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Citations for Takara Bio :
101 - 150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T cells (Takara Bio #632180) were seeded at approximately 7e5 cells/well in a 6-well plate to yield ∼80% confluency the following day ...
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T packaging cells (Clontech #11131D) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2024Quote: ... Polymerase chain reaction (PCR) was performed using Primer STAR Max DNA Polymerase (Takara) to amplify the target gene ...
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bioRxiv - Neuroscience 2024Quote: ... The preserved HEK medium was then spun down with 12.5 ml of lenti-X concentrator (Takara Bio), in an effort to capture the two distinct lentiviruses ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries of cDNA were then generated using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara). The resulting cDNA libraries were then quantified by the High Sensitivity DNA assay ...
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bioRxiv - Neuroscience 2024Quote: ... and re-plated at a concentration of 2.5×105 cells/cm2 onto plates coated with 4 μg/mL iMatrix-511 (Takara Bio) in N2B27 medium supplemented with 10 μg/mL BDNF and 10 µg/mL Rock Inhibitor Y-27632 ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit Anti-dsRed (Takara 632496, 1:1000); Goat Anti-Calb2 (Swant CG1 ...
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bioRxiv - Molecular Biology 2024Quote: ... After RNA Reverse transcription (Prime Script RT reagent, Takara), cDNA levels were measured by qPCR Fast SYBR™ Green Master Mix (Ref ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti-dsRed (Takara 632496, 1:1,000–1:2,000), and Chicken anti-GFP (Aves GFP-1020 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA synthesis was performed using a one-step PrimeScript RT-PCR kit (Takara Bio, Cat. RR057B, Japan) as per the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... using a random primer DNA labeling kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... a two-round PCR strategy was required to amplify the ORF using a high-fidelity PrimeStar ® DNA polymerase (Takara Europe, Saint-Germain-en-Laye, France). The first round of the nested PCR was carried out using the above cDNA as the template with primers designed in the 5’- and 3’-untranslated regions (UTR ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were processed for DNA library preparation (ThruPLEX DNA-seq Kit, Takara Bio), cleaned up with AMpure XP beads (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was filtered using a 0.25 µm syringe filter and applied to Talon metal affinity resin (Takara) in 50mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... The digested DNA was self-ligated using T4 DNA ligase (Takara, Japan). The ligated product was precipitated using 3M sodium acetate and 100% ethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1x M buffer containing 160 units of Nhe I (TaKaRa) overnight at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.025 μL recombinant RNase inhibitor (Takara, 2313B), 0.04 μL reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.02 μL recombinant RNase inhibitor (Takara, 2313B) and 2.21 μL nuclease-free water per reaction (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-STEM121 (Takara, Y40410, 1:250), goat anti-ChAT (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The CmR gene was amplified from plasmid pGro7 (Takara Bio) using the primer pair Cm-Vini-F/Cm-Vini-R ...
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bioRxiv - Biochemistry 2024Quote: ... After addition of DNaseI 1mM MgCl2 and 200μl 50% TALONR beads slurry (Clontech), the cell lysate was incubated at 10min at 4°C on a rolling shaker ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was reverse transcribed to cDNA with the PrimeScript RT kit (TaKaRa). cDNA was analysed by real-time PCR with the Light Cycler 480 SYBR Green I Master Mix (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... The pmEOS2-C1-ERGIC53 plasmid was constructed using using the In-Fusion® Snap Assembly Cloning Kit (Takara) and transformed into OmniMAX competent cells ...
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bioRxiv - Neuroscience 2024Quote: ... in BrainPhys neuronal media (composed according to Bardy et al. 2015)74 and 2 µg/ml doxycycline (Takara 631311). Three days after plating and ...
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bioRxiv - Neuroscience 2024Quote: ... and iMatrix (T304, TaKaRa) coated µ- dishes (Cat ...
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bioRxiv - Neuroscience 2024Quote: ... Human KCNQ2 cDNA (GenBank accession number NM_172108) in pIRES2-EGFP (BD Biosciences-Clontech, Mountain View, CA, USA) was used as template for in vitro mutagenesis ...
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bioRxiv - Molecular Biology 2024Quote: RNAi-Ready pSIREN-RetroQ vector (Clontech, Inc. Mountain View, CA, USA) was used to prepare shRNA constructs targeting Selenon gene (KD1 ...
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bioRxiv - Molecular Biology 2024Quote: ... These constructs were transfected into the 293 Ecopack packaging cell line (Clontech) using Lipofection 2000 transfection reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.25 µL of ExTaq (TaKaRa), 1× ExTaq buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Agarose gel electrophoresis was performed on a Mupid EX system (Takara) in 400 mL of 1× MOPS buffer at 20 V for 20 min and then at 100 V until the bromophenol blue dye had migrated approximately 2/3 of the gel ...
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bioRxiv - Molecular Biology 2024Quote: ... YCplac22 plasmids that carry tsa1 mutant alleles were constructed using the PrimeSTAR Mutagenesis Basal Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivirus titration was determined using Lenti-X p24 Rapid Titre Kit (Takara #631476) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293T cells (Clontech, Mountain View, CA, USA) were grown in 24 well dish to 50%– 60% confluency ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and cDNA was synthesized from the extracted RNA using PrimeScriptTM RT Reagent Kit (Takara Bio Inc., Japan) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: ... together with three DNA markers (λ-Hind III digest; Takara Cat. No. 3403, DL15,000 DNA Marker; Takara Cat ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were then run on a 4-12% Bis-Tris gel and transferred to a membrane for western blotting using α-GFP (Clontech) and α-HA (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2024Quote: ... the relevant combinations of constructs were co-transformed into the auxotrophic yeast strain AH109 using the polyethylene glycol/lithium acetate method according to the manufacturer’s manual (Clontech). Yeast cells were dotted on the plates of double (-Leu-Trp ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... with the PCR product of the W56-EGFP-Linker-Synapsin1a region from pAAV-hSyn1-W56-EGFP-Linker-Synapsin1a (FWD and REV primers: 5’-GGCGCGCCCTAGAATTTCAGTCGGAGAAGAGGCTGGC-3’ and 5’-ATGCTAGGCCACCATGATGGTGGACGGCAAGCCC-3’, respectively) using In-Fusion cloning (TaKaRa Bio). pAAV-hSyn1-DIO-Scr-EGFP-Linker-Synapsin1a was generated by replacing the TurboID region of pAAV-hSyn1-DIO-TurboID (EcoRI/NcoI sites ...
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bioRxiv - Plant Biology 2024Quote: ... 1.6 μL dNTP (TAKARA, Cat. No. 4030), 0.32 μL DIG-11-dUTP (25 nmol ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μg of purified total RNA was treated with RNase-free DNaseI (Takara, Dalian, China) to remove contaminated genomic DNA ...
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bioRxiv - Plant Biology 2024Quote: ... All PCR reactions were performed using the PCR components from Takara bio Inc ...
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bioRxiv - Plant Biology 2024Quote: ... the AtTPT gene was first amplified by PCR and then introduced into the pMD19-T vector (TaKaRa, Dalian, China) to construct pMD19-T-AtTPT ...
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bioRxiv - Plant Biology 2024Quote: ... The size ranges from 27 nt to 30 nt was cut and thus obtained RNA fragments were subjected into library generation using Smarter smRNA-Seq kit (Takara Cat# 635031).
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bioRxiv - Plant Biology 2024Quote: ... were enriched from the lysate by immunoprecipitation using GFP-Trap (Lablead, GNM-25-1000) and were partially digested by micrococcal nuclease (2 × 10−5 U/μL, Takara, 2910A). The digested RNA was ligated to the 3′-RNA adaptor labeled by biotin ...
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bioRxiv - Plant Biology 2024Quote: ... into chemically competent Saccharomyces cerevisiae Y2HGold cells (Takara Bio USA). Following transformation ...
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bioRxiv - Plant Biology 2024Quote: We utilized the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA) to investigate the interactions between HMA domain-containing proteins and Pwl2 ...
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bioRxiv - Plant Biology 2024Quote: ... were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech) to determine interactions between the two proteins.
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bioRxiv - Physiology 2024Quote: ... 10 μM Reverse primer and TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara, #RR820W). Real-Time PCR was set up in a 384-well plate and run in a CFX384 Real-Time PCR System (BioRad ...
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bioRxiv - Physiology 2024Quote: cDNA synthesis and Real-Time PCR were performed following the manufacture instructions of the PrimeScript RT reagent Kit with gDNA Eraser kit (Takara, #RR047A). For the Real-Time PCR reaction ...