HD Cloning (Takara). gRNA sequences are listed in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2024Quote: ... with SMART-Seq v4 Ultra Low Input RNA kit (Takara, Japan) for making cDNA library.
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bioRxiv - Neuroscience 2024Quote: ... Using an AAV titration kit (TaKaRa/Clontech), the viral vector titers were measured ...