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Citations for Takara Bio :
2301 - 2350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Gastruloids were generated as described before (11,14) with the following adaptations: N2B27 medium (Takara) was passed through a .22 µm filter before use and 600 cells were used for aggregation instead of 300.
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bioRxiv - Developmental Biology 2024Quote: All DNA constructs used in this study were generated by using In-Fusion HD Cloning kit (Takara Bio Inc.). Plasmid mEmerald-N1 was a gift from Michael Davidson (Addgene plasmid #53976) ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted from pool of 30 embryos at 1 dpf using trizol reagent (Takara), chloroform (Sigma Cat ...
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bioRxiv - Developmental Biology 2024Quote: ... 100pg of sgRNA was injected along with 500 pg of spCAS9 protein (Takara). The F0 embryos were grown to adulthood and were then outcrossed with wild type to give rise to F1 animals ...
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bioRxiv - Developmental Biology 2024Quote: ... Full-length cDNA fragment of human MAK was amplified by PCR using human retinal cDNA (Clontech) as a template and subcloned into the pCAGGSII-2xHA vector (62) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and transformed into Stellar competent cells (Takara, Cat. No. 636763). For cell entry validation mutants ...
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bioRxiv - Genetics 2024Quote: ... and 1 U TaKaRa LA Taq (Takara Bio Inc., Kyoto, Japan) was prepared ...
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bioRxiv - Cell Biology 2024Quote: ... with 10% FBS (Clontech) and 1% penicillin- streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by infusion cloning (Takara, 638911) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5-1.0 μg total RNA was used for cDNA synthesis with 5x PrimeScript RT Master Mix (Takara, RR036A-1). Quantitative real-time PCR was carried out using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: The plasmids used for transient overexpression were constructed using the pCMV-FLAG/Myc vector (Clontech). Point and deletion mutants were engineered utilizing the KOD-Plus-Mutagenesis Kit (TOYOBO ...
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bioRxiv - Genetics 2024Quote: ... Esrp1 cDNA was cloned into the pcDNA3.1 backbone containing a CMV promoter and SV40 polyA tailing sequence for expression in mammalian cells using the In-Fusion HD Cloning Kit (Clontech) to generate the pcDNA3.1-esrp1-mCherry plasmid ...
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bioRxiv - Genetics 2024Quote: ... cDNA was generated from 1μg of total RNA using the PrimeScriptTM RT reagent kit (TaKaRa, Shiga, Japan) for the quantitation of rRNA by quantitative real-time PCR ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from the leaves using the Acid Phenol Guanidium Chloroform method (Chomczynski and Sacchi 2006) and was then treated with 10 U of DNase I (Takara Bio Inc., Kyoto, Japan). poly(A)+ RNA was separated and purified from total RNA using an oligo(dT ...
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bioRxiv - Genetics 2024Quote: ... was cloned into the linearized DNA template vector (Takara Bio USA). The plasmid vectors were purified by a QIAprep spin miniprep kit (QIAGEN) ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified PCR fragment was ligated into pMD20-T vector (Takara Bio). The Cas9 plasmid was used as a template for in vitro transcription using mMESSAGE mMACHINE T7 Ultra Kit ...
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bioRxiv - Cell Biology 2024Quote: ... HEK-293 cells were transfected with the expression vector and secreted protein was purified by affinity chromatography (His60 resin, Clontech) and size exclusion chromatography (26/60 Superdex 200 HiLoad (GE) ...
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bioRxiv - Developmental Biology 2024Quote: ... and used in quantitative real time reverse transcriptase PCR (qRT-PCR) with Takara SYBR Premix Ex Taq (Tli RNaseH Plus) (Takara Bio USA Inc, Mountain View, CA, USA) and LC480 (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... The RT-qPCR amplifications were performed using 2× SYBY Premix Ex TapTM II (Takara, China) as described previously ...
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bioRxiv - Developmental Biology 2024Quote: ... Blastocysts were then transferred to embryonic stem (ES) cell culture medium (Cellartis 3i mES/iPSC medium, Takara Bio, catalog # 1181722446) in a cell culture dish covered with mouse embryonic fibroblast (MEF ...
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bioRxiv - Developmental Biology 2024Quote: ... 1:2000 monoclonal anti-mCherry (Takara/Clontech #632543), 1:1000 polyclonal anti-mCherry (Chromotek pabr1 ...
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bioRxiv - Developmental Biology 2024Quote: ... The fragment was then inserted in the pCAGEN-IRES-tomato with the In-Fusion® HD Cloning Kit (639649, Takara bio) according to manufacturer recommendations to generate pCAGEN-ATP1A3-WT-IRES-Tomato (named ATP1A3-WT) ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s specifications and SMART-Seq v4 Ultra Low Input RNA Kit (TAKARA) was used to synthesize cDNA from messenger RNA ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed using TB Green Premix Ex Taq (Takara). The ΔCt method using Gapdh as a reference gene was used to analyse relative expression and the 2−ΔΔCt (Livak ...
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bioRxiv - Developmental Biology 2024Quote: ... using the same paired read adapter oligonucleotides described by 99 were prepared from the ChIP and Input DNAs on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step ...
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bioRxiv - Plant Biology 2024Quote: ... and a 500-ng RNA was used for the first-strand cDNA synthesis using PrimeScript™ 1st strand cDNA Synthesis Kit (6110A; TaKaRa, San Jose, California, USA) with oligo(dT ...
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bioRxiv - Plant Biology 2024Quote: ... and cloned into pMDC32 using In-Fusion HD cloning (TaKaRa, San Jose, California, USA) for sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... under the control of the CaMV35S promoter in the binary vector pBI121 (Clontech, USA), was transferred into Agrobacterium tumefaciens strain GV3101 and used to transform tobacco plants by leaf disc method (Horsch et al. ...
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bioRxiv - Plant Biology 2024Quote: ... The potato ST-LS1 IV intron (Eckes et al., 1986) was amplified (Infusion, Takara Bio) using M.SssIN_IV forward and M.SssIC_IV reverse primers (Sup Table 6 ...
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bioRxiv - Neuroscience 2024Quote: ... Confluent HEK293 cells were transfected using a mixture of 50 μl of Opti-MEM I containing a total of 0.9 μg of cDNA constructs encoding hGluN1/hGluN2 subunits and GFP (for identification of successfully transfected cells; pQBI 25, Takara) in a 1:1:1 ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR (qPCR) reactions were performed in triplicate using SYBR master mix (Takara), following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... rat AT1 and RlucII were subcloned into the pLVSIN-CMV Neo vector (Takara Bio, Japan). Next ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using the ThruPLEX DNA-Seq Kit and DNA Unique Dual Index Kit (Takara) according to the manufacturer’s manual ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Target sequences were amplified in a first PCR using gene-specific primers (F1 and R1) and EmeraldAmp GT PCR Master Mix (TaKaRa RR320A). The PCR conditions were ...
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bioRxiv - Plant Biology 2024Quote: The full-length FLP1 cDNA in pENTR/D-TOPO was cloned into pDEST_GBKT7 vector85 by Gateway reaction and transformed into Y2HGold yeast strain (Takara Bio). The resultant yeast was cultured in synthetic defined medium (–W) ...
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bioRxiv - Immunology 2024Quote: ... SMART-Seq HT Kit (Takara) was used to construct cDNA and libraries ...
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bioRxiv - Plant Biology 2024Quote: RT‒PCR splicing analysis was carried out with Ex-Taq polymerase (Takara, Japan) for a total of 25-28 cycles ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from RNA (approximately 2 μg) with oligo(dT) primers according to the instructions of the PrimeScript First Strand cDNA Synthesis Kit (Takara).
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bioRxiv - Plant Biology 2024Quote: ... with the SYBR Premix Ex-Taq Kit (Takara). EF-1α (AT1G18070 ...
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bioRxiv - Neuroscience 2024Quote: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); guinea pig anti-DsRed (1:500 ...
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bioRxiv - Genetics 2024Quote: ... Genomic regions were subsequently amplified using EpiTaq HS (Takara Bio, Shiga, Japan) and cloned into the pGEM-T Easy vector (Promega ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from tissues using RNAiso Plus (Takara). The extracted RNA was quantified by Nanodrop and 500 ng of it was converted into cDNA using a PrimeScript™ RT reagent Kit (Takara) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA fragments of approximately 1.5 kb upstream and downstream of LBDG_21500 required for homologous recombination were amplified via polymerase chain reaction (PCR; PrimeSTAR Max DNA polymerase, Takara, Shiga, Japan) using L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were constructed via site-directed mutagenesis using the PrimeSTAR Mutagenesis Basal Kit (Takara) with p21500_complement as the template ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and upstream fragment) were connected using the In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, Shiga, Japan) to construct the LBDG_21500-disrupting plasmid (Table S9).
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bioRxiv - Molecular Biology 2024Quote: ... Yeast strain AH109 (Clontech, Takara Bio USA) was used for yeast two-hybrid interaction studies as a tester strain ...
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bioRxiv - Microbiology 2024Quote: The 500 bp of DNA upstream and downstream of a target gene were amplified by PCR (Primestar Max DNA Polymerase, Takara). The two 500 bp fragments were then fused by overlapping PCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... cultures were supplemented with 0.5 μg·ml−1 anhydrotetracycline (aTc) (Clontech, Mountain View, CA) and 1 mM neuraminic acid (Neu5Ac ...
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bioRxiv - Synthetic Biology 2024Quote: ... cultures were induced with 0.5 μg·ml−1 anhydrotetracycline (aTc) (Clontech, Mountain View, CA). When experimental conditions required it ...
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bioRxiv - Synthetic Biology 2024Quote: ... 500 uL of Elution Buffer (Takara) was then added to the column to elute the TdT into a clean 1.5 mL tube ...