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Citations for Takara Bio :
1851 - 1900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... membranes were incubated with mouse anti-GFP (Clontech #632380), rabbit anti-DsRed (Takara #632496) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from pre-metastatic (0 hrs APF) and post-metastatic (24 hrs APF) Scrib knockdown pupae using TRI reagent (TAKARA) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... AML cells and lentiviral particles were co-cultured at a seeding density of 1×106 cells per mL in a Retronectin dish (Takara Bio; T110A). Following 72 hours of infection ...
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bioRxiv - Cancer Biology 2024Quote: ... and primary antibodies STEM121 (1:500) (Takara #Y40410), ANXA1 (1:400) ...
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bioRxiv - Cancer Biology 2024Quote: ... Vectors were amplified in Stellar Competent Cells (Takara Bio) and respective integrated shRNA was verified by Sanger sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... using In-Fusion HD Cloning Kit (Takara Bio, USA). Vectors were amplified in Stellar Competent Cells (Takara Bio ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for S100a9 with a primer set (MA058882) obtained from TaKaRa Bio Inc ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... SYBR Premix Ex Taq Perfect Real Time (TaKaRa Bio Inc.) for S100a9 with a primer set (MA058882 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and Premix Ex Taq Perfect Real Time (TaKaRa Bio Inc.) for 18S rRNA with TaqMan Ribosomal RNA Control Reagents (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... 94 The plasmids pME-rtTA and p3E-TRE were made by inserting the Tet-On fragments from the Tet-On system (Clontech) in the appropriate Tol2 entry vectors ...
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bioRxiv - Cell Biology 2024Quote: cfDNA in the conditioned cell culture medium was extracted using NucleoSpin Gel and PCR Clean-up kit (Takara, Duren, Germany), and quantified with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... using an In-Fusion cloning kit (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... pCold-DDX6 A DNA fragment encoding DDX43 or DDX6 was amplified by PCR and inserted into the pCold-I vector (Takara) using an In-Fusion cloning kit (Takara).
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bioRxiv - Immunology 2024Quote: ... cDNA generation (SMART®-Seq v4 Ultra Low Input RNA Kit, Takara Bio) and library preparation (Nextera® XT DNA Library Prep Kit ...
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bioRxiv - Genomics 2024Quote: ... or a Takara NucleoSpin RNA Plus kit (Takara, 740984) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Tissue samples were homogenized using a sterile Takara BioMasher (Takara, 9791A). RNA isolation was performed using either a Qiagen RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... and spleen tissues using an RNAiso Plus kit (Takara Bio), and subsequently reverse transcribed into cDNAs ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA from flash-frozen cells was processed using NucleoMag (744350.1, Takara) Magnetic bead-based RNA isolation following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: TUNEL staining was performed using the In Situ Apoptosis Detection Kit (TAKARA BIO INC., Japan) with minor modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... and libraries were prepared with the SMARTer® smRNA-Seq Kit for Illumina (Takara Bio) to selectively capture transcripts <150nts.
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from Mm cells using the TRIzol Reagent (Takara, Japan). cDNA was synthesized using a PrimeScript reverse transcriptase reagent kit (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... using In-Fusion cloning kit (Takara). Truncated versions of FLAG::TurboID::SQSTM1 were generated by PCR using primers corresponding to the truncated SQSTM1 gene and amplification using Q5 high-fidelity (M0492 ...
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bioRxiv - Cancer Biology 2024Quote: Phoenix-ECO and Lenti-X packaging cells (Takara) were cultured in DMEM medium (Thermo Fisher Scientific ...
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6-Phosphogluconolactonase is critical for the efficient functioning of the pentose phosphate pathwaybioRxiv - Biochemistry 2024Quote: ... coli BW25113 WT strain by PCR and inserted in pZA23 plasmid using the In-Fusion® HD Cloning Plus CE Kit (Takara).
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bioRxiv - Immunology 2024Quote: ... The membrane was developed with ECL reagent (Takara). Whole cell lysates of Th2 cells were prepared and immunoprecipitated with 2 µg of pulldown antibodies (Gata3 ...
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bioRxiv - Immunology 2024Quote: Dual-luciferase reporter assay was performed to assess regulatory functions of Il4 and Il13 promoters along with the Ecr region (previously described in cloning section). HEK293T cells (kind gift from Dr. Arnab Gupta, IISER Kolkata) were transfected (Xfect transfection reagent, Takara, Kusatsu, Japan) with p4-pGL3 ...
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bioRxiv - Neuroscience 2024Quote: ... and 2 μg/ml doxycycline (Clontech, 631311). Neuronal induction media was changed once a day for 2 more days ...
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bioRxiv - Cell Biology 2024Quote: ... Human Sec24B was subcloned into pmCherry-C1 (Clontech) using SalI and BglII restriction sites and verified by sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription to cDNA was performed with PrimeScript™ RT Master Mix (Takara Bio #RR036B). RT-PCR was then performed with SsoAdvanced Universal SYBR (Bio-Rad #1726275 ...
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bioRxiv - Cancer Biology 2024Quote: ... Synthesis of cDNA was performed using a PrimeScript™ RT reagent kit (TaKaRa Bio; Tokyo, Japan), and PCR was performed with Taq DNA Polymerase (Toyobo ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR was performed on the Thermal Cycler Dice® Real Time System III instrument (TaKaRa Bio; Tokyo, Japan), The reaction was performed as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis kit (6210A) and SYBRmix (RR820) were purchased from TAKARA. ATM inhibitor KU-55933 (SML1109) ...
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bioRxiv - Cancer Biology 2024Quote: ... and transgene plasmids were transfected into HEK293FT cells to generate lentivirus media as previously described (33) and concentrated using Lenti-X concentrator (Takara Bio #631232) according to the manufacturers protocol.
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were then ligated in then In-Fusion® reaction (Takara). Positive colonies were confirmed by Sanger sequencing (Source Bioscience ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated overnight with 1 mL of Talon (immobilized metal affinity chromatography, IMAC) resin (Takara) in the presence of 10 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... Interferon expressions were measured using TB Green® Premix Ex Taq™ (Tli RNaseH Plus) reagent (Takara) on a QuantStudio™ 6 Flex (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: Promoters of genes of interest were cloned from the genomic DNA of MDA-MB-231 cells and cloned into an EcoRI-linearized luciferase reporter vector using In-Fusion cloning (Takara Bio cat. 638948). Lentivirus was packaged using Lipofectamine 3000 following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... using In-Fusion cloning (TaKaRa Bio, 638948). During In-Fusion cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... and was reverse transcribed to make amplified cDNA by SMART seq v4 ultra-low input RNA kit (Takara Bio Inc, USA). After shearing of cDNA by Covaris ultrasound water bath sonicator and purification by Ampure beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... we employed SMART-seq technology (Takara Biosciences) which can amplify cDNA directly from 1-1000 cells ...
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bioRxiv - Cancer Biology 2024Quote: cDNA was amplified using SMART seq v4 ultra-low input RNA kit (Takara Biosciences). 0.5 ng of amplified cDNA from EV-H and EV-C RNA was used as template ...
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bioRxiv - Cancer Biology 2024Quote: ... restriction enzymes followed by In-Fusion Snap Assembly (Takara, #638947) using the manufactures’ directions ...
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bioRxiv - Cancer Biology 2024Quote: ... The wild-type AKT1 (NM_001014431.2) was cloned in the pECMV-3×FLAG-N vector using the In-Fusion® HD Cloning Kit (Takara Bio, Cat# 639650). The K20R and K20Q mutant AKT1 plasmids were constructed using the Fast Site-Directed Mutagenesis Kit (TIANGEN ...
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bioRxiv - Cancer Biology 2024Quote: ... Pico Input Mammalian (Takara Bio). All the libraries were single-end sequenced (1×88 bp ...
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bioRxiv - Developmental Biology 2024Quote: ... transversa using the SMARTer RACE cDNA Amplification kit (Clontech). For each transcript ...
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bioRxiv - Cell Biology 2024Quote: ... with the pRetroX construct and pVSV-G (Clontech) using Lipofectamine LTX reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... or a mutant p53 (p53 QM) were generated by infection of H1299 cells with the corresponding retrovirus and the rtTA retrovirus (Clontech), followed by selection with puromycin (1 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and pDONR22-KRT6A (DNASU clone HsCD00039474) and inserted into pLVX-IRES-Puro or pLVX-IRES-Neo lentiviral expression vectors (Clontech) with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix ...
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bioRxiv - Cell Biology 2024Quote: ... EGFP from pEGFP-C1 (Clontech) was inserted into pLVX-IRES-Puro using the SnaBI and BamHI restriction sites.
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR (qPCR) was conducted in technical duplicate samples utilizing TB Green Premix Ex Taq II (Takara, Cat# RR820S) and the Thermal Cycler Dice (Takara ...