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Citations for Takara Bio :
2601 - 2650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Technical duplicates were performed with thirty-five cycles of PCR in a 50 μL reaction with 0.4 mM TprK forward and reverse primers (Table S4) using the 2x CloneAmp MasterMix (Takara) with a 62°C annealing temperature ...
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bioRxiv - Microbiology 2023Quote: ... the HCoV-OC43 N sequence was cloned into backbone vector pLVX-EF1alpha-2xStrep-IRES-Puro by In-Fusion cloning (Takara Bio, Inc.), obtaining pLVX-EF1alpha-OC43-N-2xStrep-IRES-Puro.
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bioRxiv - Microbiology 2023Quote: ... A 2x hot-start PCR master mix from Takara (cat#: R405A) was used for high-fidelity PCR amplification of the pBCG02 backbone ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were reverse-transcribed using Prime Script RT Reagent kit (TaKaRa) in combination with an AS1-specific tagged primer (no ...
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bioRxiv - Microbiology 2023Quote: ... followed by RT-PCR using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa) with primers no ...
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bioRxiv - Microbiology 2023Quote: ... followed by RNA extraction using NucleoSpin RNA Plus (TaKaRa).
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bioRxiv - Microbiology 2023Quote: ... followed by a PCR reaction using PrimeSTAR Max DNA Polymerase (TaKaRa) with primers no ...
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bioRxiv - Microbiology 2023Quote: ... The resultant fragments were cloned into the EcoRI and NotI recognition sites of pCAG neo using HD Cloning Kit (TaKaRa). All recombinant proteins were fused with a his-tag and expression was confirmed using an anti-his tag antibody.
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bioRxiv - Microbiology 2023Quote: RNA obtained from the RIP assay sample was purified using NucleoSpin RNA Plus (TaKaRa), followed by library construction and RNA-seq analysis ...
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bioRxiv - Microbiology 2023Quote: ... and were quantified by real-time RT-PCR using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (TaKaRa) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: The open reading frames of 19 LD-associated proteins were amplified from RIB40 genomic DNA with PrimeSTAR® HS DNA Polymerase (TaKaRa, Otsu, Japan) for high-fidelity PCR ...
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bioRxiv - Microbiology 2023Quote: ... anti-E-cadherin (clone ECCD-2) (Takara), goat anti-rat488 (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... Fragments encoding the CD45 extracellular domain (ECD) and transmembrane region were amplified by PCR and cloned into pEGFP-N1 (Clontech) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Microbiology 2023Quote: ... The retroviral reporter plasmids encoding a Gaussia luciferase reporter gene were constructed by cloning the reporter genes into the pQCXIP plasmid (Clontech). Plasmids encoding soluble ACE2 variants fused with human IgG1 Fc were described in our previous study20 ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription were conducted using a PrimeScript RT reagent Kit (TaKaRa, Dalian, China). HEV RNA from different samples was detected using 2 × RealStar Power SYBR Mixture (Genstar ...
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bioRxiv - Microbiology 2023Quote: ... a pmCherry plasmid (Clontech, Palo Alto, CA, USA, cat #632522) was electroporated into K ...
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bioRxiv - Microbiology 2023Quote: ... and pCMV-Rev into 293T cells using TranIT-293 (TaKaRa, Shiga, Japan) or the ViraPower Lentiviral Expression System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: The virus RNA in the cell or culture supernatant was extracted with nucleospin RNA plus (TaKaRa) and quantified by RT‒qPCR using Primer/Probe N2 (NIHON GENE RESEARCH LABORATORIES ...
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bioRxiv - Microbiology 2023Quote: ... and sgEHMT2 cells using nucleospin RNA plus (TaKaRa), and RT‒qPCR was performed using Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... coli HST08 (Takara Bio). The pLI50_pgl plasmid was then isolated and transformed by electroporation into the restriction-deficient strain RN4220 ...
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bioRxiv - Microbiology 2023Quote: ... a pEGFP-C1 (Clontech)-derived plasmid (5) ...
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bioRxiv - Microbiology 2023Quote: ... inverse PCR amplification was conducted with oligonucleotides and templates listed in Table S1 and the resulting PCR products were treated with T4 Polynucleotide Kinase (Takara) and ligated with a DNA ligation kit (Takara) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were pooled by template and purified (Nucleospin, Takara). RACE amplicons were end repaired ...
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bioRxiv - Neuroscience 2023Quote: ... were purified with the NucleoBond Xtra Midi EF kit (Takara 740420). Il6ra knockdown efficiency was assessed by transient transfection of sgRNA and dCas9-KRAB-MeCP2 into the mHypoA hypothalamic neural cell line (Cedarlane Labs ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... with SYBR® Premix Ex Taq (Tli RNase H Plus) by TAKARA. Each sample was amplified in triplicates using primers specific to genes of interest ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: Quadriceps muscles were dissected and snap-frozen in liquid nitrogen and homogenized in RNAiso plus Reagent (TAKARA, 1 mL/100 mg tissue) to isolate total muscle RNA as per the manufacturer’s recommendations ...
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bioRxiv - Physiology 2023Quote: ... cleaned up (NucleoSpin Gel and PCR cleanup, 740609.50, Takara Bio, Shiga, Japan), and transformed into pENTR vectors using a D-TOPO kit (45-0218 ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative RT-PCR was conducted on Bio-Rad CFX96 using the SYBR green PCR master mix (TaKaRa, Japan) with the primers listed in Appendix 1—key resources table ...
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bioRxiv - Neuroscience 2023Quote: ... which were then reversely transcribed by RT Master Mix according to the instruction (Takara, Japan). Finally ...
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bioRxiv - Physiology 2023Quote: ... cDNA was made from 100-400 ng of DNase-treated total RNA using a Takara PrimeScript RT Reagent Kit with gDNA Eraser (Takara bio RR047B). Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus ...
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bioRxiv - Physiology 2023Quote: ... Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus) (Takara bio RR820W) and a QuantStudio 6 Flex Real Time PCR system (Thermo Fisher ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: First-strand cDNA was synthesized from total RNA using the PrimeScript First-Strand cDNA Synthesis Kit with PrimeScript Reverse Transcriptase according to the manufacturer’s protocols (TAKARA). To 1 ug of RNA ...
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bioRxiv - Physiology 2023Quote: ... The adenovirus titer in plaque-forming units (PFU) was determined using a Adeno-X Rapid Titer kit (Clontech, Palo Alto, CA).
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were prepared from 1 ng of total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa) according to the manufacturer guidelines for cDNA synthesis and amplification ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from plant leaves using a MiniBEST Plant RNA Extraction Kit (TaKaRa, China). RNA (10–20 μg ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from plant tissue at the site of inoculation using a MiniBEST Plant RNA Extraction Kit (TaKaRa, China) as per kit instructions ...
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bioRxiv - Plant Biology 2023Quote: Genome walking was conducted using the Universal GenomeWalker 2.0 kit (Takara Bio) according to the manufacturer’s instructions to isolate GdMYBSG6-1 and GdMYBSG6-2 5’UTRs and the promoter regions of GdANS and GdMAT1 ...
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bioRxiv - Plant Biology 2023Quote: ... His8-tagged soluble or insoluble CML recombinant proteins were purified at room temperature using Ni-NTA resin (His60, Takara Bio Inc.) using the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... a high-fidelity DNA polymerase (PrimeSTAR GXL, Takara) and purified using a DNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Plant Biology 2023Quote: ... Poly-A tail enriched cDNA libraries were prepared using the SMART-Seq HT Kit (Takara Bio, Japan), quality checked using the TapeStation DNA ScreenTape (Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... These bait and prey vectors were co-transfected into yeast strain Y2H GOLD (Clontech). The transfected yeast cells were screened on SD/-Leu/-Trp ...
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bioRxiv - Plant Biology 2023Quote: The coding sequences of the genes were cloned into pCold-TF vector (TaKaRa) which contains a His tag ...
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bioRxiv - Plant Biology 2023Quote: ... or CloneAmpTM HiFi PCR Premix (Takara Bio) using the corresponding primers listed in TableS1 ...
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bioRxiv - Zoology 2023Quote: ... Primers were designed on each contig (Table S3) and RT-PCR was performed using a 3’ RACE CORE Set (Takara Bio).
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bioRxiv - Plant Biology 2023Quote: ... Synthesized cDNA was amplified by real-time quantitative PCR (qPCR) with TB Green Premix Ex Taq (Tli RNaseH Plus) (Takara) using the QuantStudio 3 system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoprecipitad DNA was analyzed by qPCR and reactions were performed in triplicate with TB Green Premix Ex Taq (Tli RNaseH Plus) (Takara) using the QuantStudio 3 system (Applied Biosystems) ...
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bioRxiv - Zoology 2023Quote: ... primers were designed on each scaffold using Primer3Plus (https://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi) (Table S3) and PCR was performed using PrimeSTAR Max DNA Polymerase (Takara Bio, Shiga, Japan). The sequences of the PCR products ...
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bioRxiv - Zoology 2023Quote: ... including stranded library prep and treatment with Ribogone™ (Takara Bio USA Inc., Mountain View, CA) was performed by the Emory National Primate Research Center Genomics Core on the Illumina HiSeq 3000 system (single-end strand specific reads ...