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Citations for Takara Bio :
2501 - 2550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... trizol (Takara Bio) was added to the plate ...
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Antibodies targeting Crimean-Congo hemorrhagic fever virus GP38 limit vascular leak and viral spreadbioRxiv - Microbiology 2024Quote: ... In-Fusion enzyme (Takara Bio) was used to insert the gBlocks between the secretion signal and the constant region ...
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bioRxiv - Microbiology 2024Quote: ... as well as into pEGFP-C3 (Clontech Takara, Tokyo, Japan). The plasmid encoding the HCV core protein (amino acids 1-192 ...
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bioRxiv - Microbiology 2024Quote: ... Yeast cells containing pGBKT7PA28γ were grown in yeast extract-peptone-dextrose medium and transfected with a human foetal brain plasmid library based on pACT2 (Clontech Takara). Clones (22.4 × 106 ...
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bioRxiv - Microbiology 2024Quote: ... as well as into pEGFP-C3 (Clontech Takara, Tokyo, Japan). The plasmid encoding the HCV core protein (amino acids 1-192 ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble fraction obtained was applied to Talon beads ( Cat # 635503; Takara) and incubated for 6 h at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... hTRIM21 full-length CDS with N-terminal Myc and EGFP was cloned into pLEX-MCS and transfected into HEK293T-Lenti cells (Clontech 632180) along with pMD.2G and psPAX2 (Addgene ...
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bioRxiv - Biochemistry 2024Quote: PCR for the full-ORF amplification were performed with PrimeSTAR Max DNA Polymerase (TaKaRa Bio Inc. Shiga, Japan) following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... cerevisiae SH-4 cells using NucleoSpin RNA (Takara Bio, Otsu, Japan) and Quick-RNA MiniPrep Plus (Zymo Research The MGIEasy RNA Directional Library Prep Set (MGI Tech ...
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bioRxiv - Zoology 2024Quote: ... and reverse transcribed using the PrimeScriptTM RT reagent Kit with gDNA Eraser (Perfect Real Time) kit (Takara, Dalian, China). The RT-qPCR reactions were carried out on the PikoReal 96 Real-Time PCR System (Thermo ...
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bioRxiv - Molecular Biology 2024Quote: ... pTRE2hyg vector (Clontech, USA) encoding mutated hAT1 with impaired ability to activate β-arrestin (pTRE2hyg-HA-FLAG-hAT1mg ...
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bioRxiv - Systems Biology 2024Quote: ... in conjunction with the Mir-X™ miRNA qRT-PCR TB Green® Kit from TAKARA (San Jose, CA, USA). After confirming miRNA inhibition in tick tissues ...
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bioRxiv - Synthetic Biology 2024Quote: ... a non-TC treated 6 or 12-well plate was coated with 20 ug/mL Retronectin (Takara) in PBS and incubated at 4°C overnight ...
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bioRxiv - Synthetic Biology 2024Quote: Replication-incompetent lentivirus was packaged using HEK293T Lenti-X cells (Takara) and third generation lentiviral packaging and envelope plasmids ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was reverse transcribed from 1 μg of total RNA using the PrimeScriptTM RT reagent kit (AK4201; Takara Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: Libraries were prepared by the Van Andel Genomics Core (Grand Rapids, MI) from 1 ng of total RNA using Takara SMART-Seq Stranded Kit (Takara Bio USA, Mountain View, CA) per the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... and ExTaq (Takara, Shiga, Japan). The cycling conditions for the PCR included 30 cycles at 94 °C for 30 seconds ...
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GIBBERELLIN SIGNALING THROUGH RGA SUPPRESSES GCN5 EFFECT ON STAMEN ELONGATION OF ARABIDOPSIS FLOWERSbioRxiv - Plant Biology 2024Quote: ... The enzyme ExTaq DNA polymerase (Takara, Japan) was used with the primers listed in Supplemental Table S4 to confirm the double mutants ...
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bioRxiv - Plant Biology 2024Quote: ... Amplification of bisulfite converted DNA was performed using the TaKaRa EpiTaqTM HS (Takara), and the resulting PCR reactions were cleaned-up using the NucleoSpin Gel and PCR Clean-up XS kit (Macherey-Nagel) ...
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bioRxiv - Plant Biology 2024Quote: ... cDNAs were synthesized using PrimeScript RT Kit with gDNA Eraser (Perfect Real Time; Takara) following the protocol of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed using TB Green PCR Master Mix (RR820, Takara Bio) with the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of total RNA was amplified and converted into cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). About 17 fmol of cDNA was used for library preparation using the PCR Barcoding kit (SQK-PBK004 kit ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNA-sequencing libraries were made using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). Libraries were sequenced at the Bauer Core Facility (Harvard University ...
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bioRxiv - Molecular Biology 2023Quote: ... Input and eluted total RNA from m6A-IP were used to prepare libraries with Takara Pico-Input Strand-Specific Total RNA-seq for Illumina v2 (Takara). Sequencing was performed on Illumina Nova-seq according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized pUCHCPV-20-4 was used as a template for in vitro transcription by T7 RNA polymerase (TaKaRa, Dalian, China) to generate chimeric HDAC11-S4 RNA 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... and verified whether it has self-activation reaction through synthetic dropout (SD)-Ade-His-Leu-Trp (Clontech, CA, USA) with X-α-galactosidase (x-α-gal) ...
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bioRxiv - Molecular Biology 2023Quote: Microscopy constructs: NbV5-eGFP was generated by PCR amplification of NbV5 and cloned into pEGFP-N1 (Clontech) at the HindIII-BamHI sites.
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA was synthesized using PrimeScript™ RT reagent kit with gDNA Eraser (TaKaRa, Dalian, China). Primers used for qPCR (forward ...
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bioRxiv - Molecular Biology 2023Quote: ... and inserted between the EcoRI-SalI sites of pEGFP-C1 vector (Clontech) giving pBS5988 and pBS5528 ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/2000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... and 1ul recombinant RNase inhibitor (Takara, 2313B) incubated on ice for 5min ...
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bioRxiv - Molecular Biology 2023Quote: ... before being concentrated 10-fold using the Lenti-X concentrator (Takara) according to the manufacturers ‘ protocol.
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bioRxiv - Molecular Biology 2023Quote: ... culture medium was collected filtered and concentrated using the Lenti-X™ Concentrator (Takara). Viral particles were suspended in fresh DMEM medium and stored at - 80°C ...
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Mutations of MavH were introduced by in vitro site-directed mutagenesis using specific primers containing the defined base changes and PrimeSTAR® Max DNA Polymerase (Takara Bio, Inc.) premix ...
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bioRxiv - Molecular Biology 2023Quote: ... viruses were collected and concentrated using Lenti-X Concentrator (Takara, 631232).
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bioRxiv - Molecular Biology 2023Quote: ... 1x Titanium Taq DNA polymerase and buffer (Clontech). PCR cycling conditions used are ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 U/mL RNase inhibitor (TAKARA)) ...
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bioRxiv - Molecular Biology 2023Quote: ... The template was transcribed with T7 RNA Polymerase (TAKARA) and purified using Probe-Quant G-25 microcolumns (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish znf598 (ENSDARG00000014945) was amplified by RT-PCR and cloned into pCS2+ via XhoI/XbaI restriction sites using DNA Ligation Kit (TAKARA). To generate znf598 point mutation in ORF (znf598 C13/16A) ...
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bioRxiv - Molecular Biology 2023Quote: ... wild-type ORF was amplified using point mutated primers and cloned into pCS2+ via XhoI/XbaI restriction sites using DNA Ligation Kit (TAKARA). The ORFs of zebrafish etf1b (ENSDARG00000043976 ...
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant Cas9 protein (TAKARA) and the sgRNA were incubated for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish rpl36 (ENSDARG00000100588) was amplified by RT-PCR and cloned into pCS2+ via EcoRI/XhoI restriction sites using DNA Ligation Kit (TAKARA). The ORF of zebrafish znf598 (ENSDARG00000014945 ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 U/ml RNase inhibitor (TAKARA), Complete Protease Inhibitor EDTA-free in nuclease-free water) ...
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bioRxiv - Molecular Biology 2023Quote: ... were subcloned in frame from their corresponding SMAUG1-HisTEV2xFLAG plasmids into pEGFP-N1 (Clontech) using XhoI and BamHI ...
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bioRxiv - Molecular Biology 2023Quote: ... 14-3-3γ CDS was amplified by PCR and subcloned into pCMV-mCherry (Clontech). Deletion and point mutations of SMAUG1 were created using Q5 site-directed mutagenesis kit (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: 5 ml TALON metal affinity resin (TaKaRa Bio) was equilibrated with five column volumes of native lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Lenti-X 293 cells (Takara Bio Inc.) were seeded and transfected with pSIH-H1 shRNA lentivectors (System Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was extracted from tissues using NucleoSpin RNA (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% Triton X-100) with 400U RNase inhibitor (Takara Bio Recombinant RNase Inhibitor ...
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bioRxiv - Neuroscience 2023Quote: ... 40U/mL of RNAse inhibitor (Takara: 2313B)] ...