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Citations for Takara Bio :
1451 - 1500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... GFP (Living Colors, Takara Biotech, San Jose CA, 632375; 1:2000), Pfn1 (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... protocol with the P5 forward primer mix (Takara) and a unique reverse P7 primer for each condition ...
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bioRxiv - Cell Biology 2024Quote: ... The guides were then PCR amplified for 26 cycles using the Ex Taq polymerase (Takara) protocol with the P5 forward primer mix (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... were transfected with the Guide-it library (Takara) according to the manufacturer’s instructions and concentrated ∼100x using the Lenti-X concentrator kit (Takara ...
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bioRxiv - Physiology 2024Quote: 5’ and 3’ RACE assays were performed using a SMARTer RACE kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was reverse transcribed using a PrimeScript™ RT Reagent Kit (Takara Bio Europe), and PCR reactions were performed using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... according to the manufacturer’s instructions and concentrated ∼100x using the Lenti-X concentrator kit (Takara) and stored at −80°C until needed ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were digested using Dpn I (Takara, cat. no. 1235A) for 2 hours at 37 °C and transformed into Trans5 α bacterial chemically competent cells (TransGen Biotech ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles were collected 72 hours after transfection and concentrated by using Lenti-X Concentrator (631231, Takara). After transduction ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of produced viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Neuroscience 2024Quote: ... 1:500 (Clontech, 632496); Mouse Anti-GFP-4C9 ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μg of RNA was reverse transcribed using PrimeScript™ RT reagent kit (Takara, San Jose, CA), according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used in this study include Rabbit-anti-DsRed (Clontech #632496, 1:1000), Goat anti-GFP (Sicgen ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was prepared using NucleoSpin® RNA Plus XS (Clontech, cat# 740990). RNA was prepared from 4 mm SC sections at 5 dpi in tcima/b-/- and wild-type controls for bulk RNA sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech cat# 634936) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech cat# 634936) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Titers were obtained on freeze-thawed aliquots using the qPCR-based TaKaRa lentivirus titration kit (#631235, Takara Bio, Kusatsu, Shiga, Japan). Minimum titers for all in vivo experiments was 1×1011 genome copies/mL ...
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bioRxiv - Neuroscience 2024Quote: ... QUAS-tdTomato-NLS plasmid was cloned using Infusion HD Cloning Kit (Takara) and sent to Genetivision Corporation for injection ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (1:500, Takara Bio, 632496), and mouse anti-Brp (1:20 ...
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bioRxiv - Plant Biology 2024Quote: ... Bait plasmids were introduced into the Y2HGold yeast strain (Takara) using the lithium acetate method (Gietz and Schiestl 2007) ...
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bioRxiv - Plant Biology 2024Quote: ... the corresponding promoters (ampflied using primer listed in Table S7) were inserted into a modified pUC19 entry vector (Andersen, et al., 2021) via Infusion cloning (Takara) according to the manufacturer description ...
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bioRxiv - Plant Biology 2024Quote: ... PCR amplification was performed using Advantage® Genomic LA Polymerase Mix from TaKaRa Bio USA (Mountain View ...
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bioRxiv - Plant Biology 2024Quote: ... from plasmids pFny209 (see below) or pGFP-C1 (Clontech laboratories, Mountain View, CA), into the BpiI-digested modified pUC18 ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was performed using TB Green Premix Ex Taq II (TaKaRa Bio, Japan) on a StepOne Real-time PCR (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... These fragments were joined using an In-Fusion HD Cloning Kit (TaKaRa Bio, Japan). The primers used in this study are shown in Table 1.
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bioRxiv - Plant Biology 2024Quote: ... EjCDPK25 was cloned into pCAMBIA1301 vector using In-Fusion HD Cloning Kit (Takara). After that ...
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bioRxiv - Plant Biology 2024Quote: ... while AvrPwt4 and VirPwt4 were cloned into the bait vector pGBKT7 (Takara, #630443). These constructs were co-transformed into the yeast strain AH109 following the manufacturer’s instructions from Frozen-EZ Yeast Transformation II™ (Zymo Research) ...
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GIBBERELLIN SIGNALING THROUGH RGA SUPPRESSES GCN5 EFFECT ON STAMEN ELONGATION OF ARABIDOPSIS FLOWERSbioRxiv - Plant Biology 2024Quote: ... The PrimeScriptTM 1st strand cDNA synthesis kit (Takara, Shiga, Japan) was used for reverse transcription ...
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bioRxiv - Genomics 2024Quote: ... Plasmid modifications were generated by successive rounds of PCR using PrimeSTAR by Takara following manufacturer protocols ...
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bioRxiv - Cell Biology 2024Quote: ... was cloned into a pRetroQ (Clontech)-based retroviral vector ...
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bioRxiv - Cell Biology 2024Quote: ... HEK237T cells were obtained from Takara Bio (#632273 ...
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bioRxiv - Cell Biology 2024Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction of the supernatant was purified using TALON metal affinity resin (TAKARA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... PCR amplification was performed using SapphireAmp Fast PCR Master Mix (TAKARA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was purified and digested with nuclease P1 (Wako) and bacterial alkaline phosphatase (BAP; TAKARA, Shiga, Japan). The resulting modified nucleosides were exogenously added to cells ...
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bioRxiv - Cell Biology 2024Quote: ... and subcloning into the EGFP-N1 (Clontech) plasmid using NheI/SalI restriction sites ...
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bioRxiv - Cell Biology 2024Quote: ... AcrIIA4 was cloned into pENTR dTOPO and SMASh or dTAG domains were inserted via Gibson assembly (Clontech, cat. # 638909) to be in frame with AcrIIA4 ...
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bioRxiv - Cell Biology 2024Quote: ... vector using In-Fusion® HD Cloning Kit (Takara Bio USA, Inc). Primer sequences used were as follows ...
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bioRxiv - Cell Biology 2024Quote: EGFP-Actin (Clontech cat #6116-1). Apple ...
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bioRxiv - Cell Biology 2024Quote: ... and reverse-transcribed using the cDNA Synthesis kit (Takara, Japan). The RNA quantity and density were verified by a spectrophotometer ...
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bioRxiv - Cell Biology 2024Quote: ... The plasmids encoding truncated PARP2 or XRCC1 were generated via PCR-mediated mutagenesis and subcloned into the pEGFP-C1 and mRFP-C1 vectors (Clontech), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... with 10% FBS (Clontech) and 1% penicillin- streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by infusion cloning (Takara, 638911) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5-1.0 μg total RNA was used for cDNA synthesis with 5x PrimeScript RT Master Mix (Takara, RR036A-1). Quantitative real-time PCR was carried out using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: The plasmids used for transient overexpression were constructed using the pCMV-FLAG/Myc vector (Clontech). Point and deletion mutants were engineered utilizing the KOD-Plus-Mutagenesis Kit (TOYOBO ...
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bioRxiv - Cell Biology 2024Quote: ... where RapA(wt) was amplified from AX2 cDNA and inserted into pGBKT7 (Matchmaker™ GAL4 Two-Hybrid System 3, Clontech) using BamHI/PstI sites ...
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bioRxiv - Cell Biology 2024Quote: ... pEGFP-C1 and pLVX-IRES-Puro were purchased from ClonTech. Guide RNA sequences were cloned into pSpCas9(BB)-2A-GFP (pX458) ...
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bioRxiv - Cell Biology 2024Quote: The pGADT7 vector (pGADT7 AD, 7,987 bp) and T4 DNA (T4GT7, 166,644 bp) was purchased from Clontech (630442 ...
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bioRxiv - Cell Biology 2024Quote: ... was purchased from Clontech (630442; Clontech Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... transfected cells were treated with 500 nM of A/C Heterodimerizer (Takara #635057) at the onset of live imagining ...