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Citations for Takara Bio :
2151 - 2200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent Escherichia coli (Takara Bio, 636766). Plasmids were purified by miniprep (QIAGEN ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were then isolated through gel electrophoresis and purified using the Nucleospin gel purification kit (Takara Bio,740609.250). Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent Escherichia coli (Takara Bio ...
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bioRxiv - Biophysics 2024Quote: ... and the supernatant was incubated with TALON IMAC resin (Clontech) and 20 mM imidazole for 12 h at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... Virus was concentrated using Retro-X Concentrator (Takara, 631456). The following day ...
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bioRxiv - Bioengineering 2024Quote: ... Bead-stimulated cells were transferred to RetroNectin-coated (Takara) virus–containing plates and incubated overnight ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... tissue was incubated overnight at room temperature in rabbit anti-DSred (Clontech; 1:2500) and mouse anti-TH (Immunostar ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were expressed in suspension Expi293F cells at 37 °C and purified under native conditions with TALON metal affinity resin beads (Takara) and SEC experiments ...
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bioRxiv - Biochemistry 2024Quote: N-terminally HA-tagged IPMK (WT or the kinase deficient D144A mutant63) were cloned into pLPCX (Clontech). VSVg pseudotyped MLV was prepared by transfecting pLPCX-HA-IPMK along with pMLV-Gag-Pol and pVSVg into HEK293T cells using PolyJet transfection reagent (SignaGen) ...
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bioRxiv - Biochemistry 2024Quote: ... The primers were as follows: Cars2 (forward, MA184847-F; reverse, MA184847-R; Takara Bio); Gapdh (forward ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was then synthesized with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: We used TB Green Premix Ex Taq [(Takara Bio) and a StepOnePlus Real-Time PCR System (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... Gapdh (forward, MA050371-F; reverse, MA050371-R; Takara Bio); and Actb (forward ...
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bioRxiv - Biochemistry 2024Quote: ... for A3>P: 5’-aaaacacugaaccug-3’ for A2>P: 5’-aaacacugaaccug-3’) were incubated with 10 U recombinant MazF (Takara Bio) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The hZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech) in which the downstream EGFP gene was deleted and an HA tag was added at the C-terminus ...
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bioRxiv - Biochemistry 2024Quote: RNA was purified from transiently transfected HEK293-FT cells by NucleoSpin RNA (Takara) and the concentrations determined by Nanodrop One (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... wild-type (WT) MDCK cells were transfected with pAcGFP1-Mem vector (Takara, 632509). To decouple the LINC complex by overexpressing dominant-negative KASH protein ...
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bioRxiv - Biophysics 2024Quote: ... The amplified products were digested with BamHI and EcoRI (TaKaRa Bio, Shiga, Japan). The fused FP genes ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated with TALON® Metal Affinity Resin (Clontech, #635502) at 4°C for 30 minutes and the resin was washed with six column volumes of wash buffer A (50 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was synthesized using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara Bio) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... PCRs were performed using the PrimeSTAR Max DNA Polymerase (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP libraries were prepared with the ThruPLEX® DNA-Seq Kit (Takara) and sequenced on the Illumina NovaSeq6000 SP-100 platform ...
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bioRxiv - Cancer Biology 2024Quote: KTC1 and PE121410 cells with RBM10 loss-of- function mutations were used to generate dox-inducible RBM10 expressing cells (KTC-RBM10 and PE-RBM10) by transducing the pLVX-Tet-On Advanced vector system (Clontech) with RBM10 cDNA (NM_005676 ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant protein was then purified by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and ...
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bioRxiv - Biochemistry 2024Quote: Construct preparation – Mammalian expression plasmids encoding fluorescent nanobody constructs were created using InFusion cloning (Takara Bio). DNA fragments encoding the open reading frame (ORF ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... The proteins were purified on a TALON Cell-Thru His-tag affinity column (Clontech) in a buffer containing 10 mM Na Phosphate (pH 7.0) ...
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bioRxiv - Biochemistry 2024Quote: ... pUB83 is a lentiviral expression vector based on the pLVX-PURO (Clontech) plasmid containing a CMV promoter ...
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bioRxiv - Bioengineering 2024Quote: ... Bacterial and mammalian expression plasmids for CODEs were cloned using In-Fusion® cloning (Takara Bio, Cat# 638948). Q5 high-fidelity polymerase (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... PrimeSTAR® GXL DNA Polymerase (Takara Bio, Cat# R050A) was used for amplification ...
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bioRxiv - Biochemistry 2024Quote: ... and cDNA was synthesized using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara Bio) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... Lenti-X 293T cells were purchased from Takara and cultured according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Primers with overhangs (Table S2) compatible with In-Fusion HD cloning kit (Takara Bioscience) were used to clone full-length genes into pESC-HIS (GenBank ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a 5 ml column of TALON® Metal Affinity Resin (TAKARA) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... We introduced a T44C mutation and 108C mutation into the GB1-loop by site-directed mutagenesis using a PrimeSTAR mutagenesis basal kit (Takara Bio).
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bioRxiv - Neuroscience 2024Quote: The rep/cap plasmids pRC1 (TaKaRa Bio), pAAV2/5 (gifted from Melina Fan [Addgene plasmid # 104964 ...
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bioRxiv - Neuroscience 2024Quote: ... pRC2-mi342 used to produce the AAV2/2 vector was a plasmid included in the AAVpro Helper Free System (Takara Bio). The AAV2/9 plasmid was kindly provided by James M ...
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bioRxiv - Neuroscience 2024Quote: ... The PCR-amplified promoter fragments were inserted into the XhoI-AgeI site of the pAAV using Ligation high Ver.2 (LGK-201; Toyobo: hGFAP(ABC1D) or In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan: mMBP promoter).
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bioRxiv - Neuroscience 2024Quote: ... PCR amplification of the fragments and their subsequent ligation was performed using the In-Fusion cloning kit and online tools (BD Clontech, Takara Bio, USA), or they were synthesized by GenScript (Piscataway ...
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bioRxiv - Plant Biology 2024Quote: ... an anti-GFP monoclonal antibody (Takara, 632592) was used ...
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bioRxiv - Biochemistry 2024Quote: ... Saccharomyces cerevisiae strain EGY48 (Matchmaker 3 system, Clontech) was used to perform yeast two-hybrid library screen ...
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bioRxiv - Biochemistry 2024Quote: ... Plant targets identified in the yeast-two hybrid screen were cloned as translational fusions with the activation domain in pB42AD (Clontech) for verification ...
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bioRxiv - Biochemistry 2024Quote: Plasmids from interaction candidates were extracted according the manual (Clontech). The selected prey candidates were sequenced and identified by comparison to the A ...
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bioRxiv - Biochemistry 2024Quote: ... cerevisiae strain EGY48 was transformed with Tue1-LexA-pGILDA (Clontech, MATCHMAKER LexA Two-Hybrid System) and tested for auto-activity ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane was then incubated with 1:1000 mouse anti-His6 antibody (Clontech) for 15 mins ...
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bioRxiv - Biochemistry 2024Quote: ... Induction of the constructs was done by supplementing the medium with 0.5 µg ml-1 tetracycline (Tet) (Takara Bio).
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bioRxiv - Neuroscience 2024Quote: ... The presence of viral particles was confirmed with Lenti-X GoStix Plus test (Takara).
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bioRxiv - Bioengineering 2024Quote: ... Lenti-XTM 293T cells (TaKaRa; Cat.no 632180) were transfected with pLVX-IRES-Hygro-CD81-Snorkel-tag plasmid using Lenti-X packaging single shots according to manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Transfer clarified supernatant to fresh container and combine 1 volume of Lenti-X concentrator (TaKaRa; Cat.no: 631232) to 3 volumes of clarified supernatant ...
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bioRxiv - Microbiology 2024Quote: ... coli DH-5α competent cells (Takara). Ultimately ...
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bioRxiv - Microbiology 2024Quote: ... 90 μl of RNase-free water was added and then 2.5 μl of diluted sample was used for real-time RT-PCR according to the manufacturer’s protocol with One step TB green PrimeScript PLUS RT-PCR Kit (Takara) and primers for the nucleocapsid (N ...
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bioRxiv - Microbiology 2024Quote: ... SISPA PCR amplification was conducted with TaKaRa Taq DNA Polymerase (Takara) using the primer FR20RV (5’-GCCGGAGCTCTGCAGATATC-3’ ...