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Citations for Takara Bio :
2701 - 2750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... mRNA was reversely transcribed to cDNA by PrimeScriptTMⅡ1st Strand cDNA Synthesis Kit (Takara-Clontech, Kyoto, Japan). The projection-specific neurons was aspirated into a pipette and then expelled into a PCR sterile tube containing 1 µl oligo-dT Primer and 1 µl dNTP mixture ...
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bioRxiv - Neuroscience 2023Quote: mRNA was reversely transcribed to cDNA by PrimeScriptTMⅡ1st Strand cDNA Synthesis Kit (Takara-Clontech, Kyoto, Japan). At the end of electrophysiological recordings ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was reversely transcribed to cDNA by PrimeScriptTMⅡ1st Strand cDNA Synthesis Kit (Takara-Clontech, Kyoto, Japan). The projection-specific neurons was aspirated into a pipette and then expelled into a PCR sterile tube containing 1 µl oligo-dT Primer and 1 µl dNTP mixture ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (qPCR) is the final laboratory step of the scRT-qPCR workflow with the SYBR Green Master Mix (TaKaRa).
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bioRxiv - Neuroscience 2023Quote: mRNA was reversely transcribed to cDNA by PrimeScriptTMⅡ1st Strand cDNA Synthesis Kit (Takara-Clontech, Kyoto, Japan). At the end of electrophysiological recordings ...
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bioRxiv - Biochemistry 2023Quote: ... 1 volume of Lenti-X concentrator (Clontech, 631231) was added to 3 volumes of clarified-lentivirus containing medium ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified cell lysate was incubated with pre-equilibrated Ni- NTA resin (Takara) for 2 h at 4°C in slow shaking conditions ...
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bioRxiv - Biochemistry 2023Quote: Mouse UCP1 gene and human codon-optimized mitoLbNOX and LbNOX (addgene #74448 and #75285) were cloned into pLVX-TRE3G vector (Clontech, CA) using Gibson assembly ...
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bioRxiv - Biochemistry 2023Quote: ... and genomic DNA was removed using DNase I (Takara, Beijing, China). RNA concentrations ranged from 416 to 845 ng μL-1 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant (800 µL) was incubated with 30 µL of Talon beads (Takara Bio) at 4°C for 1 hour with continuous shaking ...
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bioRxiv - Biochemistry 2023Quote: ... 1400 ng of pLVX-TRE3G vector of interest (including pLVX-TRE3G-Luc control vector, expressing Luciferase, purchased from Clontech) and Opti-MEM medium (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... ∼5 μg of bacmid were transfected using 5 μL of TransIT®-Insect transfection reagent (Takara Bio Inc.). 5 days after initial transfection ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories, Inc.). The cloning mixtures were transformed to One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Biochemistry 2023Quote: ... 425 – 658) and SipAC-Core (a.a. 512 – 658) were cloned with an N-terminal 6xHis-tag into pColdI vector (Takara Bio USA) modified to include a tobacco etch virus (TEV ...
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bioRxiv - Biophysics 2023Quote: ... 5’-CTGGAGAATCCCGGTGC-3’ and 5’- GTGTCAGATATATACATCCTGT-3’ and the PCR product was purified using a NucleoSpin Gel and PCR Clean-up Maxi kit (Takara Bio). The sequences of the 147 bp and 177 bp DNA are as follows:
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bioRxiv - Microbiology 2023Quote: ... 1 U/μL RNAse inhibitor (cat. no. 2313A, Takara), 5 U/μL SuperScript™ II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... 1 U/μL RNAse inhibitor (cat. no. 2313A, Takara), 2 mM dNTPs (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... 400 ng of RNA was used for reverse transcription using PrimeScript RT Master Mix (Takara, USA). PCR reactions were performed using TB Green Premix Ex Taq (Takara ...
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bioRxiv - Neuroscience 2023Quote: ... the Aprt cDNA was subcloned into the pUASTattB vector (Bischof et al. 2007) using the In-Fusion HD Cloning Kit (Takara Bio, Kyoto, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA was isolated by standard procedure from 20-30 heads of homozygous Aprt5 flies and reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto, Japan). At least 750 ng of the first strand cDNA was amplified by PCR in 20 μl of reaction mixture using PrimeStar Max DNA polymerase (Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... At least 750 ng of the first strand cDNA was amplified by PCR in 20 μl of reaction mixture using PrimeStar Max DNA polymerase (Takara Bio, Kyoto Japan) with a Techne Prime Thermal Cycler apparatus (Bibby Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... at least 750 ng of the first strand cDNA was amplified in 20 μl of reaction mixture using PrimeStar Max DNA polymerase (Takara Bio, Kyoto Japan) with a Techne Prime Thermal Cycler apparatus (Bibby Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The qPCR reaction was carried out using Probe qPCR Mix kit (Takara) ...
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bioRxiv - Bioengineering 2023Quote: ... The qPCR reaction was carried out using Probe qPCR Mix kit (Takara). For each sample ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using the SMARTer PCR cDNA Synthesis Kit (Takara Bio Group) and sequenced using the Illumina HiSeq 2000 sequencer at the Beijing Genomics Institute (BGI ...
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bioRxiv - Plant Biology 2023Quote: ... between the pU6 promoter and sgRNA scaffold via Bsa I digestion and T4 DNA ligase-mediated ligation (Takara, cat. 6023). For oligonucleotide-dependent mutation knock-in ...
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bioRxiv - Immunology 2023Quote: ... The pLVX-zsGreen lentiviral vector and Retro-X Universal Packaging System were purchased from Takara Bio (San Jose ...
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bioRxiv - Immunology 2023Quote: Ultra-low input RNA sequencing libraries (from sorted Tgfb1fl/fl;Cx3cr1Cremicroglia) were prepared by using SMART-Seq HT kit for full-length cDNA synthesis and amplification (Takara, San Jose, CA, USA), and Illumina Nextera XT (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... All RT-qPCR reactions were carried out in triplicate using ThunderBird SYBR qPCR Mix (TOYOBO) and Thermal Cycler Dice Realtime System (TAKARA), and normalized to mRNA expression level of human ribosomal protein L13A ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... to OD600 0.2–0.3 with 2–3 mL fresh AYE +Fe +Cys containing 40 ng/mL anhydrous tetracycline (aTC, Clontech 631310). On the third day ...
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bioRxiv - Microbiology 2023Quote: ... hcaA was amplified by PCR and introduced into pCold™ I DNA (TaKaRa, Shiga, Japan) using the In-Fusion® HD Cloning Kit ...
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bioRxiv - Plant Biology 2023Quote: ... we PCR amplified (CloneAMP HIFI PCR premix, TAKARA) the coding sequences of ATG8a ...
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bioRxiv - Plant Biology 2023Quote: ... ARF7 and TSPO flanked recognition sequence for Gateway (Thermofischer) or In-Fusion (Clontech) cloning ...
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bioRxiv - Cell Biology 2023Quote: ... pmCherry-C1(Picard et al., 2006) and pEGFP-C1 (Clontech #6084-1) were used as respective vector control ...
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bioRxiv - Physiology 2023Quote: The fluorescent marker proteins mCherry and GFP expressed in response to the viral infections and the cholinergic marker enzyme Chat were detected by a triple-label immunofluorescent technique using rabbit anti-DS Red IgG (Clontech, #632496), chicken anti-GFP (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and extension were all completed with the SMARTer PCR cDNA Synthesis Kit (Takara Bio Europe, France). Full-length cDNA (fl-cDNA ...
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bioRxiv - Developmental Biology 2023Quote: ... YPD medium (Clontech), and Potato Dextrose Broth (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... In short: 3.5 cm non-coated cell culture dishes (Falcon, cat 351008) were coated using 12 µg Retronectin (Takara, cat T100B). Coated dishes then were pre-incubated with 1 ml viral supernatant for 4-5 hours at 37oC ...
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bioRxiv - Neuroscience 2023Quote: ... Viral supernatant was concentrated using Lenti-X concentrator (Takara Bio) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were prepared using the SMARTer v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA). Sequencing was performed on NovaSeq S4 150PE platform (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were performed using TB Green Premix Ex Taq (Takara, USA). Gene expression was normalized against the average of two loading controls (Btf3 and Ppia).
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bioRxiv - Neuroscience 2023Quote: ... 5 μL of treated RNA was reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto Japan). Then ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed with 2x SYBR green mix (#RR420L, Takara Bio, Otsu, Shiga, Japan) and primers against JAK1 ...
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bioRxiv - Microbiology 2023Quote: ... with PrimeStar Taq DNA Polymerase (Takara, cat#R045A). Concentrations were measured using the Qubit dsDNA HS (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: Mammalian expression constructs were mainly generated by Gibson assembly of PCR-amplified coding sequences (primers, Supp. Data File 2) into restriction-digested pC1 (CMV promoter, modified from pEGFP-C1, Clontech) or pCAG (CMV enhancer fused to chicken beta-actin promoter ...
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bioRxiv - Microbiology 2023Quote: ... Viral cDNA was PCR amplified using CloneAmp HiFi (TaKaRa) in four overlapping amplicons of about 2.2 kb ...
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bioRxiv - Genomics 2023Quote: ... We prepared the adult brain total RNA using the Clontech SmartSeq v4 Ultra-Low Input RNA Kit (Takara). We prepared libraries with the Nextera XT DNA Library Preparation Kit and 96-Sample Index Kit (Illumina ...
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bioRxiv - Genomics 2023Quote: ... PCR3 products (final library) were purified with the Nucleospin Gel and PCR Cleanup kit (Takara, 740609) and eluted in 25 µL 70 °C buffer.
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bioRxiv - Genomics 2023Quote: ... Products were purified with the Nucleospin Gel and PCR Cleanup Kit (Takara, 740609) with 1:5 buffer NTI dilution and 25 µL 70 °C elution buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and qPCRs were performed using TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara) according to the manufacturer’s protocol.