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Citations for Takara Bio :
2551 - 2600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... purified using the AAVpro purification kit (Takara), and the titer was determined using ddPCR ...
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bioRxiv - Neuroscience 2023Quote: ... and rabbit anti-mCherry (1:1000, TaKaRa Living Colors DsRed Polyclonal Ab 632496) ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR mixture contains 5 ul EmeraldAmp GT PCR Master Mix (Takara, #RR310B), 1 ul genomic DNA ...
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bioRxiv - Neuroscience 2023Quote: ... and rabbit anti-DsRed (1:500; Clontech, # 632496) and goat anti-tdTomato (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... SMARTer Stranded Total Sample prep kit-HI Mammalian (Takara, 634875) was used to generate libraries for RNA sequencing.
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bioRxiv - Neuroscience 2023Quote: ... the Low Input Library Prep Kit v2 (Clontech) was used ...
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bioRxiv - Neuroscience 2023Quote: ... the In-Fusion Cloning Tool (Takara-bio Inc.) was used to design primers to induce the desired mutation (substitution ...
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bioRxiv - Neuroscience 2023Quote: The site-directed mutagenesis kit (Takara-bio Inc.) was used to alter specific cis-regulatory regions ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μl of cloning enhancer (Takara-bio Inc.) was added to 5 μl of PCR reaction volume to remove the original plasmid ...
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bioRxiv - Neuroscience 2023Quote: ... A 10 μl In-Fusion Cloning reaction (Takara-bio Inc.) was prepared by adding 2 μl of 5X In-Fusion HD Enzyme Premix ...
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bioRxiv - Neuroscience 2023Quote: ... Medium containing lentiviral particles was concentrated using a Lenti-X concentrator (Takara Bio, Japan) and resuspended in Neurobasal (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% tetracycline-free FBS (Takara Bio, Cat. No. 631367), 100 U/mL Penicillin-Streptomycin (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was concentrated using Lenti-X Concentrator (Clontech, 631231), resuspended by one-twentieth volume of PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the insert was amplified using primers (Key Resources Table) designed with the In-Fusion Cloning Tool (Takara-bio Inc.). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... and transferred into 8 µL lysis buffer (Clontech SMARTer Ultra Low Input RNA Kit for Sequencing - v3) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used were rabbit anti-DsRed (1:500; #632496, Takara), rat anti-mCherry (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were sorted directly into buffer for SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Subsequent processing was carried out by the UCL Genomics sequencing facility ...
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bioRxiv - Neuroscience 2023Quote: ... Viral titers were estimated using Lenti-X GoStix Plus (Takara Bio, 631280) following the manufacturer’s instructions using a reference value of 6.014 (TU/ml)/GV ...
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bioRxiv - Neuroscience 2023Quote: Neurons were transfected at day in vitro (DIV) 7 using CalPhos Mammalian Transfection Kit (Takara Bio, 631312). Transfection solution was prepared by adding 1.5 µg DNA per construct (3 µg in total ...
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bioRxiv - Neuroscience 2023Quote: ... using InFusion cloning (Clontech, Cat#638909). hsynapsin and EGFP sequences were PCR amplified from pLenti-hSynI-EmGFP-WPRE described in (7) ...
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bioRxiv - Neuroscience 2023Quote: ... All cloning reactions used PCR amplification and ligation-independent cloning (In-Fusion, Takara Inc.). Reactions involving non-AAV plasmids were transformed into Stellar competent cells (Takara Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions involving non-AAV plasmids were transformed into Stellar competent cells (Takara Inc.) and those involving AAV plasmids were transformed into NEB Stable competent cells (New England Biolabs).
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bioRxiv - Neuroscience 2023Quote: ... 2 µg/ml doxycycline (Takara) was added on day 0 to induce TetO gene expression ...
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bioRxiv - Microbiology 2023Quote: ... and 12 U of recombinant RNase inhibitor (Takara Bio) in 1X T4 RNA Ligase Buffer (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 NL4-3 Vpu ORF was cloned into pEGFP-N1 (Clontech, France). The ORF of human ATG5 was cloned in frame with HA tag into pAS1B vector (pAS1B-HA-ATG5) ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 µg of total RNA was used for reverse transcription using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara, Beijing, China). After the transcribed cDNAs were 5-fold diluted ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit with nonstranded and polyA+ selection (Clontech, Takara Bio USA, Mountain View, CA, USA). Approximately 120 million 125-bp paired-end reads per sample were obtained (HiSeq 2500 ...
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bioRxiv - Molecular Biology 2023Quote: Library transformation was performed as described by Clontech (Yeast Protocols Handbook) except that a 1h recovery step at 30°C was inserted between heat shock at 42°C and plating on selective media (synthetic defined (SD ...
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bioRxiv - Molecular Biology 2023Quote: ... coated with SD-Leu/-trp (Clontech, USA) plate ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid pCMV-GFP-Zfp106 was created by cloning the Zfp106 cDNA into the XhoI-EcoRI sites in pEGFP-C2 (Clontech). Plasmid pCMV-RFP-Zfp106 was created by replacing EGFP in plasmid pCMV-GFP-Zfp106 with NLS-TagRFP ...
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bioRxiv - Molecular Biology 2023Quote: ... Monoclonal U-2OS cell lines stably expressing Tet-On 3G transactivator protein (Clontech), a tandem-dimeric MS2 hairpin binding protein tagged with eYFP (MS2CP-YFP) ...
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bioRxiv - Neuroscience 2023Quote: ... Native tdTomato fluorescence destroyed by combined ISH protocol was recovered by rabbit anti-DsRed (1:1000, Takara Bio Clontech # 632496 ...
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bioRxiv - Neuroscience 2023Quote: ... Native tdTomato fluorescence destroyed by combined ISH protocol was recovered by rabbit anti-DsRed (1:1000, Takara Bio Clontech # 632496, RRID: AB_10013483) antibody and switched to the green channel using an Alexa Fluor 488 secondary ...
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bioRxiv - Neuroscience 2023Quote: ... by using SYBR Premix Ex Taq TM (TAKARA, Japan) and parameters were ...
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bioRxiv - Neuroscience 2023Quote: ... or PrimeSTAR Mutagenesis Basal kit (Takara Bio Inc., Shiga, Japan): Y156V ...
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bioRxiv - Plant Biology 2023Quote: ... and converted to cDNA using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio), following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: ... Transcripts of selected genes were quantified using TB Green® Premix Ex Taq™ (Tli RNaseH Plus) (TaKaRa, RR420A) with Realplex2 (Eppendorf) ...
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bioRxiv - Plant Biology 2023Quote: ... using SYBR Premix Ex Taq (Takara) and primers at 300nM concentration ...
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bioRxiv - Plant Biology 2023Quote: ... The bisulfite-converted library was split between two 50 ul reactions and PCR amplified using the following conditions: 2.5 U of ExTaq DNA polymerase (Takara Bio), 5 μl of 10X Extaq reaction buffer ...
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bioRxiv - Plant Biology 2023Quote: ... an In-Fusion HD Cloning Kit (Clontech) was used ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized by using the Primescript RT reagent kit (Takara Bio, Japan). qPCR was performed in a LightCycler480 II (Roche Diagnostics International AG ...
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bioRxiv - Plant Biology 2023Quote: ... Stellar Escherichia coli HST08 cells (Takara Bio, Japan) and the Agrobacterium tumefaciens strain AGL1 were used for cloning and Z ...
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bioRxiv - Plant Biology 2023Quote: ... dNTP (Takara Bio), and the primers designed for the above-mentioned FWA regions (see Supplementary Table 5 ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was used as template for PCR using TaKaRa Ex Taq or PrimeSTAR GXL DNA polymerase (Takara Bio), according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... from transformed Stellar Competent Cells (Takara Bio Inc, Japan). The DNA was sequenced and verified (CLC Sequence Viewer 8 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... ExTaq polymerase (Clontech, 1.5 μL), dNTP mix (8 μL) ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative real-time was carried out using TB Green Premix Ex TaqII (TaKaRa) in CFX96 real-time PCR system (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... or Omicron variant (G339D) using a SARS- CoV-2 Direct Detection RT-qPCR Kit (TaKaRa Bio Inc., Shiga, Japan) with each specific primer/probe ...
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bioRxiv - Microbiology 2023Quote: ... LASVpp-BlaM viruses were concentrated ten times with Lenti- X concentrator (Clontech Laboratories, Mountain View, CA, USA), snap-frozen and stored at - 80 °C.
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription products were then subjected to qPCR with a commercial kit (TAKARA, Cat. No. RR820Q) to amplify GAPDH (Forward primer ...