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Citations for Takara Bio :
1401 - 1450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Ligation of the various components was undertaken using an In-Fusion cloning kit (Takara Bio-Europe, Paris, France). The ligation reaction was transformed into Stellar competent cells as per manufacturer protocols ...
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bioRxiv - Genetics 2024Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a PrimeScript RT reagent Kit (Cat. no. RR047B, TaKaRa) and was used as a template to perform qPCR in TB green-based qPCR instrument (CFX-96 ...
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bioRxiv - Genetics 2024Quote: ... cDNA was synthesized from total RNA using a PrimeScript RT reagent Kit (TaKaRa, Japan) and was used as a template to perform qPCR in SYRB green-based qPCR instrument (CFX-96 ...
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bioRxiv - Genetics 2024Quote: ... and total RNA was extracted and subsequently used to synthesize cDNA using the TaKaRa RNA PCRTM Kit (AMV) Ver.3.0 (TaKaRa). Quantitative real-time PCR (qPCR ...
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bioRxiv - Genetics 2024Quote: ... Aliquots of these samples were diluted 1:10 and 1:100 on two plates (Takara Bio): (1 ...
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bioRxiv - Genetics 2024Quote: ... Aliquots of these samples were diluted 1:10 and 1:100 on three plates (Takara Bio): (1 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µl of a 1:5 cDNA dilution was used together with forward and reverse primers per each mRNA (2 µM; Supp. Table 1) and 5 µl of SYBR® Premix-Ex-Taq (Tli RNase H Plus, Takara) in a 10 µl reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... amounts ranging from 10 picograms to 10 nanograms of total RNA were employed using the SMART-seq v4 Ultra Low Input RNA Kit from Takara Bio ...
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bioRxiv - Developmental Biology 2024Quote: ... 1:2000 monoclonal anti-mCherry (Takara/Clontech #632543), 1:1000 polyclonal anti-mCherry (Chromotek pabr1 ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplification was done with CloneAmp HiFi (639298, Takara Bio). Primers were designed using the Takara website (https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and transformed into Stellar competent cells (Takara, Cat. No. 636763). For cell entry validation mutants ...
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bioRxiv - Developmental Biology 2024Quote: ... 1:2000 monoclonal anti-mCherry (Takara/Clontech #632543), 1:1000 polyclonal anti-mCherry (Chromotek pabr1 ...
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bioRxiv - Developmental Biology 2024Quote: ... The fragment was then inserted in the pCAGEN-IRES-tomato with the In-Fusion® HD Cloning Kit (639649, Takara bio) according to manufacturer recommendations to generate pCAGEN-ATP1A3-WT-IRES-Tomato (named ATP1A3-WT) ...
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bioRxiv - Developmental Biology 2024Quote: ... The SMART-seq mRNA kit (Takara, San Jose, California, USA) was used to construct cDNA ...
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bioRxiv - Developmental Biology 2024Quote: Quantitative PCR reactions were performed using the Takara TP-850 thermal cycler (Takara Bio, Japan) and THUNDERBIRD® SYBR™ qPCR Mix (Toyobo ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We concentrated the supernatant by adding Lenti-X Concentrator (Takara, Cat. No. 631232) at a ratio of 1:3 ...
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bioRxiv - Genetics 2024Quote: ... using In-Fusion Snap Assembly (Takara, 638949). To obtain regulatory elements with variant alleles not present in the genomic DNA used for amplification ...
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bioRxiv - Developmental Biology 2024Quote: ... Blastocysts were transferred to DO ESC culture medium (Cellartis 3i mES/iPSC medium, Takara Bio, catalog # 1181722446) in a cell culture dish covered with mouse embryonic fibroblast (MEF ...
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bioRxiv - Developmental Biology 2024Quote: ... Human cDNA was purchased from Clontech. The cDNAs were used for PCR with rTaq polymerase (TaKaRa) ...
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bioRxiv - Developmental Biology 2024Quote: ... approximately 5 kb of 5’ and 3’ flanking sequences of the exoA gene were PCR amplified from w1118genomic DNA and cloned into the pattB vector (DGRC, stock 1420) using the in-fusion cloning method (Takara). The resulting construct was designated as pattB-exoA-mini ...
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bioRxiv - Developmental Biology 2024Quote: ... using 10% Tet-approved FBS (Takara) instead of calf serum ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by the E.coli xthA gene (coding Exonuclease III) cloned after the second FRT site via in-fusion cloning (Takara). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... except 10% tet-approved fetal bovine serum (Takara) was used in lieu of cosmic calf serum ...
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bioRxiv - Developmental Biology 2024Quote: ... Gastruloids were generated as described before (11,14) with the following adaptations: N2B27 medium (Takara) was passed through a .22 µm filter before use and 600 cells were used for aggregation instead of 300.
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bioRxiv - Developmental Biology 2024Quote: ... using the same paired read adapter oligonucleotides described by 99 were prepared from the ChIP and Input DNAs on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step ...
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bioRxiv - Genetics 2024Quote: ... Esrp1 cDNA was cloned into the pcDNA3.1 backbone containing a CMV promoter and SV40 polyA tailing sequence for expression in mammalian cells using the In-Fusion HD Cloning Kit (Clontech) to generate the pcDNA3.1-esrp1-mCherry plasmid ...
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bioRxiv - Genetics 2024Quote: ... cDNA was generated from 1μg of total RNA using the PrimeScriptTM RT reagent kit (TaKaRa, Shiga, Japan) for the quantitation of rRNA by quantitative real-time PCR ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from the leaves using the Acid Phenol Guanidium Chloroform method (Chomczynski and Sacchi 2006) and was then treated with 10 U of DNase I (Takara Bio Inc., Kyoto, Japan). poly(A)+ RNA was separated and purified from total RNA using an oligo(dT ...
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bioRxiv - Genetics 2024Quote: ... was cloned into the linearized DNA template vector (Takara Bio USA). The plasmid vectors were purified by a QIAprep spin miniprep kit (QIAGEN) ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified PCR fragment was ligated into pMD20-T vector (Takara Bio). The Cas9 plasmid was used as a template for in vitro transcription using mMESSAGE mMACHINE T7 Ultra Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... quantitative real-time PCR (RT-qPCR) was performed using the cDNA libraries as the template and a TB Green Premix Ex Taq kit (Takara) in a CFX96 real-time PCR machine (BioRad) ...
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bioRxiv - Genetics 2024Quote: ... we used a Matchmaker Gold Yeast Two-Hybrid System (Takara Bio Inc. ...
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bioRxiv - Developmental Biology 2024Quote: ... and used in quantitative real time reverse transcriptase PCR (qRT-PCR) with Takara SYBR Premix Ex Taq (Tli RNaseH Plus) (Takara Bio USA Inc, Mountain View, CA, USA) and LC480 (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... The RT-qPCR amplifications were performed using 2× SYBY Premix Ex TapTM II (Takara, China) as described previously ...
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bioRxiv - Developmental Biology 2024Quote: ... Blastocysts were then transferred to embryonic stem (ES) cell culture medium (Cellartis 3i mES/iPSC medium, Takara Bio, catalog # 1181722446) in a cell culture dish covered with mouse embryonic fibroblast (MEF ...
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bioRxiv - Genetics 2024Quote: ... and 1 U TaKaRa LA Taq (Takara Bio Inc., Kyoto, Japan) was prepared ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s specifications and SMART-Seq v4 Ultra Low Input RNA Kit (TAKARA) was used to synthesize cDNA from messenger RNA ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed using TB Green Premix Ex Taq (Takara). The ΔCt method using Gapdh as a reference gene was used to analyse relative expression and the 2−ΔΔCt (Livak ...
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bioRxiv - Developmental Biology 2024Quote: ... paired-end library preparation was performed using the SMART-Seq HT kit (Takara, Cat. No. 634437) followed by Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Genomics 2024Quote: ... and an hph gene containing plasmid (p3xFLAG::hph::LoxP) (62) using LA Taq (cat# RR002M; Takara), and gel purified ...
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bioRxiv - Genomics 2024Quote: ... we used a 2:1 molar ratio of insert to SalI/AgeI-digested pTYC10 (125 ng of pPromALB_STARRseq plasmid; Table S1E) in a single 10 μl In-Fusion HD reaction (Clontech, cat. # 639650) via the homology arms ...
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bioRxiv - Plant Biology 2024Quote: ... Bio-RAD CFX96 system was applied to perform qRT-PCR with TB Green Premix Ex Taq (Takara). Relative expression level calculation method was follow as described.
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bioRxiv - Plant Biology 2024Quote: ... Rwt4Ca and its variant proteins were cloned into the prey vector pGADT7 (Takara, #630442) while AvrPwt4 and VirPwt4 were cloned into the bait vector pGBKT7 (Takara ...
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bioRxiv - Microbiology 2024Quote: ... to make each ORF compatible for In-Fusion cloning (Takara Bio). Sequences were assembled into pcDNA3.1 vector encoding VC155 or VN173 on the N- or C-terminus ...
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bioRxiv - Microbiology 2024Quote: ... The rNiV TC-tr minigenome encoding VC155 was constructed by subcloning the VC155 sequence from the pcDNA3.1 VC155-2xLinker-NiV-M construct and assembling it into restriction-digested rNiV TC-tr minigenome by In-Fusion cloning (Takara Bio) approaches ...
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bioRxiv - Microbiology 2024Quote: ... CDS cDNA was then used as template for PCR with HiFi PCR mix (Takara Bio) to make each ORF compatible for In-Fusion cloning (Takara Bio) ...
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bioRxiv - Neuroscience 2024Quote: ... Infusion cloning (Takara-Bio In-Fusion HD cloning Kit; 639650) was used to clone in EF1-alpha promoter generating a pAAV.U6.shRLuc.EF1-α.ZsGreen.SV40 plasmid and express ZsGreen under EF1-α promoter (Table 2) ...
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bioRxiv - Cell Biology 2024Quote: ... Constructs that express wrmScarlet-tagged V-ATPase components with gfp::unc-54 3’ UTR were generated using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). The primers were listed in Extended Data Table 3.
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bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis was carried out to create the 5S and 12S mutants using PrimeSTAR Max DNA polymerase (Takara-bio). All plasmids were sequenced and verified prior to the transformation of DH10Bac ...
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bioRxiv - Cell Biology 2024Quote: Lenti-X 293Ts (Takara) were transfected with the Guide-it library (Takara ...