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Citations for Takara Bio :
1951 - 2000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... using cDNA from adult zebrafish tissues as a template and cloned into the expression vector pCMV-HA or pCMV-Myc (Clontech) vectors ...
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bioRxiv - Immunology 2024Quote: ... activated CD8+ T cells were transduced with retrovirus expressing B7-H3-CAR on retronectin-coated (cat. T100B, Takara) non-tissue-culture-treated plate in complete RPMI medium supplemented with 50 U/ml rhIL-2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3′-RACE CDS primer (ClonTech) for cDNA synthesis from 5,4 μL uf viral RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and Premix Ex Taq master mixer (Takara).
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bioRxiv - Neuroscience 2024Quote: ... Full-length of itga2 was inserted into the Sfi IA (5′-GGCCATTACGGCC-3′) and Sfi IB (3′-GGCCGCCTCGGCC-5′) sites of the “prey” pPR3-C vector (Clontech). Series of combinations of bait and prey constructs were cotransformed into the yeast strain NMY51 (Clontech) ...
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bioRxiv - Neuroscience 2024Quote: ... Series of combinations of bait and prey constructs were cotransformed into the yeast strain NMY51 (Clontech), and different concentrations of bacterial liquid were growing onto SD/-Trp/-Leu plates for 3 d at 30°C ...
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bioRxiv - Neuroscience 2024Quote: The coding region of s1pr1 was fused to the GAL4 binding domain of the “bait” pBT3-STE vector (Clontech) cloning at Sfi IA (5′-GGCCATTACGGCC-3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatants containing the His-tagged fluorescent biosensors were purified with TALON Metal Affinity Resins (Takara Bio USA, Inc., California, USA) according to the manufacturer’s procedure ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was prepared with 750 ng of RNA using the Primescript RT kit (Takara Inc.) and analyzed with PerfeCa® SYBR® Green FAstMix® ROX (Quanta bio ...
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bioRxiv - Molecular Biology 2024Quote: ... KODone PCR master mix and In-Fusion Snap Assembly Master Mix were purchased from Takara Bio Inc ...
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bioRxiv - Molecular Biology 2024Quote: pUC19 purified by CsCl-EtBr ultracentrifugation was purchased from Takara-Bio ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were stained with primary antibodies for rat anti-CDH1 (M108, Takara Biosciences), rabbit anti-cytokeratin 8 (MA5-14476 ...
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bioRxiv - Molecular Biology 2024Quote: ... The ligated RNA was then subjected to TaqMan qPCR using the One Step PrimeScript RT-PCR Kit (Takara Bio), with 200 nM of a TaqMan probe targeting the boundary of the target RNA and the 3′-AD ...
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bioRxiv - Molecular Biology 2024Quote: ... while amplicons over 5 kb were produced using PrimeStar GXL polymerase (Takara Bio, San Jose, CA, #R050A) in a two-step amplification reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... Residual genomic and plasmidic DNA was removed using DNA-free kit (InvitrogenTM) and 500 ng of RNA were retro-transcribed with PrimeScript RT Master Mix (Takara Bio) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using sequential In-Fusion Cloning (Takara Bio) reactions ...
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bioRxiv - Molecular Biology 2024Quote: ... was PCR-amplified from SK-N-BE cells cDNA and cloned between the ICSs using In-fusion Cloning Kit (Takara Bio). 4xPepper array sequence (196 bp long ...
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bioRxiv - Molecular Biology 2024Quote: ... CloneAmp HiFi PCR Premix (Clontech) was used for all the PCR amplifications.
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 100ng/mL Doxycycline and 1µM Shield1 (Clontech Takara) for 15h to induce the expression of Dam-fused proteins ...
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bioRxiv - Molecular Biology 2024Quote: Strand-specific RNA-seq was performed on parental and ibrutinib-resistant Rec-1 cells using SMARTer Stranded Total RNA Sample Prep Kit (Takara, cat# 634873) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were created using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio Inc.). DNA samples were repaired ...
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bioRxiv - Molecular Biology 2024Quote: ... The incubated DNA samples were purified with the NucleoSpin Gel and PCR Clean-up kit (#740609.250; Takara Bio Inc.). The purified DNA samples were used to prepare the library ...
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bioRxiv - Molecular Biology 2024Quote: ... and the insert fragments fused to the linearized plasmids using the In-Fusion Cloning Kit (Takara Bio Inc., Shiga, Japan), according to the protocol provided by the manufacturer with the Escherichia coli JM109 strain ...
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bioRxiv - Microbiology 2024Quote: ... The amplified linear fragment of pKNOCK-Km and the internal fragments of each gene were fused using In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and SacI (Takara Bio), and circularized DNA prepared by self-ligation with Ligation high Ver.2 (Toyobo ...
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bioRxiv - Microbiology 2024Quote: ... pRF vectors with chimeric L-segments were constructed by in-fusion high-fidelity (HD) restriction-free cloning method (TaKaRa Bio). Two nucleotides of LF2384 L gene were different from those of parent wildtype virus and one nucleotide of LF2350 GPC gene of was different from that of wildtype one ...
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bioRxiv - Microbiology 2024Quote: ... One microgram of total RNA was used for reverse transcription with Revert Aid™ Reverse Transcriptase (TaKaRa, Tokyo, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Total RNA was isolated from larval zebrafish or liver /spleen /kidney tissues of adult zebrafish with Trizol reagent (TaKaRa, Tokyo, Japan) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... vectors using In-Fusion cloning (Takara). HA-RelA was codon-optimized and synthesized as dsDNA fragment (IDT ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian” kit (Takara Bio USA, Cat. # 634411), following “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian User Manual (Rev ...
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bioRxiv - Microbiology 2024Quote: ... described below) were constructed via FastCloning (63) or with the In-Fusion HD Cloning Kit (Takara). Primers used in this study are listed in Table S4 ...
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bioRxiv - Microbiology 2024Quote: The purified product in ICDS method and captured beads in upgraded ICDS method were ready for Illumina library preparation with SMART ChIp-seq kit (Takara, Catalog # 634865) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... cDNA transcribed from 0.025 μg total RNA was used per PCR reaction in a final volume of 20 μl with TB Green Premix Ex Taq II (#RR820A, Takara Bio). PCR was performed using a Biorad CFX96 Touch Real-Time PCR Detection System ...
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bioRxiv - Molecular Biology 2024Quote: PCR-amplified fragments were cloned into pINDUCER20 using a ligation-independent cloning protocol with 5x In-Fusion HD Enzyme Premix (Takara Bio USA, Inc.) per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... was concentrated using the LentiX Concentrator reagent (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was extracted from cells using the NucleoSpin RNA Plus Kit (Clontech, 740984.250). Following RNA extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by incubation with proteinase K (Takara) for 2 h at 55 °C ...
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bioRxiv - Neuroscience 2024Quote: ... PCR-amplified and gel-purified Homology Box A and iCre-P2A-TdTomato were inserted into iTV vector by In-Fusion (Clontech) and transformed into DH10B bacteria ...
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bioRxiv - Neuroscience 2024Quote: ... immediately followed by reverse-transcriptions and cDNA amplification with the smart-seq HT kit (Cat# 634437, Takara). The post-amplified cDNAs (1 ng per sample ...
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bioRxiv - Microbiology 2024Quote: ... then introduced between BamHI and EcoRI sites of pRetroX-Tight- Pur vector (Takara, 632105). Resulting plasmids containing the desired mutation were confirmed by DNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... with the One Step PrimeScript™ RT-PCR Kit (Takara Bio, RR064A). The following primers targeting a 244-base amplicon of the IAV M segment were used:
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 100ng/mL Doxycycline and 1µM Shield1 (Clontech Takara) for 15h to induce the expression of Dam-fused proteins ...
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bioRxiv - Microbiology 2024Quote: ... or the empty vector as control (Takara, 632105). 48 h after transfection ...
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bioRxiv - Microbiology 2024Quote: ... then introduced between XhoI and AgeI sites of pCMV-FLAG vector (Takara, 632105). Resulting plasmids containing the desired mutation were confirmed by DNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... 2 U/µl of Ribonuclease Inhibitor (Takara Inc), 20 ng/µl of plasmid DNA ...
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bioRxiv - Microbiology 2024Quote: ... and all gene cloning operations were performed using an In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan). A conjugated pZJD29a vector [65] was employed to clone 500-bp upstream and downstream regions of the target gene ...
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bioRxiv - Microbiology 2024Quote: ... Yeast cells containing pGBKT7PA28γ were grown in yeast extract-peptone-dextrose medium and transfected with a human foetal brain plasmid library based on pACT2 (Clontech Takara). Clones (22.4 × 106 ...
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bioRxiv - Neuroscience 2024Quote: ... Detailed procedures were conducted following the manufacturer’ s instructions (Clontech).
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bioRxiv - Molecular Biology 2024Quote: The isolated RNAs were subsequently reverse transcribed using random hexamer primers and SMARTScribe Reverse Transcriptase (Clontech; 639537). Indicated genes were analyzed by RT-qPCR using Fast SYBR Green Master Mix (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... and concentrated 1:10 with Lenti-X concentrator (Takara Bio).