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Citations for Takara Bio :
1551 - 1600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Supernatant was incubated with TALON beads (Takara Clontech) for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... [61] digested with EcoRI/XhoI by In-Fusion HD Cloning Kit (Takara, 639649). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatant was incubated with TALON beads (Takara Clontech) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Both cDNAs were cloned into pIRES (Clontech) for their coupled expression from bicistronic mRNA to achieve near-quantitative Shh palmitoylation 23 ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA synthesis was performed using PrimeScript 1st strand cDNA Synthesis Kit (TakaRa, 28704) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µL of the eluate was digested overnight at 37°C with 10 units of DpnI (Takara, 1235A) in a final volume of 10 µL ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus) (#RR42WR, Takara Bio) with a final forward and reverse primer concentrations of 1 µM and cDNA concentrations of 2.5 ng/µl ...
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bioRxiv - Microbiology 2024Quote: Total RNA was isolated at specific time points post infection by using TRIzol (Takara) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The standard curve was prepared using the positive control solution provided by Takara with serial dilution for absolute quantification ...
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bioRxiv - Immunology 2024Quote: ... RNA was reverse transcribed using RNA to cDNA Ecodry premix (Takara Bio, Kusatsu, Japan). Gene expression was measured using Power SYBR Green PCR Master mix (Life Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... and ptdTomato-C1 (Clontech), respectively ...
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bioRxiv - Synthetic Biology 2024Quote: ... prior to insertion in the cloning vectors pEGFP-C1 (Clontech) and ptdTomato-C1 (Clontech) ...
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bioRxiv - Biochemistry 2024Quote: Construct preparation – Mammalian expression plasmids encoding fluorescent nanobody constructs were created using InFusion cloning (Takara Bio). DNA fragments encoding the open reading frame (ORF ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... The proteins were purified on a TALON Cell-Thru His-tag affinity column (Clontech) in a buffer containing 10 mM Na Phosphate (pH 7.0) ...
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bioRxiv - Biochemistry 2024Quote: ... anti-mCherry (#Z2496N, TaKaRa, Shiga, Japan) anti β-Actin (#bs-0061R ...
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bioRxiv - Biochemistry 2024Quote: ... TALON® metal affinity resin (500 μl, Takara Bio, Cat#635501) was equilibrated by resuspending the beads twice in 10 ml of binding buffer ...
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bioRxiv - Biochemistry 2024Quote: Anhydrotetracycline (aTc, TaKaRa, 631310), ATPγS (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... Arf1-EGFP and Arf1-mCherry plasmids were obtained by subcloning Arf1 (Wessels et al., 2006) into pEGFP-N3 (Clontech) and pmCherry-N1 (Clontech) ...
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bioRxiv - Biochemistry 2024Quote: ... YatalaseTM Enzyme (Takara Bio), and lyticase from Arthrobacter luteus (Sigma Aldrich) ...
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bioRxiv - Genomics 2024Quote: ... the first PCR was performed using a Multiplex PCR Assay Kit Ver.2 (Takara) with MIG-seq primer set-1 developed by Suyama and Matsuki (2015) ...
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bioRxiv - Genomics 2024Quote: ... which was performed with PrimeSTAR GXL DNA Polymerase (Takara) following the PCR program of Nishimura et al ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using the PrimeScript RT-PCR kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using TB Green Premix Ex Taq Kit (Takara) on QuantStudio 6 Flex Real-Time PCR System (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... Full-length cDNA was generated using the SMART-Seq® HT Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were transduced using lentivirus at an MOI between 5 and 10 on Retronectin-coated (Takara, T100B) plates overnight to express an M5 or SS1 CAR ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted using RNAiso plus (Takara, Japan). RNA (1 μg ...
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bioRxiv - Immunology 2024Quote: ... and converted to cDNA by using PrimeScript RT Master Mix (Takara), following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... Dync2li1-shRNA and Control-shRNA cassettes were subcloned into the pBAsi-mU6 vector (Takara Bio, Shiga, Japan). The target sequences were as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... 33 μg/mL retronectin (Clontech) and 2.67 μg/mL of DL1-extracellular domain fused to human IgG1 Fc protein (a gift from I ...
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bioRxiv - Immunology 2024Quote: ... following the manufacturer’s protocol for in-fusion cloning (Takara Bio). All heavy chains were similarly cloned into IgG1 and IgM plasmids in parallel ...
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bioRxiv - Microbiology 2024Quote: ... coli cells (Takara Bio) and plated on LB agar plus carbenicillin ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA cloning libraries were created using SMARTScribe™ Reverse Transcriptase (Takara/Clontech Cat # 639537) and the Advantage 2 PCR kit (Takara/Clontech Cat # 639207) ...
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bioRxiv - Molecular Biology 2024Quote: ... digested pLG plasmid by In-Fusion® HD Cloning Kit (Takara) resulting in the pLd2G vector ...
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bioRxiv - Biochemistry 2024Quote: A pull-down assay was performed using a Capturem His-Tagged Purification Miniprep Kit (#635710, Takara, Shiga, Japan). 50 µg of BSA was added to the column for blocking ...
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bioRxiv - Microbiology 2024Quote: ... cDNA levels of the phoP and eptB genes were quantified by PCR using SYBR Premix Ex Taq II (Takara, Japan) solution containing RT-PCR primers for each gene (See Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... All RNAs in the samples were converted into cDNA using a cDNA EcoDry Premix (Clontech, USA). cDNA levels of the phoP and eptB genes were quantified by PCR using SYBR Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2024Quote: RNAiso Plus (TAKARA) is used for RNA isolation ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane was then incubated with 1:1000 mouse anti-His6 antibody (Clontech) for 15 mins ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Site-directed mutagenesis was performed in the seed sequences of Mecp2 using PrimeStar Max DNA Polymerase (R045, Takara, Japan) for PCR amplification ...
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bioRxiv - Biochemistry 2024Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio). The sequences of all primers in this study can be found in Table S3 ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was reverse transcribed to cDNA with M-MLV reverse transcriptase kit (Takara). LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR in the presence of SYBR Green (TaKaRa TB Green Primer Ex TaqII, #RR820Q) was carried out according to the manufacturer’s instructions in a BioRAD CFX 384 Thermal Cycler (BioRAD ...
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bioRxiv - Neuroscience 2024Quote: ... or 10 µg DsRedExpress (Clontech) together with 1-2 µg of GFP-tagged PSD95α41 or PSD93α42 ...
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bioRxiv - Neuroscience 2024Quote: ... PCR was performed using Phusion (Takara), following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... were purchased from Thermo Fisher Scientific. cDNA synthesis kit (cat no. 6110B) and real-time PCR kit (cat no. PN 4367659) were purchased from TAKARA. ECL reagent (cat no ...
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bioRxiv - Physiology 2024Quote: ... S1A) using the In-Fusion HD system (Clontech, #639650) (19) ...
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bioRxiv - Neuroscience 2024Quote: ... D9891)-inducible adenoviral constructs were generated for Halo-TRIM9 and UNC5C-pHmScarlet using the adeno-X™ system 3 (Takara Bio, 631180), using the detailed protocol outlined by (O’Shaughnessy et al. ...
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bioRxiv - Neuroscience 2024Quote: ... and a total of 0.9 μg of cDNA vectors encoding the GluN1/GluN2 receptors and GFP (to identify the transfected cells; pQBI 25, Takara, Shiga, Japan). After transfection ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was transfected into nearly confluent (80-90%) 10 cm2 plates of low-passage LentiX 293T (Takara #632180) using a calcium phosphate transfection protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic titer was determined by Lenti-X GoStix Plus (Takara). Lentivirus was functionally titrated previously using parallel preparation of GFP-expressing viral particles (Campeau et al. ...