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Citations for Takara Bio :
51 - 100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 1000 ng of DNase-treated RNA was used in a 20 µL reaction employing oligo-dT primers and random hexamers according to the protocol provided by Takara (Cat# RR014B). The relative expression levels of the target genes were assessed via qPCR ...
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bioRxiv - Plant Biology 2024Quote: ... The JMJ27-GFP fusion protein was detected using the anti-GFP (Takara: 632593) 1:2,000 (v ...
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bioRxiv - Plant Biology 2024Quote: ... S1 and P1 fractions were loaded in denaturing buffer for SDS-PAGE separation and immunoblotted with the indicated antibodies (anti-DDB2: Molinier et al., 2008; anti-GFP: Takara-632593 ...
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bioRxiv - Microbiology 2024Quote: ... M-MLV reverse transcriptase (2641A) and TB Green® Fast qPCR Mix (RR430A) were purchased from Takara (Tokyo, Japan).
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bioRxiv - Microbiology 2024Quote: ... was amplified by ExTaq polymerase (Takara Bio) using the primers ...
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bioRxiv - Microbiology 2024Quote: ... Ex taq polymerase (Takara bio) was used according the manufacture’s instruction.
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bioRxiv - Genomics 2024Quote: ... First strand reverse transcription (RT) using Smartscribe Reverse Transcriptase (Clontech) and SmartSeq template switching oligo (TSO ...
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bioRxiv - Genomics 2024Quote: RNA was acquired from Takara (Cat# 636644). Multiplexing samples was done using one of two methods ...
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bioRxiv - Plant Biology 2024Quote: ... PrimeSTAR® GXL DNA Polymerase (Takara), or Prime STAR HS DNA Polymerase (TaKaRa ...
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bioRxiv - Plant Biology 2024Quote: Escherichia coli StellarTM Competent Cells (Clontech) was used as the host for DNA manipulation ...
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bioRxiv - Neuroscience 2024Quote: ... The viral titer was measured using a Lenti-X qRT‒PCR Titration Kit (#631235, TaKaRa, Shiga, Japan).
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (Clontech #632496, 1:500), chicken anti-GFP (Aves Lab #GFP-1020 ...
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bioRxiv - Neuroscience 2024Quote: ... SMART-Seq v4 ultra-low RNA (Takara Bio) library preparation protocol was used to generate cDNA libraries and sequencing was performed on an Illumina HiSeq 2 instrument with 150 bp reads according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: Single or multiple DNA fragments were cloned into binary vector using In-Fusion HD Cloning Kit (Clontech, USA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... and inserted into BamHI-digested pGEX-4T-3 by using In-Fusion Smart Assembly Cloning Kit (Takarabio-Clontech). Full-length OcKSL4 cDNA was amplified by RT-PCR using primers 5’-GGATCCATGGCGAATTATCCCATGGAG-3’ (forward ...
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bioRxiv - Neuroscience 2024Quote: ... Lentivirus-containing conditioned media were centrifuged at 500 x g for 5 min at 4°C to remove cellular debris and concentrated using Takara LentiX (Takara Cat# 631231). Briefly ...
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bioRxiv - Synthetic Biology 2024Quote: Cells were treated with Doxycycline (Clontech) and/or Shield1 (MedCehmExpress ...
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bioRxiv - Synthetic Biology 2024Quote: ... rapalog (Takara Bio, 635056), BioTracker 519 Green β-Gal Dye (Millipore Sigma ...
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bioRxiv - Systems Biology 2024Quote: ... Strand specific RNA-seq libraries were then constructed using the SMARTer Stranded RNA-seq kit (634485; Clontech, Kusatsu, Shiga, Japan), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and BFP/FAT-1/FAT-2 and T2A-NLS-mApple fragments were inserted with InFusion cloning (Takara Bio), as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... after which Lenti-X Concentrator (Takara Bio) was added at a 1:3 ratio ...
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bioRxiv - Neuroscience 2024Quote: ... sandwiched in between 300 bp each of repeat-adjacent upstream and downstream sequence from C9orf72 intron 1 and then inserted it into the internal MCS of pCDH-EF1-MCS-IRES-copGFP with InFusion cloning (Takara Bio) in between XbaI and NotI restriction sites ...
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bioRxiv - Synthetic Biology 2024Quote: ... The inducible ABE plasmid was made by integrating a tet-responsive promoter (TRE3G, Takara Bio) and ABE 7.1050 (Addgene #102919 ...
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T cells (Takara Bio #632180) were seeded at approximately 7e5 cells/well in a 6-well plate to yield ∼80% confluency the following day ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... and 20x concentrated using LentiX Concentrator (Takara) according to the manufacturer’s instructions ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 1x Titanium Taq buffer and 2 μL Titanium Taq polymerase (Takara) were mixed in 50 µL total volume ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 1x Titanium Taq buffer and 2 μL Titanium Taq (Takara). PCR cycles were ...
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bioRxiv - Systems Biology 2024Quote: ... Lenti-X 293T (Takara), and PlatinumE (Cell Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: Knockdown of endogenous gene expression was performed using the RNAi-Ready pSIREN-RetroQ Vector (Clontech). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Equal amounts of total RNA were used to synthesize cDNA with the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara RR047A, China). Quantitative real-time reverse-transcription PCR (qRT–PCR ...
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bioRxiv - Immunology 2024Quote: ... RNA was reverse transcribed into cDNA using a PrimeScript RT Reagent Kit (Takara, Japan). qPCR was performed using QuantStudio™ Test Development Software (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... using the SYBR Premix Ex TaqTM (TaKaRa, Japan). Gene expression was normalized using UBQ10 and ACT2 as internal standards ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid analysis was performed using the MatchMaker GAL4 Two-Hybrid System 3 (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The cell lysate was then clarified with centrifugation and the supernatant was collected and loaded onto a gravity column manually loaded with TALON Metal Affinity Resin (Takara), washed 5 times with 10 column volumes (CV ...
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bioRxiv - Biophysics 2024Quote: ... Lentivirus was concentrated 15-fold using LentiX concentrator reagent (Takara), resuspended in PBS ...
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bioRxiv - Microbiology 2024Quote: ... and assembled by In-Fusion (TaKaRa, Japan). After purification with the NucleoSpin Plasmid kit (Macherey Nagel ...
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bioRxiv - Immunology 2024Quote: ... RNAse Inhibitor (Takara)) in a 96-well V-bottom PCR plate (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... DNA adapters were ligated to MmeI-digested DNA fragments by T4 DNA ligase (Takara), and the ligation products were purified on magnetic beads ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara, RR820B) on a Real-Time PCR detection system (BioRad) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed into complementary DNA (cDNA) using PrimeScript™ RT Reagent Kit (Takara, RR047B). Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... and cDNA was synthesized using the PrimeScript RT reagent kit with gDNA eraser (TaKaRa). A random hexamer was used for cDNA synthesis ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized at 44°C for 15 min using the PrimeScript RT reagent kit with gDNA eraser (TaKaRa) and the y300 PAT universal C10 primer ...
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bioRxiv - Molecular Biology 2024Quote: ... qRT–PCR was performed using SYBR Premix Ex TaqII (Tli RNaseH Plus) and the Thermal Cycler Dice Real Time System (TaKaRa). The data were normalized using 18S rRNA as a reference ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... Lenti-X 293T cells (Takara) were cultured in complete DMEM supplemented with 10% fetal bovine serum and 1% antibiotics (pen/strep).
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: Lenti-X 293T cells (Takara) were seeded at 65,000 cells per cm2 in 10 mL media (DMEM ...
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bioRxiv - Synthetic Biology 2024Quote: ... The human EF1α promoter was sourced from pLVX-Tet3G (Clontech/Takara). Barcodes used for the TUPVs were designed by the Elledge lab34 ...
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bioRxiv - Systems Biology 2024Quote: ... and titers were verified by Lenti-X GoStix Plus (Takara Bio 631280) before storage at -80°C.
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bioRxiv - Systems Biology 2024Quote: ... We used 600 µl of cell lysate for total RNA extraction (Clontech 740955), followed by processing for microarray (for interactome assembly ...
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bioRxiv - Synthetic Biology 2024Quote: ... and spectinomycin-resistant gene with lacI–spac promoter unit were amplified through polymerase chain reaction (PCR) using PrimeSTAR DNA polymerase (TaKaRa, Shiga, Japan), along with appropriate primer sets (namely F1/R2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Gal4-NGN2 plasmid was constructed using Gibson Assembly with In-Fusion® Snap Assembly Master Mix (Takara Bio #638947) and following recommended manufacturer’s instructions ...