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Citations for Takara Bio :
2351 - 2400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... and transferred to a 2 mL gravity column (Takara). The end-cap of the column was removed to drain the buffer and 500 uL of wash buffer was added to wash the resin once more ...
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bioRxiv - Synthetic Biology 2024Quote: ... TALON Metal Affinity Resin (Takara) protocols were used ...
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bioRxiv - Synthetic Biology 2024Quote: ... For conditions with the addition of BB homodimerizer (AP20187)(Takara Bio, San Jose, USA), 1μL of 0.5mM BB was diluted in 999μL PBS (HI30 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were collected and AAV6 capsids were isolated using the AAVPro Purification Kit (All Serotypes, Takara Bio, San Jose, USA), as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... sites flanking gene-specific nucleotides and cloned first into an entry vector pDONR207 and then into destination yeast two-hybrid vectors pGADT7 or pGBKT7 (Clontech, Takara Bio USA) using Gateway cloning kit (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... sites flanking gene-specific nucleotides and cloned first into an entry vector pDONR207 and then into destination yeast two-hybrid vectors pGADT7 or pGBKT7 (Clontech, Takara Bio USA) using Gateway cloning kit (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments were amplified using PCR using PrimeSTAR® Max DNA Polymerase (Takara Biomedical Technology (Beijing) Co. ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α (Takara Bio. Tech.) was used for molecular cloning to construct plasmids ...
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bioRxiv - Synthetic Biology 2024Quote: ... Lenti-X Concentrator (Takara Bio, 631232) was added in a 3 parts media ...
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bioRxiv - Synthetic Biology 2024Quote: Initial testing used four DNA polymerases: LA Taq Hot Start (TaKaRa, Shiga Japan), Herculase II (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated from 500ng of RNA using PrimeScript RT Master Mix (Takara, Cat. # RR036B). The cDNA was diluted 1:20 and 1µl of the diluted cDNA was used for qPCR analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Viral supernatants were harvested at 48 and 72 hours post transfection and concentrated with lenti-X concentrator solution (Clontech). TRE-MYC HCECs were infected in triplicate at a low MOI (0.2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Expression of cDNAs was induced with 100 ng/mL doxycycline (Clontech/Takara). Site-directed mutagenesis was used to generate the citrate binding mutant SLC25A1 ...
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bioRxiv - Cancer Biology 2024Quote: ... it was concentrated 100-fold from cell culture medium using Lenti-X™ Concentrator (Takara, #631231) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was further diluted for LDH detection according to the product manual (MK401, Takara). Briefly ...
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bioRxiv - Systems Biology 2024Quote: ... by transfection and concentrated using Lenti-X concentrator (Takara Bio, Cat# 631231). We transduced NPCs with the concentrated lentiviruses and selected the transduced NPCs using 4ug/ml puromycin until the BFP+ cells reached greater than 90% ...
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bioRxiv - Cancer Biology 2024Quote: ... Expression of cDNAs was induced with 100 ng/mL doxycycline (Clontech/Takara). Site-directed mutagenesis was used to generate the citrate binding mutant SLC25A1 ...
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bioRxiv - Cell Biology 2024Quote: ... The PrimeScript RT Master Mix Kit (TaKaRa) was used to generate cDNA from the extracted RNA ...
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bioRxiv - Systems Biology 2024Quote: ... Lentiviruses carrying sgRNAs were produced in Lenti-X 293T cells (Takara Bio, Cat#632180) by transfection and concentrated using Lenti-X concentrator (Takara Bio ...
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bioRxiv - Plant Biology 2024Quote: ... Purification of the His6-tagged protein was achieved by TALON® spin columns (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cDNA was reverse transcribed by using a PrimeScript RT Reagent Kit Perfect Real Time (TaKaRa Bio Inc.). Real-time PCR was performed using a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Gorilla CD4 alleles and ancestral CD4s were commercially synthesized (IDT GeneBlocks) and gateway cloned into the pLPCX retroviral packaging vector (Clontech). Mutant versions of human and gorilla CD4 were constructed by standard site-directed mutagenesis methods using overlapping PCR primers encoding the modification ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-dsRed (cross-reacts with TdTomato, 1:1,000, Clontech, Cat#632496), mouse anti-FAT36,7 (1:200) ...
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bioRxiv - Neuroscience 2024Quote: ... Low-input ribosome depleted (SMART-seq; Takara Bio; library size: 200-500bp) library prep was used for downstream paired-end 2x150bp RNA sequencing at a 50 million read depth using NovaSEQ 6000 (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: ... The quantitative real-time-polymerase chain reaction (qPCR) was conducted with SYBR Premix Ex Taq™ II (Takara, Dalian, China), and performed on on a QuantStudio™ 7 Flex Real□ Time PCR System ...
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bioRxiv - Molecular Biology 2024Quote: ... using an In-Fusion cloning kit (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... synthesized 5’ and 3’ fragments of the target RNA were 32P-radiolabeled at the 5’ end using T4 polynucleotide kinase (Takara). After gel purification ...
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bioRxiv - Immunology 2024Quote: ... total RNA (0.5 ng) was added to reaction buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), and reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Genomics 2024Quote: ... 152 ∗106 GPRTG cells were seeded into a 2-STACK culture chamber (Cornig) and transfected 24 h later with 238 µg transfer plasmid using the CalPhos Mammalian Transfection Kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Then the RNA-seq library was prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit (TaKaRa Bio, USA). This was performed in Shpokayte et ...
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bioRxiv - Neuroscience 2024Quote: ... or In-Fusion HD Cloning Kit (TaKaRa). The GFP-fused full-length NCAN-expressing plasmid was constructed by inserting the fragment encoding GFP amplified from the pCAG-GFP vector into the central region of NCAN (corresponding to Val641 to Leu644) ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibodies (mouse anti-GFP; Molecular Probes, Cat # A11120; diluted 1:1,000 and rabbit anti-dsRed; Clontech, Cat # 632496; diluted 1:1,000) were added to fresh blocking buffer and incubated with the samples overnight at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast two hybrid assays were carried out using Gal4 based system (Clontech) by introducing plasmids harboring gene of interest in yeast strains AH109 and Y187 ...
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bioRxiv - Plant Biology 2024Quote: ... Bio-RAD CFX96 system was applied to perform qRT-PCR with TB Green Premix Ex Taq (Takara). Relative expression level calculation method was follow as described.
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bioRxiv - Plant Biology 2024Quote: ... Rwt4Ca and its variant proteins were cloned into the prey vector pGADT7 (Takara, #630442) while AvrPwt4 and VirPwt4 were cloned into the bait vector pGBKT7 (Takara ...
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bioRxiv - Microbiology 2024Quote: ... to make each ORF compatible for In-Fusion cloning (Takara Bio). Sequences were assembled into pcDNA3.1 vector encoding VC155 or VN173 on the N- or C-terminus ...
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bioRxiv - Microbiology 2024Quote: ... The rNiV TC-tr minigenome encoding VC155 was constructed by subcloning the VC155 sequence from the pcDNA3.1 VC155-2xLinker-NiV-M construct and assembling it into restriction-digested rNiV TC-tr minigenome by In-Fusion cloning (Takara Bio) approaches ...
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bioRxiv - Plant Biology 2024Quote: Pc12 and truncated Pc12 were cloned into pGBKT7 vector (Takara Bio, Japan), while Rab13-2 and Rab13-2 mutants were inserted into pGADT7 vector ...
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bioRxiv - Molecular Biology 2024Quote: ... The precipitated RNAs were reverse transcribed by PrimeScript II reverse transcriptase (Takara Bio) with SP6 primer ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNAs were reverse transcribed using PrimeScript II reverse transcriptase (Takara Bio) with oligo-dT and random primers ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycopeptides were treated with PNGase F (Takara Bio, Shiga, Japan) in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using a PrimeScript reverse transcriptase reagent kit (Takara, Japan). mRNA expression was examined by quantitative real-time PCR using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... 7 million Lenti-X HEK-293T cells (Takara Bio, 632180) were seeded in 9 mL DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... DLK and DRP1 were N-terminally tagged into a GST-containing backbone using In-fusion cloning (Takara Bio. 638945). Cyto-mAPPLE plasmid was a gift from Michael E ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared from <=2.5ng input RNA using ¼ volume SmartSeqv4 technology (Takara Bio) and sequenced on NextSeq High Output flow cells ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from flash-frozen cells was processed using NucleoMag (744350.1, Takara) Magnetic bead-based RNA isolation ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral titer was then determined using the Lenti-X p24 rapid titer kit (Takara Cat #632200). The Tau-RD-P301S-K18 FRET Biosensor CRL-3275 were exposed to active recombinant P301S-MAPT fibrils (ab246003 ...
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bioRxiv - Cell Biology 2024Quote: ... we harvested and concentrated the lentiviral particles using the LentiX concentrator (Takara #631232) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The mutations were introduced by site-directed mutagenesis via PCR (CloneAmp Hifi, Takara). All mutations were assessed by complete sequencing of the gene (Eurofins Genomics) ...
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bioRxiv - Cell Biology 2024Quote: ... coli strain BL21-competent cells (TaKaRa Bio, TKR9126). Colonies were inoculated into LB broth for starter cultures incubated at 37°C overnight ...