Labshake search
Citations for Takara Bio :
1851 - 1900 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Seven Ns were added into the primers as the barcode for barcoded-subamplicon sequencing during the first round PCR (PrimeSTAR Max DNA Polymerase, Takara Bio ...
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RNA quantification was performed by RT-qPCR targeting the S gene of SARS-CoV-2 using One Step PrimeScript RT-PCR Kit (Takara) with the following SARS-CoV-2 specific primers and probes ...
-
bioRxiv - Genetics 2022Quote: ... Reverse transcription was performed using the oligo(dT) primer and avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). RT-qPCR was performed using the Kapa SYBR FAST qPCR kit (Kapa Biosystems ...
-
bioRxiv - Microbiology 2022Quote: The following plasmids were generated by subcloning polymerase chain-reaction (PCR) generated amplicons from the indicated oligonucleotides from Table 1 into pEFtak or pLEX vector using In-fusion recombinase (Takara) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... the GST gene from the pEGKG plasmid (29) was amplified by PCR and inserted immediately upstream of GFPS65T using the In-Fusion HD cloning kit (Takara).
-
bioRxiv - Microbiology 2019Quote: ... All ∼5 kb products were amplified from bat genomic DNA by inverse PCR employing the PrimeSTAR MAX DNA polymerase (Takara), purified by gel extraction (Axygen) ...
-
bioRxiv - Pathology 2020Quote: ... 3’-RACE-PCR was performed as per the instructions of SMARTer™ RACE-cDNA Amplification Kit User Manual (Clontech, 2012) using primers given in Table 1 ...
-
bioRxiv - Genomics 2019Quote: ... The Human EF1a Promoter/Tet-On 3G fragment as well as the Neo(R) cDNA were PCR amplified from the pEF1α-Tet3G (Cat. # 631336, Clontech) using the following primer pairs
-
bioRxiv - Cell Biology 2019Quote: ... We then generated by PCR a Linker+ GFP/mCherry which we inserted into the dyn2 vector with the In-Fusion HD kit (Clontech). Dyn2-GFP-ΔCter and Dyn2-GFP-ΔPRD were generated introducing a stop codon at positions 807 (after motif A ...
-
bioRxiv - Genomics 2019Quote: ... PCR amplifications were performed on 1 μl of 1:20 dilution of the cDNA template in a final volume of 50 μl using the EmeraldAmp PCR Master Mix (Clontech). Appropriate annealing temperatures and cycle conditions were adjusted to individual primer pairs (see supplemental methods).
-
bioRxiv - Physiology 2019Quote: ... a tetracycline-regulated Kcnn4 expression construct was generated by subcloning PCR-amplified cDNA encoding the open reading frame of murine Kcnn4 (gene ID16534)) into the pTRE-Tight expression vector (Clontech). The construct was verified by sequencing ...
-
bioRxiv - Cell Biology 2019Quote: ... The coding sequences of these small GTPase proteins were amplified with polymerase chain reaction (PCR) using KOD-Plus-Neo DNA polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as template cDNA ...
-
bioRxiv - Biochemistry 2020Quote: ... Each PCR product was subcloned into the NheI and XhoI sites of a cytomegalovirus promoter-driven pIRES2-AcGFP1 vector (Clontech Laboratories Inc. ...
-
bioRxiv - Genetics 2019Quote: ... The gene fragments of off-target sites were amplified with primers specific to each locus by 35 cycles of PCR with TaKaRa LA Taq (TaKaRa). The PCR products were purified and subjected to the process of denatured and annealed by using a thermocycler ...
-
bioRxiv - Molecular Biology 2019Quote: Coding sequences of rat Rbpms isoforms were PCR amplified from differentiated PAC1 cell cDNA and cloned into XhoI/EcoRI sites of the pEGFP-C1 vector (Clontech) and into EcoRI/XhoI sites of the pCI-neo-3x-FLAG vector (Rideau et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... ERT2-3HA fragment was then PCR amplified using 2Fw and 2Rv primers and inserted between BmtI and BamHI sites of pmCherry-C1 (Clontech). The TBP coding sequence was PCR amplified from human cDNA and was cloned into SalI site of this plasmid containing ERT2-3HA ...
-
bioRxiv - Cell Biology 2019Quote: ... followed by 30 cycles of PCR for amplification using the Ex Taq hot start version (Takara Bio Inc., Otsu, Japan) with 50 pM of primer sets for mouse mRNA of β-actin ...
-
bioRxiv - Immunology 2019Quote: ... We used 0.2 μg of total RNA for reverse-transcription reactions using Advantage RT-for-PCR kit (TakaRa Bio USA). cDNA was used for RT-PCR ...
-
bioRxiv - Bioengineering 2020Quote: ... Ligation products with the correct size were purified by DNA agarose gel extraction using NucleoSpin Gel and PCR Clean-Up Kit (Takara, 740609.250 ...
-
bioRxiv - Genomics 2020Quote: ... and quantitative real time PCR was performed following manufacturer’s instructions given in SYBR1 Premix ExTaq (TliRNAse H Plus) (Clontech,USA). The PCR amplification was carried out in Rotor-Gene-Q Real-Time PCR System (Qiagen ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Homology arms were assembled into digested (SacI, XbaI) or PCR-amplified pLO3 backbone via Gibson Assembly (HiFi, NEB or In-fusion, Takara). C ...
-
bioRxiv - Cell Biology 2019Quote: ... TRPV4(ΔPR)–myc and TRPV4(ΔAR1-3)–myc were constructed using PCR with template plasmids from Open Biosystems subcloned into pCMV-myc vector (Clontech) at the SalI/XhoI sites ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: 5’ and 3’ cDNA ends were subsequently amplified by RACE PCR using the SMARTer RACE cDNA Amplification Kit and Advantage HF2 DNA polymerase (Clontech) with gene-specific primers derived from the initial RT-PCR product (Supplemental Information ...
-
bioRxiv - Cell Biology 2020Quote: ... Mouse E-Syt3 cDNA was amplified by PCR from a cDNA library constructed with poly(A) RNA from 3T3-L1 adipocytes (Mate & Plate TM Library System, Clontech) and cloned into the PT-Adv vector using 5’and 3’ E-Syt3 primers (Table 1) ...
-
bioRxiv - Genetics 2021Quote: ... This mutated fragment was replaced by the original homologous sequence of the pJet-ebony_mut-mCherry vector by fusing the mutated fragment and the PCR product amplified from the pJet-ebony-mCherry vector using the In-Fusion HD Cloning Kit (TaKaRa) with the following primers ...
-
bioRxiv - Genetics 2021Quote: ... Reverse transcription was then performed using the oligo (dT) primer and Avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). We conducted RT-PCR using KOD One (TOYOBO ...
-
bioRxiv - Cell Biology 2020Quote: ... of human ACLY was PCR amplified from a HeLa cDNA pool and inserted into the EcoRI-digested pLVX-puro vector (Takara) using the In-Fusion cloning system (Takara) ...
-
bioRxiv - Developmental Biology 2019Quote: ... The cDNA was diluted 1:1 with nuclease free water and subjected to qRT-PCR using SyBr green mix (Takara) in real-time PCR machine (BioRad CFX96) ...
-
bioRxiv - Molecular Biology 2021Quote: ... cDNAs were PCR-re-amplified and finally subcloned into Hind III site of the pEYFP-C1 or pECFP-C1 vectors (Clontech).
-
bioRxiv - Cancer Biology 2019Quote: ... 20-50ng of the first PCR amplicon in a total PCR reaction volume of 25ul was amplified in a 12 cycle PCR using primer set 2 and PrimeSTAR GXL DNA Polymerase (Takara) (Supplemental Table 1b) ...
-
bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was performed using SYBR Premix Ex TaqII (Tli RNaseH Plus) and the Thermal Cycler Dice Real Time System (TaKaRa). The results were analyzed based on standard curves from a serial dilution of cDNA ...
-
bioRxiv - Molecular Biology 2021Quote: A DNA fragment encoding the full-length LARP1 gene (NM_033551) was PCR- amplified from HEK293T genomic DNA and inserted into the pColdI vector (TaKaRa, 3361) at the NdeI and HindIII sites ...
-
bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and detected by RT-qPCR assays with a One-Step PrimeScript RT-PCR kit (Takara, Japan) using SARS-CoV-2-specific primers on an Applied Biosystems 7500 Real-time PCR System.
-
bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A) using LightCyclerW 480 (Roche ...
-
bioRxiv - Immunology 2021Quote: ... Initial PCR amplification and the subsequent barcoding PCR reaction of the 3BNC117 VH fragments or the Off Target sites was performed using the proofreading PrimeStarMAX Polymerase (Takara) for 35 cycles and 8 cycles ...
-
bioRxiv - Developmental Biology 2019Quote: ... 10 ng total RNA was reverse transcribed and full-length cDNA was specifically amplified by 8 PCR cycles using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) (31) ...
-
bioRxiv - Biochemistry 2019Quote: ... A human Podocin-N fragment cDNA with 3xFLAG was amplified by PCR using a human kidney cDNA in Human MTC Panel I (TaKaRa) as a template ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Forward (control) and reverse (target) RNA probes were synthesized from plasmids amplified via PCR (Advantage HD Polymerase Kit, Takara Bio) using T7 or SP6 RNA Polymerases (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... RNA with an OD value (A260/A280) of 1.8–2.0 was reversed transcribed to cDNA by using a PrimeScriptTM RT-PCR kit (Takara, Kusatsu, Japan). cDNA was amplified by Takara Taq™ (DR001AM ...
-
bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified using a One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (Takara Bio) on a StepOnePlus real-time PCR system (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... an additional set of genes encoding pTF.CREG1 with N-terminal truncations (Δ26, Δ31, Δ39, Δ43) was generated by PCR using PrimeStar GXL DNA Polymerase (Takara Bio), primer sets JT33/JT32 ...
-
bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR and inserted into NheI and AgeI sites of the mCherry-C1 and mCerulean3-C1 vectors (Clontech). YFP-PH(Akt ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The relative expression of genes was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) analysis using SYBR Green Supermix (TaKaRa); β-actin served as a housekeeping gene ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Real-time reverse transcription quantitative PCR (qPCR) was performed in duplicate using 1 μL of cDNA from each sample and SYBR Premix Ex Taq (Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... The fragment length of PCR products was detected by 1.2% agarose gel electrophoresis using 5 μl of 100 bp DNA Ladder (TaKaRa, Japan) and gel stain (TransGen Biotech ...
-
bioRxiv - Genetics 2021Quote: ... The composition of the reaction mixture for the PCR was as follows: 10 μl Premix Taq (Ex Taq Version 2.0 plus dye, TaKaRa, Japan), 0.3 μl each of forward and reverse primer (10μM) ...
-
bioRxiv - Biochemistry 2021Quote: ... hCLPTM1L-FLAG-6His was amplified by PCR and cloned into pPB-tetON-BSD-tightFF plasmid by In-fusion cloning (Clontech). To construct pPB-tetON-Tmem16k-FLAG-6His-BSD-tightFF plasmid ...
-
bioRxiv - Molecular Biology 2021Quote: ... the region from the start of eBFP1.2 until the end of iGFP was PCR-amplified from the modified pDRGFP and ligated into XbaI/MluI digested pLVX-IRES-Hyg (Clontech; this procedure removes the IRES-Hygro sequence) ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA synthesis from the total RNA was carried out using the PrimeScriptTM High Fidelity RT-PCR Kit (TaKaRa, Shiga, Japan) using an oligo dT primer ...
-
bioRxiv - Cell Biology 2021Quote: ... Borr open reading frame corresponding to amino acids 113-221 was first amplified with a stop codon from LD36125 by PCR using PrimeStar (Takara) and cloned between AscI and NotI sites of pENTR (ThermoFisher ...