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Citations for Takara Bio :
1701 - 1750 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Plasmids were constructed by standard molecular biology methods such as polymerase chain reaction (PCR) and In-fusion cloning (Clontech). Mutations for specific amino acids were generated by overlap-extension PCR ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... CDC-USA assay targeting gene N (IDT # 10006713) and One Step PrimeScript™ III RT-PCR Kit (TaKaRa #RR600A). Serial dilutions of reference material were prepared ranging from 1 to ~10M genome equivalents per reaction ...
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bioRxiv - Evolutionary Biology 2020Quote: The water-in-oil droplets after the incubation step were diluted 10000-fold with 1 mM EDTA (pH 8.0) and subjected to RT-qPCR (PrimeScript One Step RT-PCR Kit (TaKaRa)) with primer 1 and 2 after heating at 95 °C for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... They are routinely tested for contamination of mycoplasma by using PCR Micoplasma Test Kit (Takara Bio Inc., Shiga, Japan) and confirmed to be negative before performing experiments.
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bioRxiv - Evolutionary Biology 2022Quote: ... The measurement was performed after diluting the droplets 100-fold with 1 mM EDTA (pH 8.0) and using One Step TB Green PrimeScript PLUS RT-PCR Kit (Takara).
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RTEL-1 and DNA polymerase theta promote subtelomeric DNA synthesis and telomere fusion in C. elegansbioRxiv - Genetics 2022Quote: PCR for the ITS-DNA synthesis assay was carried out using ExTaq polymerase (Takara Bio cat# RR001A, Kasatsu, Japan). 35 cycles were executed with 64°C annealing temperature and 1 minute extension time ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNA was reverse transcribed and applied for PCR/qPCR analysis with PrimeScript™ reverse transcriptase (2680B, Takara, Japan). As an internal control for normalization ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of the reaction mixture was prepared with 5 μl of SapphireAmp Fast PCR Master Mix (Takara #RR350B), 1 μl of each of forward and reverse primers (final concentration 0.2 μM) ...
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bioRxiv - Microbiology 2022Quote: Amplified spike sequence was first gel-purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then further purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... Viral load was measured by RT-qPCR using One-Step SYBR® Primescript(tm) RT-PCR kit II (Takara). CT values from serum samples were used to calculate serum viral load according to regression equation built by a set of standard viral RNA extracted from dilutions of known titre virus preparation ...
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bioRxiv - Plant Biology 2022Quote: ... aril and kernel tissues were pooled equally and cDNA was synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech). Size fractionation and selection (1-2 ...
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bioRxiv - Neuroscience 2022Quote: ... Gαi2 and Trpc2 cDNAs were amplified by PCR from female rat VNOs (PrimeSTAR® GXL DNA Polymerase Takara, Japan). Vom1r68 was synthesized by BGI (China) ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was used as template for PCR using TaKaRa Ex Taq or PrimeSTAR GXL DNA polymerase (Takara Bio), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative RT-PCR was used to determine gene expression using the SYBR Green Realtime Master Mix (Takara, DaLian, China). Table 1 contains a list of all primers used in this study ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplification from genomic and plasmid DNA templates was performed using PrimeStar Max DNA polymerase (Takara Bio, Kusatsu, Japan) or GoTaq Master Mix (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... T-DNA insertions were then analysed using specific primers (Table S1) in PCR reactions with Emerald Master Mix (Takara). PCR conditions were as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR analysis was performed with Light Cycler-480®II (Loche) using TB-Green (Takara, RR420) following the supplier’s instructions.
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bioRxiv - Microbiology 2023Quote: ... and fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara). Finally ...
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bioRxiv - Microbiology 2023Quote: ... Real time PCR was carried out in 20 µl reaction mixtures containing 1x TB Green Premix Ex Taq (Takara), 1x ROX reference dye (Takara) ...
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bioRxiv - Cancer Biology 2023Quote: ... real-time PCR was performed using TB Green® Premix Ex Taq™ (Tli RNaseH Plus) (Takara Bio RR420A). The signals were measured in the QuantStudio 3 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Genetics 2023Quote: ... The PCR fragments were purified and inserted into the vector pigAct88F-GFP [58] through recombinational cloning (In-Fusion, Takara). Primers used for the PCR reactions (sequences for recombination are underlined):
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bioRxiv - Microbiology 2023Quote: ... High-throughput qPCR (HT-qPCR) was performed by the Smart Chip Real-time PCR system (Takara Bio USA, Inc.). A non-template negative control was set for each primer set ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The genotyping was performed using PCR and 1.5% agarose gel electrophoresis using EX Taq Hot Start Version (TaKaRa Bio) according to the manufacturer’s protocol and two designed primers ...
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bioRxiv - Cancer Biology 2023Quote: ... RRM2 and RRM2B fragments were generated using HepG2 endogenous DNA and PCR amplified using Clone Amp HiFi (Takara #639298). Fragments were purified on a DNA gel and extracted (Qiagen #28706) ...
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bioRxiv - Developmental Biology 2023Quote: ... TRE3Gs and Tet-ON 3G were amplified by PCR from AAVpro Tet-One Luc Control Vector (Clontech, Cat# 634311), Kaede from in-house recombineering cassette (Zheng et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The 16S rRNA V4 region was PCR-amplified with buffer and polymerase PrimeSTAR GXL DNA Polymerase (Takara, Cat: R050B). The cycling conditions for the PCR are as follows:_98 °C for 3 min ...
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bioRxiv - Cancer Biology 2022Quote: ... using 5′-GGGTTAGGGATAGGCTTAC-CACCGGTTTACTTGTACAGCTCGTCCATGC -3′ and 5′-CTTGTACAAAGTGGTTACCGGAGGATC-CGGTGGTGTGAGCAAGGGCGAGGAGCTG -3′ primers for PCR and In-Fusion Cloning (Takara Bio). The pStrep-ANKLE1 plasmid was obtained by cloning annealed oligonucleotides containing Twin-Strep-tag (5′ CCGGTCACCATGGCGTGGAGCCACCCGCAGTT-CGAGAAAGGTGGAGGTTCCGGAGGTGGATCGG-GAGGTTCGGCGTGGAGCCACCCGC-AGTTCGAAAAAGC 3′ and 5′ GGCCGCTTTTTCGAACTGC-GGGTGGCTCCACGCCGAACCTCCCGAT-CCACCTCCGGAACCTCCACCTTTCTCGAA-CTGCGGGTGGCTCCACGCCATGGTGA 3′ ...
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bioRxiv - Developmental Biology 2023Quote: ... Start codon mutant constructs were generated by mutation PCR using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Kusatsu, Japan) by replacing AUG with AAG at Met1 ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry was introduced into the pcDNA™3.1-BspEI-LRP10 plasmid via PCR amplification from the pmCherryN1 plasmid (Clontech) via the addition of BspEI restriction sites in the primer sequences ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed using 500 ng of RNA in 10 µl volume of Solution with PrimeScript RT (TAKARA) and random hexamer primers ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplified and subcloned into a pCG-strep expression construct using the In-Fusion® HD Cloning Kit (Takara) according to the manufacture’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was collected using a CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (TaKaRa, Cat# 3732) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Full-length cDNA was synthesized with 1.0 μg RNA from Diphasiastrum complanatum using the SMARTer PCR cDNA Synthesis kit (Clontech). The first-strand cDNA was amplified with PrimeSTAR GL DNA polymerase (Clontech ...
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bioRxiv - Neuroscience 2023Quote: ... and the target bands at 14,438 bp were cut and purified with NucleoSpin® Gel and PCR Clean-Up kit (740609.250, Takara). The target amplicons were re-concentrated to > 300 ng/ µL with GlycoBlue™ Coprecipitant (AM9515 ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR fragments of AGB1.x were ligated into the pGBKT7 DNA BD vectors (Takara Bio USA, CA, USA) and AGGs into the pGADT7 AD vector (Takara Bio ...
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bioRxiv - Plant Biology 2024Quote: ... the AtTPT gene was first amplified by PCR and then introduced into the pMD19-T vector (TaKaRa, Dalian, China) to construct pMD19-T-AtTPT ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was collected using a CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (TaKaRa, Cat# 3732). GAPDH and vRNA levels were measured with a RT-qPCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) ...
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bioRxiv - Cell Biology 2023Quote: A cDNA fragment encoding human FAM53C was isolated by amplifying with nested PCR from human cDNA library plasmid (Takara). The oligonucleotide primer sequences used for the 1st PCR are 5′-CAAAGTGTGCAAGTCAAATCCTGG-3′ (5′ upstream ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using a Thermal Cycler Dice Real Time System II (Takara Bio Inc., Otsu, Japan) and SYBR Premix Ex Taq II (Takara ...
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bioRxiv - Cancer Biology 2023Quote: Real-time PCR was carried out using a TB Green Premix Ex Taq (Tli RNaseH Plus) kit (Takara, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... A relative transcript expression level for genes was measured by quantitative real-time PCR using a SYBR Green (Takara) based method ...
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bioRxiv - Cancer Biology 2024Quote: ... The 13 kb band was purified with gel purification kit (Takara Nucleospin® Gel and PCR Clean-up Midi). Eighty-two base pair single stranded oligos were designed according to the following structure:
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bioRxiv - Immunology 2024Quote: ... and PVI.V-12 were amplified via PCR and cloned into the LALA and LALAPG IgG1 vectors using InFusion cloning (Takara).
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCR was performed using a Thermal Cycler Dice Real-Time System II TP900 (Takara Bio, Shiga, Japan), and the relative copy number of various gene transcripts per beta-actin transcript was determined using calibration standards for each tested molecule ...
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bioRxiv - Microbiology 2024Quote: Quantitative PCR (qPCR) was conducted using a Thermal Cycler Dice Real Time System III (Takara Bio Inc., Shiga, Japan). For each 25 μL reaction ...
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bioRxiv - Bioengineering 2024Quote: Primers oAS344 and oAS345 were then both used to amplify the cDNA using CloneAmp PCR Master Mix (Takara 639298) according to manufacturing protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... 5xmyc-RPGRIP1L cDNA was amplified from the plasmid pCS2-5xmyc-RPGRIP1L (23) using CloneAmp HiFi PCR Premix (Takara # 639298) using primers ...
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bioRxiv - Molecular Biology 2024Quote: To generate APRF1pro:APRF1-mVENUS and TOPP4pro:TOPP4-3xFLAG the genomic region of both genes including a region of around 1.5 kb upstream their transcription start sites were amplified by PCR and cloned by the InFusion cloning (Takara) into the pCAMBIA1300 in frame with the coding sequences of mVENUS or the 3xFLAG peptide ...
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bioRxiv - Plant Biology 2020Quote: ... and Real-time qRT-PCR was performed using a Thermal Cycler Dice® Real Time System III T950 (Takara, Japan) with gene-specific PCR primers (Supplemental Table S1) ...
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bioRxiv - Plant Biology 2020Quote: The pPIGS:GFP-PIGS construct was created by overlap PCR with PrimeSTAR® GXL DNA Polymerase (TaKaRa Bio Inc.; Catalog # R050A) and DNA templates and primers listed in Additional Table 1 and cloned into pH7WG plasmid linearized with SalI-HF (NEB ...