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Citations for Takara Bio :
2401 - 2450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... with the One Step PrimeScript™ RT-PCR Kit (Takara Bio, RR064A). The following primers targeting a 244-base amplicon of the IAV M segment were used:
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 100ng/mL Doxycycline and 1µM Shield1 (Clontech Takara) for 15h to induce the expression of Dam-fused proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 100ng/mL Doxycycline and 1µM Shield1 (Clontech Takara) for 15h to induce the expression of Dam-fused proteins ...
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bioRxiv - Microbiology 2024Quote: ... XbaI (Takara Bio, Shiga, Japan) and SacI (Takara Bio) ...
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bioRxiv - Microbiology 2024Quote: ... One microgram of total RNA was used for reverse transcription with Revert Aid™ Reverse Transcriptase (TaKaRa, Tokyo, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Total RNA was isolated from larval zebrafish or liver /spleen /kidney tissues of adult zebrafish with Trizol reagent (TaKaRa, Tokyo, Japan) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... vectors using In-Fusion cloning (Takara). HA-RelA was codon-optimized and synthesized as dsDNA fragment (IDT ...
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bioRxiv - Microbiology 2024Quote: ... and all gene cloning operations were performed using an In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan). A conjugated pZJD29a vector [65] was employed to clone 500-bp upstream and downstream regions of the target gene ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng of RNA was used as input for cDNA synthesis using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio, San Jose, CA, USA). Library preparation was performed using the Nextera XT DNA Library Preparation Kit (Illumina) ...
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bioRxiv - Neuroscience 2024Quote: ... and the corresponding DNA amplicons were cloned into the mammalian expression vector pIRES2-EGFP (Clontech) using restriction enzymes listed in Table S5 ...
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bioRxiv - Molecular Biology 2024Quote: ... EYFP-Mito (EYFP fused to mitochondrial targeting sequence of cytochrome C oxidase subunit VIII) and EYFP-ER (EYFP fused to Calreticulin ER-targeting sequence) were from Clontech (Takara Bio, Kusatsu, Japan); Vector for CRISPR/Cas9-mediated knock-out of BCL-2 was plentiCTRSPRv2 (Addgene #52961 ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian” kit (Takara Bio USA, Cat. #634411). The protocol was started with 4-10 ng of total RNA ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus) (#RR42WR, Takara Bio) with a final forward and reverse primer concentrations of 1 µM and cDNA concentrations of 2.5 ng/µl ...
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bioRxiv - Plant Biology 2024Quote: ... coli (Takara, DH5α) using QIAGEN® Plasmid Midi Kit (QIAGEN ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested backbone and the two amplicons were joined by In-Fusion® HD Cloning Kit (Takara). The SFFV U3 in the pAS.SFFV.d2GFP.PRE plasmid was then exchanged for other U3 sequences by digesting the plasmid with XcmI and AscI (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... and anti-GFP (Takara, 632592) antibodies.
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bioRxiv - Biochemistry 2024Quote: ... Solubilized proteins in the supernatant were first purified using His60 Ni Superflow Resins (Takara Bio USA, 635660) and were eluted with 50 mM to 0.5 M gradient imidazole buffer containing 50 mM Na2HPO4 and NaH2PO4 ...
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bioRxiv - Biochemistry 2024Quote: ... PCR was performed with PrimeSTAR® GXL DNA Polymerase (Takara, R050A), PrimeSTAR GXL Buffer (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatant was incubated with TALON beads (Takara Clontech) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis and library preparations were performed by the Genomics Core using the SMARTv4 for ultra-low RNA input and Nextera XT kits (Takara). Sequencing was also performed on an Illumina NextSeq 2000 sequencing system with paired-end ...
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bioRxiv - Neuroscience 2024Quote: ... GRIK1-1a or GRIK1-2a cloned in pRK7 (in the presence or absence of rNeto1/rNeto2 cloned in IE-pRK8 as indicated) with EGFP expressing plasmid using Xfect (Clontech) according to manufacturer’s instructions (receptor ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatant was incubated with TALON beads (Takara Clontech) for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were barcoded using SMARTer Unique Dual Index Kit (Takara Bio USA, Inc). Five PCR cycles were run and PCR products were purified using AMPure XP reagent (Beckman Coulter Life Sciences ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v3 (Takara Bio USA, Inc) following the recommended protocol with minor adjustments ...
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bioRxiv - Synthetic Biology 2024Quote: ... and ptdTomato-C1 (Clontech), respectively ...
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bioRxiv - Synthetic Biology 2024Quote: ... prior to insertion in the cloning vectors pEGFP-C1 (Clontech) and ptdTomato-C1 (Clontech) ...
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bioRxiv - Developmental Biology 2024Quote: All DNA constructs used in this study were generated by using In-Fusion HD Cloning kit (Takara Bio Inc.). Plasmid mEmerald-N1 was a gift from Michael Davidson (Addgene plasmid #53976) ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted from pool of 30 embryos at 1 dpf using trizol reagent (Takara), chloroform (Sigma Cat ...
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bioRxiv - Developmental Biology 2024Quote: ... 100pg of sgRNA was injected along with 500 pg of spCAS9 protein (Takara). The F0 embryos were grown to adulthood and were then outcrossed with wild type to give rise to F1 animals ...
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bioRxiv - Developmental Biology 2024Quote: ... Full-length cDNA fragment of human MAK was amplified by PCR using human retinal cDNA (Clontech) as a template and subcloned into the pCAGGSII-2xHA vector (62) ...
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bioRxiv - Developmental Biology 2024Quote: ... MS2-orf147 hybrid RNA expressing in pPRP1 vector and identified PPRs expressing in prey vector (pGADT7) All three components were then co-transformed into yeast host (Saccharomyces cerevisiae strain Y187 (Takara) at equimolar concentrations in DDO/X/A [SD/–Leu/–Trp/X-a-Gal/AbA] media (containing kanamycin to prevent bacterial contamination ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA (2 μg) was reverse-transcribed into cDNA with random hexamers using the PrimeScript II reagent (TaKaRa). Human cDNA was purchased from Clontech ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into the PCS2+ vector (5x In-Fusion HD Enzemy Premix, Takara, 639649). The primers for cloning were as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... The cDNAs were used for PCR with rTaq polymerase (TaKaRa). The primer sequences used for amplification are listed in Table S1.
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bioRxiv - Developmental Biology 2024Quote: ... 2 µl of cDNA was used together with specific forward and reverse primers for primary miR-43093 (2 µM; Supp. Table 1) and 5 µl of SYBR® Premix-Ex-Taq (Tli RNase H Plus, Takara) in a 10 µl reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... according to manufacturer’s directions for the SMART-seq v4 Ultra Low Input RNA Kit (Takara, 634891) at a 1/8th reaction volume and using the Mantis (Formulatrix ...
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bioRxiv - Developmental Biology 2024Quote: ... To mark epithelial cells anti-Ecad (M108, Takara Bio), and anti-Sox9 (AB5535 ...
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bioRxiv - Neuroscience 2024Quote: ... Low-input ribosome depleted (SMART-seq; Takara Bio; library size: 200-500bp) library prep was used for downstream paired-end 2x150bp RNA sequencing at a 50 million read depth using NovaSEQ 6000 (Illumina) ...
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bioRxiv - Neuroscience 2024Quote: ... Then the RNA-seq library was prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit (TaKaRa Bio, USA). This was performed in Shpokayte et ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Gorilla CD4 alleles and ancestral CD4s were commercially synthesized (IDT GeneBlocks) and gateway cloned into the pLPCX retroviral packaging vector (Clontech). Mutant versions of human and gorilla CD4 were constructed by standard site-directed mutagenesis methods using overlapping PCR primers encoding the modification ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-dsRed (cross-reacts with TdTomato, 1:1,000, Clontech, Cat#632496), mouse anti-FAT36,7 (1:200) ...
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bioRxiv - Neuroscience 2024Quote: ... 7 million Lenti-X HEK-293T cells (Takara Bio, 632180) were seeded in 9 mL DMEM ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from flash-frozen cells was processed using NucleoMag (744350.1, Takara) Magnetic bead-based RNA isolation ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral titer was then determined using the Lenti-X p24 rapid titer kit (Takara Cat #632200). The Tau-RD-P301S-K18 FRET Biosensor CRL-3275 were exposed to active recombinant P301S-MAPT fibrils (ab246003 ...
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bioRxiv - Cell Biology 2024Quote: ... we harvested and concentrated the lentiviral particles using the LentiX concentrator (Takara #631232) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The mutations were introduced by site-directed mutagenesis via PCR (CloneAmp Hifi, Takara). All mutations were assessed by complete sequencing of the gene (Eurofins Genomics) ...
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bioRxiv - Neuroscience 2024Quote: ... DLK and DRP1 were N-terminally tagged into a GST-containing backbone using In-fusion cloning (Takara Bio. 638945). Cyto-mAPPLE plasmid was a gift from Michael E ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared from <=2.5ng input RNA using ¼ volume SmartSeqv4 technology (Takara Bio) and sequenced on NextSeq High Output flow cells ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 6 ml Lenti-X Concentrator (Takara Bio; 631231), mixed thoroughly ...
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bioRxiv - Cell Biology 2024Quote: ... reached 0.6 and IPTG was added (TaKaRa Bio, TKR9030) to a final concentration of 1 mM ...