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Citations for Takara Bio :
1601 - 1650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized using 2 μg RNA and PrimeScript RT Master Mix (Takara Bio). qRT-PCR was performed using the first-strand cDNAs diluted 5-fold in water and KAPA SYBR FAST qPCR Master Mix (2x ...
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bioRxiv - Genomics 2024Quote: ... The protease and cleaved C-terminal tags were removed using Ni-NTA resin (Takara, 635662), and the proteins were further purified by size-exclusion chromatography on a HiLoad 26/600 Superdex 200 pg (Cytiva) ...
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bioRxiv - Genetics 2024Quote: ... and 5 μg of the pseudotyping pVSV-G plasmid were co-transfected into HEK293T cells in T75 flasks using CalPhos™ Mammalian Transfection Kit (631312, Takara Bio). The culture medium was replaced 12 hours after transfection with fresh media ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and performed PCR using Tks Gflex DNA Polymerase (Takara) according to the manufacturing protocol.
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bioRxiv - Genetics 2024Quote: ... The extracted RNA was quantified by Nanodrop and 500 ng of it was converted into cDNA using a PrimeScript™ RT reagent Kit (Takara). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Genetics 2024Quote: ... Extracted RNA was then reversely transcribed using the PrimeScript™ RT reagent Kit (Takara). RNA-seq libraries for expression analysis were constructed using KAPA RNA Hy-perPrep Kit KR1350 v1.16 according to the vendor’s protocol and paired-end 2 x 150 bp reads were sequenced using the Illumina HiSeq platform ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were transduced using lentivirus at an MOI between 5 and 10 on Retronectin-coated (Takara, T100B) plates overnight to express an M5 or SS1 CAR ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted using RNAiso plus (Takara, Japan). RNA (1 μg ...
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bioRxiv - Immunology 2024Quote: ... and converted to cDNA by using PrimeScript RT Master Mix (Takara), following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... coli cells (Takara Bio) and plated on LB agar plus carbenicillin ...
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bioRxiv - Immunology 2024Quote: ... and concentrated using Lenti-X Concentrator (#631232, Takara) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from 250 µl of EDTA-anticoagulated venous blood samples collected on-site using RNAiso Blood solution (Cat# 9112; Takara Bio, Japan) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a PrimeScript RT reagent Kit (Cat#RR047B, TaKaRa) and was used as a template to perform qPCR in TB green-based qPCR instrument (CFX-96 ...
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Assessment of the transcriptional regulation of EMT and MET through accessible transposable elementsbioRxiv - Genomics 2024Quote: ... Protein concentration was determined by the BCA Protein Assay Kit (Takara). Protein samples were then diluted with Laemmli Sample buffer ...
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bioRxiv - Immunology 2024Quote: cDNA was amplified from singly sorted B cells using SMART-Seq v4 (Takara Bio) at half reaction volumes ...
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bioRxiv - Synthetic Biology 2024Quote: ... and assembled into the vector using In-Fusion Snap Assembly Premix Kit (Takara Bio. Tech.). All plasmids were extracted using TIANprep Mini Plasmid Kit (TIANGEN Biotech.) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resin was washed twice by incubating it with 1 mL equilibration buffer for 10 minutes (Takara), centrifuging the resin (700xg for 5 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... Ribo-seq RNA libraries were prepared for sequencing using the SMARTer® smRNA-Seq Kit for Illumina (#635030) from Takara Bio USA ...
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bioRxiv - Synthetic Biology 2024Quote: ... the rRNA-depleted and fragmented total RNA fraction was converted to sequencing libraries using the SMARTer® smRNA-Seq Kit for Illumina (Catalog number #635030) from Takara Bio USA ...
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bioRxiv - Neuroscience 2024Quote: ... The α-syn140-YFP sequences were then subcloned into the pIRESpuro3 vector (Takara) using restriction cloning with EcoRV (5’ ...
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bioRxiv - Microbiology 2024Quote: ... Multiplex reverse transcription-PCR amplification of all 8 influenza virus genome segments was performed on RNA samples using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara 6210) and primers Uni12/Inf1 (5’-GGGGGGAGCAAAAGCAGG-3’) ...
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bioRxiv - Microbiology 2024Quote: cDNA synthesis and amplification were performed using SMARTSeq V4 Ultra Low Input RNA kit (Takara Bio). Then dual-indexing library preparation was created in equimolar conditions following the SMARTSeq Library Prep kit (Takara Bio ...
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bioRxiv - Genomics 2024Quote: ... Alu6B and Aluh1.1 were synthesized as gene blocks (IDT) and cloned into BamHI-AatII sites in the pYa5-neo vector using Infusion cloning kit (Takara) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Microbiology 2024Quote: ... The fixed section was stained with SYBR Green I (Takara-Bio, Inc., Japan) for 30 min ...
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... coli DH5α competent cells (Takara, Japan), which were subsequently screened for the presence of recombinants via PCR.
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bioRxiv - Cell Biology 2024Quote: RNAiso Plus (TAKARA) is used for RNA isolation ...
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bioRxiv - Microbiology 2024Quote: High-throughput qPCR was performed by an outside firm (Resistomap, Helsinki, Finland) using the qPCR SmartChip Real-Time PCR cycler (Takara; Kusatsu, Japonia). The qPCR cycling conditions and initial data processing were carried out as previously described by Wang et al ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP was PCR-amplified from pEGFP-N1 (Clontech) and used for Gibson cloning ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant with the protein was then separated by ultracentrifugation (60,000 rpm, 45 min, 4°C) and incubated overnight with Talon metal affinity resin (Takara Bio). The protein was affinity purified from the solubilized fraction in a gravity column (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... and cDNA was synthesized using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: Purified proteins or the artificial motor-cargo complex were diluted using 1× PBS (Takara Bio, T900) and loaded into custom-made microneedles prepared with the P1000IVF micropipette puller (Sutter Instrument) ...
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bioRxiv - Biophysics 2024Quote: ... Soluble protein in the supernatant was purified by Talon metal affinity resin (Takara Bio) and eluted into the elution buffer (50 mM sodium phosphate buffer (pH 7.2 ...
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bioRxiv - Biophysics 2024Quote: ... and pH 7.2) supplemented with the ProteoGuard EDTA-free protease inhibitor cocktail (Clontech, 635673). The suspended solution was put on ice and homogenized using a sonicator (Qsonica ...
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bioRxiv - Biophysics 2024Quote: ... DNA anchor (3’-alkyne-modified) was purchased from Fasmac (Kanagawa, Japan). RNase H from E. coli (Product No. 2150A) was purchased from Takara Bio Inc ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from the isolated RNA using the PrimeScript RT reagent (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... using Xfect Transfection Reagent (Takara, 631318) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Programed cell death was induced by incubating cells in complete DMEM with 1 mM B/B homodimerizer (Clontech) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were prepped using the NucleoBond Xtra Midi Plus kit (Takara Bio).
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bioRxiv - Cancer Biology 2024Quote: ... T cells were then plated on retronectin coated plates (Takara, T100A), stimulated with Human CD3/CD28 T Cell Activator (Stemcell Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... These 3xFLAG-CENP-T mutants were then cloned into the plasmid containing approximately 2 kb homology arm around the start codon of CENP-T with the neomycin resistance gene cassette and hisD gene cassette by In-Fusion® HD (TAKARA) (pBS_CENP-T N-KI_3xFLAG-CENP-TWT ...
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bioRxiv - Cell Biology 2024Quote: The transfected cells were selected in the medium containing appropriate selection drugs (0.5 µg/mL puromycin (TAKARA) for the selection of PuroR expression ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellartis human iPSC line 12 (ChiPSC12, #Y00280) was purchased from Takara Bio ...
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bioRxiv - Biophysics 2024Quote: ... the supernatant was incubated with 1 ml 50% v/v Talon resin (Clontech) and 5 mM imidazole pH 8.0 per initial litre of cell culture for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg/ml Doxycycline (Dox) (Clontech) was added to the cultured cells’ medium 24 hours before injection ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-7 and ErbB2-overexpressing MCF-7 cells were routinely tested using the CycleavePCR Mycoplasma Detection Kit (Takara bio, CY-232). None of the cell lines were contaminated with mycoplasma.
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bioRxiv - Bioengineering 2024Quote: ... The 500bp PCR fragments were purified by DNA electrophoresis and gel extraction(TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0) ...
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bioRxiv - Biophysics 2024Quote: ... and 5% fetal bovine serum (tetracycline-free FBS, Takara Bio) along with 10 U ml−1 penicillin and 10 μg ml−1 streptomycin (Pen-Strep ...
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bioRxiv - Biophysics 2024Quote: ... to reduce the detergent concentration and incubated for 3 h with 10 mL Talon metal affinity resin (Takara Biotech) pre-equilibrated with buffer S supplemented with 0.1% β-DDM and 0.02% CHS ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech) per manufacturer’s recommendations ...