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Citations for Takara Bio :
1701 - 1750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... PCR amplification of sgRNA inserts was performed using Ex Taq DNA Polymerase (Takara) with a total of 54 μg of input genomic DNA to maintain 300X representation ...
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bioRxiv - Cancer Biology 2024Quote: ... This fragment was cloned into the pLVX-EF1α-IRES-Puro vector (Clontech, #631988) by first treating with the EcoR1-HF (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with Shield-1 (Takara, 632188) at a concentration of 1 µM for 4 days ...
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bioRxiv - Cancer Biology 2024Quote: ... The filtered virus-containing suspension was directly used for transduction or concentrated using Lenti-X concentrator (Takara, 631231) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... PGK-GFP was made by swapping the CMV promoter in CMV-EGFP-C1 (Clontech) vector with the hPGK promoter amplified from pLenti.PGK.blast-Renilla_Luciferase (Addgene 74444 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed to cDNA using SMART MMLV reverse transcriptase (Takara) and Oligo(dT)20 as a primer ...
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bioRxiv - Cancer Biology 2024Quote: ... 100436867-100437297: GRCh37/hg19) and either the wild-type or mutated PAS were cloned into the pEGFP-N3 plasmid (Clontech) at the BsrGI and NotI sites ...
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bioRxiv - Cancer Biology 2024Quote: ... Viral supernatant was collected 2 days post-transfection and concentrated using Lenti-X lentivirus concentrator (Clontech). Cells were then infected with the concentrated lentivirus ...
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bioRxiv - Cell Biology 2024Quote: ... followed by ligation using in-Fusion cloning enzymes (Takara Bio, 638948). hPRDX6 mutants (S32A ...
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bioRxiv - Cancer Biology 2024Quote: ... using Xfect Transfection Reagent (Takara, 631318) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Programed cell death was induced by incubating cells in complete DMEM with 1 mM B/B homodimerizer (Clontech) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were prepped using the NucleoBond Xtra Midi Plus kit (Takara Bio).
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bioRxiv - Cancer Biology 2024Quote: ... T cells were then plated on retronectin coated plates (Takara, T100A), stimulated with Human CD3/CD28 T Cell Activator (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T-Lenti-X cells (Takara Bio) were thawed ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody Rabbit-anti-DsRed (Clontech #632496, 1:1000) and secondary antibody HRP-goat-anti-rabbit (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... Viral supernatants were concentrated using Lenti-X Concentrator (Takara) and spun for 45 minutes at 1500 x g ...
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bioRxiv - Neuroscience 2024Quote: ... with the Lenti-X qRT-PCR Titration Kit (Takara Bio Inc., Kusatsu, Japan).
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bioRxiv - Neuroscience 2024Quote: ... 2 µg/mL doxycycline (Clontech; Cat. No. 631311), and 50 nM TMP (MP Biomedical ...
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bioRxiv - Neuroscience 2024Quote: ... siRNA SMARTpool was mixed with pEGFP-N1 plasmid obtained from Clontech (Mountain View, CA, USA) at a final concentration of 50nM ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was then synthesized using the SMART-seq HT kit (Takara Bio, Inc, Kusatsu, Japan); one sample (PV M ZT18 ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... strand cDNA was synthetized using the TaKaRa PrimeScipt RT Reagent Kit (Cat# RR037A, Takara Bio Inc., Kusatsu, Japan) using 20 µM Random decamer first strand primers according to the manufactorer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Tissues were dissected on ice in RNAiso Plus (#9109, Takara Bio Inc.). Five repeats of pedicels and heads were collected ...
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bioRxiv - Neuroscience 2024Quote: Library preparation and RNA sequencing were performed by Novogene Co using the SMART-Seq v4 PLUS Kit (Takara Bio, Kusatsu, Shiga, Japan, # R400752). Total RNA was reverse transcribed into the first-strand cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in phosphate-buffered saline (PBS; #T9181, Takara) was placed onto the gap created by removing flagellum ...
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bioRxiv - Neuroscience 2024Quote: ... were obtained from Takara (2410A and 2250A). Hydroxylamine was from Aladdin Bio-Chem Technology Co. ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies included rabbit anti-DsRed (Takara Bio, catalog #632496, 1:1000) and chicken anti-NeuN/FOX3 (EnCor ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellartis human iPSC line 12 (ChiPSC12, #Y00280) was purchased from Takara Bio ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant with the protein was then separated by ultracentrifugation (60,000 rpm, 45 min, 4°C) and incubated overnight with Talon metal affinity resin (Takara Bio). The protein was affinity purified from the solubilized fraction in a gravity column (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... The In-Fusion Snap Assembly (Takara Bio) was used as the standard method for cloning according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... coli strain BL21 (DE3) (NIPPON GENE) co-transformed with a pGro7 chaperone plasmid (Takara Bio) and purified as follows ...
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bioRxiv - Bioengineering 2024Quote: ... Virus was concentrated using Retro-X Concentrator (Takara, 631456). The following day ...
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bioRxiv - Bioengineering 2024Quote: ... Bead-stimulated cells were transferred to RetroNectin-coated (Takara) virus–containing plates and incubated overnight ...
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bioRxiv - Cancer Biology 2024Quote: TET-ON-Cas9-inducible expressing cells were generated firstly by infection with the rtTA plasmid pLVX-Tet3G (Takara, 631358) and neomycin selection ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by extraction with Trizol Reagent (Takara, Dalian, China) to obtain RNA.
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bioRxiv - Bioengineering 2024Quote: ... We confirmed the cells were mycoplasma-negative using a Cycleave PCR Mycoplasma Detection Kit (Takara Bio, CY232).
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bioRxiv - Bioengineering 2024Quote: ... The 500bp PCR fragments were purified by DNA electrophoresis and gel extraction(TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0) ...
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bioRxiv - Bioengineering 2024Quote: ... Linear DNA fragments were produced by PCR using HiFi polymerase (Takara, 639298), followed by treatment with DpnI to remove template DNA ...
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bioRxiv - Bioengineering 2024Quote: ... Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent Escherichia coli (Takara Bio, 636766). Plasmids were purified by miniprep (QIAGEN ...
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bioRxiv - Biophysics 2024Quote: ... wild-type (WT) MDCK cells were transfected with pAcGFP1-Mem vector (Takara, 632509). To decouple the LINC complex by overexpressing dominant-negative KASH protein ...
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bioRxiv - Biophysics 2024Quote: ... The amplified products were digested with BamHI and EcoRI (TaKaRa Bio, Shiga, Japan). The fused FP genes ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated with TALON® Metal Affinity Resin (Clontech, #635502) at 4°C for 30 minutes and the resin was washed with six column volumes of wash buffer A (50 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... and the supernatant was incubated with TALON IMAC resin (Clontech) and 20 mM imidazole for 12 h at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were then isolated through gel electrophoresis and purified using the Nucleospin gel purification kit (Takara Bio,740609.250). Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent Escherichia coli (Takara Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP libraries were prepared with the ThruPLEX® DNA-Seq Kit (Takara) and sequenced on the Illumina NovaSeq6000 SP-100 platform ...
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bioRxiv - Cancer Biology 2024Quote: KTC1 and PE121410 cells with RBM10 loss-of- function mutations were used to generate dox-inducible RBM10 expressing cells (KTC-RBM10 and PE-RBM10) by transducing the pLVX-Tet-On Advanced vector system (Clontech) with RBM10 cDNA (NM_005676 ...
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bioRxiv - Immunology 2024Quote: ... Samples were then processed for the preparation of libraries using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... the corresponding miR-8 target sequence was inserted downstream of GFP in pAcGFP (Clontech) using the XhoI/NotI restriction enzyme site ...