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Citations for Takara Bio :
1601 - 1650 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: The full-length MpACL5 amplified from Tak-1 cDNA by PCR using TaKaRA EX Taq (Takara, Shiga, Japan) with the primers ...
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bioRxiv - Microbiology 2023Quote: ... GISAID ID: EPI ISL 408667) [30] were generated by PCR using PrimeSTAR GXL DNA polymerase (TAKARA, Cat# R050A). A linker fragment comprising hepatitis delta virus ribozyme ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Complete mtDNA was amplified in several overlapping fragments using the Long and Accurate (LA) PCR kit from TAKARA.
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bioRxiv - Synthetic Biology 2023Quote: ... Specific primers were designed for this purpose and PCR was done using the Advantage 2 Polymerase kit (Clontech). Complementary 60 bp-ends to each target sequence on all Mycoplasmas species genome used in this study ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Plasmids were assembled from linear fragments using In-Fusion HD (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: The 16S rRNA gene sequences were amplified by PCR using LA Taq polymerase (TaKaRa-Bio, Inc., Shiga, Japan) using the primers Uni530F and Uni907R65 ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA fragment encoding zebrafish Foxo1a was amplified by PCR and subcloned into pEGFP-C3 vectors (Takara Bio Inc.) to generate the expression plasmids ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Final plasmids were constructed using In-Fusion HD (Takara Bio ...
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bioRxiv - Genetics 2022Quote: ... The purified PCR products were assembled into EcoRI-HF/XhoI-digested pAct-Cas933 by In-Fusion cloning (Takara).
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bioRxiv - Plant Biology 2023Quote: ... and AtMYB62 genes were amplified by PCR using gene specific primers and cloned into pGADT7-Rec vector (Takara). The recombinant constructs were transformed into Y1H gold strain (Takara) ...
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bioRxiv - Immunology 2023Quote: ... linearized BF520 sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Physiology 2023Quote: ... Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus) (Takara bio RR820W) and a QuantStudio 6 Flex Real Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was cloned into SalI-PstI-digested pKG120 by In-Fusion HD Cloning Kit (Takara bio). The resulting plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... and the PCR products were cloned into the EcoRI/SalI multicloning sites of the pEGFP-N3 vector (Takara), designated as pEGFP-hEB3 ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR reactions were set up with FastStart DNA Master SYBR Green I master mix (Takara, Japan). For each analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR reactions were performed using SYBR Premix Ex Taq II with a thermal cycler (TP800, Takara Bio).
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bioRxiv - Biochemistry 2023Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed with TB Green Premix Ex Taq II (Takara). Primers were obtained from Takara Bio Inc ...
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bioRxiv - Neuroscience 2023Quote: ... First strand cDNA synthesis was performed on 200ng RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... was performed to determine the expression level of transcripts utilizing the SYBR Green PCR master mix kit (Takara). The unique region of transcripts was selected for designing qRT-PCR primers and mentioned in Table S3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCR) was conducted in duplicate samples utilizing TB Green Premix Ex Taq II (Takara, Cat# RR820S) and the Thermal Cycler Dice (Takara ...
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bioRxiv - Molecular Biology 2023Quote: A DNA fragment coding DDX43 or DDX6 was amplified by PCR and cloned into pCold-I vector (Takara) by In-fusion cloning kit (Takara).
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (TaKaRa Bio), nuclease-free PCR-grade water ...
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bioRxiv - Biochemistry 2023Quote: ... A gene region encoding TiCGSCy was amplified by PCR using PrimeSTAR Max DNA Polymerase (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was then performed on the cDNA using TB Green Premix Ex Taq II (RR820A, Takara), following the instructions manual.
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bioRxiv - Microbiology 2023Quote: ... Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Bioengineering 2024Quote: ... CD11b-specific amplicons were generated via 3-step nested PCR using PrimeSTAR GXL (Takara Bio, Kusatsu, Shiga, Japan) according to the manufacturer’s protocol with a 60°C annealing temperature ...
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bioRxiv - Neuroscience 2023Quote: ... All cell lines were tested for mycoplasma contamination regularly with PCR Mycoplasma Detection Set (TaKaRa, Cat. no. 6601) and maintained under passage 20 ...
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bioRxiv - Developmental Biology 2023Quote: ... we confirmed successful repair by extracting mRNA and performing one step RT-PCR (Takara Primescript High Fidelity, RR057B). Primer sequences and design are detailed in Supplementary Fig 1 ...
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bioRxiv - Genetics 2023Quote: ... electrophoresis was performed using 9 μL of the PCR products on a 2% agarose gel (Takara Bio, Japan) at 100 V ...
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bioRxiv - Cell Biology 2023Quote: ... and GFP-IDR-C (384-413 aa) were amplified via PCR and cloned into the pColdI vector (Clontech) at BamHI site ...
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bioRxiv - Molecular Biology 2024Quote: ... Real time PCR was set up using TB Green Premix Ex Taq II (Tli RNase H Plus) (TakaRa) in a Quantstudio5 machine ...
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bioRxiv - Cell Biology 2023Quote: ... The virus titer was quantified using the AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2024Quote: ... The amplified PCR fragments were recombined into the yeast bait two-hybrid vector pGBKT7 (Takara Bio, Kusatsu, Japan), previously digested with EcoRI and BamHI ...
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bioRxiv - Developmental Biology 2024Quote: ... and the PCR fragments from the mutant animals were ligated into a pMD19-T vector (TaKaRa, Osaka, Japan) in preparation for sequencing ...
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bioRxiv - Microbiology 2024Quote: ... linearized GPC sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... The purifications for PALS-C steps were done with NucleoSpin Gel and PCR Clean-Up kit (Takara, 740609). Final assembled products were delivered to Endura Electrocompetent Cells (Lucigen ...
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bioRxiv - Biophysics 2019Quote: A cDNA encoding human DBNL was amplified by PCR from Human Brain Whole QUICK-Clone™ cDNA (Clontech Laboratories) using the primers ...
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bioRxiv - Cell Biology 2020Quote: ... cells were single-cell sorted into 96-well low-bind PCR-plates [Eppendorf] containing 3 μl of lysis buffer (0.5 units/μl RNase inhibitor [Takara] ...
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bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed on three biological replicates using an ABI StepOne PCR detection system with SYBR Green (Takara). GmUbiquitin (SUBI-2.2 ...
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bioRxiv - Developmental Biology 2021Quote: ... ssDNA was purified using the Nucleospin Gel and PCR Clean-up kit (Machery-Nagel, distributed by Takara Bio USA) with buffer NTC used as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... Mutagenesis of pMU2-Camk2b was conducted by inverse PCR with Mighty Cloning Reagent Set (Blunt End) (Takara Bio, Japan) following to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... pAAV-SERT was digested with HindIII and AfeI and the PCR product was inserted by InFusion Cloning (Takara Clontech) to generate pAAV-SERT His-GCaMP6s ...
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bioRxiv - Neuroscience 2020Quote: ... at each end of the FP during PCR and was performed using In-Fusion HD enzyme premix (Clontech, USA). In all cases ...
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bioRxiv - Molecular Biology 2019Quote: Each polymerase chain reaction (PCR) was carried out in 50-μL volumes containing 2U Taq DNA polymerase (Takara Co.), about 60 ng template DNA ...
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bioRxiv - Cell Biology 2019Quote: ... these sequences were amplified by PCR from plasmids described above and inserted into SmaI-digested pLVX-puro vector (Clontech) by Gibson Assembly ...
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bioRxiv - Cell Biology 2019Quote: The rescued mEFs (expressing vimentin) were created by PCR amplification of the vimentin coding sequence using CloneAmp polymerase (Clontech) from pcDNA4- vimentin (provided by J ...
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bioRxiv - Cell Biology 2019Quote: ... The final cassettes were assembled by overlap extension PCR and used for In-Fusion cloning (Clontech, Palo Alto, USA) into linearized pTOR-Gateway vectors ...
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bioRxiv - Developmental Biology 2020Quote: ... and mCherry were isolated by PCR from various templates and inserted into the pGL4.23-(C120×5)-TATA vector with In-Fusion cloning (Clontech) according to manufacturer instructions using a 1:2 vector-to-insert ratio to generate optogenetic response plasmids ...
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bioRxiv - Cancer Biology 2019Quote: ... RT-PCR and cDNA synthesis were performed using SMARTer Ultra Low Input RNA Kit v3 following manufacturer’s protocol (Clontech). RNA isolated from 1000 cells was used as starting material ...
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bioRxiv - Cancer Biology 2019Quote: ... RT-PCR and cDNA synthesis were performed using SMARTer Ultra Low Input RNA Kit v3 following manufacturer’s protocol (Clontech). Single cells were used directly as starting material without RNA extraction to avoid extra loss of RNA ...