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Citations for Takara Bio :
1501 - 1550 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... DNA in the supernatant was purified using the NucleoSpin Gel & PCR kit (Takara Bio, Kusatsu, Shiga, Japan). Sequencing libraries were prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche ...
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bioRxiv - Immunology 2023Quote: ... and quantified by real-time PCR with TB Green Premix Ex Taq (TaKaRa Biomedical Technology Co., Ltd.) and ABI 7500 (Applied Biosystems Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... The viral titer was measured using a Lenti-X qRT‒PCR Titration Kit (#631235, TaKaRa, Shiga, Japan).
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bioRxiv - Bioengineering 2024Quote: ... Viral genome number was quantified by real-time PCR with AAVpro Titration Kit Ver.2 (Takara, 6233) according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2024Quote: ... The linear PCR product was then re-circularized with In-Fusion Cloning Master Mix (Takara Bio, 638948) according to manufacturer’s protocol.
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bioRxiv - Genomics 2024Quote: ... except that an alternative oligo-dT primer from the SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.), which also included a random 10mer sequence as a unique molecule identifier (UMI ...
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bioRxiv - Genomics 2024Quote: ... The PCR reaction mix consisted of 1.25 PrimeSTAR GXL DNA Polymerase (Takara Bio USA, San Jose, CA), 1× Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... The promoter regions of target genes were amplified and quantified by qRT-PCR using SYBR Green (Takara, Japan) on an ABI 7500 Fast system ...
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bioRxiv - Cancer Biology 2021Quote: Full-length cDNA was synthesized from total RNA (1 μg) by SMARTer®□ PCR cDNA Synthesis kit (Clontech) according to the manufacture’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... SLD3 and ESA1 ORF were PCR-amplified with Taq polymerase (MightyAmp ver. 2, Takara Bio Co., Otsu, Japan) and cloned into the BamHI sites of the Y2H DB vector pST1667 and the Y2H AD vector pST548 (Tanaka ...
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bioRxiv - Cell Biology 2020Quote: Plasmids were constructed using standard molecular biology methods including polymerase chain reaction (PCR) and In-fusion cloning (Clontech). Mutations for specific residues were introduced by overlap-extension PCR ...
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bioRxiv - Cell Biology 2020Quote: ... fused with the SYFP2 coding region using recombinant PCR and cloned into pMSCV-Thy-IRES 1.1 vector (Clontech) using EcoRI/XhoI and ClaI restriction sites.
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bioRxiv - Cell Biology 2020Quote: ... Real-time PCR was performed using the Roche LightCycler 480 and the TB Green Premix Ex Taq (TaKaRa). PP2AA3 (AT1G13320 ...
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bioRxiv - Genetics 2021Quote: ... Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus) (Takara bio RR820W) and a Quantstudio6 Flex Real Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was amplified using PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio, Shiga, Japan) under the following reaction conditions ...
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bioRxiv - Molecular Biology 2022Quote: The RNA samples extracted from the fruit flesh were reverse transcribed using the PrimeScript RT-PCR Kit (TaKaRa) and the oligo(dt)20 primer ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative Real Time PCR reactions were performed with the TB Green™ Premix Ex Taq™ II (TAKARA) as recommended by the supplier ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNAs used for RT-PCR were synthesized using PrimeScript First-Strand cDNA Synthesis Kits (Takara, Osaka, Japan). RT-PCR cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... and one μg RNA was converted to cDNA by using the PrimeScript™ RT-PCR Kit (Takara, Japan) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR product was gel purified and ligated into a pET-28 vector using In-Fusion® (Takara) cloning.
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bioRxiv - Cell Biology 2019Quote: The coding sequence of AcGFP1 was amplified by PCR with the AcGFP_F and AcGFP_R primers and subcloned into the AgeI/NotI sites of pDsRed2-Mito (Clontech). The coding sequence of Venus (cDNA was obtained from Dr ...
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bioRxiv - Immunology 2019Quote: ... The mRNA quantifications of target genes were performed by real-time PCR using the SYBR GREEN MIX (Takara). Data were normalized to the expression of the housekeeping gene β-actin ...
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bioRxiv - Developmental Biology 2019Quote: ... The CDS of the EGFP and mCherry fluorescent tags were obtained by PCR from the pEGFP-C1 (Clontech) and pRSET-B-mCherry (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR analysis was performed using the SYBR Premix Ex TaqTM II kit (Takara, Dalian, China). HMGB1 primers were designed using Primier4.1 software and GADPH was used as a reference gene ...
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bioRxiv - Genomics 2019Quote: ... Long range PCR was performed using Advantage 2 Polymerase following the manufacturer’s protocol (Clontech Laboratories, Mountatin View CA).
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bioRxiv - Molecular Biology 2019Quote: ... Mixed cDNAs were used as a template for the subsequent PCR using PrimeSTAR Max DNA Polymerase (TaKaRa #R045A). The following PCR conditions were used ...
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bioRxiv - Evolutionary Biology 2019Quote: ... All PCR reactions were performed in 24 μl volumes with 13 μl ExTaq HS polymerase (Takara Bio Inc.), 1 μL each forward and reverse primers ...
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bioRxiv - Physiology 2021Quote: Real-time qRT–PCR was performed on cDNA amplified by PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) using GoTaq qPCR (Promega ...
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bioRxiv - Genomics 2021Quote: ... primer pairs specific for each investigated enhancer were used for PCR amplification with the EpiTaq HS polymerase (TaKaRa). Finally ...
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bioRxiv - Systems Biology 2021Quote: ... Other plasmid components (see Supplementary Table 1) were synthesized or PCR amplified with PrimerSTAR MAX DNA polymerase (TAKARA) and checked by Sanger sequencing ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT-PCR Kit (Takara). Three technical replicates were measured for gene expression levels in each cDNA sample ...
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bioRxiv - Biochemistry 2021Quote: ... The resultant PCR product and the QtoG IDR gBlock were used in an In-Fusion cloning reaction (Takara) to generate pENTR-ARF19 QtoG IDR ...
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bioRxiv - Microbiology 2020Quote: ... The qRT-PCR was performed using the TB Green Premix Ex Taq II (Takara Bio Inc., Shiga, Japan) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA of Human Phosphodiesterase type 10 (hPDE10) was amplified by conventional nested PCR using human brain cDNA (Clontech). cDNAs template encoding wild-type and D674A mutant form of hPDE10 catalytic domain (CD ...
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bioRxiv - Microbiology 2021Quote: ... Cells were originally obtained from ATCC and routinely tested negative for mycoplasma contamination (PCR Mycoplasma Detection Set, Takara).
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bioRxiv - Microbiology 2021Quote: ... The cassette was PCR-amplified from these plasmids utilizing TaKaRa Ex Taq™ DNA Polymerase (Clontech Takara Bio) and used for A ...
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bioRxiv - Microbiology 2021Quote: ... The cassette was PCR-amplified from these plasmids utilizing TaKaRa Ex Taq™ DNA Polymerase (Clontech Takara Bio) and used for A ...
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bioRxiv - Molecular Biology 2020Quote: ... The mAID-ORC2gr was then PCR and assembled into BglII/XhoI digested pMSCV-hygro retroviral vector (TaKaRa #634401). All cloning was done using In-Fusion cloning system (TaKaRa) ...
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bioRxiv - Microbiology 2020Quote: ... converted to single stranded cDNA and amplified using the SMARTer PCR cDNA Synthesis Kit (Takara Bio, Kusatsu, Japan), and IsoSeq libraries were constructed with equimolar cDNA fractions (0.5X and 1X ...
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bioRxiv - Cell Biology 2021Quote: ... total RNA was extracted using TRIzol reagent (Fermentas) and reverse transcribed using the mRNA Selective PCR Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA was quantified (by Nanodrop) and retrotranscribed to cDNA (PrimeScript™ RT-PCR Kit - Takara Bio Cat. # RR014A). Real time PCR was performed with KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR products were cloned into the HaloTag vector based on PB533 using an in-fusion system (TaKaRa). The expression vectors of H2B-Halo and Halo-PCNA were constructed by inserting their coding sequence (Kanda et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara). Values of gene expression were normalized to GAPDH expression using the ΔΔCT method.
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex Taq™ kit (TaKaRa) on a Roche LightCycler 480 real-time PCR machine ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology, Beijing, China), 0.2 µL bovine serum albumin (BSA) ...
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bioRxiv - Bioengineering 2021Quote: ... The PCR amplified CLIPtag were then ligated with the digested vectors using In-Fusion HD Cloning kit (Clontech). The plasmids pSNAPf-Clathrin-15 and pSNAPf-LMNA were directly purchased from UCSF Nikon Imaging Center ...
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bioRxiv - Plant Biology 2021Quote: ... Synthesis of cDNA and quantitative PCR was conducted using the PrimeScript RT Master Mix (Takara Bio, Kusatsu, Japan) with Thermal Cycle Dice TP800 (Takara Bio) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 μg of RNA was reverse transcribed in a 20 μL volume with RT PCR master mix (TaKaRa) as per the manual instruction ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time PCR analysis was performed using the ABI7500 machine and SYBR Premix Ex Taq Mix (Takara #RR420A). LOC_Os06g48970 was used as an internal reference as described previously (Dreni et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The purified mixture of PCR products was cloned into pMD20-T vector using Mighty TA-cloning Kit (TaKaRa). The 119 clones for AAVS-tid GTC_70-107 (+ ...