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Citations for Takara Bio :
1501 - 1550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... HEK-293 cells were transfected with the expression vector and secreted protein was purified by affinity chromatography (His60 resin, Clontech) and size exclusion chromatography (26/60 Superdex 200 HiLoad (GE) ...
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bioRxiv - Cell Biology 2024Quote: InFusion-HD Cloning System (Takara Bio USA, Inc., #638946)
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bioRxiv - Microbiology 2024Quote: ... The rbp3 gene (slr0193) was amplified and ligated into vector pGEX-6P-1 using the In-Fusion Cloning kit (TaKaRa, Shiga, Japan) and the PCR primers pGEX-6P-1_inv-f/-r and Rbp3_iVEC-f/-r (SI Appendix ...
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bioRxiv - Microbiology 2024Quote: ... was reverse-transcripted using PrimeScript II 1st Strand cDNA Synthesis Kit (TAKARA, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from intestinal tissues by RNAiso Plus kit (TAKARA, Dalian, China) was reverse-transcripted using PrimeScript II 1st Strand cDNA Synthesis Kit (TAKARA ...
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bioRxiv - Microbiology 2024Quote: ... the infected RAW 264.7 cells were collected using RNAiso Plus (TaKaRa, Dalian, China) for total RNA extracting and quantitative real-time PCR (qPCR) ...
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bioRxiv - Microbiology 2024Quote: ... which detects GFP in our study (Clontech, 63281; 1:5000), goat anti-rabbit IgG secondary antibody (Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Homology-directed repair templates were PCR amplified using the CloneAmp™ HiFi PCR Premix (Takara Bio, 639298). Guide RNA and repair template sequences are listed in Table S1.
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CTCF regulates global chromatin accessibility and transcription during rod photoreceptor developmentbioRxiv - Genomics 2024Quote: ... and 1 ng total RNA was used to generate RNA-seq libraries with SMARTer Stranded Total RNA-Seq Kit v3 (Takara). All samples were sequenced with NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using PrimeScript RT reagent Kit (TaKaRa). The mRNA levels of the sehA ...
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bioRxiv - Genetics 2024Quote: ... The cells were then harvested and genomic DNA was extracted using NucleoSpin Blood XL kit (Clontech). Libraries were prepared and sequenced as previously described 4.
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bioRxiv - Genomics 2024Quote: ... 0.8 mM dNTP mix and 2 U Ex Taq polymerase (TaKaRa, Otsu, Japan). The thermal profile of the reaction started with initial stage 90 s at 94 °C which was followed by 30 cycles of 45 s denaturation at 94 °C ...
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bioRxiv - Genomics 2024Quote: ... 1x Ex Taq buffer and 2 U Ex Taq polymerase (TaKaRa, Otsu, Japan). The thermal profile started with initial stage 94 °C for 3 min which was followed by 30 cycles of 1 min denaturation at 94 °C ...
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bioRxiv - Genetics 2024Quote: ... After transformation into Stellar competent cells (Clontech, 636766), combining five replicates in order to maximise the number of individual transformants amplified ...
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bioRxiv - Genetics 2024Quote: ... 1 μg of total RNA was reversely transcribed into cDNA using the Prime Script RT kit (Takara, RR047A). The SYBR Green qPCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... Backbone preparation was run in 4x of 50 μL PCR using PrimeSTAR Max from Takara as described above ...
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bioRxiv - Genomics 2024Quote: ... High molecular weight gDNA extractions for samples M20 and M29 were generated using the NucleoBond HMW DNA kit (740160.20) from Takara Bio USA ...
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bioRxiv - Genomics 2024Quote: Gastruloids were generated as described before18,21 with the following adaptations: N2B27 medium (Takara, #Y40002) was passed through a 0.22 μm filter before use and 600 cells were used for aggregation instead of 300 ...
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bioRxiv - Genomics 2024Quote: ... The subsequent steps for library preparation were carried out with the DNA Thruplex kit (Takara, Cat. #R400674, Cat. #R400665) following the manufacturer’s protocols.
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bioRxiv - Immunology 2024Quote: ... Complementary DNA was synthesized using the PrimeScript RT Reagent Kit (RR037, Takara Bio). qPCR was performed using TB Green PCR Master Mix (RR820 ...
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bioRxiv - Microbiology 2024Quote: Tissue samples were collected and total RNA was extracted using Trizol (Takara, CA, JPN) as previously described ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli strain DH5α (Takara Bio, Shiga, Japan) was used as a host for the plasmid derivatives ...
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bioRxiv - Microbiology 2024Quote: ... Then dual-indexing library preparation was created in equimolar conditions following the SMARTSeq Library Prep kit (Takara Bio) and Illumina sequencing 2x 150 reads was done on a Novaseq SP by Fasteris-GeneSupport SA (Switzerland) ...
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bioRxiv - Microbiology 2024Quote: ... Typhimurium SpvB (residues 367-591) was similarly cloned into a modified72 pCold(I) plasmid (Takara). The VopF fragment encoding amino acid residues 129-530 containing the three WH2 domains and the VopF C-terminal actin-binding domain (VCD ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using PrimeScript RT Reagent Kit (TaKaRa Bio Inc., Shiga, Japan). The qRT-PCR assays was performed using Luna Universal qPCR Master Mix (New England BioLabs ...
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bioRxiv - Immunology 2024Quote: ... A Lenti-X concentrator (Takara Bio USA) was used according to the manufacturer’s protocol to concentrate the virus 10-20X and the resulting lentiviral stocks were aliquoted and stored at −80°C ...
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bioRxiv - Immunology 2024Quote: ... Total RNA from cultured cells or spleens was extracted using RNAiso Plus (Takara Bio). Total RNA from mouse whole blood was isolated using PAXgene Blood RNA Tubes and kit (761265 ...
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bioRxiv - Microbiology 2024Quote: ... These fragments were further cloned with In-Fusion cloning (Takara Biotech) downstream of the polyubiquitin promoter into the pPub plasmid [48 ...
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bioRxiv - Immunology 2024Quote: ... RNA samples were collected using RNAiso Plus (9108, Takara Bio).
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bioRxiv - Genomics 2024Quote: ... Lentiviral particles were produced in HEK293T Lenti-X cells (Clontech, Mountain View, CA). Cells (5×106 ...
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bioRxiv - Immunology 2024Quote: ... The cDNA of shp-1 was obtained by reverse transcription of liver total RNA and subcloned into Hind III/BamH I (Takara, Japan) sites of pcDNA3.1EGFP vector ...
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bioRxiv - Immunology 2024Quote: ... The DNA encoding nfκb1 synthesized by Shanghai Genomics Institute was subcloned into Hind III/Xho I (Takara, Japan) sites of pcDNA3.1-3xFlag- C vector ...
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bioRxiv - Immunology 2024Quote: ... Purified DNA was transfected into nearly confluent (80-90%) 10 cm2 plates of low passage LentiX 293T (Takara #632180) using a calcium phosphate transfection protocol ...
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bioRxiv - Microbiology 2024Quote: ... using SYBR PremixEx TaqII (TAKARA). The primer sets are listed in Table S6 ...
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bioRxiv - Genomics 2024Quote: ... anti-Myc (Takara Bio), and anti-Flag (Sigma)] and 15 µl Dynabeads protein G (Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The rRNA genes (18S, ITS, 28S) were amplified by polymerase chain reaction (PCR) using the TaKaRa Ex Taq polymerase kit (TaKaRa Bio-medicals, Seoul, Korea). PCR cycling parameters and primer information were followed as in Shazib et al ...
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bioRxiv - Cell Biology 2024Quote: ... or pCS2-6 × Myc expression vector (Clontech, Mountain View, CA, USA). All plasmids were verified by sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... using TRI reagent (TAKARA) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA iso Plus (TaKaRa), 6X loading Dye (Fermentas) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated from 500ng of RNA using PrimeScript RT Master Mix (Takara, Cat. # RR036B). The cDNA was diluted 1:20 and 1µl of the diluted cDNA was used for qPCR analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Viral supernatants were harvested at 48 and 72 hours post transfection and concentrated with lenti-X concentrator solution (Clontech). TRE-MYC HCECs were infected in triplicate at a low MOI (0.2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Expression of cDNAs was induced with 100 ng/mL doxycycline (Clontech/Takara). Site-directed mutagenesis was used to generate the citrate binding mutant SLC25A1 ...
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bioRxiv - Cancer Biology 2024Quote: ... it was concentrated 100-fold from cell culture medium using Lenti-X™ Concentrator (Takara, #631231) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was further diluted for LDH detection according to the product manual (MK401, Takara). Briefly ...
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bioRxiv - Systems Biology 2024Quote: ... by transfection and concentrated using Lenti-X concentrator (Takara Bio, Cat# 631231). We transduced NPCs with the concentrated lentiviruses and selected the transduced NPCs using 4ug/ml puromycin until the BFP+ cells reached greater than 90% ...
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bioRxiv - Systems Biology 2024Quote: ... Lentiviruses carrying sgRNAs were produced in Lenti-X 293T cells (Takara Bio, Cat#632180) by transfection and concentrated using Lenti-X concentrator (Takara Bio ...
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bioRxiv - Plant Biology 2024Quote: ... Purification of the His6-tagged protein was achieved by TALON® spin columns (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cDNA was reverse transcribed by using a PrimeScript RT Reagent Kit Perfect Real Time (TaKaRa Bio Inc.). Real-time PCR was performed using a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Expression of cDNAs was induced with 100 ng/mL doxycycline (Clontech/Takara). Site-directed mutagenesis was used to generate the citrate binding mutant SLC25A1 ...
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bioRxiv - Cell Biology 2024Quote: ... The PrimeScript RT Master Mix Kit (TaKaRa) was used to generate cDNA from the extracted RNA ...