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Citations for Takara Bio :
151 - 200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was incubated with 35μl of Talon metal affinity resin (TaKaRa) at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Lenti-X 293T (Cat# 632180, Takara Bio) cells were seeded on 6-well plates (∼400,000 cells/well ...
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bioRxiv - Biochemistry 2024Quote: ... GFP (mouse, Clontech cat# 632380; 1:2,000); FLAG (mouse ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... cDNA was generated using SMART-seq v4 Ultra Low Input RNA Kit (Takara Bio) and the final sequencing library was made using the Nextera XT Kit (Illumina ...
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bioRxiv - Bioengineering 2024Quote: ... SEAP production was analyzed by collecting medium and running a chemiluminescent assay on samples (Takara Bio) using a Tecan Infinite M1000 Pro plate reader.
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bioRxiv - Bioengineering 2024Quote: cDNA and the plasmid library were amplifed using CloneAmp PCR Master Mix (Takara 639298) and primers for amplicon sequencing (Supplemtary Note 23) ...
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bioRxiv - Bioengineering 2024Quote: ... and the receptor- or payload-encoding viral expression plasmids (2.0 μg) into Lx293T cells (Clontech) in 6-well plates with Lipofectamine 3000 (Thermo Fisher L3000001) ...
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bioRxiv - Bioengineering 2024Quote: Primers oAS344 and oAS345 were then both used to amplify the cDNA using CloneAmp PCR Master Mix (Takara 639298) according to manufacturing protocol ...
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bioRxiv - Bioengineering 2024Quote: TB Green Advantage qPCR premix (Takara Bio, Cat. No. 639676) was used for the amplification of osteogenic and chondrogenic markers ...
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bioRxiv - Bioengineering 2024Quote: ... All proteins were purified with a Talon metal affinity resin (Takara Bio USA, Mountain View, CA, USA) and dialyzed against pyrogen-free PBS ...
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bioRxiv - Bioengineering 2024Quote: ... was cotransfected into HEK293T cells (Takara) with the packaging plasmid psPAX2 (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... was subcloned into the lentiviral expression vector pLVX-M-puro (Takara 632164). For CRISPR-KO experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Pairs of pGBKT7 and pGADT7 plasmid were co-transformed into the yeast strain AH109 following the MatchmakerTM GAL4 Two-Hybrid System instructions (Clontech). Primary transformants were selected on synthetic drop-out (SD ...
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bioRxiv - Cancer Biology 2024Quote: ... the total RNA of fibroblasts was isolated with RNAiso reagent (Takara Bio, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... lentivirus targeting mCherry to the cell membrane (Takara, 0026VCT, rLV.EF1.mCherry-Mem-9) was used according to manufactures recommendation ...
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bioRxiv - Bioengineering 2024Quote: ... Appropriate colonies were grown and the recombinant bacmid DNA was extracted using the NucleoBond Xtra Midi plasmid purification kit (TAKARA). The fifth instar silkworm larvae ...
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bioRxiv - Bioengineering 2024Quote: AAV-vector plasmid and AAV-crTSG were used for AAV9 production and purification using Lenti-X 293 T cells (Takara Bio). Each set of Lenti-X 293 T cells (6 150mm-plates ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid pmCherry was generated by fusing the lacI-Ptrc inducible system and mCherry into the vector pBBR1MCS-5 using In-Fusion® Snap Assembly Master Mix (Takara Bio). All plasmids were extracted using QIAprep® Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Bioengineering 2024Quote: ... and reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio). The resulting cDNA was utilized for analysis using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase 8 reporter was established by replacing the DEVD amino acid sequence with XEXD in pLenti_CMV_GFP_Blast_FLIP_GFP_DEVD plasmid through the In-fusion Snap assembly master mix (Takara Bio) using the following oligos forward ...
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bioRxiv - Bioengineering 2024Quote: ... mixed with one-tenth of the volume of Lenti-X Concentrator (Takara Bio), and incubated at 4 °C for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... The cleared supernatant was then mixed with Lenti-X Concentrator (Takara Bio) and incubated for 24 h at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... Viral genome number was quantified by real-time PCR with AAVpro Titration Kit Ver.2 (Takara, 6233) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... AAV was purified with the AAVpro Purification Kit (Takara, 6666) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... filtered with a 0.45μm filter to remove cellular debris and concentrated (100x) using Lenti-X concentrator (Takara, San Jose, CA). The resulting particles were resuspended in DMEM with 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were constructed using the SMART-Seq® Stranded Kit (catalog no. 634442, TaKaRa) and indexed using the SMARTer® RNA Unique Dual Index Kit (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... From cortical neurons subjected to OGD we generated 1) library to detect circularRNAs and mRNA and lncRNAs using SMARTer® Stranded Total RNA Sample Prep Kit (Takara Bio USA, Inc.) and 2 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cDNA synthesis and gDNA erase protocol were performed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara). Quantitative real-time RT-PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse-transcribed using a cDNA synthesis kit (TaKaRa Bio, Kusatsu, Japan). RT-qPCR was conducted using SYBR Green PCR Master Mix (TaKaRa ...
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bioRxiv - Cancer Biology 2024Quote: ... and the remaining RNA isolation was done using the NucleoSpin RNA II Kit (Clontech Laboratories, Palo Alto, CA; 740955.50). 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... we employed the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) or WST-1 (Water-Soluble Tetrazolium 1) assay from Takara, Japan ...
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bioRxiv - Cancer Biology 2024Quote: 1 x 106 ACKP cells were transfected with 3 µg pE2F-TA-luc plasmid (Takara) and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... One to six nanograms of RNA were used to generate a cDNA library using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara). Samples were sequenced with a NextSeq 500 (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR were performed using PrimeSTAR Max DNA Polymerase (Takara, ref R045A). 0.1pmol of each fragment were then assembled for 1h at 50°C using NEBuilder HiFi DNA Assembly master mix from the NEBuilder HiFi DNA Assembly Cloning Kit (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The tetrazolium salts from Premix WST-1 Cell Proliferation Assay System (TAKARA) are cleaved by mitochondrial dehydrogenase in viable cells to produce formazan dye ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested and vectors were purified using the AAVpro purification kit (cat.: 6666; Takara Bio, Kusatsu, Shiga, Japan) as per manufacturer’s instructions and stored at -80 °C until further use ...
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bioRxiv - Genetics 2024Quote: ... Taq (Takara) or Q5 (NEB) ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed as standard procedures in 10 μl reactions by TaKaRa Ex Taq ...
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bioRxiv - Microbiology 2024Quote: ... Each reaction was performed in a 25-μl volume containing 12.5 μl SYBR Premix Ex Taq (TaKaRa Biotechnology, Otsu, Shiga, Japan), 0.5 μl of forward and reverse primers (10 μM) ...
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bioRxiv - Genetics 2024Quote: ... PCR amplifications were done in duplicates using the PrimerSTAR GXL polymerase (Takara Bio) and 250 ng of genomic DNA per reaction ...
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bioRxiv - Genetics 2024Quote: ... Real-time PCR was carried out using SYBR Premix Ex TaqTM II (Takara) on the Applied Biosystems StepOne Real-Time PCR System ...
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bioRxiv - Genetics 2024Quote: ... then they were sequenced directly or cloned into the pMD18-T Vector (TaKaRa) for sequencing.
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bioRxiv - Genetics 2024Quote: ... PCR was performed as standard procedures in 50 μl reactions by TaKaRa LA Taq ...
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bioRxiv - Developmental Biology 2024Quote: ... Two targeting plasmids were constructed with InFusion cloning (Takara Bio), one consisting of 5’ and 3’ homology arms (each 1kb in length) ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were stained with anti-mouse E-cadherin monoclonal antibody ECCD-2 (1:100, Takara) for 16 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: RNA extraction was carried out using RNAiso Plus (TaKaRa, Dalian, China) following the manufacturer’s instructions with some modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... the larvae were embedded in agarose gel (5805A, Takara) in E3 buffer supplemented with anesthesia and N-phenylthiourea and observed using a TE2000 microscope (Nikon ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we made a master mix using the primers 2550F = 5’-GTT ACT GAT TCG TCT ACG AGA-3’ and 2718R = 5’-ATT GAA ATG ATC CAG TGC TTG-3’ and TaKaRa ExTaq DNA Polymerase (Takara Bio USA, Inc). We ran the samples for 31 cycles and ran the PCR product on a 2% agarose gel.
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bioRxiv - Evolutionary Biology 2024Quote: ... rapid amplification of cDNA ends (RACE) was performed using the SMART RACE cDNA Amplification Kit (Clontech). For LhGAP3 ...
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bioRxiv - Molecular Biology 2024Quote: qPCR was performed using TB Green Fast qPCR Mix (catalog no. RR430A, TaKaRa) according to the manufacturer’s instructions ...