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Citations for Takara Bio :
1551 - 1600 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Genomic titer was determined using the Lenti-Z qRT-PCR Titration Kit according to manufacturer’s instructions (Takara #631235). Smaller scale virus prep for sgRNAs and shRNAs was performed through a similar transfection in a 12-well culture plate ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was carried out using the SYBR® Premix Ex Taq™ II (Takara Biotechnology, Dalian, China) and CFX96 Connect Real-Time system (Bio-RAD ...
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bioRxiv - Plant Biology 2021Quote: ... Mutations or deletions were introduced by PCR with PrimeSTAR® GXL DNA Polymerase (TaKaRa Bio Inc.; Catalog # R050A) using primers and DNA templates listed in Supplementary Table 4 ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were cloned into the modified pET28 vector using the In-Fusion HD Cloning Kit (Clontech) to generate pET28M-MpaKAI2A and pET28M-MpaKAI2B ...
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bioRxiv - Microbiology 2021Quote: ... and the cDNA was analyzed by qRT-PCR using TB Green® Premix Ex TaqTM II (Takara, Japan). The primer sequences are shown in Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... Real-time reverse transcription-PCR was performed using a Thermal Cycler Dice real-time system MRQ (Takara Bio) with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Biochemistry 2020Quote: ... OLS subpools corresponding to a given gene fragment were PCR amplified using Primestar GXL DNA polymerase (Takara Bio) according to the manufacturer’s instructions in 50 µl reactions using 1 µl of the OLS pool as the template and 25 cycles of PCR ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR-amplified DNA fragments were assembled and cloned into pET-in situ Ble plasmid by recombination (InFusion, Clontech) and the resulting cell line was named ABbin situ ...
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bioRxiv - Cell Biology 2022Quote: ... The qRT-PCR analysis was performed using the Takara Bio SYBR Premix Ex Taq (Takara, Cat. # RR420A, JPN) and CFX096 (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: Plasmids encoding human Ankrd26-GFP were cloned by PCR using EST clone 6830677 (NM_014915.2) as template and subcloned into pEGFP-N3 (Clontech) using KpnI und BamHI as restriction sites ...
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bioRxiv - Genomics 2022Quote: ... PCR amplification was performed for 8-9 cycles using high-fidelity polymerase (LA-Taq or PrimeSTAR GXL, Takara) at an annealing temperature of 60℃ ...
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bioRxiv - Plant Biology 2019Quote: ... 2.4 µg of clean RNA was reverse-transcribed to cDNA using Takara RNA PCR kit (Takara, Kyoto, Japan). Gene-specific primers (Table S3 ...
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bioRxiv - Neuroscience 2019Quote: ... Single stranded cDNA from the total RNA was synthesized with a RT-PCR kit (Clontech, Mountain View, CA) according to the kit’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... GST and AOC3/ZAG) was amplified by PCR and cloned into the expression plasmid pLVX-Tight Puro (Clontech), which allows packaging of constructs in a lentiviral format ...
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bioRxiv - Physiology 2019Quote: ... cDNA synthesis and PCR amplification were performed using PrimeScript First-Strand cDNA Synthesis kit (TaKaRa Bio, Tokyo, Japan) with 1 μg total RNA plus random hexamers ...
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bioRxiv - Bioengineering 2019Quote: Splinkerette PCR (spPCR) was done on genomic DNA from Klg-1 mESCs using Primestar HS mastermix (Takara R040) and primers specific for the inverse terminal repeats of the piggyBac sequences flanking the immunomodulatory transgenes ...
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bioRxiv - Cell Biology 2019Quote: ... VPS33A and VPS33B macronuclear ORFs were PCR-amplified and inserted by In-Fusion cloning (Clontech, Mountain View, CA) in the linearized p6c-myc-Chx vector at the SpeI site ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time quantitative RT-PCR was performed with a PrimeScript II 1st strand cDNA Synthesis Kit (TaKaRa Bio) and SYBR Premix Ex Taq II (TaKaRa Bio) ...
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bioRxiv - Genomics 2020Quote: ... full-length cDNA synthesis from poly-A containing transcripts was performed using the Clontech SMARTer PCR kit (Takara). The resulting cDNA was PCR amplified to generate 1-2ug cDNA ...
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bioRxiv - Genomics 2019Quote: ... The cDNA for reverse-transcriptase PCR is prepared using PrimeScript™ first strand cDNA Synthesis Kit from TaKaRa. The cDNA is appropriately diluted to proceed for analysis by quantitative PCR
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bioRxiv - Plant Biology 2021Quote: ... HyPRP expression (in WT/ACD6-1HA) was analyzed by quantitative RT-PCR using SYBR Premix Ex Taq (Takara) in a Bio-Rad Real-Time CFX96TM C1000 system (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Each 20 μL of PCR mixture contained 10μl of TB Green Premix Ex Taq (Tli RNaseH Plus, Takara) (2X) ...
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bioRxiv - Microbiology 2021Quote: ... The three PCR products were gel-purified and cloned into a linearized pUC19 by In-fusion cloning (Clontech) to obtain the template for homologous recombination (LarmDICER-FlagHAGFP-RarmDICER).
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bioRxiv - Genetics 2020Quote: The final stitching PCR was performed using primers csrL-f and csrR-r with LA Taq polymerase (Takara) in a 50 μl reaction ...
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bioRxiv - Cell Biology 2019Quote: ... All lentiviral constructs were cloned using PCR amplification of complementary fragments and In-Fusion HD-based assembly (Takara). ErkKTR-BFP was cloned by PCR amplification of TagBFP (Addgene 102350 ...
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bioRxiv - Genomics 2021Quote: ... Long range PCR was performed using Advantage 2 Polymerase following the manufacturer’s protocol (Clontech Laboratories, Mountain View CA).
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bioRxiv - Evolutionary Biology 2021Quote: ... Both missing ends could be recovered by RACE PCR (SMARTer RACE kit, Takara Bio, Mountain View, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... The transcript abundance was measured in 10 µL volume of the SYBR Green PCR Master Mix (Takara, Japan) containing cDNA corresponding to 5 ng of total RNA with gene-specific primers ...
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bioRxiv - Physiology 2021Quote: ... The PCR product was cloned into a pIRES2-EGFP expression vector (BD Biosciences Clontech Franklin Lakes, NJ, USA) in which the MluI site was inserted at the multi cloning site using the MluI and XhoI sites ...
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bioRxiv - Molecular Biology 2021Quote: ... pENTR1A SLX4 WT was used as a template and PCR was performed with the Advantage HD polymerase (Clontech). The PCR product was then digested by DpnI and subsequently phosphorylated and ligated using T4 PNK and T4 ligase (NEB ...
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bioRxiv - Genomics 2020Quote: First strand cDNA synthesis was performed on ~1μg RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) followed by PCR amplification with PrimeSTAR GXL DNA Polymerase (Clontech ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products then were inserted into a Tet-on inducible lentiviral vector pLVX (Takara, Mountain View, CA) through EcoRI and BamHI restriction sites ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted RNA was reverse transcribed using a PrimeScript RT-PCR kit with DNase I (Perfect Real Time) (Takara) in accordance with the accompanying protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... the PCR-amplified fragment was cloned into the NdeI and XhoI sites of the pCold-GST vector (TaKaRa).
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bioRxiv - Neuroscience 2020Quote: ... Target sites were amplified from tail genomic DNA by PCR using the Ex Taq Polymerase kit (Takara RR001A) with primers listed in Supplemental Table 2 ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were then isolated through gel electrophoresis and purified using the Nucleospin gel purification kit (Takara Bio). Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent E ...
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bioRxiv - Molecular Biology 2022Quote: ... a DNA fragment of AT1G13390 was amplified from Col-0 genomic DNA using CloneAmp HiFi PCR Premix (Clontech) with primers listed in Table S7 ...
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bioRxiv - Plant Biology 2022Quote: ... the full-length cDNA for PME17 was amplified by PCR using TakaRa Ex Taq DNA polymerase (TAKARA, Kyoto), and specific primers flanked by Gateway adapters ...
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bioRxiv - Physiology 2023Quote: ... Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus) (Takara bio RR820W) and a QuantStudio 6 Flex Real Time PCR system (Thermo Fisher ...
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bioRxiv - Genomics 2022Quote: ... Sublibraries were PCR amplified using primer-specific barcodes for each sublibraries and PrimeStar GXL DNA polymerase (Takara Bio) according to the manufacturer’s instructions in 50 µL reactions using 1µL of the total OLS library as template and 25 cycles of PCR ...
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bioRxiv - Physiology 2022Quote: ... The insulin-like growth factor 1 (IGF1) coding sequence was PCR amplified (CloneAmp, Takara Bio; Cat. No. 639298) from cDNA generated from a rat gastrocnemius muscle ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product thus obtained was cloned using the In-Fusion HD Cloning Plus kit (Takara Bio – 638909) into the pMA_F3NeoLoxPPolyFRT vector provided by Dr ...
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bioRxiv - Cell Biology 2022Quote: ... a DNA fragment was PCR-amplified from human genomic DNA using Tks Gflex DNA Polymerase (#R060A, Takara Bio) and the primers listed in Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... and in-chip reverse transcription PCR were performed using a 3’ DE Chip and Reagent kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and 1 µg was used for cDNA synthesis with the PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). Of the cDNA ...
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bioRxiv - Immunology 2023Quote: ... Real-time quantitative RT-PCR (qPCR) reactions were performed with TB Green Premix Ex Taq™ (Takara, RR420A) in 384-well plates in a QuantStudio™ 6 Flex Real-Time PCR System ...
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bioRxiv - Microbiology 2022Quote: ... and PCR inhibitors were removed from the DNA extracts using CHROMA SPIN+TE-400 Columns (Takara Bio, Inc.) and Amicon Ultra-0.5 (100K ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR amplification was typically conducted with ~20 overlapping DNA base primers and the PrimeSTAR HS DNA Polymerase (Clontech) or the Phusion Flash High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... MaV108 was transformed with a DNA fragment that was amplified by PCR reaction using a plasmid pLexA (Clontech) as a template for HIS3 marker ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara). Finally ...