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Citations for Takara Bio :
101 - 150 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: Total RNA was extracted using RNAiso plus (Takara) 48-72h after transient transfection or lentivirus infection ...
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bioRxiv - Synthetic Biology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). Amplified products were sequenced and verified after cloning into a pJET1.2-T vector through CloneJET PCR Cloning Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 100 ng pEGFP-N1 (Clontech). (4 ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was processed with dpn I (Takara Bio Inc.) to digest the template plasmid and then transformed into E ...
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bioRxiv - Genomics 2024Quote: ... together with three DNA markers (λ-Hind III digest; Takara Cat ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA encoding ZP3 was restored from the mouse ovarian tissue by RT-PCR using PrimeScript RT Reagent Kit (Takara, RR037). Two PCR amplicons including the 5′ region of the TECTA-ZP and the transmembrane domain (TMD ...
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bioRxiv - Developmental Biology 2024Quote: ... 500 ng of RNA template was subjected to in vitro reverse transcription using PrimeScript RT Reagent Kit (Takara, RR037). After elution with 40 ul of DNase/RNAse free water ...
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bioRxiv - Genetics 2024Quote: ... purified by phenol chloroform extraction and then reverse transcribed with a PrimeScript™ RT Reagent Kit (TAKARA, Dalian, China) according to the manufacturers’ protocol.
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bioRxiv - Zoology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). The PCR procedure was set as follows ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid techniques were performed according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany) using the yeast reporter strains AH109 and Y187 ...
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bioRxiv - Plant Biology 2024Quote: ... using the In-Fusion Cloning system (Takara-Bio).
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bioRxiv - Plant Biology 2024Quote: ... SYBR Premix ExTaqTM (TaKaRa, Dalian, China), ABI 7500 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand complementary DNA (cDNA) was synthesized using a PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Dalian, China). SYBR Premix ExTaqTM (TaKaRa ...
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bioRxiv - Plant Biology 2024Quote: ... following standard procedures (Yeast Protocols Handbook PT3024-1, Clontech). The proteins were extracted by post-alkaline methods59 and detected by western blot with antibody Myc (Abmart ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast two-hybrid assays were performed using yeast strain AH109 (Clontech) following standard procedures (Yeast Protocols Handbook PT3024-1 ...
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bioRxiv - Plant Biology 2024Quote: The Y1H assays were conducted according to the manufacturer’s instructions (Matchmaker Gold Y1H Library Screening System; Clontech Laboratories, Mountain View, CA, USA). The pGADT7 empty vector with the pHis2 promoter and pGADT7-ZmHsf20 with the pHis2 empty vector were used as negative controls ...
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bioRxiv - Plant Biology 2024Quote: ... The qRT-PCR analysis was performed using the ABI StepOne Plus PCR system and the SYBR Green Realtime PCR mix (Takara). The ubiquitin gene (Lj5g3v2060710 ...
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bioRxiv - Plant Biology 2024Quote: ... The genomic DNA sequences surrounding the potential off-target sites selected for further analysis (Table 2) were amplified by PCR using specific primers (Supplementary Table S1) and PrimeSTAR GXL DNA Polymerase (Takara) from CRISPR/Cas-edited hairy roots (R1 of E-CP1 [the main root and five lateral roots] ...
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bioRxiv - Plant Biology 2024Quote: ... The quantitative RT-PCR was performed on a Thermal Cycler Dice® TP800 (TaKaRa) with the SYBER® Premix Ex Taq TM II kit (TaKaRa ...
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bioRxiv - Plant Biology 2024Quote: ... light-grown seedlings using the RNAiso plus reagent (TaKaRa). First strands were synthesized using PrimeScript® 1st Strand cDNA Synthesis Kit (TaKaRa) ...
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bioRxiv - Plant Biology 2024Quote: The yeast two-hybrid assays were carried out using a mating-based split ubiquitin system (mbSUS) for membrane protein interactions as described “mbSUS tests protocol” (Clontech) (Obrdlik et al. ...
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bioRxiv - Neuroscience 2024Quote: ... using infusion cloning (Takara Bio). This step swapped the FLEX cassette of pAAV-EF1a-FLEX-GTB for the FLEX [IRES-GFP] cassette ...
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bioRxiv - Synthetic Biology 2024Quote: ... was used and the synthesized mRNAs were purified with NucleoSpin RNA Plus (Takara). The concentration of mRNA was measured by Nano Drop One (Thermo Fisher scientific ...
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bioRxiv - Immunology 2024Quote: LDH in cell supernatants was measured using the LDH Cytotoxicity Kit (TaKaRa) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The SYBR Premix Ex Taq (TaKaRa, no. RR420DS) was used to perform real-time qPCR with ROX as a reference dye on a StepOne Plus Real-Time PCR System (Life Technology) ...
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bioRxiv - Plant Biology 2024Quote: The promoter sequences of STM-orthologs were isolated by TAIL-PCR (TAKARA, no.6108) from the DNA of the representative Brassicaceae species (Extended Data Table 1) ...
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bioRxiv - Plant Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed using SMART® MMLV Reverse Transcriptase (Clontech, www.clontech.com) using oligo(dT)15 primers ...
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bioRxiv - Plant Biology 2024Quote: ... All probes were filtered on columns (CHROMA SPIN-30 columns, Clontech) to remove remaining nucleotides ...
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bioRxiv - Plant Biology 2024Quote: ... 0,77g/L SD-URA drop out mix (Clontech), 0.67 g/L Yeast Nitrogen Base (Sigma-Aldrich) ...
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Mutations in HUA2 restore flowering in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutantbioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using TB Green™ Premix Ex Taq™ II (Takara, Dalian, China). Relative gene expression was calculated using the 2−ΔΔCt method (Rao et al. ...
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bioRxiv - Plant Biology 2024Quote: ... A glutathione sepharose beads (635608, Takara)-based purification method was then used to purify the fusion protein ...
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bioRxiv - Plant Biology 2024Quote: ... and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser (Agilent) ...
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bioRxiv - Biochemistry 2024Quote: ... Mutations were introduced into the H-segment by site-directed mutagenesis with PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga, Japan). Plasmids were purified using a ZymoPURE Midiprep Kit (Zymo Research ...
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bioRxiv - Biochemistry 2024Quote: ... Mutations were introduced by site-directed mutagenesis with PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga, Japan). Biochemical measurements of the membrane integration of TMD6 were carried out using a previously described pGEM vector containing modified leader peptidase (Lep) ...
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bioRxiv - Biochemistry 2024Quote: ... The V276T and W107A mutations were also introduced by site-directed mutagenesis with PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga, Japan).
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bioRxiv - Biochemistry 2024Quote: ... an attB recombination site was inserted in place of the CMV promoter by InFusion HD Cloning (Takara Bio, Shiga, Japan). To facilitate the creation of these libraries using nicking mutagenesis ...
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bioRxiv - Biochemistry 2024Quote: ... a BbvCI restriction site was introduced by site-directed mutagenesis using PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga, Japan). The V276T and W107A mutations were also introduced by site-directed mutagenesis with PrimeSTAR HS DNA Polymerase (Takara Bio ...
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bioRxiv - Plant Biology 2024Quote: ... We then performed first-strand synthesis using the SMARTer Stranded Total RNA Kit V2- Pico Input Mammalian (Takara). Following purification with RNAclean XP (Beckman Coulter) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNAisoPlus was purchased from Takara (Otsu, Japan). HiScriptTM RT SuperMix for qPCR and AceQ qPCR SYBR Green Master Mix were obtained from Vazyme Biotech Co ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 2 ug of the total RNA from each sample were used to synthesize the cDNA using PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... HepG2-NTCP and Huh-7 cells with RNAi plus (TaKaRa, Japan) according to the manufacture’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The untagged version was made by cloning the RBP2 cDNA from this plasmid and replacing RIBEYE-GFP in the pAAV-CMV-HBA-RIBEYE-GFP-WPRE plasmid by in-fusion cloning (Takara Bio USA, Inc.).
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bioRxiv - Pathology 2024Quote: ... FAP and β-actin mRNA expression were determined using TB Green Premix Taq (Takara Bio). The primers used for β-actin were as follows ...
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bioRxiv - Genetics 2024Quote: ... into DH5α Electro-Cells (TaKaRa). The electroporated cells were spread on LB plates containing 12.5 mg/l chloramphenicol (Cm) ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was prepared from 1.5 μg of total RNA using the PrimeScript 1st strand cDNA Synthesis Kit (Takara). PCR reactions were performed with 1 μl of a 1:4 dilution of cDNA in ddH2O ...
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bioRxiv - Plant Biology 2024Quote: ... was done using the SMARTer Stranded Total RNA v2 kit (Takara), according to protocol ...
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bioRxiv - Genomics 2024Quote: ... together with three DNA markers (λ-Hind III digest; Takara Cat. No. 3403, DL15,000 DNA Marker; Takara Cat ...
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bioRxiv - Pathology 2024Quote: ... and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined using Agilent 2100 BioAnalyzer (Agilent Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... PXGL or UXGL medium was made from N2B27 medium (Takara, Y40002), supplemented with either 1 µM PD0325901 (Cambridge Bioscience ...
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bioRxiv - Plant Biology 2024Quote: ... facilitated by ExTaq polymerase (TaKaRa), was conducted in a 10 μl volume ...