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Citations for Takara Bio :
1 - 50 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... TaqMan-qPCR was performed using the Premix Ex Taq (Probe qPCR) Kit (TaKaRa, cat# RR390L) in an StepOne Plus Real-Time PCR system (Applied Biosystem ...
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bioRxiv - Microbiology 2024Quote: ... using PrimeScript RT (TAKARA) and random hexamers ...
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bioRxiv - Microbiology 2024Quote: ... GFP4 -and GFP7 expressing vector using In-Fusion cloning kit (Takara) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Cloning to vectors was performed with In-Fusion cloning (Takara) kit according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... cDNA was subsequently synthesized using Reverse Transcriptase M-MLV (TakaRa) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... His-tagged CD45RO was purified by TALON affinity resin (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and purified using the NucleoBond Xtra Midi endotoxin-free midi-prep kit (Takara Bio). For immunocytokines ...
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bioRxiv - Immunology 2024Quote: ... Plasmid sequences confirmed by Sanger sequencing (Quintara Biosciences) were transformed into Stellar Competent Cells (Takara Bio) and purified using the NucleoBond Xtra Midi endotoxin-free midi-prep kit (Takara Bio) ...
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bioRxiv - Cancer Biology 2024Quote: ... and first-strand cDNA was prepared using an AMV RNA PCR kit (TaKaRa) from 1 mg of total RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using Smarter Stranded Total RNA-Seq Kit - Pico Input Mammalian (Takara Bio, #635005). For committed cells ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolated RNA was reverse transcribed into cDNA using the PrimeScript™ RT Master Mix (Takara: RR036B) according to the the protocol provided by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... then concentrated using Retro-X concentrator overnight (Takara Bio #631455). Untreated tissue culture plates were coated with 25mg/ml Retronectin (Takara Bio #T100B ...
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bioRxiv - Cancer Biology 2024Quote: ... Untreated tissue culture plates were coated with 25mg/ml Retronectin (Takara Bio #T100B) overnight at 4°C to be used for retroviral transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... Secreted SEAP were detected using the Great EscAPe™ SEAP Chemiluminescence Kit 2.0 (Clontech#631738) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... MSC and HUVEC were procured from TAKARA (Shiga, Japan). MSCs were cultivated in Mesenchymal Stem Cell Growth Medium 2 (PromoCell ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed in triplicates with the SYBR Premix Taq II master mix (Takara) and specific primers on the LightCycler 96 (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... and RNA was extracted using Takara’s Nucleospin RNA Plus kit (Takara, 740984.50). RNA quantity and purity were assessed by Nanodrop 1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... three primer sets were designed with overlapping sections,100ng of Genomic DNA was used per PCR reaction with primeSTAR GXL DNA polymerase (Takara Bio) using a touchdown PCR for the first 10 cycles from 72 to 60 followed by 35-40 cycles at the proper annealing temperature (Tm −2°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Equimolar amounts of PCR products were mixed and a PCR was made with a primeSTAR GXL DNA polymerase (Takara Bio) without primers for the first 10 cycles using the following thermocycler conditions (95°C 3min ...
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bioRxiv - Cancer Biology 2024Quote: ... The BAC clone was purified using the NucleoBond Xtra BAC kit (Takara Bio). Alexa Fluor 488-labeled probe was generated through Nick translation system using the Chromatide Alexa Fluor 488-5-dUTP kit (Invitrogen) ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... or N2B27 medium (Takara, Y40002) supplemented with 1x Pen/Strep (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and concentrated with Lenti-XTM Concentrator (Takara, #631232). Media was replaced and on day 5 ...
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bioRxiv - Cancer Biology 2024Quote: ... U6 primers (TAKARA) were used to normalize miRNA expression via the 2-ΔΔCq method (ΔCq = Cqtarget − Cqrereference).
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bioRxiv - Cancer Biology 2024Quote: ... Shield1 (TaKaRa, Cat# 632189). The metabolites used in this study are as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... The sgRNA sequences were amplified using Taq polymerase (Takara Bio, Inc.) and adapted for sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Library concentration was estimated with the Library Quantification kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Tick DNA was extracted using the MightyPrep reagent for DNA Kit (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... GFP (Clontech), PABPC1 (Santa Cruz ...
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bioRxiv - Microbiology 2024Quote: ... expressing GFP under the control of the hCMV immediate-early promoter were obtained from Clontech. The construction of plasmids expressing vhs from HSV1 strain17 as vhs-GFP and untagged pcvhs have been described previously (13 ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were then reverse transcribed employing a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan). Nested PCR conditions were as follows ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: Capturem Co-IP kit was procured from TaKaRa (Japan). The Neuro-2a cells were transfected with pEGFP-PKM2 and cells were infected with JEV 24 hours post-transfection ...
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bioRxiv - Immunology 2024Quote: ... Virus was plated on non-tissue culture treated 24-well plates precoated with retronectin (TaKaRa) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Viruses were extracted and purified by using an AAV extraction kit (Takara).
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bioRxiv - Cancer Biology 2024Quote: ... Tet System Approved FBS (#631101, Takara) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... Signals were detected with ECL western blotting substrate (TaKaRa) and image was acquired using Azure Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Real time PCR was set up using TB Green Premix Ex Taq II (Tli RNase H Plus) (TakaRa) in a Quantstudio5 machine ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was made using 500ng RNA with PrimeScript RT Reagent Kit (TaKaRa). Real time PCR was set up using TB Green Premix Ex Taq II (Tli RNase H Plus ...
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bioRxiv - Molecular Biology 2024Quote: ... After 2 hrs 1µl of Reverse Transcriptase (PrimeScript RT, Takara) was added and reverse transcription was performed at 420C for 1hr followed by inactivation at 700C for 30 mins ...
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bioRxiv - Cancer Biology 2024Quote: ... The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Microbiology 2024Quote: ... and cultured in an overlay medium: MEM containing 0.8% SeaKem ME agarose (Takara, Kusatsu, Japan) and 4% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were then blocked in 5% Skim milk powder (Fujifilm, Cat# 190-12865) mixed with Phosphate buffered saline with tween® (PBS-T) (Takara, Cat# T9183). The chosen primary antibody was then incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... the annealed oligos were ligated into the digested lentiCRISPRv2 backbone using the DNA ligation kit (Mighty Mix) (Takara, Cat# 6023). Afterward ...
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bioRxiv - Molecular Biology 2024Quote: IgG detector solution V2 (1:2000 dilution, Takara, Cat# T7122A-1).
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bioRxiv - Cancer Biology 2024Quote: ... and 2 µl of lysate was used for PCR using SapphireAMP (Takara, RR350) and primers specific for each gene ...
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bioRxiv - Molecular Biology 2024Quote: The human embryonic kidney (HEK) 293T cell line was purchased from Takara (Cat# 632180) and cultured in a humidified 95% air / 5% CO2 incubator at 37°C in a standard Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... the media was replaced with NDiff227 (Takara) supplemented with 1 μM PD325901 (Stemgent) ...
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bioRxiv - Cell Biology 2024Quote: ... and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara) as per the instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was purified from at least 100 million cells of each sample using NucleoSpin Blood XL (Takara) or QIAamp DNA Blood Midi Kit (QIAGEN) ...
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bioRxiv - Microbiology 2024Quote: ... for genotype analysis was extracted using the Qiagen DNeasy Blood and Tissue kit and PCR analysis was done with CloneAmp™ HiFi PCR Premix (Takara) or Phusion polymerase (NEB).
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bioRxiv - Microbiology 2024Quote: ... Transfection was performed using 4 µL TransIT 293 (Takara, Shiga, Japan) and 100 µL OPTI-MEM (Thermo Fisher Scientific) ...