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Citations for Takara Bio :
2051 - 2100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... This was cloned as an AscI/PacI fragment into the multiple cloning site downstream of the EF1a promoter in the pLVX-EF1α-IRES-ZsGreen1 Vector (Takara). The WT allele was generated by site-directed mutagenesis using the following 2 primers (Forward 5’-atttgtggactcctacgatcCaaccatagaaaacactttta-3’ and Reverse 5’-taaaagtgttttctatggttGgatcgtaggagtccacaaat-3’.
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bioRxiv - Neuroscience 2024Quote: ... NPCs and STRpcs were treated with either AP21967 (1 nM) (Takara, 635055) or Rapamycin (1 nM ...
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bioRxiv - Neuroscience 2024Quote: ... AAV6 was extracted and purified from 293T using AAVpro purification kit (Takara, 6666) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-RFP antibodies (1/1000, RT, overnight, rabbit anti-DsRed, 632496, Takara Bio USA, Madison, WI), followed by Alexa-488 (1/100 ...
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bioRxiv - Plant Biology 2024Quote: ... All qRT-PCRs was performed using SYBR Green I (Takara SYBR Premix Ex Taq) on the Bio-Rad CFX96 Real-time PCR detection system.
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bioRxiv - Molecular Biology 2024Quote: ... and pVSV-G (Clontech) constructs in 6:3:1 weight ratios by X-Treme Gene HP Transfection Reagent (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... and In-Fusion HD Cloning kit (Clontech), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... via XhoI and Kpn1 double digest and inserted into a pEGFP-N3 (Clontech) backbone and validated by sanger sequencing ...
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bioRxiv - Biophysics 2024Quote: ... Talon resin (Clontech) was added to the solubilized membranes and incubated for at least 2 h or overnight under gentle agitation.
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bioRxiv - Neuroscience 2024Quote: ... Ultra-low input RNA sequencing library was prepared by using SMART-Seq HT kit for full-length cDNA synthesis and amplification (Takara, San Jose, CA, USA), and Illumina Nextera XT (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... single cells were sorted into 8-well strips containing SMART-Seq lysis buffer with RNase inhibitor (0.17 U/µL; Takara Cat#ST0764) and were immediately frozen on dry ice for storage at −80°C ...
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bioRxiv - Neuroscience 2024Quote: Sample preparation for SMART-Seq was performed using the SMART-Seq v4 kit (Takara Cat#634894) as described previously10 ...
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bioRxiv - Neuroscience 2024Quote: ... 1µg of total RNA was mixed with a total volume of 10µl of cDNA synthesis kit (TaKaRa 6110A, Tokyo) consisting of dNTP mixture ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies included rabbit anti-DsRed (Takara Bio, catalog #632496, 1:1000) and chicken anti-NeuN/FOX3 (EnCor ...
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bioRxiv - Neuroscience 2024Quote: ... Viral supernatants were concentrated using Lenti-X Concentrator (Takara) and spun for 45 minutes at 1500 x g ...
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bioRxiv - Neuroscience 2024Quote: ... with the Lenti-X qRT-PCR Titration Kit (Takara Bio Inc., Kusatsu, Japan).
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bioRxiv - Neuroscience 2024Quote: ... 2 µg/mL doxycycline (Clontech; Cat. No. 631311), and 50 nM TMP (MP Biomedical ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody Rabbit-anti-DsRed (Clontech #632496, 1:1000) and secondary antibody HRP-goat-anti-rabbit (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... siRNA SMARTpool was mixed with pEGFP-N1 plasmid obtained from Clontech (Mountain View, CA, USA) at a final concentration of 50nM ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic titer was determined using a Lenti-X GoStix Plus kit (Takara). Lentivirus was functionally titrated previously using parallel preparation of GFP-expressing viral particles (Campeau et al. ...
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bioRxiv - Molecular Biology 2024Quote: RNA that passed the quality test underwent RNA polyadenylation and cDNA synthesis according to the instructions of the kit manufacturer (Mir-X™ miRNA First-Strand Synthesis Kit; Takara). The reaction system was configured according to the real-time fluorescence quantitative PCR user manual (ChamQ Universal SYBR® qPCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... 800 ng RNA was used with the SMARTer RACE 5’/3’ kit (Takara) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg RNase H-treated RNA was poly-A tailed with 2 U of poly(A) polymerase (Takara) for 1 hour at 37 ℃ ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloning reactions were performed at 50 °C for 1 h using the In-Fusion® HD Cloning Kit (Takara Bio). The reaction mixtures were further transformed into TG1 electrocompetent cells (Lucigen) ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was then synthesized using the SMART-seq HT kit (Takara Bio, Inc, Kusatsu, Japan); one sample (PV M ZT18 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were dissected on ice in RNAiso Plus (#9109, Takara Bio Inc.). Five repeats of pedicels and heads were collected ...
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bioRxiv - Neuroscience 2024Quote: Library preparation and RNA sequencing were performed by Novogene Co using the SMART-Seq v4 PLUS Kit (Takara Bio, Kusatsu, Shiga, Japan, # R400752). Total RNA was reverse transcribed into the first-strand cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in phosphate-buffered saline (PBS; #T9181, Takara) was placed onto the gap created by removing flagellum ...
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bioRxiv - Neuroscience 2024Quote: ... were obtained from Takara (2410A and 2250A). Hydroxylamine was from Aladdin Bio-Chem Technology Co. ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... strand cDNA was synthetized using the TaKaRa PrimeScipt RT Reagent Kit (Cat# RR037A, Takara Bio Inc., Kusatsu, Japan) using 20 µM Random decamer first strand primers according to the manufactorer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Apoptotic cells were quantified via the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay using the In situ Apoptosis Detection kit (Takara Bio). Visualization of apoptotic cells was performed using the Evos FL Cell Imaging System (Life Technologies).
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bioRxiv - Molecular Biology 2024Quote: ... The soluble proteins were purified using His60 Ni Superflow Resin (Takara). The purified proteins were further isolated using Superdex 75 16/600 (Cytiva) ...
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bioRxiv - Molecular Biology 2024Quote: The oligonucleotide library was amplified with a pair of primers using PrimeSTAR® Max DNA Polymerase (Takara Bio). The P8 and P3 phagemids were double-digested using NcoI-HF® (NEB ...
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bioRxiv - Molecular Biology 2024Quote: 2.5 mM dNTP mix (Takara, Cat#RR002M)
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bioRxiv - Molecular Biology 2024Quote: La Taq polymerase (Takara, Cat#RR002M)
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bioRxiv - Molecular Biology 2024Quote: 10x La Taq buffer (Takara, Cat#RR002M)
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bioRxiv - Biochemistry 2024Quote: ... The plasmid for GFP-β-actin expression was modified as a cDNA-encoding fluorescent tag of YFP-β-actin (TaKaRa-Clontech, Shiga, Japan) was substituted into that encoding eGFP (peGFP-actin).
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bioRxiv - Biochemistry 2024Quote: ... The plasmid for GFP-β-actin expression was modified as a cDNA-encoding fluorescent tag of YFP-β-actin (TaKaRa-Clontech, Shiga, Japan) was substituted into that encoding eGFP (peGFP-actin).
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bioRxiv - Biochemistry 2024Quote: ... removal of N-terminal signal peptide and introduction of mutation was performed based on the manufacturer’s instruction using PrimeSTAR MAX (Takara Bio, Shiga, Japan). The resulting plasmids were introduced into E ...
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bioRxiv - Biochemistry 2024Quote: ... anti-mCherry (#Z2496N, TaKaRa, Shiga, Japan) anti β-Actin (#bs-0061R ...
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bioRxiv - Biochemistry 2024Quote: ... TALON® metal affinity resin (500 μl, Takara Bio, Cat#635501) was equilibrated by resuspending the beads twice in 10 ml of binding buffer ...
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bioRxiv - Biochemistry 2024Quote: Anhydrotetracycline (aTc, TaKaRa, 631310), ATPγS (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... Arf1-EGFP and Arf1-mCherry plasmids were obtained by subcloning Arf1 (Wessels et al., 2006) into pEGFP-N3 (Clontech) and pmCherry-N1 (Clontech) ...
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bioRxiv - Biochemistry 2024Quote: ... YatalaseTM Enzyme (Takara Bio), and lyticase from Arthrobacter luteus (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: The PCR mixtures for the reactions described here were composed using PrimeStar (Takara Bio, Shiga, Japan) with a 2X GC buffer ...
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bioRxiv - Plant Biology 2024Quote: ... and 14-30 nt ssRNA Ladder Marker (Takara™, catalog no. 3416). Gels were stained with 1X SYBR Gold Nucleic Acid Gel Stain (Invitrogen™ ...
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bioRxiv - Plant Biology 2024Quote: ... with an In-Fusion Cloning Kit (Clontech Laboratories). Plasmid DNA was purified using a ZR Plasmid Miniprep-Classic Kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: ... The obtained cDNA was subjected to 5’ RACE-PCR using SeqAmp DNA Polymerase (Takara Bio USA). Primers used for 5’ RACE-PCR and the number of PCR cycles are shown in Supplemental Table 3 ...