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Citations for Takara Bio :
2201 - 2250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... Target sequences were amplified in a first PCR using gene-specific primers (F1 and R1) and EmeraldAmp GT PCR Master Mix (TaKaRa RR320A). The PCR conditions were ...
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bioRxiv - Zoology 2024Quote: ... Sequencing library fragments in the range of 400–1,000 bp were cut and purified with a NucleoSpin Gel and PCR Clean-up kit (Clontech) and sequenced using a NextSeq500 sequencer with HighOutputMode 75 cycles kit (Illumina) ...
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bioRxiv - Pathology 2024Quote: Expression for IFN-related genes was conducted by reverse transcription using the PrimeScript RT Reagent Kit (Perfect RealTime, Takara Bio Inc.). qPCR reaction was performed in duplicate using Takyon ROX SYBR MasterMix blue dTTP (Eurogentec ...
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bioRxiv - Plant Biology 2024Quote: The full-length FLP1 cDNA in pENTR/D-TOPO was cloned into pDEST_GBKT7 vector85 by Gateway reaction and transformed into Y2HGold yeast strain (Takara Bio). The resultant yeast was cultured in synthetic defined medium (–W) ...
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bioRxiv - Immunology 2024Quote: ... SMART-Seq HT Kit (Takara) was used to construct cDNA and libraries ...
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bioRxiv - Plant Biology 2024Quote: RT‒PCR splicing analysis was carried out with Ex-Taq polymerase (Takara, Japan) for a total of 25-28 cycles ...
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bioRxiv - Neuroscience 2024Quote: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); guinea pig anti-DsRed (1:500 ...
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bioRxiv - Genetics 2024Quote: ... Genomic regions were subsequently amplified using EpiTaq HS (Takara Bio, Shiga, Japan) and cloned into the pGEM-T Easy vector (Promega ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from tissues using RNAiso Plus (Takara). The extracted RNA was quantified by Nanodrop and 500 ng of it was converted into cDNA using a PrimeScript™ RT reagent Kit (Takara) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA fragments of approximately 1.5 kb upstream and downstream of LBDG_21500 required for homologous recombination were amplified via polymerase chain reaction (PCR; PrimeSTAR Max DNA polymerase, Takara, Shiga, Japan) using L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were constructed via site-directed mutagenesis using the PrimeSTAR Mutagenesis Basal Kit (Takara) with p21500_complement as the template ...
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bioRxiv - Neuroscience 2024Quote: The samples were rRNA depleted and prepared for sequencing using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio, France). In brief ...
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bioRxiv - Bioengineering 2024Quote: ... Diluted plasmid vector DNA was added to Lenti-X packaging single shots (TaKaRa; Cat.no: 631276) and vortexed for 20 sec at high speed ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized with isoflurane and perfused transcardially with 1X Phosphate Buffered Saline (PBS) (Takara) and 4% Paraformaldehyde (PFA ...
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bioRxiv - Genetics 2024Quote: Transcription Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Quantitative RT-PCR (qRT-PCR) was performed using SYBR Green PCR Master Mix (Takara) in an Applied Biosystems QuantStudio 3 ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using the ThruPLEX DNA-Seq Kit and DNA Unique Dual Index Kit (Takara) according to the manufacturer’s manual ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and upstream fragment) were connected using the In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, Shiga, Japan) to construct the LBDG_21500-disrupting plasmid (Table S9).
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bioRxiv - Neuroscience 2024Quote: ... The brains were then incubated with primary antibodies (anti-GFP chicken Abcam 1:1000 or 1:2000, Cat#13970; anti-dsRed rabbit Takara 1:250 ...
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bioRxiv - Cancer Biology 2024Quote: ... Lenti-X Concentrator (Takara Bio PT4421-2) was added at a 1:3 volume ratio with clarified supernatant (1 part Lenti-X Concentration ...
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bioRxiv - Cancer Biology 2024Quote: Cancer cells were transduced with RetroNectin reagent (Takara Bio T100B) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Fragment (∼50 ng) and linearised vector (∼50 ng) were combined with 2 µL 5X In-Fusion HD Enzyme (Takara Bio, #938910) and mixture made up to 10 µL with Nuclease Free Water (Fisher Bioreagents ...
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bioRxiv - Immunology 2024Quote: ... Library preparation of RNA samples was performed using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara 634485). Two independent pooled libraries (samples from mice with spontaneous disease and samples from mice with pristane-induced disease ...
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bioRxiv - Immunology 2024Quote: Ripk3-2XFVfl/fl Aldh1l1 Cre+ mice and Cre− littermate controls were treated intraperitoneally with B/B Homodimerizer (Takara, #AP20187) for 24 hours prior to perfusion with PBS and harvesting of whole brain ...
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bioRxiv - Immunology 2024Quote: ... Lenti-X 293T cells (Clontech) were co-transfected with pMDL (Addgene #12251) ...
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bioRxiv - Immunology 2024Quote: ... the sequence encoding the N-terminal Avi-tag was removed from the expression plasmid using In-Fusion® cloning (Takara #638948) prior to protein expression and purification ...
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bioRxiv - Molecular Biology 2024Quote: Strand-specific RNA-seq was performed on parental and ibrutinib-resistant Rec-1 cells using SMARTer Stranded Total RNA Sample Prep Kit (Takara, cat# 634873) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were created using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio Inc.). DNA samples were repaired ...
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bioRxiv - Molecular Biology 2024Quote: ... The incubated DNA samples were purified with the NucleoSpin Gel and PCR Clean-up kit (#740609.250; Takara Bio Inc.). The purified DNA samples were used to prepare the library ...
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bioRxiv - Molecular Biology 2024Quote: ... and the insert fragments fused to the linearized plasmids using the In-Fusion Cloning Kit (Takara Bio Inc., Shiga, Japan), according to the protocol provided by the manufacturer with the Escherichia coli JM109 strain ...
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bioRxiv - Microbiology 2024Quote: ... The amplified linear fragment of pKNOCK-Km and the internal fragments of each gene were fused using In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and SacI (Takara Bio), and circularized DNA prepared by self-ligation with Ligation high Ver.2 (Toyobo ...
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bioRxiv - Microbiology 2024Quote: ... pRF vectors with chimeric L-segments were constructed by in-fusion high-fidelity (HD) restriction-free cloning method (TaKaRa Bio). Two nucleotides of LF2384 L gene were different from those of parent wildtype virus and one nucleotide of LF2350 GPC gene of was different from that of wildtype one ...
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bioRxiv - Microbiology 2024Quote: ... or the empty vector as control (Takara, 632105). 48 h after transfection ...
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bioRxiv - Microbiology 2024Quote: ... then introduced between XhoI and AgeI sites of pCMV-FLAG vector (Takara, 632105). Resulting plasmids containing the desired mutation were confirmed by DNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... 2 U/µl of Ribonuclease Inhibitor (Takara Inc), 20 ng/µl of plasmid DNA ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was performed with gene-targeted primers using TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara #RR82WR) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... These fragments were then cloned into the CMV/R plasmid containing the VRC01 HC and LC constant domains by In-Fusion (Takara Bio). DNA encoding the wild-type SARS-CoV-2 Wuhan Hu-1 receptor-binding domain (residues 319-533 ...
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bioRxiv - Microbiology 2024Quote: ... the 2nd PCR product was amplified from 10 ng purified 1st PCR product using the TaKaRa Ex Taq HS (TaKaRa Bio Inc., Japan; #RR006A) with a forward (5’-AATGATACGGCGACCACCGAGATCTACAC-Index2-ACACTCTTTCCCTACACGACGC ...
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bioRxiv - Cancer Biology 2024Quote: ... Lenti-X Concentrator (Takara, 631232) was used to concentrate lentivirus ...
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bioRxiv - Cancer Biology 2024Quote: ... after which the lentivirus concentration was measured by p24 ELISA (Takara, 632200). Following overnight transduction ...
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bioRxiv - Cell Biology 2024Quote: ... and then the cleared supernatant was concentrated with Lenti-X Concentrator (Cat# 631232, Takara) and resuspended in 1 mL of DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were constructed using a SMARTer Stranded Total RNA-Seq kit (Clontech). Directional mRNA-Seq of sorted cell populations was conducted using the Illumina HiSeq 6000 system ...
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bioRxiv - Cell Biology 2024Quote: A total of 250 pg to 10 ng RNA per sample was used as input material for sequencing libraries (1–500 ng for small RNA libraries) using the SMARTer Stranded Total RNA-Seq Kit V2 (Takara Bio USA, Inc.) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were stripped using Western BLoT Stripping Buffer (Takara Bio, #T7135A) and re-probed with the desired antibody ...
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bioRxiv - Immunology 2024Quote: ... plasmids were transformed into StellarTM cells (Takara Bio) and isolated using Maxiprep kits (NucleoBon Xtra Maxi kit ...
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bioRxiv - Microbiology 2024Quote: ... Clones were screened for successful insert ligation by colony PCR using EmeraldAmp GT PCR master mix (Takara Bio) with T7 promoter and T7 terminator primers ...
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bioRxiv - Microbiology 2024Quote: ... and 2.5 mg/mL Yatalase (Takara, Shiga, Japan) at 30 °C with shaking at 83 rpm for 3 h ...
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bioRxiv - Cancer Biology 2024Quote: ... using primers listed in Table S1 (designed using the Takara in-Fusion Cloning Design Primer tool (https://takara.teselagen.com/#/DesignPage)) ...
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bioRxiv - Cancer Biology 2024Quote: ... CASP3 open reading frame and BioID2 either in N-terminus or in C-terminus of CASP3 were inserted into a pcDNA3 vector using the In-Fusion® HD Cloning kit (Takara, 639649) according to the manufacturer’s instructions ...