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Citations for Takara Bio :
2901 - 2950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The media was harvested 72 h post-transfection, filtered through a 0.45 μm filter (Millapore, Catalog #SLHVM33RS) and concentrated using LentiX (Takara Biosciences, Catalog #631232). Concentrated lentivirus was added to cells on day 2 of differentiation ...
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bioRxiv - Cell Biology 2023Quote: HaCaT cells were treated with the E-cadherin-blocking antibody (SHE78-7, Takara, Shiga, Japan), PY-60 (Axon Medchem ...
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bioRxiv - Cell Biology 2023Quote: ... First stand cDNA synthesis was performed using SmartScribe Reverse Transcriptase (Takara Bio USA #639538), and RNA was removed ...
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bioRxiv - Cell Biology 2023Quote: ... Using TB Green PreMix Ex Tap II (Takara) and specific primers ...
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bioRxiv - Cell Biology 2023Quote: ... Doxycycline is from Takara. Calcium chloride is from Acros Organics ...
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bioRxiv - Cell Biology 2023Quote: ... about ∼5 μg of bacmid were transfected using 5 μL of TransIT®-Insect transfection reagent (Takara Bio Inc.). 5 days after initial transfection ...
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bioRxiv - Cell Biology 2023Quote: ... concentrated with Lenti-X Concentrator (Clontech), and the pellet was dissolved in ADF medium (Thermo Fischer #12634) ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using PrimeScript™ RT Reagent Kit (Takara, Tokyo, Japan). qRT-PCR was performed on a Mx3000P real-time PCR system (Stratagene ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used for the study: mouse anti-GFP (632381, JL-8, Clontech, 1:1000), rabbit anti-mCherry (GTX128508 ...
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bioRxiv - Cell Biology 2023Quote: ... or pMSCV (Clontech). Drug resistance genes in lentiGuide and pLenti were replaced by blasticidin S ...
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bioRxiv - Cell Biology 2023Quote: ... or In-Fusion HD Cloning Plus (Takara 638909). Site-directed mutagenesis was performed by QuikChange Site-directed Mutagenesis Kit (Agilent 200523) ...
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bioRxiv - Cell Biology 2023Quote: ... doxycycline (0.03 µg/mL, 24 hours; TAKARA) and Biotin (500 µM ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNA fragments were amplified using PrimeSTAR HS DNA polymerase (TAKARA, Japan), and plasmid recombination was performed using NEBuilder HiFi DNA assembly (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... Expression plasmids encoding mCherry-tagged PDZ-RhoGEF and its mutants were constructed by inserting the PCR-amplified cDNA fragments into an mCherry-C1 expression plasmid (Clontech). The cDNA fragments were amplified using PrimeSTAR HS DNA polymerase (TAKARA ...
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bioRxiv - Cell Biology 2023Quote: ... The culture medium for SGE2 consisted of NDiff 227 (Y40002, Takara) supplemented with 1000 U/mL LIF (ESG1107 ...
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bioRxiv - Cell Biology 2023Quote: ... into ECFP-C1 expression plasmid (Clontech).
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bioRxiv - Cell Biology 2023Quote: ... The culture medium for rESCs consisted of NDiff 227 (Y40002, Takara) supplemented with 1000 U/mL LIF (ESG1107 ...
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bioRxiv - Cell Biology 2023Quote: ... 6-well plates of 70% confluent Lenti-X 293T cells (Clontech) were transfected with 1.5 μg transfer vector ...
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bioRxiv - Cell Biology 2023Quote: ... mouse MEFs (RIKEN BioResource Center, Tsukuba, Japan), mouse RAW264.7 macrophages (RIKEN BioResource Center, Tsukuba, Japan) and Lenti-X 293T (Clontech) were cultured at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using PrimeScript RT Master Mix (TAKARA). Real-time quantitative PCR was performed with CFX384 Touch (Bio Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Doxycycline (Dox, 631311) was purchased from Clontech. Lipids (egg SM and egg PC ...
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bioRxiv - Cell Biology 2023Quote: The lenti-CRISPR v2 constructs containing specific sgRNAs were transfected into Lenti-X 293T cells (Takara; 632180) with psPAX2 and VSV-G plasmids using the FuGENE HD transfection reagent (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... GSL-glycans were isolated by enzymatic digestion using EGCase I (Takara Bio Inc. Japan), whereas fOSs were recovered from an EGCase I-free fraction (46) ...
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bioRxiv - Cell Biology 2023Quote: ... and SYBR Premix Ex Taq II (Takara, Shiga, Japan) with gene-specific primers (S4 Table ...
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bioRxiv - Developmental Biology 2023Quote: ... LA Taq® high fidelity DNA polymerase (TaKaRa, Catalog #RR002A) was used to amplify the CRISPR-Cas9 target site according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and TaKaRa Thermal Cycler Dice (TaKaRa, Japan) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... Trizol reagent (200 μl; Takara Bio, Kusatsu, Shiga, Japan) was added to the 50 μl worm pellet and subjected to three freeze-thaw cycles in liquid nitrogen with intermittent vortexing for 1 minute to break open the worm bodies ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-DsRed (632496, Clontech), rabbit anti-Lcp1 (Jin et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... and reverse-transcribed into cDNA using Superscript RT Master Mix (Takara, Japan) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein concentration was quantified using the TaKaRa BCA Protein Assay Kit (TaKaRa, Japan), and 2-mercaptoethanol (Nacalai Tesque ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed using the TB Green Premix Ex Taq II (Takara Bio, San Jose, CA, USA) under the following cycling conditions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cDNA was synthesized using SMARTScribe Reverse Transcriptase (Takara, CAT# 639537) and PCR-amplified 2.5 mM DNTPs (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... using the In-Fusion ® HD Cloning Kit (Z9648N; TaKaRa).
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bioRxiv - Developmental Biology 2023Quote: ... Purified RNAs were then subjected to library generation using SMARTer Stranded Total RNA-Seq Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR was carried out using TB Green Premix Ex Taq II (Takara Bio) and monitored by 7500 Fast Real-Time PCR System ...
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bioRxiv - Evolutionary Biology 2023Quote: We prepared each sequencing library from 1 μg of total RNA using a PrepX PolyA mRNA Isolation Kit (Wafergen, 40047) on the Apollo 324 NGS Library Prep System (Takara Bio USA Holdings, Mountain View, CA). We added Illumina indices and amplified libraries with 12 cycles of PCR and cleaned them using PCRClean DX beads (Aline Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length human CLUH and mouse Cluh coding sequences cDNA were cloned in pcDNA3 and pmCherry-N1 (Clontech), respectively ...
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bioRxiv - Cell Biology 2023Quote: 1.0 μM of D/D solubilizer (Takara, Cat: # 635054) in Opti:MEM® (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Gal4-based Matchmaker Two-Hybrid System 3 (Clontech) was used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 13.32 (WT female) and 13.33 (WT male) were transduced with virus bearing either the empty pLVX-EF1a-IRES-ZsGreen1 vector (Takara), or the same plasmid expressing the coding sequence of C181 as described in lentivirus transduction ...
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bioRxiv - Cell Biology 2023Quote: ... Each PCR reaction was composed of CloneAmp HiFi PCR premix (Takara), 4ng of the plasmid template and 10-20ng of the primer pair mix ...
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bioRxiv - Cell Biology 2023Quote: ... an HR repair plasmid was constructed by amplifying homology arms 1000 bp upstream and downstream of the mTurquoise2 insertion site by PCR from genomic DNA obtained from murine CD1 keratinocytes and inserted using InFusion cloning (Takara) into the mTurquoise2-N1 plasmid (Addgene plasmid #54843;http://n2t.net/addgene:54843;RRID:Addgene_54843 ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA was collected with NucleoSpin RNA XS kit (Takara, 740902) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... from cDNAs generated with SMARTer Ultra low input RNA kit (Takara Bio) according to manufacturers’ instructions ...
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bioRxiv - Genetics 2023Quote: ... The PCR produc t was purified with a PCR purification kit (Takara Biomedical Technology, Bei jing, China) and was cloned into a pMDTM18-T vector (Takara Biomedical Tec hnology ...
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bioRxiv - Cell Biology 2023Quote: ... mEmerald/ mApple -VTA1: full-length human VTA1 was amplified by PCR and cloned to mEmerald or mApple -C1 vectors (Clontech). mCh-CHMP4C ...
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bioRxiv - Cell Biology 2023Quote: Plasmids NLAD8 HIV-1 AD8 Macrophage-Tropic R5 (NIH AIDS reagents #11346) or HIV Gag-imCherry V3 loop (29) were transfected in HEK 293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) in DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... PCR-mediated site-directed mutagenesis was performed with the pDsRed2-ER mammalian expression vector (Clontech). A BsiW1 restriction site was introduced by substituting adenine for guanine at the 1347th nucleotide (pDsRed2-ER-BsiW1mut ...
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bioRxiv - Genetics 2023Quote: ... The real-time PCR reaction system is as the followings: 10 µL of SYBR Premix Ex Taq™ (Takara Biom edical Technology, Beijing, China), 0.5 µM of each forward or reverses primer and 100-500 ng of DNA template ...
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bioRxiv - Genetics 2023Quote: ... real-time PCR was performed with SYBR Premix Ex Taq II (TaKaRa, China) on an iCycler RT‒PCR Detection System (Bio-Rad Laboratories ...