Labshake search
Citations for Takara Bio :
1451 - 1500 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Epidemiology 2021Quote: ... and SYBR Premix Ex Taq Kit for real-time PCR assay were purchased from Takara (Dalian, China). Monoclonal CD3-FITC/APC ...
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bioRxiv - Genomics 2020Quote: cDNA was synthesized from parasite RNA using the SMARTer PCR cDNA synthesis kit (Takara, Mountain View, CA), which incorporates a poly(A ...
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bioRxiv - Immunology 2020Quote: Quantitative real-time PCR was conducted to quantify the mRNA levels using Premix Ex Taq (Takara, Japan). Total RNA was extracted from mud crab hemocytes ...
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bioRxiv - Immunology 2020Quote: ... the DNA-amplicons for Illumina sequencing were generated by using Advantage 2 PCR Kit (TAKARA Bio Europe), employing a mTCRβ reverse primer and a Universal primer mix (UPM) ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed using SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara) and complementary DNA was synthesized with a PrimeScript™ RT reagent Kit with gDNA Eraser (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... was amplified and linearized via PCR using the high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio, Japan). In the PCR product ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested with XhoI/BamHI and cloned into XhoI/BamHI sites of pGal4 (Clontech). For C-terminal GFP-fusion of Pex25 ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA from patients was amplified by Polymerase chain reaction (PCR) using PrimeSTAR HS Premix (Takara Bio) on a Mastercycler nexus (Eppendorf ...
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bioRxiv - Immunology 2022Quote: ... After the optimization of PCR cycle number using SYBER Green I Nucleic Acid gel Stain (Takara Bio), transposed fragments were amplified using NEBNext High Fidelity 2× PCR Master mix and index primers ...
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bioRxiv - Developmental Biology 2022Quote: ... Genome fragments of each AQP gene were PCR-amplified using MightyAmp DNA polymerase ver.3 (Takara Bio) with the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... PCR amplification of the RNA-Seq library was performed using SeqAmp DNA Polymerase (Takara Bio, cat# 638509). Each library was then purified prior to sequencing using SPRI AMPure Beads (Beckman Coulter) ...
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bioRxiv - Immunology 2022Quote: ... qRT-PCR was performed using the SYBRGreen included in the BacPAK™ qPCR titration kit (Clontech, USA) and a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: ... Relative quantitative PCR was conducted with a TB Green Primer Ex Taq II (Takara Bio Inc., Japan) using LightCycler® 96 Real-Time PCR System (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was subjected to relative quantitative PCR using the SYBR Premix Ex Taq kit (TaKaRa) on a Roche LightCycler 480 real-time PCR machine ...
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bioRxiv - Synthetic Biology 2022Quote: ... digested with appropriate restriction enzymes utilizing In-Fusion PCR cloning technology (Clontech Laboratories, Inc., Mountain View, CA). Cloning inserts were created via PCR of ORFs of interest from their respective genomic (all E ...
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bioRxiv - Cell Biology 2022Quote: ... Error-prone PCR of rec8+ cDNA in VP068-GFP-BsdR by Ex Taq DNA Polymerase (TaKaRa Bio) was performed using several concentrations of MnCl2 (final 0.05 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... The in vitro assembly of PCR products was performed by using In-Fusion HD cloning kit (Takara). The assembled plasmids were amplified by transformation of Stellar Competent Cells (Takara ...
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bioRxiv - Pathology 2022Quote: ... Quantitative real-time PCR was performed by TB Green Fast qPCR Mix (Takara Bio Inc. Kusatsu, Japan) on a PCR System (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... and were routinely tested for mycoplasma contamination (assayed with PCR Mycoplasma Detection Set, TaKaRa, Cat. no. 6601) and pluripotency status with antibodies against OCT4 (rabbit ...
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bioRxiv - Neuroscience 2022Quote: ... were amplified by PCR and inserted into a CAG-promoter plasmid backbone by In-Fusion HD (Takara). A plasmid encoding hWDR62-FLAG was generated by amplifying hWDR62-FLAG by PCR ...
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bioRxiv - Molecular Biology 2023Quote: Microscopy constructs: NbV5-eGFP was generated by PCR amplification of NbV5 and cloned into pEGFP-N1 (Clontech) at the HindIII-BamHI sites.
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bioRxiv - Microbiology 2022Quote: ... All PCR reactions were performed on a Thermal Cycler Dice Real Time System III (Takara Bio, Inc.). Previously sequenced E ...
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bioRxiv - Neuroscience 2022Quote: ... Pgcl2 and Pgcl3 cDNAs in male rat livers were amplified by PCR (PrimeSTAR DNA Polymerase Takara, Japan). The oligonucleotide primer sequences employed for this purpose are shown in Supplementary Table S1 ...
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bioRxiv - Zoology 2023Quote: ... PCR products were resolved by agarose gel electrophoresis with the DL2000 DNA Ladder (TaKaRa Bio, Dalian, China) before bands of expected sizes were excised to remove primer dimers ...
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bioRxiv - Molecular Biology 2022Quote: ... The initial outer PCR was performed with TaKaRa ExTaq® DNA Polymerase Hot-Start Version polymerase (Takara) using forward (FW ...
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bioRxiv - Cell Biology 2022Quote: ... PRR14-GFP 1-212 PCR products were sub-cloned into pLVX-TetOne-Puro (Takara Bio, cat# 631847) vector using T7 ligase (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... NPAS2 mRNA was quantified by qRT-PCR with SYBR Premix ExTaq (Applied Takara Bio, Baori Medical Biotechnology) and normalized to GAPDH as the reference gene.
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bioRxiv - Molecular Biology 2023Quote: ... For quantitative RT-PCR 1ug of total RNA was treated with 5 Units of DNase I (Takara) and cDNA synthesized with SuperScriptIII (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... A vector was constructed by fusing three PCR amplicons as described below using In-fusion cloning (Takara) and used for homologous recombination ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab5A (pJV0054) was generated similarly: cDNA was cloned via PCR using primeSTAR max DNA polymerase (Clontech). This was followed by subcloning into a zero blunt TOPO II vector (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... These PCR products were digested by SalI and NotI and subcloned in frame into pCMV2-HA (Clontech) cut by the same enzymes ...
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bioRxiv - Immunology 2023Quote: Quantitative RT-PCR was performed with a SYBR Premix Ex Taq II (Tli RNaseH Plus) (RR820; Takara) on a 7500 Real-Time PCR system (Thermo Fisher) ...
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bioRxiv - Physiology 2023Quote: ... The qRT-PCR was conducted in a 20μl reaction volume employing SYBER Green Master Mix (TaKaRa, Japan). The qRT-PCR was carried out using the Applied Biosystems QuantStudio3 real-time PCR machine (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Semi-quantitative real-time PCR was performed with SYBR Premix DimerEraser (Perfect Real Time, TAKARA BIO Inc.) and a StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... First-strand cDNA synthesis was performed on 200ng RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech) with specific oligo(dT ...
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bioRxiv - Developmental Biology 2023Quote: ... the DNA regions of interest were PCR amplified in two successive reactions using HS Taq (Takara, R007). The first PCR amplified a larger fragment of arnt1 (about 700 bp ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reversely transcribed into cDNA using the PimeScript RT-PCR kit (TAKARA) and analyzed by qPCR on LightCycler96 system (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA libraries were constructed using the immunoprecipitated mRNA and SMARTer PCR cDNA Synthesis Kit (Clontech, Cat: 634926), and sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Microbiology 2023Quote: Amplification and detection were performed using the One Step PrimeScript™ RT-PCR Kit (RR064A, Takara Bio) with the following primers targeting the IAV M segment ...
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bioRxiv - Cancer Biology 2022Quote: ... Then real-time quantitative PCR was performed using TB Green® Premix Ex Taq™ II (Takara) on the Real-Time PCR Thermal Cycler qTOWER3 (Analytik Jena) ...
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bioRxiv - Microbiology 2023Quote: ... Genotyping PCR reactions were performed on 10ng of isolated parasite DNA using CloneAmp HiFi polymerase premix (Takara) in an Applied Biosystems thermocycler according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... DNA in the supernatant was purified using the NucleoSpin Gel & PCR kit (Takara Bio, Kusatsu, Shiga, Japan). Sequencing libraries were then prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time quantitative PCR was performed with TB Green® Premix Ex Taq™ II (TAKARA, Japan) using a Bio-Rad CFX 96 real-time PCR detection system (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... Polymerase chain reaction (PCR) was carried out using PrimeSTAR® GXL DNA Polymerase (TAKARA-Bio, Cat# R050). The PCR conditions were specific to each sequence and amplified using 10 μM sequence-specific paired oligonucleotides (see Supplementary Table 5 ...
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bioRxiv - Plant Biology 2023Quote: ... Primers flanking the region targeted for replacement were designed and used for Terra Direct genotyping PCR (Clontech) to identify transformants lacking the wild-type fragment ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Linearized pMK1334 vector was purified using NucleoSpin Gel and PCR clean-up kit (Takara Bio, Cat#740609.50). Top and bottom oligos of non-targeting and targeting sgRNAs were annealed ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA synthesis was performed using a one-step PrimeScript RT-PCR kit (Takara Bio, Cat. RR057B, Japan) as per the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... Resulting linear amplification products were pooled and purified using the NucleoSpin Gel and PCR Purification kit (Takara) with modified protocols for ssDNA (1:2 NTI buffer dilution) ...
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bioRxiv - Microbiology 2023Quote: ... using the Takara One Step TB Green PrimeScript™ RT-PCR kit (Takara Bio Inc., Shiga, JP) and the Roche LightCycler® 96 real-time PCR instrument (Roche Applied Science ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-PCR was performed using diluted cDNA (1:5 in water) and PrimeStar DNA Polymerase (Takara, R010A) with primers and PCR conditions listed in Table 1 ...