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Citations for Takara Bio :
3001 - 3050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 10.5ul Clontech Lysis buffer (Takara Bio Inc. Cat # 635013). Lysed samples were stored at -80°C until transferred to the Duke Center for Genomic and Computational Biology Sequencing and Genomic Technologies core facility for cDNA library preparation and next- generation sequencing ...
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bioRxiv - Genetics 2023Quote: ... First-strand cDNA was synthesized using 1.5 µg RNA and Prime Script™ RT reagent Kit with gDNA eraser (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... double-stranded cDNA was synthesized and amplified using the SMARTer Universal Low Input RNA Kit protocol (Clontech, No. 634938), a product optimized for archived biosamples.29 The cDNA was sheared by Covaris ...
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bioRxiv - Developmental Biology 2023Quote: ... The constructed plasmids then were purified using nucleospin plasmid-transfection grade (TAKARA) and transfected into piezo1-deficient N2A cells (Sugisawa et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... about 50 ng synthesized template DNA was in vitro transcribed using in vitro Transcription T7 Kit (for siRNA Synthesis) (Takara bio), treated with DNase I (Takara Bio) ...
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bioRxiv - Developmental Biology 2023Quote: ... The converted DNA was amplified by PCR using Ex Taq Hot Start Version (Takara). And the PCR products were subcloned into pEASY-T5 Zero Cloning Kit (TransGen ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 μl of TB Green® Advantage® qPCR Premix (Takara, #639676), and 50 nM of each primer adjusted to 20 µl with water ...
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bioRxiv - Developmental Biology 2023Quote: ... The following antibodies were used: rabbit or rat anti-dsRed (1:200, Clontech 632496 or 5F8 1:400, Chromotek 5f8-100); chicken anti-GFP (1:800 ...
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bioRxiv - Developmental Biology 2023Quote: ... the oligo dT-based mRNA isolation kit (Takara Bio) was employed ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription reactions were conducted using the SMARTer RACE kit (Clontech 634859) or High-capacity cDNA Reverse Transcription kit (Applied Biosciences 4368814) ...
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bioRxiv - Developmental Biology 2023Quote: The dCas9-KRAB-NLS-NLS sequence was amplified from the plasmid PB-TRE-dCas9-KRAB-MeCP2 (Tan, 2022) (addgene 122267) using PrimeSTAR Max 2 ×premix (Takara). A T7 promoter sequence was included in forward primer for in vitro transcription using the mMESSAGE mMACHINE T7 Ultra Transcription kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... we confirmed successful repair by extracting mRNA and performing one step RT-PCR (Takara Primescript High Fidelity, RR057B). Primer sequences and design are detailed in Supplementary Fig 1 ...
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bioRxiv - Developmental Biology 2023Quote: The total RNA-seq libraries were constructed using the SMART-Seq Stranded Kit (Takara) as previously described (Wang et al. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli DH5α electrocompetent cells (Takara, Japan, product #9027), adding 2 uL of the product to 100 uL of electrocompetent cells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli BL21 (DE3) containing a pGro7 plasmid for overexpression of the GroEL/ES chaperone system (Takara Bio, Shiga, Japan). ACP (acyl phosphatase ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli BL21 (DE3) in the presence of GroEL/ES chaperones (pGro7 plasmid, TAKARA Bio, USA). After a pre-screen on agar plates ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded as described above and treated for 24 h with 500 nM Rapalog A/C hetero-dimerizer (Takara). Cells were collected as described above ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted in 50 mM NaCl at 100 ℃ for 30 min incubation followed by adjusting pH and PCR reaction using custom designed primers 23 and KOD Hot Start DNA Polymerase (Takara).
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bioRxiv - Cell Biology 2023Quote: The respective cDNAs encoding EGFP (Clontech, 6084-1), SNAP-tag (New England BioLabs ...
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bioRxiv - Cell Biology 2023Quote: ... Lenti-X cells (Clontech) were transfected with pMD2.G (addgene #12259) ...
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bioRxiv - Cell Biology 2023Quote: ... Contaminant DNA in 50 µg of extracted RNA was digested with DNaseI (cat. #2270A, Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Viral supernatants were collected and concentrated 20x using Retro-X concentraror (Takara Bio, PT5063-2), and stored at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... we used the pRetroX-Tet-on-advanced and pRetrox-Tight-Puro plasmids from Clontech Inc ...
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bioRxiv - Cell Biology 2023Quote: ... Clonal lines were genotyped by PCR using EmeraldAmp GT PCR Master Mix (Takara, RR310A) and target specific primers flanking the Myrlin transcription start site ...
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bioRxiv - Cell Biology 2023Quote: ... Y2H studies were based on the Yeast protocols handbook (Clontech, Protocol No ...
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bioRxiv - Cell Biology 2023Quote: ... 12.5 µL of Tks Gflex DNA polymerase Low DNA (cat. #R091A, Takara) and 12.2 µL of NFW} was added ...
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bioRxiv - Cell Biology 2023Quote: ... 1.6 µg of a transfer plasmid was transfected into LentiX 293T cells (Clontech Laboratories, inc.) with 0.8 µg of psPAX2 (addgene #12260 ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were generated using Gibson assembly and In-Fusion cloning according to manufacturer protocols (Takara, 638920). PB-NCT-GFP plasmids were generated using NCT plasmids donated by Zane Zeier and were flanked by PiggyBac transposon arms ...
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bioRxiv - Cell Biology 2023Quote: ... tdTomato (Takara Bio, 632496), Dystrophin (DSHB ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and mixed with Laemmli buffer after measuring the protein concentration using a TaKaRa BCA Protein Assay Kit (TaKaRa). Samples were subjected to SDS-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Cell Biology 2023Quote: Procollagen Type 1 C-peptide (PIP) was measured according to the manufacturer’s instructions (Takara, Shiga, Japan). For evaluation of PIP ...
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bioRxiv - Cell Biology 2023Quote: ... or In-Fusion cloning (Takara Bio). Full-length sequences of plasmids used in this study will become available at a public data share server upon publication.
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bioRxiv - Genomics 2023Quote: ... The same amount of input RNA for each batch was used for reverse transcription (RT) reactions with SMARTScribe™ Reverse Transcriptase (Takara) and oligo dT following the manufacturer’s manual ...
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bioRxiv - Microbiology 2023Quote: The plasmid constructs used as repair template for recombinant virus generation were generated using InFusion cloning (TaKaRa). The sequence references below are based on the HSV-1 KOS genome accession number JQ673480 (Macdonald et al 2012) ...
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bioRxiv - Genomics 2023Quote: LentiX HEK293T cells (Clontech, catalogue #632180) were maintained in DMEM high glucose with L-Glutamine and sodium bicarbonate ...
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bioRxiv - Immunology 2023Quote: ... Samples were processed using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Briefly ...
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bioRxiv - Immunology 2023Quote: ... ORF9c or ORF10 accessory proteins coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (Clontech, Takara, #631253) to generate pseudotyped lentiviral particles encoding each accessory protein of SARS-CoV-2 (Wuhan-Hu-1 isolate ...
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bioRxiv - Immunology 2023Quote: ... ORF9c or ORF10 accessory proteins coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (Clontech, Takara, #631253) to generate pseudotyped lentiviral particles encoding each accessory protein of SARS-CoV-2 (Wuhan-Hu-1 isolate ...
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bioRxiv - Immunology 2023Quote: ... and cloned into pLVX-IRES-Puro (Takara) via BamHI and BglII (V5-TurboID ...
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Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem functionbioRxiv - Microbiology 2023Quote: ... the 16S rRNA targeted oligonucleotide probes GAM42a (Table S1, TAKARA Bio Inc., Japan) was applied to the identification of γ-Proteobacteria ...
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bioRxiv - Microbiology 2023Quote: ... Site-directed mutagenesis was carried out using Prime STAR Max Premix as described in the manufacturer’s instructions (Takara Bio). All mutations were confirmed by DNA sequencing (Eurofins Genomics).
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Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem functionbioRxiv - Microbiology 2023Quote: ... Negative controls were performed by probe NONEUB (Table S1, TAKARA Bio Inc., Japan).
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bioRxiv - Genomics 2023Quote: ... cells were switched to media made with Tet System Approved FBS (Takara 631367) to reduce background induction ...
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bioRxiv - Genomics 2023Quote: ... and concentrated with Lenti-X concentrator (Takara) following the manufacturer’s manual ...
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bioRxiv - Genomics 2023Quote: ... Lenti-X 293T cells (Takara) are cultured with DMEM medium with 10% of FBS at 37°C and 5% CO2.
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was isolated from tail biopsies using the NucleoSpin Tissue kit (TaKaRa Bio USA, San Jose, CA) following manufacture protocol ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were performed with PrimSTAR GXL DNA polymerase (TaKaRa Bio USA) with 1 μl of genomic DNA and 0.2 μM primers ...
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bioRxiv - Genomics 2023Quote: ... The second PCR was performed using PrimeSTAR GXL polymerase (Takara) with 5 ng of purified product from the previous PCR was used as a template (50 μL/reaction) ...
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bioRxiv - Immunology 2023Quote: ... All fragments were synthesized as gBlocks (IDT) and subcloned into XhoI/NotI linearized SFG-4αβ by In-fusion cloning (Clontech). To co-express PD-1 with CARs ...