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Citations for Takara Bio :
2801 - 2850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and TBB green (#RR-208A, Takara) pre-mix Ex-Taq II (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2023Quote: ... these mutants were cloned into nmt1 promoter harboring pCST3 at BamHI site or pGADT7-AD (Clontech) at EcoRI site ...
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bioRxiv - Cell Biology 2023Quote: ... The virus titer was quantified using the AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... culture supernatant was recovered and mixed with a third volume of LentiX Concentrator (Takara Bio), incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... using TB Green Premix ExTaq II (Takara Bio) with the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... pT2K-EF1-rtTA-Neo was prepared using the In-Fusion HD cloning kit (Takara Bio) to recombine the rtTA/NeoR expression cassette excised by NotI/NheI from the PB-TAC-ERN and Tol2 backbone linearized by PCR ...
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from the total RNA of HapT1 cells using the PrimeScript RT Reagent Kit (Takara Bio). The amounts of Pts and Actb were quantified on a LightCycler 480 (Roche ...
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bioRxiv - Cell Biology 2023Quote: The full-length cDNA expression library was constructed using the SMARTer RACE 5’/3’ Kit (Takara Bio, 634858) according to the manufacturer’s instructions for the In-Fusion SMARTer Directional cDNA Library Construction Kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions for the In-Fusion SMARTer Directional cDNA Library Construction Kit (Takara Bio, 634933). 1µg of total RNA extracted from HapT1 with RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... The β-galactosidase activity was determined by ONPG (o-nitrophenyl β-D-galactopyranoside) liquid assay (quantitative) as mentioned elsewhere (Clontech Laboratories, 2008). β-galactosidase activity from each sample was calculated from at least three biological replicates and the concentration of cells (O.D.600 ...
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bioRxiv - Cell Biology 2023Quote: ... pLXIN (Clontech, 631501) containing the gene of interest and pCMV VSV-G at a ratio of 5:5:1 with polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... Restriction enzymes were purchased from TaKaRa Bio Inc ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA sequence of Bqt3 was amplified and cloned into pGBKT7-DB (Clontech) at EcoRI and BamHI sites for the yeast two-hybrid assay ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... supplemented with 12% FCS (Clontech), 100 U/ml penicillin (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... and Dusp27 genes were amplified using PrimeStar GXL DNA Polymerase (Takara Bio, Shiga, Japan) with soleus cDNA as the template ...
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bioRxiv - Cell Biology 2023Quote: ... the purified DNA fragment of the MyoD gene was cloned into a pAAV-CMV vector using the In-Fusion Cloning HD Kit (Takara Bio). The DNA fragment corresponding to the 300 bp 5′ upstream region (−250 to +50 ...
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bioRxiv - Cell Biology 2023Quote: ... DUSP13 deficient in phosphatase activity (=Dusp13_Mut) was generated using the PrimeSTAR® Mutagenesis Basal Kit (Takara Bio). The Dusp family contains the amino acid cysteine ...
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent proteins were probed with anti-GFP monoclonal (JL8, 1:2,000 dilution; TaKaRa Bio Inc.) and anti-RFP polyclonal (PM005 ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed with SYBR green master mix (Takara, Dalian, China) on the ABI Step One System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasma Gla-type osteocalcin was analyzed by enzyme immunoassay (#MK111, Takara, Japan). The absorbance was read at 450 nm using a microplate reader (Bio-Tek ...
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bioRxiv - Genomics 2023Quote: ... Eluted DNA was prepared as sequencing libraries with the ThruPLEX-FD Prep Kit (Takara bio, # R400675). Libraries were sequenced with 150-BP PE on an Illumina HiSeq 2500 Sequencing platform at Novogene.
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bioRxiv - Evolutionary Biology 2023Quote: ... and we subjected the stitched-amplicon product to NucleoSpin column cleanup (Takara). We transformed the stitched amplicon into Km31 by electroporation73 and selected transformants by plating onto agar plates with 200 μg/mL of G418 (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... EGFP and mCherry tagged cDNA constructs were cloned into pBMN and modified pLXIN (Clontech, Mountain View, CA, USA) retroviral expression vectors(Peden et al. ...
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bioRxiv - Cell Biology 2023Quote: Saccharomyces cerevisiae strain AH109 (Clontech) was maintained on yeast extract/peptone/dextrose-agar plates ...
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bioRxiv - Cell Biology 2023Quote: ... Gal4-activation domain (Gal4ad) vector pACT2 (Clontech) as described (Guo ...
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bioRxiv - Genomics 2023Quote: The nuclei containing about 1,000 ng of DNA were resuspended with 1x M buffer (Takara). To permeabilize nuclei ...
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bioRxiv - Genomics 2023Quote: The DNA was extracted from gills of a GinrinShowa and a Koganeryu using NucleoBond HMW DNA (Takara) following the manufactures’ protocol.
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bioRxiv - Genomics 2023Quote: ... The DNA was digested by 10 µl of Hind III (Takara) at 37℃ overnight in 250 µl of 1x M buffer solution ...
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bioRxiv - Genetics 2023Quote: ... High-quality total RNA was purchased from Takara (www.takarabio.com). Total RNA was isolated by a guanidinium thiocyanate method.27 Total RNA concentration ...
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bioRxiv - Genetics 2023Quote: Lenti-X HEK293T cells (Takara Bio, 632180) were maintained in DMEM/Ham’s F-12 supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Genetics 2023Quote: ... and lentiviral particles concentrated using Lenti-X Concentrator (Clontech, Mountain View, CA; catalog no. 631231).
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bioRxiv - Immunology 2023Quote: ... and used to generate Illumina-ready heavy and light chain sequencing libraries using the SMARTer Mouse BCR IgG H/K/L Profiling Kit (Takara, Cat# 634422). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Kit Advantage 2 PCR Enzyme System (Clontech, 639206), QIAquick Gel Extraction ...
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bioRxiv - Microbiology 2023Quote: ... H2AB2) was ligated in frame with the BglII and PstI restriction digest sites of pEGFP-C1 (Clontech). To construct the pEGFP-macroH2A1.2 expression plasmid ...
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bioRxiv - Microbiology 2023Quote: ... Amplified DNA encoding the sequence for H2A was ligated in frame with the BglII and SalI restriction digest sites of pEGFP-C1 (Clontech). To construct the pEGFP-H2A.X expression plasmid ...
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bioRxiv - Microbiology 2023Quote: ... Amplified DNA encoding the sequence for H2A.X was ligated in frame with pEGFP-C1 (Clontech) at the BglII and SalI restriction sites.
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bioRxiv - Microbiology 2023Quote: ... The macroH2A gene was ligated in frame with the BglII and PstI restriction sites of pEGFP-C1 (Clontech).
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bioRxiv - Microbiology 2023Quote: ... the pLVX-G9R+A1L-puromycin or pLVX-G9R+A1L-blasticidin plasmids were transfected into 293T cells using the Lenti-X Packaging Single Shots kit (631275, TaKaRa). Lentiviral supernatants were harvested 48 hours post-transfection and filtered through a 0.45 μm membrane (SLHV033RB ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from drug/vehicle-treated cell lines using NucleoSpin RNA kit (Takara, Catalog no. 740955.50). 2 ug of total RNA was used to synthesize cDNA with qScript Ultra Supermix (Quantabio ...
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bioRxiv - Immunology 2023Quote: ... Illumina libraries were constructed using SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (TaKaRa, Japan). RNA-seq libraries were sequenced at read lengths 2 × 151bp on the Illumina Novaseq 6000 (Illumina) ...
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bioRxiv - Immunology 2023Quote: miRNA was extracted from myotube-derived EVs using TRIzol reagent (Takara Biotechnology, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... PrimeScript RT Reagent Kit (Takara, RR037) was used for cDNA synthesis ...
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bioRxiv - Physiology 2023Quote: ... The SMARTer Stranded RNA Pico Mammalian V2 kit (Takara Bio) was used to create Illumina sequencing libraries ...
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bioRxiv - Neuroscience 2023Quote: Berghia brains (CRG plus rhg) were dissected and put into lysis buffer from the Smart-Seq4 kit (Takara Bio USA Inc., San Jose, CA) and stored at -20°C ...
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bioRxiv - Neuroscience 2023Quote: ... sagittal sections (50 µm) of the cerebellum were cut and incubated with antibodies against the reporter protein mCherry (Clontech 632543, RRID: AB_2307319, 1:500) and against the P-cell marker calbindin (Swant CB38 ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs coding for the potential interactors were cloned into pGBKT7 and pGADT7 vectors (Clontech) using different restriction sites ...
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bioRxiv - Plant Biology 2023Quote: ... or a custom cDNA library made from entire mature leaf material (Make Your Own “Mate & Plate” Library System, Clontech). Trimeric interactions (Y3H ...
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bioRxiv - Plant Biology 2023Quote: ... the Y2HGold strain was transformed with the bait vector (pGBKT7-SR45) and then mated with strain Y187 containing an Arabidopsis cDNA library (Mate and Plate Library-Universal Arabidopsis, Clontech) (Stankovic et al. ...
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bioRxiv - Immunology 2023Quote: ... The SMARTer Stranded RNA Pico Mammalian V2 kit (Takara Bio) was used to create Illumina sequencing libraries ...
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bioRxiv - Neuroscience 2023Quote: RNAs were extracted from cultured cells and tissues using RNA extraction kit and reverse transcribed into cDNAs (both from TAKARA) according manufacturers’ instructions ...