Labshake search
Citations for Takara Bio :
2751 - 2800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and 4.5 µl of recombinant ribonuclease (RNase) inhibitor (2313A,Takara Bio). The tissue was homogenized in tissue grinder (D9063 ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 ng of each RNA sample was reverse-transcribed with PrimeScript RT Master Mix (Perfect Real Time) (Takara Bio). qPCR was performed using the QuantiTect SYBR Green PCR Kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: ... the PrimeScriptTM RT Reagent Kit (Takara Bio Europe) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified using the NucleoSpin RNA Clean-up kit (Takara Bio). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... tubes with 1:1 FBS:PBS supplemented with recombinant RNase inhibitor (1:100, Takara). The live singlet gated CD3+ T cells were further gated as per the gating strategy shown in additional file 1 ...
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bioRxiv - Genetics 2023Quote: ... The Premix Taq™ (Takara Cat#R004A) was used for these PCR experiments.
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bioRxiv - Genomics 2023Quote: ... and extracted using the Nucleospin Gel and PCR Cleanup Kit (Takara, 740609) or Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... Digests were run on a 0.8% agarose-TAE gel and full-length plasmids were extracted with the Nucleospin PCR and Gel Cleanup Kit (Takara, 740609). A LR reaction was performed with 150 ng entry library and 150 ng pDEST_HC_Rec_Bxb_v2 ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed on 1 µg total mRNA per sample using PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... PrimeSTAR® Max DNA Polymerase (Takara Biomedical Technology (Beijing) Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... plasmids to Lenti-X HEK293T cells (Takara) to generate lentiviral particles for transduction of the target cells as described in detail previously (Elegheert et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed in triplicates using TB Premix Ex Taq II (Takara Bio, #RR390A, Japan) in a QuantStudio 6 Flex Real-Time PCR System using QuantStudio Real-Time PCR Software (both Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... and varying amounts (0, 10, 20, 100, 200 ng) of pAAV MyoD expression vectors into AAVpro 293T cells (Takara Bio) using the FuGENE HD transfection reagent (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... was then performed using a THUNDERBIRD SYBR qPCR system (TOYOBO) and a TP850 Thermal Cycler Dice Real Time System (Takara Bio, Shiga, Japan). The primer sequences are listed in Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... Infectious titers were measured using the Adeno-X Rapid Titer Kit (Takara Bio). MuSCs were cultured in a growth medium (GM ...
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bioRxiv - Cell Biology 2023Quote: ... and Rnase Inhibitor (1U/µl) (Clontech, 2313A) in 4µl final volume ...
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bioRxiv - Cell Biology 2023Quote: The yeast two-hybrid assay was performed according to the manufacturer’s protocol (Clontech MATCHMAKER System Manual, Clontech). Plasmids harboring Bqt4 or Bqt3 were co-transfected into Saccharomyces cerevisiae AH109 strain ...
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bioRxiv - Cell Biology 2023Quote: ... suspensions of the transformants (1.0×106 cells) were spotted onto SD plate media with dropout supplements,-Leu/-Trp or-Ade/-His/-Leu/-Trp (cat #630428) (Takara Bio), and incubated at 30°C for 3 days.
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bioRxiv - Cell Biology 2023Quote: The yeast two-hybrid assay was performed according to the manufacturer’s protocol (Clontech MATCHMAKER System Manual, Clontech). Plasmids harboring Bqt4 or Bqt3 were co-transfected into Saccharomyces cerevisiae AH109 strain ...
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bioRxiv - Cell Biology 2023Quote: ... and GFP-IDR-C (384-413 aa) were amplified via PCR and cloned into the pColdI vector (Clontech) at BamHI site ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells and hippocampal neurons were transfected with plasmids encoding FM4 recombinant receptors for 15 h and then treated with 2 μM DD-Solubilizer (TakaraBio/Clontech) to induce the release of the receptors from the ER into the secretory trafficking ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription of 500 ng purified total RNA was performed by PrimeScript RT reagent kit with gDNA Eraser (Takara, Dalian, China). RT-qPCR was performed with SYBR green master mix (Takara ...
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bioRxiv - Microbiology 2023Quote: ... reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adapter to the 3’ end of the cDNA by template switching using SMARTScribe Reverse Transcriptase (Takara ClonTech #639538) as described92 ...
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bioRxiv - Neuroscience 2023Quote: ... 45 cycles from 10 s at 95°C to 30s at 60°C using TB Green ® Premix Ex Taq™ GC (Takara Bio, Japan).
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bioRxiv - Neuroscience 2023Quote: ... AAV pro 293T (Takara Bio, Japan) cells were cultured in 150 mm dishes (VIOLAMO ...
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bioRxiv - Neuroscience 2023Quote: ... ESCs were seeded at 1 × 105 cells per well and maintained on the dishes at 37°C in 5% CO2 under humidified conditions with a 3i culture medium (Y40010, Takara Bio, Japan) without feeder cells ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA with mutations or deletion were constructed by site-directed mutagenesis using PrimeSTAR® HS DNA Polymerase mutagenesis kit (Takara Bio, Japan) following to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and the SYBR Premix Ex Taq GC (Takara Bio, Japan). Primers for qPCR (Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were then purified using TALON metal-affinity resin (Clontech, Mountain View, California, USA) previously added and washed in PBS according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... in a Mupid-One gel electrophoresis system (TaKaRa, Japan). RNA samples were purified for the second time by the TRIzol method as mentioned above and were stored at −80°C until further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... Cloning of the human SIK2 coding sequence to the pEGFP-C3 (Clontech, USA) vector was previously described by Küser-Abali et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... purified MBP-mEGFP/mCherry-Seb1 and MBP-mEGFP/mCherry-Rhn1 proteins were incubated with HRV 3C protease (Takara; 1 U/1 g of target recombinant proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged protein was eluted with 6ml glutathione (10mg/ml, Takara) and the flow-through was collected ...
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bioRxiv - Molecular Biology 2023Quote: ... gfp and rhn1 were prepared using an in vitro transcription T7 kit (Takara No.6140) and purified following the manufacturer’s instructions ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: Myristoylated TdTomato (myr::TdTom) was labeled with 1:500 rabbit anti-DsRed (Takara Cat# 632496, RRID:AB_10013483)
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bioRxiv - Neuroscience 2023Quote: ... AAV was titrated using quantitative PCR (qPCR) with primers for the ITR sequence (TaKaRa Bio Inc).
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bioRxiv - Neuroscience 2023Quote: ... HRV3C protease (TAKARA) was added overnight at 4°C and Ni-NTA was added to eliminate the cleaved portion overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids expressing HuSaV (GI.2) and PSaV protease-polymerase fusion were generated by cloning into the XhoI and BamHI sites of pmCherry-C1 (Clontech). Vectors expressing RIG-I-CARD domain [25] and IRF3-5D [26] were previously described ...
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bioRxiv - Microbiology 2023Quote: ... and contaminating RNA was degraded by using RNase A (TaKaRa Bio, Inc., Shiga, Japan).
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bioRxiv - Molecular Biology 2023Quote: ... Small RNA libraries were prepared using the SMARTer smRNA-seq Kit for Illumina (Takara, 635031) with 12 amplification cycles followed by bead size selection (ratio 0.95x-0.7x) ...
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bioRxiv - Neuroscience 2023Quote: ... the culture medium of the transfected cells was collected 72h post-transfection and mixed with LentiX Concentrator (Takara Biosciences: 631232) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and Takara NucleoSpin® RNA Clean-up(Takara Bio Inc, Kusatsu, Shiga, Japan). RNA integrity was confirmed by a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Real-time PCR was performed with SYBR RT-PCR kit (Takara, Dalian, China). The mRNA levels were normalized against β-Actin and presented as 2-ΔΔCt.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SYBR RT-PCR Kit was purchased from Takara (Dalian, China). PHrodo-labelled E ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Real-time PCR was performed with SYBR RT-PCR kit (Takara, Dalian, China). The mRNA levels were normalized against β-Actin and presented as 2-ΔΔCt ...
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bioRxiv - Developmental Biology 2023Quote: Single blastomeres or single blastocysts were lysed in 10X Lysis Buffer from Takara (cat.no. 635013), deposited in two Mosquito® plates ...
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bioRxiv - Developmental Biology 2023Quote: ... Only 0.5 µL of media containing cumulus cells from each mouse was added to 10 µL lysis buffer (Takara Bio Inc.). The oocytes were then treated with 1 mg/mL collagenase to remove the ZP ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted by using Sepasol®-RNA I Super G (nacalai, Tokyo, Japan) according to the manufacturer’s instructions and DNase-treated (Takara Bio Inc., Tokyo, Japan). cDNA was synthesized using SuperScript VILO (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Full-length cDNA was generated using a SMART-Seq HT Kit (Takara Bio) according to the manufacturer’s instructions ...