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Citations for Takara Bio :
1801 - 1850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Immunoreactive bands were luminescent in a chemiluminescent substrate (Western BLoT Quant HRP substrate, cat. # T7102A, TaKaRa) and imaged using an image analyzer (LAS4000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Dync2li1-shRNA and Control-shRNA cassettes were subcloned into the pBAsi-mU6 vector (Takara Bio, Shiga, Japan). The target sequences were as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... 33 μg/mL retronectin (Clontech) and 2.67 μg/mL of DL1-extracellular domain fused to human IgG1 Fc protein (a gift from I ...
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bioRxiv - Immunology 2024Quote: ... following the manufacturer’s protocol for in-fusion cloning (Takara Bio). All heavy chains were similarly cloned into IgG1 and IgM plasmids in parallel ...
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bioRxiv - Microbiology 2024Quote: ... cDNA levels of the phoP and eptB genes were quantified by PCR using SYBR Premix Ex Taq II (Takara, Japan) solution containing RT-PCR primers for each gene (See Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... All RNAs in the samples were converted into cDNA using a cDNA EcoDry Premix (Clontech, USA). cDNA levels of the phoP and eptB genes were quantified by PCR using SYBR Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using the PrimeScript RT-PCR kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using TB Green Premix Ex Taq Kit (Takara) on QuantStudio 6 Flex Real-Time PCR System (Life Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... digested pLG plasmid by In-Fusion® HD Cloning Kit (Takara) resulting in the pLd2G vector ...
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bioRxiv - Biochemistry 2024Quote: A pull-down assay was performed using a Capturem His-Tagged Purification Miniprep Kit (#635710, Takara, Shiga, Japan). 50 µg of BSA was added to the column for blocking ...
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bioRxiv - Developmental Biology 2024Quote: ... [61] digested with EcoRI/XhoI by In-Fusion HD Cloning Kit (Takara, 639649). Twenty-four hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... a PGK promoter-driven mCherry expression cassette and the WPRE element were inserted into pMSCV (Clontech, Takara Bio) at the XhoI and ClaI sites to generate an empty vector (pMSCV-mCherry-WPRE) ...
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bioRxiv - Neuroscience 2024Quote: Genomic DNA was extracted from 10 whole bodies of wandering flies using MightyPrep reagent for DNA (Takara #9182). Whole body RNA was extracted from 10 whole bodies of wandering flies using TRIzol (Ambion #15596018) ...
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bioRxiv - Cell Biology 2024Quote: ... Both cDNAs were cloned into pIRES (Clontech) for their coupled expression from bicistronic mRNA to achieve near-quantitative Shh palmitoylation 23 ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA synthesis was performed using PrimeScript 1st strand cDNA Synthesis Kit (TakaRa, 28704) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µL of the eluate was digested overnight at 37°C with 10 units of DpnI (Takara, 1235A) in a final volume of 10 µL ...
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bioRxiv - Microbiology 2024Quote: Total RNA was isolated at specific time points post infection by using TRIzol (Takara) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... RNA was reverse transcribed using RNA to cDNA Ecodry premix (Takara Bio, Kusatsu, Japan). Gene expression was measured using Power SYBR Green PCR Master mix (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... using SMARTer Ultra Low Input kit (634940, Takara Bio/Clontech). All samples were sequenced in parallel at two different time points using Illumina NovaSeq 6000 flow cell and fastq files have been deposited on the Gene Expression Omnibus (GEO ...
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bioRxiv - Microbiology 2024Quote: ... 5 U/µL Ex Taq polymerase (Takara Bio, Kusatsu, Shiga, Japan), 20 mg/mL BSA (Roche Molecular Diagnostics ...
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bioRxiv - Biochemistry 2024Quote: ... a 125 μl Talon (Takara, 635501) resin bed was used ...
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bioRxiv - Neuroscience 2024Quote: ... The standard curve was prepared using the positive control solution provided by Takara with serial dilution for absolute quantification ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA cloning libraries were created using SMARTScribe™ Reverse Transcriptase (Takara/Clontech Cat # 639537) and the Advantage 2 PCR kit (Takara/Clontech Cat # 639207) ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with 10% blocking buffer (Nacalai Tesque, 03953-95) prepared in Tris-buffered saline/Tween 20 (Takara Bio, T9142) (TBS-T ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR analysis was then performed with 1.0 μl of 5-fold diluted cDNA using a SYBR premixed Ex Taq kit (Takara), and a Light Cycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Plant Biology 2024Quote: The full-length coding sequences (CDS) of NPR1 and TGA were fused to the bait vector pGBKT7 (Clontech, Palo Alto, CA, USA) by NdeI (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-dsRed (1:1,000, Clontech 632496), donkey anti-chicken Cy2 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Virus-rich supernatants were concentrated using Lenti- X™ Concentrator (Takara Bio, 631231) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), the trans-plasmid providing viral capsid PHP.eB (a generous gift from Viviana Gradinaru ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Neuroscience 2024Quote: ... SMARTScribe Reverse Transcriptase kit (Clontech) was used to generate cDNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... and 19 µL 10X lysis buffer (TaKaRa, Cat# 635013) diluted to 1X with nuclease-free water ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysis buffer was prepared by mixing 1 µL RNase inhibitor (TaKaRa, Cat# 2313A) and 19 µL 10X lysis buffer (TaKaRa ...
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bioRxiv - Genomics 2024Quote: ... was transfected into HEK293T cells using the Lenti-X Packaging Single Shots (Takara Bio 631275), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Neuroscience 2024Quote: ... plasmids were generated by PCR amplification of HTTex1 cDNAs and subcloning to replace the CD4 sequence in the pUASTattB(MApHS) plasmid (Han et al., 2014) via In-Fusion cloning (Takara Bio USA, Inc., Mountain View, CA). pUASTattB(mCherry-Galectin-3 ...
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bioRxiv - Neuroscience 2024Quote: ... using 10 ng of input RNA per replicate and TB Green Premix Ex Taq II (Takara Bio, Kusatsu, Japan). Sequences of all qPCR primers used in this study are listed in Table 3.
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bioRxiv - Molecular Biology 2024Quote: ... AAV titer was determined by qPCR method using AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara-bio, #6233) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was performed using PrimeScript (Takara, Cat# RR036A) to synthesize cDNA from total RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and the pRep/Cap 1A-MYO capsid vector 56 into AAVpro 293T cells (TaKaRa, 632273) using polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2024Quote: ... and the Thermal Cycler Dice (Takara, Cat# TP850). The expression levels of the target genes were normalized to that of Rpl13a ...
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bioRxiv - Molecular Biology 2024Quote: ... Co-transfection with the pEGFP-N2 plasmid (Takara Bio, CA, USA) served as a control for different transfection efficiencies ...
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bioRxiv - Neuroscience 2024Quote: ... pLV-hsynapsin-CTNND2-myc-WPRE and pLV-hsynapsin-SRGAP2C-HA-myc-WPRE were generated by insertion of the cDNAs at a multiple cloning site (MCS) of a pLV-hsynapsin-MCS-WPRE plasmid (described in24) using InFusion cloning (Clontech, Cat#638909).
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bioRxiv - Neuroscience 2024Quote: ... Titration of the concentrated viruses was performed by AAVpro Titration Kit (for Real Time PCR) Ver.2 (6233, Takara Bio Inc) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (Takara #632496), mouse anti-Actin (Sigma #A4700 ...
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bioRxiv - Neuroscience 2024Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), the trans-plasmid providing viral capsid PHP.eB (a generous gift from Viviana Gradinaru ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Microbiology 2024Quote: ... dNTPs and reverse transcriptase (Takara) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The expression profile of target gene was evaluated using specific primers (Table-3) by using SYBR green RT-PCR master mix (Takara) in BioRad Real time PCR instrument ...
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bioRxiv - Genomics 2024Quote: ... Viral titers were determined using the Lenti-X GoStix Plus (Takara Bio 631280). Viral infections were conducted with a MOI (multiplicity of infection ...