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Citations for Takara Bio :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The protein was purified to homogeneity using a combination of TALON Metal Affinity Resin (Takara) and gel filtration chromatography ...
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bioRxiv - Plant Biology 2024Quote: ... using the SYBR Premix Ex TaqTM (TaKaRa, Japan). Gene expression was normalized using UBQ10 and ACT2 as internal standards ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid analysis was performed using the MatchMaker GAL4 Two-Hybrid System 3 (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... together with three DNA markers (λ-Hind III digest; Takara Cat ...
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bioRxiv - Genomics 2024Quote: The Iso-Seq libraries were constructed from 1 µg of total RNA per sample and full-length cDNA were then generated using the SMARTer PCR cDNA synthesis kit (Takara Bio Inc, 639506). The libraries were sequenced on the Sequel Instrument v1 ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA encoding ZP3 was restored from the mouse ovarian tissue by RT-PCR using PrimeScript RT Reagent Kit (Takara, RR037). Two PCR amplicons including the 5′ region of the TECTA-ZP and the transmembrane domain (TMD ...
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bioRxiv - Developmental Biology 2024Quote: ... 500 ng of RNA template was subjected to in vitro reverse transcription using PrimeScript RT Reagent Kit (Takara, RR037). After elution with 40 ul of DNase/RNAse free water ...
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bioRxiv - Genetics 2024Quote: ... purified by phenol chloroform extraction and then reverse transcribed with a PrimeScript™ RT Reagent Kit (TAKARA, Dalian, China) according to the manufacturers’ protocol.
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bioRxiv - Biophysics 2024Quote: ... Lentivirus was concentrated 15-fold using LentiX concentrator reagent (Takara), resuspended in PBS ...
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bioRxiv - Microbiology 2024Quote: ... and assembled by In-Fusion (TaKaRa, Japan). After purification with the NucleoSpin Plasmid kit (Macherey Nagel ...
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bioRxiv - Immunology 2024Quote: ... RNAse Inhibitor (Takara)) in a 96-well V-bottom PCR plate (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... DNA adapters were ligated to MmeI-digested DNA fragments by T4 DNA ligase (Takara), and the ligation products were purified on magnetic beads ...
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bioRxiv - Physiology 2024Quote: ... SYBR green (TaKaRa) and ABI7500 system (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... The resulting double-stranded cDNA’s were further processed to DNA sequencing libraries using ThruPLEX DNA-seq 12S Kit (R400428, Clontech Laboratories). Libraries were size-selected by gel purification for an average size of 350bp ...
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bioRxiv - Developmental Biology 2024Quote: ... with eGFP (Clontech) and mKate2 (Evrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR amplification was first performed with PrimeSTAR GXL Premix (Takara Bio, R051A) to attach Nextera-style adapter sequences ...
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bioRxiv - Plant Biology 2024Quote: ... or the SMART-Seq mRNA LP Kit (Takara Bio USA) (for pre-parasitic J2 library generation) ...
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bioRxiv - Plant Biology 2024Quote: ... using the In-Fusion HD Cloning Kit (Clontech) kit ...
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bioRxiv - Plant Biology 2024Quote: ... the 5 μl samples were used as input into the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA) (for parasitic J3 library generation ...
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bioRxiv - Synthetic Biology 2024Quote: ... has been acquired from Takara Bio.
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bioRxiv - Synthetic Biology 2024Quote: ... and IV from M13cp following PCR amplification and ligation using the In-Fusion Snap Assembly Master Mix (Takara Bio, 638944). The resulting helper plasmids were transformed into DH5α competent cells (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... restriction enzymes and T4 DNA ligase from NEB and PCRs were done using the PrimeSTAR Max DNA polymerase from Takara Bio ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... lentiviruses were produced in Lenti-X 293T cells (Takara Bio, Cat#632180) and lentivirus-containing supernatants were concentrated using Lenti-X concentrator (Takara Bio ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... and lentivirus-containing supernatants were concentrated using Lenti-X concentrator (Takara Bio, Cat#631231). NPCs were transduced and selected using 4ug/ml puromycin (Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... Total RNA was isolated from cells using TRIzol Reagent following the instructions and genomic DNA was removed using DNase I (TaKara). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs efficiency was assessed in vitro using the sgRNA In vitro Transcription and Screening kit (Clontech, 631439) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... using the PrimeScript RT Master Mix (Takara). We then amplified cDNA in Power SYBR (Thermo ...
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bioRxiv - Plant Biology 2024Quote: ... The CtBOT6 DNA fragment and the amplified vector were then merged using the In-Fusion HD Cloning Kit (TaKaRa). Ct4 transformed with the pBin-GFP-hph-CtBOT6 vector express CtBOT6 under the TrpC promoter control ...
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bioRxiv - Plant Biology 2024Quote: ... amplified promoter region fragments are inserted with KpnI and XhoI site in pAT006-Fluc-iG5_Rluc-iFAD2_F vector using In-Fusion HD Cloning Kit (Takara). Amplified BOT6 fragment was inserted with pGWB402 using pENTR™ Directional TOPO® Cloning Kits (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: Lenti-X 293T cells (Takara, Cat # 632180) were transfected with each CAR expression plasmid and Mirus Bio™ TransIT™-Lenti Transfection Reagent (Cat # MIR6600) ...
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bioRxiv - Cancer Biology 2024Quote: ... and then lentivirus was harvested and concentrated using Lenti-X Concentrator (Takara Bio, 631232).
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bioRxiv - Cell Biology 2024Quote: ... libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634411). Single-end sequencing was performed on the Ilumina NovaSeq platform with a sequencing depth of 80 million reads and a read length of 100 bp.
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bioRxiv - Cancer Biology 2024Quote: ... sterilized and titre measured (Go-Stix, Takara). The cell lines CACO2 ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Linearized pMK1334 vector was purified using NucleoSpin Gel and PCR clean-up kit (Takara Bio, Cat#740609.50). Top and bottom oligos of non-targeting and targeting sgRNAs were annealed ...
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bioRxiv - Biophysics 2024Quote: ... The cell lysate was then clarified with centrifugation and the supernatant was collected and loaded onto a gravity column manually loaded with TALON Metal Affinity Resin (Takara), washed 5 times with 10 column volumes (CV ...
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bioRxiv - Zoology 2024Quote: ... The complementary DNA (cDNA) synthesis was achieved by using ReverTra Ace-α-(Toyobo) with random primer (Takara Bio). Subsequently ...
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bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2024Quote: ... were cloned into pGBKT7-GW and pGADT7-GW (Takara Bio) vectors through Gateway cloning ...
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bioRxiv - Plant Biology 2024Quote: ... or Prime STAR HS DNA Polymerase (TaKaRa) with standard protocols.
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bioRxiv - Plant Biology 2024Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara). Quantitative real-time PCR was performed at 60°C using Takyon No ROX SYBR 2X MasterMix blue dTTP (Eurogentec ...
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bioRxiv - Plant Biology 2024Quote: Single or multiple DNA fragments were cloned into fungal transformation vector using In-Fusion HD Cloning Kit (Clontech, USA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... Bait and prey constructs were transformed into the haploid yeast strains Y2HGold and Y187 (Matchmaker Gold Yeast Two-Hybrid System, Clontech), respectively ...
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bioRxiv - Plant Biology 2024Quote: ... We then ran a western blot using the GFP antibody (Takara living colors EGFP monoclonal antibody JL-8 ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA library was prepared using the SMART-Seq HT kit (Clontech). Illumina indexes were added to cDNA using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: ... the C-terminal twin strep tag was removed by incubating with the 3C protease (Takara Bio, Japan; 1.5 unit/50 mg protein) and ran on a size-exclusion column ...
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bioRxiv - Immunology 2024Quote: ... reverse transcription and PCR steps were directly performed after sorting according to the SMART-Seq® v4 Ultra® Low Input RNA Kit protocol (Takara Bio, USA). Amplified complementary DNA were transferred to the Benaroya Research Institute (Seattle ...
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bioRxiv - Immunology 2024Quote: ... of 25 μg/mL Retronectin (Takara) in PBS and incubated at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... HEK 293T cells were sourced from the American Type Culture Collection (ATCC) and GP2-293 from Takara Bio ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR-amplified enhancer-containing fragments were tandemly inserted into the upstream region of the original promoter sequence by In-Fusion cloning (TAKARA BIO, Shiga, Japan). This process is summarized in Figure S3 ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was processed with dpn I (Takara Bio Inc.) to digest the template plasmid and then transformed into E ...