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Citations for Takara Bio :
101 - 150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... At 48 hours the viral supernatant was filtered through a 0.45μm PVDF filter and concentrated using the Lenti-X concentrator (Takara, #631231) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T cells (Takara Bio #632180) were seeded at approximately 7e5 cells/well in a 6-well plate to yield ∼80% confluency the following day ...
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T packaging cells (Clontech #11131D) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2024Quote: ... Polymerase chain reaction (PCR) was performed using Primer STAR Max DNA Polymerase (Takara) to amplify the target gene ...
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T packaging cells (Clontech #11131D) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2024Quote: ... the viral supernatant was filtered through a 0.45μm PVDF filter and concentrated using the Lenti-X concentrator (Takara #631231) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: WT Human Embryonic Kidney (HEK) 293 cells (ATCC, catalog no. CRL-1573) and HEK293T-LentiX (Takara Biosciences, catalog no. 632180) were cultured in a humidity-controlled incubator under standard culture conditions (37 °C with 5% CO2 ...
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T packaging cells (Clontech #11131D) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 U/uL RNase inhibitor (Clontech, Mountain View, CA, USA), 0.1 mg/mL T7 polymerase (made in house following the protocol from Swartz et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... has been acquired from Takara Bio.
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bioRxiv - Synthetic Biology 2024Quote: ... The mixture (1 μL) was used as the template in 10 μL of PrimeSTAR GXL DNA Polymerase (Takara Bio Inc., Japan) reaction ...
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bioRxiv - Synthetic Biology 2024Quote: Cells were treated with Doxycycline (Clontech), Shield1 (MedCehmExpress ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2/9 particles were purified using the AAV Purification kit (Takara, Japan). The AAV solution was concentrated to the optimal volume by centrifugation using an Amicon Ultra-4 100k centrifugal filter unit (Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... and then transformed in Escherichia coli HST08 competent cells (Takara). The sequence integrity of inserts carried by transformants were verified by Sanger sequencing ...
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bioRxiv - Synthetic Biology 2024Quote: Total RNA was extracted using RNAiso plus (Takara) 48-72h after transient transfection or lentivirus infection ...
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bioRxiv - Synthetic Biology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). Amplified products were sequenced and verified after cloning into a pJET1.2-T vector through CloneJET PCR Cloning Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 100 ng pEGFP-N1 (Clontech). (4 ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was processed with dpn I (Takara Bio Inc.) to digest the template plasmid and then transformed into E ...
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bioRxiv - Genomics 2024Quote: ... together with three DNA markers (λ-Hind III digest; Takara Cat ...
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bioRxiv - Genomics 2024Quote: The Iso-Seq libraries were constructed from 1 µg of total RNA per sample and full-length cDNA were then generated using the SMARTer PCR cDNA synthesis kit (Takara Bio Inc, 639506). The libraries were sequenced on the Sequel Instrument v1 ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA encoding ZP3 was restored from the mouse ovarian tissue by RT-PCR using PrimeScript RT Reagent Kit (Takara, RR037). Two PCR amplicons including the 5′ region of the TECTA-ZP and the transmembrane domain (TMD ...
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bioRxiv - Developmental Biology 2024Quote: ... 500 ng of RNA template was subjected to in vitro reverse transcription using PrimeScript RT Reagent Kit (Takara, RR037). After elution with 40 ul of DNase/RNAse free water ...
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bioRxiv - Genetics 2024Quote: ... purified by phenol chloroform extraction and then reverse transcribed with a PrimeScript™ RT Reagent Kit (TAKARA, Dalian, China) according to the manufacturers’ protocol.
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bioRxiv - Zoology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). The PCR procedure was set as follows ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid techniques were performed according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany) using the yeast reporter strains AH109 and Y187 ...
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bioRxiv - Plant Biology 2024Quote: ... CIAP (TaKaRa, 2250B) was added and incubated at 37°C for 3 h ...
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bioRxiv - Plant Biology 2024Quote: ... using the In-Fusion Cloning system (Takara-Bio).
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bioRxiv - Plant Biology 2024Quote: ... SYBR Premix ExTaqTM (TaKaRa, Dalian, China), ABI 7500 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand complementary DNA (cDNA) was synthesized using a PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Dalian, China). SYBR Premix ExTaqTM (TaKaRa ...
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bioRxiv - Plant Biology 2024Quote: ... following standard procedures (Yeast Protocols Handbook PT3024-1, Clontech). The proteins were extracted by post-alkaline methods59 and detected by western blot with antibody Myc (Abmart ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast two-hybrid assays were performed using yeast strain AH109 (Clontech) following standard procedures (Yeast Protocols Handbook PT3024-1 ...
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bioRxiv - Plant Biology 2024Quote: The Y1H assays were conducted according to the manufacturer’s instructions (Matchmaker Gold Y1H Library Screening System; Clontech Laboratories, Mountain View, CA, USA). The pGADT7 empty vector with the pHis2 promoter and pGADT7-ZmHsf20 with the pHis2 empty vector were used as negative controls ...
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bioRxiv - Plant Biology 2024Quote: ... The qRT-PCR analysis was performed using the ABI StepOne Plus PCR system and the SYBR Green Realtime PCR mix (Takara). The ubiquitin gene (Lj5g3v2060710 ...
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bioRxiv - Plant Biology 2024Quote: ... The genomic DNA sequences surrounding the potential off-target sites selected for further analysis (Table 2) were amplified by PCR using specific primers (Supplementary Table S1) and PrimeSTAR GXL DNA Polymerase (Takara) from CRISPR/Cas-edited hairy roots (R1 of E-CP1 [the main root and five lateral roots] ...
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bioRxiv - Plant Biology 2024Quote: ... The quantitative RT-PCR was performed on a Thermal Cycler Dice® TP800 (TaKaRa) with the SYBER® Premix Ex Taq TM II kit (TaKaRa ...
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bioRxiv - Plant Biology 2024Quote: ... light-grown seedlings using the RNAiso plus reagent (TaKaRa). First strands were synthesized using PrimeScript® 1st Strand cDNA Synthesis Kit (TaKaRa) ...
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bioRxiv - Plant Biology 2024Quote: The yeast two-hybrid assays were carried out using a mating-based split ubiquitin system (mbSUS) for membrane protein interactions as described “mbSUS tests protocol” (Clontech) (Obrdlik et al. ...
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bioRxiv - Neuroscience 2024Quote: ... using infusion cloning (Takara Bio). This step swapped the FLEX cassette of pAAV-EF1a-FLEX-GTB for the FLEX [IRES-GFP] cassette ...
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bioRxiv - Synthetic Biology 2024Quote: ... was used and the synthesized mRNAs were purified with NucleoSpin RNA Plus (Takara). The concentration of mRNA was measured by Nano Drop One (Thermo Fisher scientific ...
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bioRxiv - Immunology 2024Quote: LDH in cell supernatants was measured using the LDH Cytotoxicity Kit (TaKaRa) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The SYBR Premix Ex Taq (TaKaRa, no. RR420DS) was used to perform real-time qPCR with ROX as a reference dye on a StepOne Plus Real-Time PCR System (Life Technology) ...
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bioRxiv - Plant Biology 2024Quote: The promoter sequences of STM-orthologs were isolated by TAIL-PCR (TAKARA, no.6108) from the DNA of the representative Brassicaceae species (Extended Data Table 1) ...
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bioRxiv - Plant Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed using SMART® MMLV Reverse Transcriptase (Clontech, www.clontech.com) using oligo(dT)15 primers ...
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bioRxiv - Plant Biology 2024Quote: ... All probes were filtered on columns (CHROMA SPIN-30 columns, Clontech) to remove remaining nucleotides ...
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bioRxiv - Plant Biology 2024Quote: ... 0,77g/L SD-URA drop out mix (Clontech), 0.67 g/L Yeast Nitrogen Base (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2024Quote: ... the VRN5 genomic region including endogenous promoter and terminator were amplified from genomic Col-0 DNA and cloned into pENTR using In-Fusion cloning (Takara). SYFP2 was then inserted with In-Fusion cloning ...
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bioRxiv - Synthetic Biology 2024Quote: ... 23S rRNA mutations were introduced to the pLK35 plasmid using the corresponding primer set (Supplementary Table 5) and the In-Fusion Cloning kit (Takara Bio). All sequences were confirmed with full plasmid nanopore sequencing (Plasmidsaurus and Elim Bio).
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Mutations in HUA2 restore flowering in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutantbioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using TB Green™ Premix Ex Taq™ II (Takara, Dalian, China). Relative gene expression was calculated using the 2−ΔΔCt method (Rao et al. ...
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bioRxiv - Plant Biology 2024Quote: ... A glutathione sepharose beads (635608, Takara)-based purification method was then used to purify the fusion protein ...
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bioRxiv - Plant Biology 2024Quote: ... and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser (Agilent) ...