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Citations for Takara Bio :
2101 - 2150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Four micrograms of total RNA were treated with recombinant DNaseI (TaKaRa) to eliminate genomic DNA ...
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bioRxiv - Plant Biology 2024Quote: ... The Prime Script TM RT reagent Kit (TAKARA) was used to synthesize the first-strand cDNA ...
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bioRxiv - Plant Biology 2024Quote: ... which was performed using a SMARTer RACE 5’/3’ Kit (Clontech Laboratories, Mountain View, CA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Complementary DNA (cDNA) was generated from total RNA using PrimeScript RT Master Mix (Takara Bio Inc., Japan). RT-qPCR was performed on a Stratagene Mx3005P qPCR machine using a Takyon™ No ROX SYBR 2X MasterMix Blue dTTP (Eurogentec ...
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bioRxiv - Cancer Biology 2024Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) following the instructions of PrimeScript RT reagent kit (Takara, Beijing, China). qRT-PCR was performed using the AceQ Universal SYBR qPCR Master Mix (Vazyme) ...
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bioRxiv - Bioengineering 2024Quote: ... The annealed product was added with 35 U of T4 DNA Ligase™ (Takara Bio Inc.), and incubated at 37°C for 1 h and then at 16°C for O/N ...
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bioRxiv - Bioengineering 2024Quote: ... Libraries were prepared using SMART-Seq v4 Ultra Low Input (Takara) and Nextera XT DNA Library Preparation (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... Transformation was performed with purified PCR products in Stellar Competent Cells (Takara, 636763). Primers used for PCR and sequencing are listed below:
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bioRxiv - Biochemistry 2024Quote: ... and dNTP Mix (Takara, SD7178). 50 µL of PCR product was digested by 1μL of DpnI (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast transformation and growth assay were performed as described in the Yeast Protocols Handbook (BD Clontech, USA). In brief ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast transformation and liquid assay were conducted as described in the Yeast Protocols Handbook (BD Clontech, USA). The primers used for the Y1H assay are shown in Table S4.
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bioRxiv - Genomics 2024Quote: ... in SeqAmp CB PCR buffer (Takara Bio., 638526) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... we generated a second strand by performing one PCR cycle using only the reverse primer with Seqamp DNA polymerase (Takara Bio., 638504) in SeqAmp CB PCR buffer (Takara Bio. ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using Sapphire Amp PCR Mastermix (Takara Bio, RR350B). For SftpcCreERT2 ...
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bioRxiv - Bioengineering 2024Quote: ... The purified PCR product was cloned with a C-terminus 6-His tag into pET22b vector via NdeI/XhoI using the In-Fusion HD Cloning kit following the manufacturer’s protocol (Takara Bio). The construct was transformed into chemically competent E ...
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bioRxiv - Biochemistry 2024Quote: ... This PCR was performed by PrimeSTAR Max polymerase (Takara Bio) with the following settings ...
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bioRxiv - Immunology 2024Quote: ... Lentiviral supernatants were concentrated using Lenti-XTM Concentrator (Takara Bio, Cat # 631232) and then centrifuged at 1500g for 30 minutes at 4°C and supernatant was discarded ...
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bioRxiv - Neuroscience 2024Quote: ... was transfected into Lenti-X 293T cells (TaKaRa, #632180) using the polyethylenimine(PEI)-based Transporter 5 transfection reagent (Polysciences ...
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bioRxiv - Biochemistry 2024Quote: The various proteins were produced in Escherichia coli strain BL21(DE3)-pGro7/GroEL strain (TaKaRa). The sequences were enhanced by an N-terminal strep tag (WSHPQFEK ...
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bioRxiv - Genomics 2024Quote: ... Lentivirus was concentrated using Lenti-X Concentrator (Takara Bio cat no. 631232), resuspended in StemSpan SFEM II supplemented with 1% BSA and stored at –80 °C until use ...
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bioRxiv - Genomics 2024Quote: ... filtered retroviral supernatants were harvested and applied to HSCs (extracted as described above) in plates coated with Retronectin (20 μg/ml; Takara Bio; #T100B). The transduction process involved spinning at 2,000g for 2 hours at 32°C ...
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bioRxiv - Biochemistry 2024Quote: ... PrimeSTAR GXL Buffer (Takara, SD1967), and dNTP Mix (Takara ...
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bioRxiv - Microbiology 2024Quote: ... accessory proteins coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (Clontech) to generate pseudotyped lentiviral particles 66,67 ...
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bioRxiv - Cancer Biology 2024Quote: TET-ON-Cas9-inducible expressing cells were generated firstly by infection with the rtTA plasmid pLVX-Tet3G (Takara, 631358) and neomycin selection ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by extraction with Trizol Reagent (Takara, Dalian, China) to obtain RNA.
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bioRxiv - Bioengineering 2024Quote: ... We confirmed the cells were mycoplasma-negative using a Cycleave PCR Mycoplasma Detection Kit (Takara Bio, CY232).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Site-directed mutagenesis was performed in the seed sequences of Mecp2 using PrimeStar Max DNA Polymerase (R045, Takara, Japan) for PCR amplification ...
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bioRxiv - Biochemistry 2024Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio). The sequences of all primers in this study can be found in Table S3 ...
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bioRxiv - Biochemistry 2024Quote: ... this was cloned into pALiCE02 using In-fusion cloning (Takara). Amplification of FGFR3-TACC3.RT112.pALiCE02 involved transformation into One Shot™ OmniMAX™ 2 T1 R competent cells (Thermo ...
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bioRxiv - Bioengineering 2024Quote: Restriction endonucleases were purchased from Takara (China) and NEB (USA) ...
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bioRxiv - Biochemistry 2024Quote: ... EB3 was purified through His60 Ni Superflow resin (Takara Bio Inc.) and eluted from the column by cleavage of the His-tag at the PreScission site ...
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bioRxiv - Molecular Biology 2024Quote: ... Final NGS libraries were generated using 2ng of the purified 1st PCR product using the dual-indexing Illumina-compatible DNA HT Dual Index kit (Takara #R4000660,R400661). 2nd PCR products were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... LTR amplicons or gBlocks™ Gene Fragments were mixed with the pLd2G backbone (gLTR_OUT) and genomic (gLTR_IN) amplicons and joined in a single reaction using In-Fusion® HD Cloning Kit (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... The DDX54 wobble mutant was generated by site-directed mutagenesis using PrimeSTAR Max and In-Fusion cloning (Takara). All primers are available in the Supplemental Information ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were treated with premix WST-1 (Takara, Catalog number: MK400) to determine cell viability following manufacturer’s instructions and incubated at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: The genomic titers of the viral vectors were determined by real-time quantitative PCR using Thermal Cycler Dice Real Time System II TP900 or III TP970 (Takara Bio Inc.) and Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... PCR amplification of the fragments and their subsequent ligation was performed using the In-Fusion cloning kit and online tools (BD Clontech, Takara Bio, USA), or they were synthesized by GenScript (Piscataway ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted using TRIzol (Takara), and 500 ng of RNA was reversed to cDNA with PrimeScriptTMRT Master Mix (Takara) ...
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bioRxiv - Immunology 2024Quote: ... RNA was converted to cDNA using PrimeScriptRT reagent Kit with gDNA Eraser (Takara, #RR047A), and used as a template for qPCR with TB Green® Premix Ex TaqTM II (Tli TNaseJ Plus ...
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bioRxiv - Plant Biology 2024Quote: ... about 1µg to 5µg total RNA was used for first-strand synthesis using RNA to cDNA EcoDry Premix with Oligo(dT) (Takara Bio Inc., Japan), by following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... CRISPR-targeted regions in marcks.L/S and marcksl1.L/S were PCR-amplified using the EmeraldAmp GT PCR Master Mix (Takara Bio). For every gene ...
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bioRxiv - Cell Biology 2024Quote: ... and the plasmids encoding GFP (Clontech, 6085-1), the pOPINE GFP nanobody (Addgene ...
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bioRxiv - Plant Biology 2024Quote: ... inserted into the BamHI/BglII site of pGAD424 (Clontech) to generate corresponding AD-HSF fusion vectors and identified by sequencing.
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bioRxiv - Plant Biology 2024Quote: The target DNA fragments were obtained by PCR with the Taq enzyme (Takara, Japan) and subsequently cloned and inserted into the corresponding vectors with the ClonExpress II One Step Cloning Kit (Vazyme Biotech ...
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bioRxiv - Plant Biology 2024Quote: ... and mHSE were synthesized by Shanghai Sangon Biotechnology and subsequently cloned and inserted into pHIS2.1 (Clontech). The PCR products were cloned and inserted into the EcoRI/SpeI site of the pHIS2.1 vector (Clontech ...
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bioRxiv - Plant Biology 2024Quote: ... The coding region of S-HsfA4c lacking the stop codon TAG was amplified from cDNA using the primers S-HsfA4c-GFP-F/-R and cloned and inserted into the BglII/SpeI sites of pCAMBIA1302 (Clontech) to generate 35S:S-HsfA4c-GFP ...
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bioRxiv - Plant Biology 2024Quote: ... The coding region of S-HsfA2 lacking the stop codon TAG was amplified from genomic DNA using the primers S-HsfA2-GFP-F/-R and cloned and inserted into the NcoI/SpeI sites of pCAMBIA1302 (Clontech) to generate 35S:S-HsfA2-GFP.
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bioRxiv - Plant Biology 2024Quote: ... The corresponding PCR products were subsequently cloned and inserted into the EcoRI/PstI sites of pGBKT7 (Clontech) to generate the BD-S-HsfA2 ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR products were cloned and inserted into the EcoRI/SpeI site of the pHIS2.1 vector (Clontech) and sequenced using pHIS2.1 forward or reverse primers.
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bioRxiv - Bioengineering 2024Quote: ... Linear DNA fragments were produced by PCR using HiFi polymerase (Takara, 639298), followed by treatment with DpnI to remove template DNA ...