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Citations for Takara Bio :
2851 - 2900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... medium supplemented with 10% Fetal bovine serum (FBS, Clontech). One well from a 6-well-plate at 70% confluency was used for the transfection ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were purified via immobilized metal affinity chromatography (IMAC) with TALON metal affinity cobalt resin and its associated buffer set (Takara), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... in the presence of doxycycline (Clontech). pLenti CMV rtTA3 Hygro (w785-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 U/μl RNase inhibitor (Takara), 2 μM of Smart-seq2 template switching oligo (TSO ...
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bioRxiv - Cell Biology 2023Quote: ... pAcGFP1-Golgi from Clontech; mCherry-P4M-SidM (Addgene plasmid #51471),39 iRFP-FRB-Rab5 (Addgene plasmid #51612),39 and iRFP-FRB-Rab7 (Addgene plasmid #51613)39 from Dr ...
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bioRxiv - Bioengineering 2023Quote: ... Klenow Fragment (Takara Bio Inc, Japan) was then added to the mixture ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was purified from N2 using Nucleospin RNA®(Takara Bio Inc., Kusatsu, Japan) as described in manufacture’s procedure ...
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bioRxiv - Biochemistry 2023Quote: ... In-Fusion reactions were performed according to manufacturer’s guideline (Takara) using 100ng each of linearised plasmid and both inserts in 10μL ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked as above and then incubated overnight at 4 °C with appropriate antibodies to GFP (1:1000 dilution; Clontech, Catalog No. 1. 632381) and tubulin α (1:2000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Polymerase chain reaction (PCR) was performed using PrimeSTAR GXL DNA Polymerase (TaKaRa, R050A), following the recommended Rapid PCR protocol ...
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bioRxiv - Cancer Biology 2023Quote: We used the Lenti-X™ Tet-On® Advanced Inducible Expression System (Clontech, 632162) to produce the lentivirus vectors and transfect cells ...
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bioRxiv - Bioengineering 2023Quote: ... Purification and titer determination of the adenoviral particles were carried out using the Adeno-X™ Maxi Purification Kit (Takara Bio USA, San Jose, CA) and QuickTiter™ Adenovirus Titer ELISA Kit (Cell Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... The above lentivirus supernatant was then filtered through a 0.45 μm PVDF filter and concentrated using LentiX (Takara Biosciences). The final lentivirus can be added directly to the cells or stored in a -80 □ refrigerator.
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bioRxiv - Bioengineering 2023Quote: ... MirX (Takara) CDNA synthesis kits were used to detect miRNA expression ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was incubated with 1 ml NiNTA Superflow resin (Takara) overnight rotating at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Cell debris was filtered from the collected medium and Lenti-X Concentrator (Clontech) was then used to precipitate the lentivirus in the medium ...
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bioRxiv - Neuroscience 2023Quote: ... We used the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, 634894) to reverse transcribe poly(A ...
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bioRxiv - Synthetic Biology 2023Quote: ... The SEAP transgene from our previously developed plasmid pSurvivin-SEAP-WPRE (REF) was cloned into pHR_5x GAL4 UAS using In-Fusion HD cloning (Takara Bio, CA, USA) to make pHR_5x Gal4 UAS SEAP ...
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bioRxiv - Synthetic Biology 2023Quote: ... DNA amplification of phage or bacterial genetic sequences were performed by PCR using the CloneAmp HiFi PCR premix® (Takara©). Primers used in this study are listed in Table S1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were incubated at 3×104 cells/well in 96-well U bottom plates for 24 hours in T cell media containing 50 IU/mL rhIL-2 and varying concentrations of AP20187 (B/B homodimerizer, Takara, 635058). After 24 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... Stellar™ Competent Cells (Takara) were used for transformation ...
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bioRxiv - Molecular Biology 2023Quote: Lenti-X HEK293T cells (Takara Bio) were seeded at a density of 4.5×106 cells per 10 cm dish 18-24 h prior to transfection ...
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bioRxiv - Microbiology 2023Quote: ... the CPER products (25 μl) were transfected into VeroE6/TMPRSS2 cells using TransIT-X2 Dynamic Delivery System (Takara, Cat# MIR6003) according to our previous report22 ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.59) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 h posttransfection) ...
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bioRxiv - Molecular Biology 2023Quote: ... In-Fusion HD Cloning Kit (Clontech) was used for this reaction.
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... qTOWER3 G Real-Time System (Analytik Jena) Thermal Cycler Dice Real Time System III (Takara) or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Synthetic Biology 2023Quote: Polymerase chain reaction (PCR) amplification of DNA receivers for plasmids construction and colony PCR were performed using PrimeSTAR® Max DNA Polymerase (R045B, TaKaRa, Kyoto, Japan). PCR primers were synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Microbiology 2023Quote: ... using 3000 U of XbaI (Takara, Japan) following the PulseNet website protocols (https://pulsenetinternational.org/protocols/pfge/) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4x Lenti-X concentrator reagent (TaKaRa; 631231) was added to the lentivirus-containing supernatant at a final concentration of 1x and incubated at 4C for 1hr ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293T-LentiX cells (Takara Biosciences) were transfected with 750 ng of an equimolar mixture of the three third-generation packaging plasmids (pMD2.G ...
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bioRxiv - Synthetic Biology 2023Quote: ... for Infusion Cloning (Takara Bio). Plasmids were transformed into chemically competent NEB Turbo E ...
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bioRxiv - Synthetic Biology 2023Quote: Human Embryonic Kidney (HEK) 293 cells (ATCC, CRL-1573) and HEK293T-LentiX (Takara Biosciences) were cultured in standard culture conditions (37ºC and 5% CO2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Rapalog AP21967 (also known as A/C heterodimerizer, purchased from Takara Biosciences; catalog# 635056) was administered at the time of transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... A second 23-25 cycles PCR was performed with PrimeStar HS polymerase (Takara) or KAPA HiFi DNA Polymerase (Roche) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and pCMV-Tet3G Vector (Takara # 631335). Instead ...
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bioRxiv - Synthetic Biology 2023Quote: ... pTRE3G and rtTA3G sequences were amplified from pTRE3G-BI-ZsGreen1 (Takara #631339) and pCMV-Tet3G Vector (Takara # 631335) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PrimeSTAR HS DNA Polymerase was purchased from Takara Bio (Kusatsu ...
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bioRxiv - Synthetic Biology 2023Quote: A 10 cm dish of HEK293T cells that had been transfected with the GFP expressing plasmid pEGFP-N1 (Clontech) was washed with PBS and lysed by sonication on ice in PBS supplemented with 0.2 % Triton X100 ...
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bioRxiv - Cancer Biology 2023Quote: ... His-TEV tagged protein was captured with Co-TALON resin (Clonetech, Takara Bio USA, 2 mL slurry/liter culture) at 4 ºC for 1 h with constant end-to-end mixing ...
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bioRxiv - Synthetic Biology 2023Quote: ... SEAP activity in supernatant (25 μL) was measured using the Great EscAPe SEAP Chemiluminescence Assay kit 2.0 (Clontech, Fremonet, CA). The GLuc activity in supernatant (20 μL ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using the ThruPLEX DNA-Seq kit (Takara, R400675) and DNA Unique Dual Index Kit (Takara ...
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bioRxiv - Genetics 2023Quote: ... and DNA Unique Dual Index Kit (Takara, R400666). Paired-end sequencing of ChIP-seq libraries was performed (150 bp ...
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bioRxiv - Genetics 2023Quote: ... according to the manufacturer’s instructions (Clontech). Primers used for cloning and point mutagenesis are listed in Table S2.
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bioRxiv - Genetics 2023Quote: ... Amplicons were cloned into the NotI site of the pGL4.23 vector with pSox2 by In-Fusion® cloning (Takara Bio 639650). All plasmids were purified from bacterial culture using Presto™ Midi Plasmid Kit (Geneaid Biotech Inc ...
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bioRxiv - Genetics 2023Quote: ... All reactions were carried out to the manufacturer’s recommendations using Ex Taq polymerase (Takara Bio, Shiga, Japan) and primer pairs (Table S2 ...
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bioRxiv - Genetics 2023Quote: First-strand cDNA was synthesized from 5 μg of total RNA using the PrimeScriptTMII cDNA Synthesis kit from Takara (https://takara.com/). The full-length cDNAs of SUH1 and BC10 were amplified using the Taq LA DNA polymerase PCR kit from Takara (https://takara.com/ ...
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bioRxiv - Genetics 2023Quote: ... 200186 and purified by cobalt metal-affinity chromatography (Talon resin, Clontech 635652). cFos/cJun was stored in 18 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: Diploid human retinal pigment epithelium hTERT-RPE 1 cells (Clontech) were grown in DMEM/F-12 (Gibco ...