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Citations for Takara Bio :
1801 - 1850 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... dual sgRNA cassettes and plasmid DNA (pDNA) were PCR-amplified and barcoded with sequencing adaptors using ExTaq DNA Polymerase (Clontech) following the same procedure as Supplementary Note 1 in Najm et al ...
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bioRxiv - Cell Biology 2021Quote: ... BicD and GFP CDS were amplified by PCR and the two fragments were combined into AttB-pUASp-K10 vector by InFusion cloning (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time qRT-PCR was performed as previously described using TB Green Premix Ex Taq II (Takara Bio Inc, Japan) and a QuantStudio 7 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: Cultured rat cortical neurons were infected with recombinant lentiviruses at DIV3 and harvested at DIV10 for qRT-PCR using SYBR green qPCR master mix (Takara). Total RNA was extracted from rat cortical neurons using the TRIzol reagent (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pJYB240 was generated from pJYB212 by exchanging the mEos2 open reading frame by a PCR-amplified mTurquoise2 DNA (Goedhart et al. 2012) using the InFusion kit (Clontech). The parBF-mTurquoise2 gene from pJYB240 was introduced into pJYB243 ...
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR products were cloned into a NsilI/SphI digested pSW-lacZ::sfGFP backbone by infusion cloning (Takara, Z9633N).
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bioRxiv - Neuroscience 2020Quote: Cultured rat cortical neurons were infected with recombinant lentiviruses at DIV4 and harvested at DIV11 for qRT-PCR using SYBR green qPCR master mix (TaKaRa). Total RNA was extracted from mouse cortical neurons using TRIzol reagent (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The viral load was measured by RT-qPCR using One-Step SYBR® Primescript™ RT-PCR kit II (Takara). CT values of serum samples were used to calculate serum viral titre according to regression equation built by RNA extracted from 10 µL of 102-106 pfu/mL of ZIKV (PRVABC59 or MP1751) ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting PCR product gel extracted and inserted into the XhoI/NheI-cut plasmid via In-Fusion Cloning (Takara) to make the donor vector pM2GT-1437000-mNeonGreen-3xHA ...
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bioRxiv - Neuroscience 2020Quote: ... The virus titer of purified AAV stocks was determined by AAVpro® Titration kit (for Real Time PCR) ver2 (TaKaRa).
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bioRxiv - Neuroscience 2020Quote: ... the 11kb genomic region encompassing kdm5 was amplified by PCR from w1118 and cloned into the pattB vector (Bischof et al., 2007) using the In-Fusion cloning system (Takara). A 3xHA tag was included in-frame at the 3’end of the kdm5 ORF ...
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bioRxiv - Immunology 2021Quote: ... [50]) coding for Drosophila SPARC was used as a template for PCR amplification using Takara Ex Taq polymerase (Takara Biomedicals) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... mIRE1-wt was PCR-amplified (without a myc tag) from pcDNA3-mIRE1-3xmyc (Stahl et al, 2013) and subcloned in pMSCVhyg (Clontech) using BglII and HpaI sites ...
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bioRxiv - Microbiology 2021Quote: ... Two ∼0.5□kb DNA fragments flanking the genes of interest were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara bio), M ...
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bioRxiv - Neuroscience 2020Quote: ... which was performed according to the manufacturer’s guidelines using the iCell8 Chip and Reagent Kit using in-chip RT-PCR amplification chemistry (Wafergen, Takara Bio) and Nextera XT DNA Library Preparation Kit and Nextera XT Index Kit (Illumina) ...
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bioRxiv - Cell Biology 2020Quote: ... 3C protease-cleavage site and a His12-tag at the N-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Plant Biology 2021Quote: ... captured DNA fragments were PCR-amplified using Takara LA Taq DNA Polymerase over 15 cycles (Clontech/Takara Bio, Otsu, Japan) then purified using AMPure PB Beads (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: ... The resulting product was purified from a 1% agarose gel using the NucleoSpin Gel and PCR Clean-up Kit (Takara) and Sanger sequenced.
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bioRxiv - Microbiology 2019Quote: ... PCR was conducted with tprK-specific primers (S1 Table) appended to 16bp PacBio barcodes using CloneAmp HiFi polymerase mix (Takara) and the following conditions ...
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment of the TkR86C coding region was amplified from cDNA from the Canton-S wild type strain by PCR (PrimeSTAR GXL, TaKaRa Clontech) with primers that had NotI and XbaI sites at 5’ and 3’ ends ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product and the pMECS phagemid vector were digested sequentially with Pst I and Not I (TaKaRa, Dalian, China) and then ligated by T4 DNA ligase at 16°C overnight ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... The complete sequence of CDK5RAP3 without the stop codon was excised from pCR-hCDK5RAP3 by NheI/SalI and ligated into pEGFP-N3 (Clontech), resulting in plasmid phCDK5RAP3-EGFP ...
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bioRxiv - Developmental Biology 2022Quote: ... 1µl diluted cDNA was used as the template for each reaction with Emerald Amp GT PCR Master Mix (Takara, RR310). After appropriate amplification cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA extracted and PCR genotyped with primers (Supplemental table 1) flanking each homologous arms using PrimeStar GXL DNA polymerase (Takara). Correctly targeted clones were further expanded and banked ...
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bioRxiv - Biochemistry 2022Quote: ... and the linearized plasmid gel-extracted and assembled with PCR-amplified codon-optimized mNeongreen or mScarlet using In-fusion cloning (Takara). To generate pPD95.75-itr- 1p-mNG or pPD95.75-itr-1p-mSc plasmids pPD95.75-mNG plasmid was digested with HindIII and PstI ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time RCR (qRT-PCR) was carried-out using SYBR Premix Ex Taq™ (Tli RNaseH Plus) (TaKaRa, Japan) and CFX96TM real-time PCR system (Biorad ...
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bioRxiv - Microbiology 2022Quote: ... covering the full-length SARS-CoV- 2 genome were amplified by PCR using a PrimeSTAR GXL DNA polymerase (TaKaRa Bio), the synthesized cDNA and specific primer sets from CoV-2-G1-Fw to CoV-2-G10-Rv designed previously (21) ...
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bioRxiv - Genetics 2022Quote: ... The protein-coding genomic sequence including 3 kbp promoter sequence was PCR-amplified and cloned into the pGreen0029 binary vector (Hellens et al., 2000) using In-Fusion Cloning system (Takara Bio Europe ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA gene sequences were amplified by polymerase chain reaction (PCR) using LA Taq polymerase (TaKaRa-Bio, Inc., Kusatsu, Japan) for Illumina MiSeq paired-end sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNAs were diluted 1/10 and PCR reactions were conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primers can be found in Supplementary Table 4 ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA was amplified via PCR and cloned into the appropriate vector backbones (empty pCS2+ or pCS2+ with C-terminal 3xeGFP) using InFusion Assembly (Takara). The GAP-dead mutant (RGA-3/4R80E ...
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bioRxiv - Cell Biology 2022Quote: pLVX-Puro GFP-TMEM11 was generated by PCR amplifying TMEM11 from human cDNA and cloning into the XhoI/BamHI sites of pAcGFP1-C1 (Takara), followed by subsequent cloning of the GFP-TMEM11 cassette into the Xho/BamHI sites of pLVX-Puro (Takara ...
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bioRxiv - Plant Biology 2021Quote: ... The CDS of the NON-RIPENING gene (NOR, Solyc10g006880) was amplified from the synthesized cDNA by PCR using PrimeSTAR GXL DNA Polymerase (TaKaRa) and the primer set SlNOR-pPLV26-F/R (Supplementary Table S11) ...
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bioRxiv - Microbiology 2020Quote: ... gene was then PCR amplified and purified then cloned into plasmid pLC29144 using In-Fusion HD Cloning plus kit (Takara). All PCR products and linearized vector were purified (Macherey and Nagel ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR reactions were performed using 1/60 of the immunoprecipitated DNA and the TB Green Premix Ex Taq II (Takara). Relative quantification using standard curve method was performed for each primer pair and 96-well Optical Reaction plates were read in a StepOnePlus PCR instrument (Applied Biosystem) ...
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A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: ... N-terminal containing TAD and C-terminal containing bHLH of PIF4 were PCR-amplified and fused with GAL4 activation domain (AD) of the pray vector pGADT7 (Clontech). Bait and pray vectors were co-transformed in to the Y2H Gold yeast strain ...
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bioRxiv - Genomics 2019Quote: ... The IsoSeq libraries were created starting from 1µg of total RNA per sample and full-length cDNA was then generated using the SMARTer PCR cDNA synthesis kit (Clontech) following PacBio recommendations set out in the IsoSeq method (pacb.com/wp-content/uploads/Procedure-Checklist-Iso-Seq-Template-Preparation-for-Sequel-Systems.pdf) ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using the THUNDERBIRD SYBR qPCR Mix (Toyobo) and the Thermal Cycler Dice Real Time System II (Takara). The transcript level in each strain was normalized to the internal control (rnpB) ...
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bioRxiv - Genomics 2021Quote: ... These nanowells were selected for subsequent targeted deposition of 50□nl/nanowell RT-PCR reaction mix from the SMARTer ICELL8 3’ DE Reagent Kit (Takara) using the MSND ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were conducted in 50-μL total volumes containing 1 μL of template DNA using an ExTaq kit (Takara). The cycling protocol was as follows ...
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bioRxiv - Plant Biology 2021Quote: Tagmented DNA was amplified directly from the beads via 6 cycles of PCR amplification using the PrimeSTAR GXL Polymerase kit (Takara) and dual indexed adapters (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The circularized DNA was subjected to PCR to generate a sequencing library using Illumina index primers and PrimeSTAR Max DNA polymerase (Takara). The PCR product was loaded and electrophoresed by 8% TBE PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... captured DNA fragments were PCR-amplified using Takara LA Taq DNA Polymerase over 15 cycles (Clontech/Takara Bio, Otsu, Japan) then purified using AMPure PB Beads (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... Detection of the number of copies of extracted RNA was performed using the Real-Time One-Step RT-PCR reagent (Takara). The following was the reaction system ...
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bioRxiv - Biochemistry 2020Quote: ... complementary BsmBI sites to those on the OLS fragments of a respective subpool were added by PCR using Primerstar GXL DNA polymerase (Takara) and 100 pg of wildtype channel as template DNA (Supplemental Figure S3A) ...
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bioRxiv - Microbiology 2021Quote: ... P3 and NP from D/660 and D/CN286 were amplified from the respective bidirectional pHW2000 plasmid constructs using CloneAmp Hifi PCR mix (Takara) with the following cycle profile ...
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bioRxiv - Plant Biology 2021Quote: ... and ΔRST (missing the residues 462-589) deletions were introduced by PCR using primers listed in Supplementary table 3 and end-joining using In-Fusion (Clontech).
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bioRxiv - Plant Biology 2021Quote: ... and ΔRST (missing the residues 462-589) deletions were introduced by PCR using primers listed in Supplementary table 3 and end-joining using In-Fusion (Takara).
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bioRxiv - Microbiology 2022Quote: ... A second construct for total gene deletion was generated by cloning the 5’ (843 bp) and 3’ (292 bp) homology regions amplified by PCR using a Taq DNA polymerase with proofreading activity (Takara) and primers P9 + P12 and P13 + P14 into the pL0001 vector (BEI Resources ...
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bioRxiv - Immunology 2022Quote: ... was cloned from pHRSIN (kind gift from Prof. Alain Townsend) into the Addgene 17448 lentivirus vector by InFusion PCR (TaKaRa) and verified by Sanger sequencing ...