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Citations for Takara Bio :
2001 - 2050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... This fragment was cloned into the pLVX-EF1α-IRES-Puro vector (Clontech, #631988) by first treating with the EcoR1-HF (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was reverse transcribed to cDNA with M-MLV reverse transcriptase kit (Takara). LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR in the presence of SYBR Green (TaKaRa TB Green Primer Ex TaqII, #RR820Q) was carried out according to the manufacturer’s instructions in a BioRAD CFX 384 Thermal Cycler (BioRAD ...
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bioRxiv - Plant Biology 2024Quote: ... The indicated genes were PCR amplified according to the manufacturer’s conditions using Titanium Taq DNA polymerase (Takara Bio) using the following primers ...
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bioRxiv - Microbiology 2024Quote: ... PrimeScriptTMRT Master Mix and TB Green® Premix Ex Taq™ were purchased from Takara (Japan); Triton-X100 and L-citrulline were purchased from Solarbio (China) ...
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bioRxiv - Microbiology 2024Quote: ... and 500 ng of RNA was reversed to cDNA with PrimeScriptTMRT Master Mix (Takara). RT-qPCR was performed with 100 ng of the above cDNA as a template ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the Takara NucleoBond RNA Soil kit (Takara Bio USA, CA) with 5g of soil as input ...
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bioRxiv - Neuroscience 2024Quote: ... were coated with 15 µg of EGFP-N1 (Clontech), or 10 µg of tDimer-dsRed together with 16 µg SEP-GluA224 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (1:2000, 632496, Clontech); guinea pig anti-doublecortin (Dcx ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic titer was determined by Lenti-X GoStix Plus (Takara). Lentivirus was functionally titrated previously using parallel preparation of GFP-expressing viral particles (Campeau et al. ...
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bioRxiv - Neuroscience 2024Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), trans-plasmid providing viral capsid PHP.(e)B (a generous gift from Viviana Gradinaru (Addgene plasmid #103005 ...
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bioRxiv - Neuroscience 2024Quote: ... Input libraries were prepared using ThruPLEX (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Viral titer (p24 core antigen content) was determined initially using a LentiX GoStix Plus titration kit (Takara #631280) and subsequently by HIV p24 AlphaLISA detection kit (PerkinElmer #AL291C) ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was transfected into nearly confluent (80-90%) 10 cm2 plates of low-passage LentiX 293T (Takara #632180) using a calcium phosphate transfection protocol ...
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bioRxiv - Neuroscience 2024Quote: ... genes of interest were amplified using a PrimeSTAR GXL kit (Clontech, CA, USA). The amount of cDNA was normalized according to β-actin2 amplification in qRT-PCR and RT-PCR experiments ...
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bioRxiv - Neuroscience 2024Quote: ... B/B Homodimerizer (200nM, Takara, 635058), L-Glutamic acid (100-1,000μM ...
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bioRxiv - Neuroscience 2024Quote: ... Amplicons were inserted into a pAAV-CMV vector using In-Fusion cloning (Takara Bio). Plasmids were verified with Sanger sequencing (Genewiz ...
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bioRxiv - Neuroscience 2024Quote: The genomic region (813 bp) containing the Tbr2-crRNA target sequences was PCR amplified using PrimeSTAR Max (Takara Bio, Shiga, Japan), and a pair of primers ...
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bioRxiv - Neuroscience 2024Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), trans-plasmid providing viral capsid PHP.(e)B (a generous gift from Viviana Gradinaru (Addgene plasmid #103005 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA cloning libraries were created using SMARTScribe™ Reverse Transcriptase (Takara/Clontech Cat # 639537) and the Advantage 2 PCR kit (Takara/Clontech Cat # 639207) ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of produced viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Neuroscience 2024Quote: ... PCR products were amplified from neuronal tissue cDNA libraries (see above) using TaKaRa LA Taq® DNA Polymerase (Takara Cat # RR002A) PCR products were purified using the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... and the Advantage 2 PCR kit (Takara/Clontech Cat # 639207). Libraries were checked for quality and approximate concentration on a 1% agarose gel.
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bioRxiv - Neuroscience 2024Quote: ... and the Advantage 2 PCR kit (Takara/Clontech Cat # 639207). Libraries were checked for quality and approximate concentration on a 1% agarose gel.
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bioRxiv - Neuroscience 2024Quote: ... and a total of 0.9 μg of cDNA vectors encoding the GluN1/GluN2 receptors and GFP (to identify the transfected cells; pQBI 25, Takara, Shiga, Japan). After transfection ...
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bioRxiv - Immunology 2024Quote: ... 0.5 µl of 40 U/ µl RNase Inhibitor and 0.5 µl of the SMARTScribe RT (Takara, 639537), added the mix to the RNA mix and incubated the reaction at 42 °C for 60 min ...
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bioRxiv - Plant Biology 2024Quote: ... Each transcript extracted was then cloned into a pRACE cloning vector (Clontech Laboratories) with an In-Fusion Cloning Kit (Clontech Laboratories) ...
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bioRxiv - Developmental Biology 2024Quote: ... SMART-Seq v4 Ultra low input RNA Kit (Takara, #634889) was used for first-strand and second-strand cDNA synthesis and double-stranded cDNA end repair ...
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bioRxiv - Cell Biology 2024Quote: Lenti-X HEK-293Ts (Takara) were used for lentivirus production and maintained at below 80% confluency in DMEM supplemented with 10% (v/v ...
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bioRxiv - Plant Biology 2024Quote: ... This suspension was used to determine total protein concentrations using Takara BCA Protein Assay Kits (TaKaRa, Kusatsu, Japan).
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bioRxiv - Plant Biology 2024Quote: ... Proteins blotted on membranes were visualized using TaKaRa CBB Protein Safe Stain (TaKaRa) and chemiluminescence and CBB staining were visualized using ImageQuant LAS 500 mini (Cytiva).
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bioRxiv - Plant Biology 2024Quote: ... The qRT-PCR was performed with SYBR Premix Ex TaqTM (TaKaRa) on a LightCycler 480 II (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... Extracted RNA was reverse transcribed to cDNA using a PrimeScriptTM RT reagent Kit (TaKaRa). The qRT-PCR was performed with SYBR Premix Ex TaqTM (TaKaRa ...
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bioRxiv - Biochemistry 2024Quote: ... The In-Fusion Snap Assembly (Takara Bio) was used as the standard method for cloning according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... coli strain BL21 (DE3) (NIPPON GENE) co-transformed with a pGro7 chaperone plasmid (Takara Bio) and purified as follows ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation experiments utilized pEGFP-N1 (Clontech, 6085-1), DCC-pHluorin (Plooster et al. ...
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bioRxiv - Neuroscience 2024Quote: ... Purification Kit (Takara) was used for viral particle purification ...
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bioRxiv - Neuroscience 2024Quote: ... PCR was performed using Phusion (Takara), following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... were purchased from Thermo Fisher Scientific. cDNA synthesis kit (cat no. 6110B) and real-time PCR kit (cat no. PN 4367659) were purchased from TAKARA. ECL reagent (cat no ...
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bioRxiv - Neuroscience 2024Quote: ... The open reading frame of HTH was constructed in combination with the pGADT7 vector (Clontech), which served as the effector construct ...
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bioRxiv - Neuroscience 2024Quote: ... along with 0.4 μg of pmCherry-N1 (632523, Takara-bio, Shiga, Japan) and 0.4 μg of bcl-xl/pcDNA3 (Konishi et al. ...
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bioRxiv - Neuroscience 2024Quote: ... 2 μg of pEGFP-C1 (Takara-bio, discontinued by supplier), and 1.5 μg of bcl-xl/pcDNA3 in 70 μl of DMEM ...
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bioRxiv - Plant Biology 2024Quote: ... Transformed yeast cells were grown under selective conditions in a minimal medium (46.7 g L-1 Minimal SD Agar Base [TaKaRa], 0.78 g L-1 uracil dropout supplement [TaKaRa]). Cytokinin transport assays were performed according to the method described by Zhao et al ...
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bioRxiv - Plant Biology 2024Quote: ... Transformed yeast cells were grown under selective conditions in a minimal medium (46.7 g L-1 Minimal SD Agar Base [TaKaRa] ...
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bioRxiv - Plant Biology 2024Quote: ... Total protein concentration was determined with a Bradford Protein Assay Kit (Takara Bio Inc.) according to the microtiter plate protocol recommended by the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: Y2H assays were conducted following the protocols outlined in the Matchmaker GAL4 Two Hybrid System (Clontech). Clone the full-length CDS of each target gene into pGADT7 and pGBKT7 vectors ...
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bioRxiv - Physiology 2024Quote: ... S1A) using the In-Fusion HD system (Clontech, #639650) (19) ...
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bioRxiv - Neuroscience 2024Quote: ... D9891)-inducible adenoviral constructs were generated for Halo-TRIM9 and UNC5C-pHmScarlet using the adeno-X™ system 3 (Takara Bio, 631180), using the detailed protocol outlined by (O’Shaughnessy et al. ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then transferred to primary antibody solution containing rabbit anti-dsRed (1:1000, Takara, Catalog #: 632496, RRID:AB_10013483) in 0.3% Triton PBS with 3% normal goat serum for 2 nights at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... and amplified by PCR using Takara Premix Ex TaqTM Hot Start Version (Takara Research, RR030A, Japan). The amplified products were recovered from an agarose gel using a Universal DNA Purification Kit (TIANGEN ...