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Citations for Takara Bio :
1901 - 1950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral supernatants generated in Lenti-x HEK-293T cells (TAKARA, #632180) using the 2nd generation packaging system (plasmids psPAX2 ...
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bioRxiv - Cell Biology 2024Quote: ... Human CXCR4 was cloned into the pAcGFPm-N1 plasmid (Clontech Laboratories, Palo Alto, CA), as described (20).
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bioRxiv - Biophysics 2024Quote: ... were generated using In-Fusion HD Cloning kit (Clontech (TaKaRA)) following the supplier’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... were generated using In-Fusion HD Cloning kit (Clontech (TaKaRA)) following the supplier’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The In-Fusion cloning kit (Takara Bio) was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA from the same amount of RNA between RNAi and control samples were synthesized in a 20 μl reaction volume by the PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA, Code No. RR047A). One μl of cDNA was used as the template in a 10 μl reaction volume of the PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... templates were constructed by cloning the 1.5 kb upstream and downstream homology arms into the pPD95.77 plasmid using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). Fluorescence tags were inserted into the constructs with a flexible linker before the stop codons ...
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bioRxiv - Cell Biology 2024Quote: ... LX-293T cells (Takara) were seeded into 6-well plates and grown till 80% confluence was reached ...
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bioRxiv - Cell Biology 2024Quote: ... (2014)) to compute the log fold change between the known frequencies of the guides in the library (Takara) and the actual observed frequencies of the guides (computed by pooling all bins together) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were grown for 72 hours post-transfection and the virus was concentrated ∼40-fold from the supernatant using the Lenti-X concentrator kit (Takara) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The two best performing guides from the genome-wide screen (Table S3) were selected and synthesized (IDT) and then cloned into the pLVXS-sgRNA-mCherry-hyg Vector (Takara) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-PCR was performed using diluted cDNA (1:5 in water) and PrimeStar DNA Polymerase (Takara, R010A) with primers and PCR conditions listed in Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: 3’ and 5’RACE reactions were carried out using the SMARTer 3’5’ RACE kit (Takara), essentially as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... The non-solubilized fraction was separated by centrifugation at 47850 x g for 1 h at 4 °C and 4 mL of cobalt-charged TALON resin (Clontech, Takara) was added to the solubilized fraction for batch binding (3 h at 4 °C) ...
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bioRxiv - Neuroscience 2024Quote: ... The non-solubilized fraction was separated by centrifugation at 47850 x g for 1 h at 4 °C and 4 mL of cobalt-charged TALON resin (Clontech, Takara) was added to the solubilized fraction for batch binding (3 h at 4 °C) ...
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bioRxiv - Plant Biology 2024Quote: ... and first strand cDNA synthesis were performed using gDNA Eraser (Perfect Real Time) and PrimeScript Reverse Transcriptase following manufacturer’s instructions (Takara Bio Inc. Kusatsu, Japan). For gene expression analysis ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with either rapalog A/C heterodimerizer (final concentration 5μM; Takara, Cat# AP21967) or 200 μL ethanol (control) ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression levels were determined using RT-qPCR performed on a Thermal Cycler Dice Real Time System Single TP850 (Takara Bio Inc.) using THUNDERBIRD® SYBR® qPCR Mix (QPS-201 ...
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bioRxiv - Genetics 2024Quote: ... Yeast transformation was carried out using the Yeastmaker™ Yeast Transformation System 2 (Takara Bio Inc., cat. # 630439).
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bioRxiv - Genetics 2024Quote: ... esrp1 and esrp2 were each cloned into a pCS2+8 plasmid backbone using the In-Fusion HD Cloning Kit (Clontech). The resulting pCS2+8-esrp2 plasmid was mutagenized with synonymous mutations surrounding the translational start-site using the GeneArt site-directed mutagenesis (SDM ...
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bioRxiv - Genetics 2024Quote: ... corresponding to predicted CNL and NL protein sequences were synthesized by General Biosystems (Durham, NC, United States) and subsequently used for PCR amplification with PrimeSTAR® Max DNA Polymerase assay (Takara Bio Inc., cat. # R045A) for cloning into pGBKT7 DNA-BD and pGADT7 AD vectors (Table S6) ...
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bioRxiv - Developmental Biology 2024Quote: ... and 5 µl of SYBR® Premix-Ex-Taq (Tli RNase H Plus, Takara) in a 10 µl reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... The CDs of amd1 and skp2 were amplified individually using PCR (Prim STAR Max Premix Takara, R045A) and cloned into the PCS2+ vector (5x In-Fusion HD Enzemy Premix ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were harvested by scraping colonies from transformation plates and directly purifying plasmids (with no outgrowth that could introduce amplification bias) using Nucleobond Xtra midiprep kits (Takara).
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bioRxiv - Genetics 2024Quote: ... 1–1249 (full-length) were cloned into an EGFPC1 plasmid (N-terminal GFP tag) (Takara Bio, CA). For non-cleavable INF2 ...
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bioRxiv - Immunology 2024Quote: ... transformed into Stellar competent cells (Clontech) and selected by kanamycin.
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bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with the aforementioned pX330 vectors together with pEGFP-C1 (#6084-1, Clontech Laboratories, Mountain View, CA, USA) and cultured for 2 d ...
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bioRxiv - Cell Biology 2024Quote: ... GFP (Takara, # 632569, 1:1000) EGFR (Fitzgerald ...
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bioRxiv - Cell Biology 2024Quote: ... Doxycycline (Clontech, #631311) was used at 100 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral packaging was produced in HEK293T cells transfected with pCAG-HIVgp and pCMV-VSV-G-RSV-Rev packaging plasmids (provided by RIKEN BRC DNA Bank) via TransIT-2020 (TaKaRa). In order to conduct lentiviral infection ...
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bioRxiv - Biochemistry 2024Quote: ... and pmCherry-N1 (Clontech), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... tue1 lacking the signal peptide was inserted into pGILDA (Clontech) to generate a fusion with the lexA gene ...
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bioRxiv - Cell Biology 2024Quote: ... pyogenes Cas9-tagBFP expressing HL-60 cells (Graziano et al. (2019)) were transduced by spinoculating the cells on Retronectin-coated (Takara) non-TC treated 6-well plates (Falcon #351146) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of RNA was reverse transcribed using PrimeScriptTM RT reagent Kit (Takara, #RR047A). Real-time quantitative PCR was performed using SYBR Green Mix (Abclonal ...
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bioRxiv - Cell Biology 2024Quote: ... Using Lenti GoStix (Takara), we estimated that this corresponds to 8×106 IFUs per well ...
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bioRxiv - Molecular Biology 2024Quote: ... or the pIExZ vector using an In-Fusion cloning kit (Takara). pCold-DDX43 ...
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bioRxiv - Immunology 2024Quote: ... Total RNA Sequencing libraries were prepared with SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The isolated RNA was reverse transcribed to cDNA following the manufacturer’s protocol (PrimeScript™ RT Master Mix, TaKaRa). The mRNA levels of RUNX2 (Hs00231692_m1) ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was isolated through a NucleoSpin® RNA/Protein kit (Takara Bio USA Inc. San Jose, CA) while heart issue was processed with a RNeasy Fibrous Mini kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... with the TB Green Premix Ex Taq PCR master mix (Takara). For each sample ...
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bioRxiv - Neuroscience 2024Quote: ... Viral supernatants were then concentrated using Lenti-X Concentrator (Takara) and spun for 45 min at 1500 x g before resuspending in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... or 10 µg DsRedExpress (Clontech) together with 1-2 µg of GFP-tagged PSD95α41 or PSD93α42 ...
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bioRxiv - Molecular Biology 2024Quote: ... dsDNA templates were synthesized using PrimeSTAR GXL DNA Polymerase (Takara Bio) and the primers shown in Table S4 ...
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bioRxiv - Molecular Biology 2024Quote: ... purified using Lenti-X Concentrator (Takara #631231), and concentrated into 200 μL of OptiMEM (eg Gibco #31985062) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3′-RACE CDS primer (ClonTech) for cDNA synthesis from 5,4 μL uf viral RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and Premix Ex Taq master mixer (Takara).
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bioRxiv - Molecular Biology 2024Quote: pUC19 purified by CsCl-EtBr ultracentrifugation was purchased from Takara-Bio ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were stained with primary antibodies for rat anti-CDH1 (M108, Takara Biosciences), rabbit anti-cytokeratin 8 (MA5-14476 ...
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bioRxiv - Molecular Biology 2024Quote: ... The ligated RNA was then subjected to TaqMan qPCR using the One Step PrimeScript RT-PCR Kit (Takara Bio), with 200 nM of a TaqMan probe targeting the boundary of the target RNA and the 3′-AD ...
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bioRxiv - Molecular Biology 2024Quote: ... while amplicons over 5 kb were produced using PrimeStar GXL polymerase (Takara Bio, San Jose, CA, #R050A) in a two-step amplification reaction ...